Equine coronavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Embecovirus; Betacoronavirus 1

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4WB36|R4WB36_9BETC 12.7 kDa accessory protein OS=Equine coronavirus OX=136187 GN=p12.7 PE=4 SV=1
MM1 pKa = 7.56LMADD5 pKa = 5.11FYY7 pKa = 11.63LADD10 pKa = 3.89TVWYY14 pKa = 8.76VGQIIFIVAICLLVIIVVVAFLATFKK40 pKa = 11.14LCIQLCGMCNTLVLSPSIYY59 pKa = 9.46VFNRR63 pKa = 11.84GRR65 pKa = 11.84QFYY68 pKa = 10.51EE69 pKa = 4.58LYY71 pKa = 10.42NQVKK75 pKa = 9.8PPVLNVDD82 pKa = 3.98DD83 pKa = 4.16VV84 pKa = 3.67

Molecular weight: 9.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4WKE3|R4WKE3_9BETC Nucleoprotein OS=Equine coronavirus OX=136187 GN=N PE=3 SV=1
MM1 pKa = 7.55SFTPGKK7 pKa = 9.79QSSSRR12 pKa = 11.84ASSGNRR18 pKa = 11.84SGNGILKK25 pKa = 9.42WADD28 pKa = 3.23QSDD31 pKa = 3.5QSTNFQTRR39 pKa = 11.84GRR41 pKa = 11.84RR42 pKa = 11.84AQPKK46 pKa = 7.97QTATSQPAGGNVVPYY61 pKa = 8.97YY62 pKa = 10.8SWFSGITQFQKK73 pKa = 10.82GKK75 pKa = 9.62EE76 pKa = 3.93FQFAEE81 pKa = 4.59GQGVPIAPGIPSTEE95 pKa = 3.96AKK97 pKa = 10.3GYY99 pKa = 8.63WYY101 pKa = 10.34RR102 pKa = 11.84HH103 pKa = 4.43NRR105 pKa = 11.84RR106 pKa = 11.84SFKK109 pKa = 10.37TADD112 pKa = 3.13GNQRR116 pKa = 11.84QLLPRR121 pKa = 11.84WYY123 pKa = 9.8FYY125 pKa = 11.56YY126 pKa = 10.78LGTGPHH132 pKa = 6.64AKK134 pKa = 9.43VQYY137 pKa = 8.17GTNIDD142 pKa = 3.63GVFWVANKK150 pKa = 9.9QADD153 pKa = 4.06VNTPADD159 pKa = 3.71VVDD162 pKa = 4.88RR163 pKa = 11.84DD164 pKa = 3.84PSSDD168 pKa = 3.16EE169 pKa = 4.8AIPTRR174 pKa = 11.84FPPGTVLPQGYY185 pKa = 9.38YY186 pKa = 9.56IEE188 pKa = 4.79GSGRR192 pKa = 11.84SVSNSRR198 pKa = 11.84STSRR202 pKa = 11.84ASSRR206 pKa = 11.84ASSAGSRR213 pKa = 11.84SRR215 pKa = 11.84ANSGTRR221 pKa = 11.84TSTSGVTPDD230 pKa = 3.74MADD233 pKa = 3.28QIASLVLAKK242 pKa = 10.41LGKK245 pKa = 10.07DD246 pKa = 2.95ATKK249 pKa = 9.49PQQVTKK255 pKa = 9.36QTAKK259 pKa = 10.33EE260 pKa = 4.03VRR262 pKa = 11.84QKK264 pKa = 10.72ILNKK268 pKa = 9.23PRR270 pKa = 11.84QKK272 pKa = 10.46RR273 pKa = 11.84SPNKK277 pKa = 9.02QCTVQQCFGKK287 pKa = 10.48RR288 pKa = 11.84GPNQNFGGAEE298 pKa = 3.96MLKK301 pKa = 10.59LGTSDD306 pKa = 3.38PQFPILAEE314 pKa = 4.05LAPTAGAFFFGSRR327 pKa = 11.84LEE329 pKa = 4.03LAKK332 pKa = 10.64VQNLSGSFDD341 pKa = 3.63EE342 pKa = 4.67PQKK345 pKa = 11.02DD346 pKa = 4.14VYY348 pKa = 9.8EE349 pKa = 3.97LRR351 pKa = 11.84YY352 pKa = 10.38NGAIRR357 pKa = 11.84FDD359 pKa = 3.51STLPGFEE366 pKa = 4.83TIMKK370 pKa = 9.51VLNEE374 pKa = 4.23NLNAYY379 pKa = 7.25QQQDD383 pKa = 3.77DD384 pKa = 4.49GTNMSPKK391 pKa = 9.33PQRR394 pKa = 11.84QRR396 pKa = 11.84GQKK399 pKa = 10.01KK400 pKa = 9.24GGEE403 pKa = 4.15NEE405 pKa = 4.34DD406 pKa = 3.5VSVAAPKK413 pKa = 10.72SRR415 pKa = 11.84VQQNKK420 pKa = 9.07SRR422 pKa = 11.84EE423 pKa = 4.15LTAEE427 pKa = 4.82DD428 pKa = 3.74ISLLKK433 pKa = 11.01QMDD436 pKa = 4.01DD437 pKa = 4.38PLTEE441 pKa = 5.41DD442 pKa = 3.29NSEE445 pKa = 3.98MM446 pKa = 4.58

Molecular weight: 49.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

14590

84

7117

1459.0

163.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.552 ± 0.315

3.53 ± 0.295

5.97 ± 0.448

3.619 ± 0.157

5.517 ± 0.163

5.86 ± 0.344

1.549 ± 0.151

5.422 ± 0.354

5.798 ± 0.587

9.191 ± 0.647

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.207 ± 0.168

5.469 ± 0.581

3.276 ± 0.474

3.626 ± 0.29

3.427 ± 0.284

7.361 ± 0.22

5.696 ± 0.296

9.527 ± 0.753

1.295 ± 0.071

5.106 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski