Canis familiaris papillomavirus 15
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L0CKP3|L0CKP3_9PAPI Protein E7 OS=Canis familiaris papillomavirus 15 OX=1272519 GN=E7 PE=3 SV=1
MM1 pKa = 7.31 IGKK4 pKa = 9.33 GATLKK9 pKa = 10.95 DD10 pKa = 3.3 IVLEE14 pKa = 4.16 EE15 pKa = 4.24 QPCPYY20 pKa = 10.65 DD21 pKa = 3.73 EE22 pKa = 5.35 LYY24 pKa = 10.79 CDD26 pKa = 4.09 EE27 pKa = 4.89 EE28 pKa = 4.62 LPPEE32 pKa = 4.39 DD33 pKa = 4.17 EE34 pKa = 4.72 EE35 pKa = 4.24 EE36 pKa = 4.66 AEE38 pKa = 5.6 QEE40 pKa = 4.4 TQDD43 pKa = 3.21 IAPYY47 pKa = 10.13 RR48 pKa = 11.84 IQTLCGHH55 pKa = 6.89 CDD57 pKa = 2.69 RR58 pKa = 11.84 GIRR61 pKa = 11.84 LVVLSTPHH69 pKa = 7.61 GIRR72 pKa = 11.84 TLEE75 pKa = 4.51 DD76 pKa = 3.69 LLLSCIGVCCPEE88 pKa = 4.2 CASRR92 pKa = 11.84 RR93 pKa = 11.84 WRR95 pKa = 11.84 FGRR98 pKa = 11.84 AQHH101 pKa = 6.55 GGG103 pKa = 3.34
Molecular weight: 11.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.315
IPC2_protein 4.622
IPC_protein 4.495
Toseland 4.368
ProMoST 4.596
Dawson 4.444
Bjellqvist 4.584
Wikipedia 4.291
Rodwell 4.342
Grimsley 4.279
Solomon 4.431
Lehninger 4.393
Nozaki 4.558
DTASelect 4.673
Thurlkill 4.368
EMBOSS 4.317
Sillero 4.609
Patrickios 2.015
IPC_peptide 4.444
IPC2_peptide 4.609
IPC2.peptide.svr19 4.537
Protein with the highest isoelectric point:
>tr|L0CKP3|L0CKP3_9PAPI Protein E7 OS=Canis familiaris papillomavirus 15 OX=1272519 GN=E7 PE=3 SV=1
MM1 pKa = 7.01 EE2 pKa = 4.74 TLSKK6 pKa = 10.58 RR7 pKa = 11.84 LNALQDD13 pKa = 3.6 ALLNLYY19 pKa = 10.05 EE20 pKa = 4.26 EE21 pKa = 5.25 ASDD24 pKa = 3.56 EE25 pKa = 4.14 LQRR28 pKa = 11.84 QVDD31 pKa = 2.97 HH32 pKa = 6.56 WKK34 pKa = 10.06 ILRR37 pKa = 11.84 QEE39 pKa = 4.03 NVLLHH44 pKa = 5.48 YY45 pKa = 10.24 ARR47 pKa = 11.84 KK48 pKa = 10.05 SGVLRR53 pKa = 11.84 VGMQPVPPLQVSAEE67 pKa = 4.17 RR68 pKa = 11.84 AKK70 pKa = 10.69 HH71 pKa = 5.48 AIEE74 pKa = 3.95 MQIVLQSLCDD84 pKa = 3.87 SQWASEE90 pKa = 4.02 TWTLADD96 pKa = 3.66 TSRR99 pKa = 11.84 EE100 pKa = 3.94 LWTAAPQRR108 pKa = 11.84 CFKK111 pKa = 10.64 KK112 pKa = 9.58 GACIVEE118 pKa = 4.32 VIYY121 pKa = 11.16 DD122 pKa = 4.33 GDD124 pKa = 4.35 PDD126 pKa = 3.86 NCMQYY131 pKa = 8.85 TLWSRR136 pKa = 11.84 VYY138 pKa = 10.14 YY139 pKa = 10.35 QDD141 pKa = 6.12 GSDD144 pKa = 3.24 TWQMSRR150 pKa = 11.84 SQVDD154 pKa = 3.56 CVGIWFWDD162 pKa = 4.06 CEE164 pKa = 4.03 QKK166 pKa = 10.54 RR167 pKa = 11.84 YY168 pKa = 8.81 YY169 pKa = 11.12 VKK171 pKa = 10.49 FANDD175 pKa = 3.15 AARR178 pKa = 11.84 YY179 pKa = 6.19 GTTGTWEE186 pKa = 3.85 VVYY189 pKa = 10.89 NGEE192 pKa = 4.67 TVTSSDD198 pKa = 3.87 PVTSTTPPTDD208 pKa = 3.37 PCGEE212 pKa = 3.83 APVYY216 pKa = 10.19 HH217 pKa = 6.83 RR218 pKa = 11.84 RR219 pKa = 11.84 LSPHH223 pKa = 6.34 GPSAGDD229 pKa = 3.37 ANPPIEE235 pKa = 4.88 RR236 pKa = 11.84 GGGDD240 pKa = 3.54 RR241 pKa = 11.84 TPASTPEE248 pKa = 3.78 TATQVSSTYY257 pKa = 10.0 TSPLPDD263 pKa = 3.41 TTSPSPRR270 pKa = 11.84 PLGEE274 pKa = 3.96 VEE276 pKa = 3.83 GDD278 pKa = 3.03 RR279 pKa = 11.84 GGGGGRR285 pKa = 11.84 GATAEE290 pKa = 4.22 PVGSSPLAAAAIAATPVSVPRR311 pKa = 11.84 VPLTPRR317 pKa = 11.84 KK318 pKa = 9.2 RR319 pKa = 11.84 PRR321 pKa = 11.84 GRR323 pKa = 11.84 GPSEE327 pKa = 3.88 PPCRR331 pKa = 11.84 RR332 pKa = 11.84 QPPPSPGEE340 pKa = 3.93 PEE342 pKa = 4.07 PAVDD346 pKa = 3.78 PGLVPVCGRR355 pKa = 11.84 PRR357 pKa = 11.84 TPPITPQGGQPSPLTVPVAEE377 pKa = 4.31 PRR379 pKa = 11.84 PVGSLTPPGGGLPPSPAPPAAEE401 pKa = 4.15 AARR404 pKa = 11.84 EE405 pKa = 3.96 PARR408 pKa = 11.84 RR409 pKa = 11.84 YY410 pKa = 9.16 PPAGARR416 pKa = 11.84 RR417 pKa = 11.84 LAVPGGHH424 pKa = 7.17 PNRR427 pKa = 11.84 PGDD430 pKa = 3.9 LLRR433 pKa = 11.84 EE434 pKa = 4.42 AGHH437 pKa = 7.02 LPQTAVVLCGPANALKK453 pKa = 10.3 CLRR456 pKa = 11.84 YY457 pKa = 9.59 RR458 pKa = 11.84 LQHH461 pKa = 5.29 HH462 pKa = 7.16 HH463 pKa = 5.65 GQKK466 pKa = 10.46 FLTVSTTWYY475 pKa = 8.59 WAGQGSSRR483 pKa = 11.84 LGPARR488 pKa = 11.84 IMLTFRR494 pKa = 11.84 DD495 pKa = 4.1 SQQQTDD501 pKa = 3.64 FFQSVRR507 pKa = 11.84 LPPSISRR514 pKa = 11.84 APSIFSVV521 pKa = 3.37
Molecular weight: 56.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.349
IPC2_protein 7.614
IPC_protein 7.454
Toseland 7.044
ProMoST 8.024
Dawson 8.126
Bjellqvist 8.595
Wikipedia 8.039
Rodwell 8.141
Grimsley 7.088
Solomon 8.185
Lehninger 8.214
Nozaki 8.785
DTASelect 8.258
Thurlkill 8.302
EMBOSS 8.346
Sillero 8.653
Patrickios 4.342
IPC_peptide 8.185
IPC2_peptide 7.892
IPC2.peptide.svr19 7.788
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2439
103
626
406.5
45.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.888 ± 0.571
2.296 ± 0.707
6.355 ± 0.502
6.068 ± 0.557
3.772 ± 0.54
7.913 ± 0.908
2.091 ± 0.232
3.567 ± 0.44
4.018 ± 1.046
8.282 ± 0.651
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.845 ± 0.523
3.321 ± 0.906
7.954 ± 1.386
4.182 ± 0.668
7.298 ± 0.811
7.257 ± 0.531
5.74 ± 0.539
6.929 ± 0.692
1.517 ± 0.294
2.706 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here