Klebsiella phage ST11-VIM1phi8.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482MDY1|A0A482MDY1_9CAUD Arc family DNA binding protein OS=Klebsiella phage ST11-VIM1phi8.1 OX=2555910 PE=4 SV=1
MM1 pKa = 8.07ADD3 pKa = 4.79PITAADD9 pKa = 3.57VQAFLGEE16 pKa = 4.34LGYY19 pKa = 10.55SIPAALLDD27 pKa = 5.02PILCVVNKK35 pKa = 10.3IIPCLDD41 pKa = 2.88GAGYY45 pKa = 9.94DD46 pKa = 3.33EE47 pKa = 4.93CTAKK51 pKa = 10.82LILMYY56 pKa = 10.52AAALMATSSGARR68 pKa = 11.84RR69 pKa = 11.84IKK71 pKa = 10.75SQGAPSGASRR81 pKa = 11.84SFDD84 pKa = 3.58YY85 pKa = 11.32GDD87 pKa = 5.93DD88 pKa = 4.56GITWLRR94 pKa = 11.84DD95 pKa = 3.31SLAKK99 pKa = 10.37LDD101 pKa = 3.96TSGCTSEE108 pKa = 5.38LPISAGNTVGLFMVVGDD125 pKa = 4.24CC126 pKa = 5.3

Molecular weight: 13.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482MFD5|A0A482MFD5_9CAUD Arc family DNA binding protein OS=Klebsiella phage ST11-VIM1phi8.1 OX=2555910 PE=4 SV=1
MM1 pKa = 7.42ARR3 pKa = 11.84VSVQQRR9 pKa = 11.84LPRR12 pKa = 11.84TFTRR16 pKa = 11.84VWVITDD22 pKa = 4.0AGQQTTAYY30 pKa = 9.48VKK32 pKa = 11.14SNGEE36 pKa = 3.63WFINCDD42 pKa = 3.7RR43 pKa = 11.84IRR45 pKa = 11.84ATGAVVLRR53 pKa = 11.84WRR55 pKa = 11.84DD56 pKa = 3.39DD57 pKa = 3.2

Molecular weight: 6.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

13090

36

1148

198.3

22.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.298 ± 0.662

1.314 ± 0.175

5.814 ± 0.265

6.639 ± 0.304

3.331 ± 0.221

6.761 ± 0.221

1.719 ± 0.169

5.439 ± 0.158

5.936 ± 0.331

7.701 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.62 ± 0.187

4.423 ± 0.227

3.713 ± 0.319

4.362 ± 0.424

6.234 ± 0.319

6.348 ± 0.259

5.882 ± 0.244

6.486 ± 0.267

1.803 ± 0.151

3.178 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski