Rusa timorensis papillomavirus type 1
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A109PMF8|A0A109PMF8_9PAPI Major capsid protein L1 OS=Rusa timorensis papillomavirus type 1 OX=1776868 GN=L1 PE=3 SV=1
MM1 pKa = 7.0 CAEE4 pKa = 4.76 MIGEE8 pKa = 4.3 SATIQDD14 pKa = 5.31 IILQDD19 pKa = 3.29 IDD21 pKa = 5.16 SIDD24 pKa = 3.95 LACHH28 pKa = 5.32 EE29 pKa = 4.52 TLSSEE34 pKa = 4.29 VEE36 pKa = 3.79 QRR38 pKa = 11.84 RR39 pKa = 11.84 PTVYY43 pKa = 9.56 PYY45 pKa = 10.79 KK46 pKa = 10.37 IVTRR50 pKa = 11.84 CGQCEE55 pKa = 3.92 TSICLYY61 pKa = 10.65 VGSTQIGIVTLQSQLAEE78 pKa = 4.55 DD79 pKa = 4.2 LCILCGNCGRR89 pKa = 11.84 QNFPNGRR96 pKa = 11.84 KK97 pKa = 9.56 KK98 pKa = 10.74 SS99 pKa = 3.63
Molecular weight: 10.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.717
IPC2_protein 4.889
IPC_protein 4.685
Toseland 4.546
ProMoST 4.787
Dawson 4.635
Bjellqvist 4.787
Wikipedia 4.495
Rodwell 4.533
Grimsley 4.457
Solomon 4.622
Lehninger 4.584
Nozaki 4.749
DTASelect 4.876
Thurlkill 4.558
EMBOSS 4.52
Sillero 4.8
Patrickios 0.693
IPC_peptide 4.635
IPC2_peptide 4.8
IPC2.peptide.svr19 4.751
Protein with the highest isoelectric point:
>tr|A0A0X9K182|A0A0X9K182_9PAPI Replication protein E1 OS=Rusa timorensis papillomavirus type 1 OX=1776868 GN=E1 PE=3 SV=1
MM1 pKa = 7.35 ATLNDD6 pKa = 4.23 RR7 pKa = 11.84 FDD9 pKa = 4.89 ALQQSQLDD17 pKa = 3.99 IIEE20 pKa = 4.91 AEE22 pKa = 4.3 PGTLEE27 pKa = 4.66 DD28 pKa = 5.55 QIFYY32 pKa = 10.43 WDD34 pKa = 4.17 LLRR37 pKa = 11.84 QEE39 pKa = 5.61 RR40 pKa = 11.84 VLQYY44 pKa = 8.74 YY45 pKa = 9.91 ARR47 pKa = 11.84 KK48 pKa = 9.7 KK49 pKa = 9.21 GYY51 pKa = 9.52 KK52 pKa = 9.19 RR53 pKa = 11.84 LGMLPLPTLQISEE66 pKa = 4.38 AAAKK70 pKa = 9.59 EE71 pKa = 4.11 AIAMTLHH78 pKa = 6.63 LSSLKK83 pKa = 10.14 EE84 pKa = 3.56 SSYY87 pKa = 11.89 ANEE90 pKa = 3.76 RR91 pKa = 11.84 WTLQDD96 pKa = 3.11 TSIVTFEE103 pKa = 4.78 SPPQFTFKK111 pKa = 10.48 KK112 pKa = 10.13 GPVAVKK118 pKa = 10.32 LSFDD122 pKa = 4.07 GNPDD126 pKa = 3.01 NAVRR130 pKa = 11.84 HH131 pKa = 5.02 TLWRR135 pKa = 11.84 DD136 pKa = 2.74 IYY138 pKa = 11.54 YY139 pKa = 10.49 LDD141 pKa = 6.46 DD142 pKa = 5.24 DD143 pKa = 4.42 DD144 pKa = 4.39 QWRR147 pKa = 11.84 KK148 pKa = 7.82 TYY150 pKa = 11.18 SDD152 pKa = 3.12 VDD154 pKa = 3.64 SRR156 pKa = 11.84 GIYY159 pKa = 8.97 YY160 pKa = 10.06 WSGDD164 pKa = 3.44 SKK166 pKa = 11.34 VYY168 pKa = 10.4 YY169 pKa = 10.8 VEE171 pKa = 4.85 FKK173 pKa = 11.03 DD174 pKa = 4.63 DD175 pKa = 3.51 ASLYY179 pKa = 10.44 SLTGQWEE186 pKa = 3.99 VTYY189 pKa = 10.85 NKK191 pKa = 8.97 KK192 pKa = 8.31 TFSSSIATSSTNEE205 pKa = 3.51 EE206 pKa = 4.64 DD207 pKa = 3.98 GPVPISSDD215 pKa = 3.49 EE216 pKa = 4.15 EE217 pKa = 4.4 GTDD220 pKa = 3.36 GHH222 pKa = 5.72 GQEE225 pKa = 5.4 HH226 pKa = 6.26 EE227 pKa = 4.14 RR228 pKa = 11.84 SPNRR232 pKa = 11.84 SDD234 pKa = 3.41 TEE236 pKa = 4.06 EE237 pKa = 5.25 GEE239 pKa = 4.4 EE240 pKa = 4.16 TGGEE244 pKa = 3.94 ASEE247 pKa = 4.45 KK248 pKa = 10.61 ASISGSVRR256 pKa = 11.84 SIRR259 pKa = 11.84 TARR262 pKa = 11.84 SPKK265 pKa = 9.21 AHH267 pKa = 6.6 SNPRR271 pKa = 11.84 SKK273 pKa = 10.68 SRR275 pKa = 11.84 SRR277 pKa = 11.84 SRR279 pKa = 11.84 SRR281 pKa = 11.84 SRR283 pKa = 11.84 SSTRR287 pKa = 11.84 GWTRR291 pKa = 11.84 ARR293 pKa = 11.84 QTSSSGSRR301 pKa = 11.84 SRR303 pKa = 11.84 SRR305 pKa = 11.84 SGSRR309 pKa = 11.84 TRR311 pKa = 11.84 ARR313 pKa = 11.84 SWRR316 pKa = 11.84 RR317 pKa = 11.84 SPGHH321 pKa = 6.57 RR322 pKa = 11.84 GRR324 pKa = 11.84 GRR326 pKa = 11.84 GRR328 pKa = 11.84 PPGSGRR334 pKa = 11.84 RR335 pKa = 11.84 QGGAGSRR342 pKa = 11.84 PGAGSGAGGRR352 pKa = 11.84 PGLRR356 pKa = 11.84 SRR358 pKa = 11.84 SRR360 pKa = 11.84 DD361 pKa = 3.11 TRR363 pKa = 11.84 AGSGLPNRR371 pKa = 11.84 PSASPRR377 pKa = 11.84 AEE379 pKa = 3.53 PVRR382 pKa = 11.84 VLEE385 pKa = 4.16 GSIPRR390 pKa = 11.84 PSSYY394 pKa = 11.14 GLRR397 pKa = 11.84 GSGGEE402 pKa = 4.3 TEE404 pKa = 5.14 APTDD408 pKa = 3.57 TSSLVSPPHH417 pKa = 6.06 LSPWFRR423 pKa = 11.84 DD424 pKa = 3.72 PPRR427 pKa = 11.84 APGRR431 pKa = 11.84 PPSGRR436 pKa = 11.84 HH437 pKa = 5.01 RR438 pKa = 11.84 RR439 pKa = 11.84 PAPTPEE445 pKa = 3.85 RR446 pKa = 11.84 RR447 pKa = 11.84 TTSPLARR454 pKa = 11.84 RR455 pKa = 11.84 KK456 pKa = 8.82 RR457 pKa = 11.84 TGSRR461 pKa = 11.84 STEE464 pKa = 4.01 RR465 pKa = 11.84 LHH467 pKa = 6.37 STPTSAKK474 pKa = 9.25 KK475 pKa = 10.17 DD476 pKa = 3.44 PPVIIVRR483 pKa = 11.84 GLPNQLKK490 pKa = 6.72 TWRR493 pKa = 11.84 YY494 pKa = 8.18 RR495 pKa = 11.84 LHH497 pKa = 6.3 NRR499 pKa = 11.84 RR500 pKa = 11.84 KK501 pKa = 9.76 KK502 pKa = 10.96 LPFLYY507 pKa = 10.7 LSTTFSWVTRR517 pKa = 11.84 KK518 pKa = 10.63 GGDD521 pKa = 3.22 RR522 pKa = 11.84 LSSTRR527 pKa = 11.84 MLVAFASYY535 pKa = 10.95 SDD537 pKa = 3.06 RR538 pKa = 11.84 SYY540 pKa = 11.45 FLRR543 pKa = 11.84 HH544 pKa = 4.92 VVFPPGVTFSLGYY557 pKa = 10.24 FYY559 pKa = 11.49 GLL561 pKa = 3.62
Molecular weight: 62.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.78
IPC_protein 10.745
Toseland 10.672
ProMoST 10.496
Dawson 10.804
Bjellqvist 10.555
Wikipedia 11.052
Rodwell 10.891
Grimsley 10.862
Solomon 10.935
Lehninger 10.891
Nozaki 10.643
DTASelect 10.555
Thurlkill 10.687
EMBOSS 11.096
Sillero 10.73
Patrickios 10.526
IPC_peptide 10.935
IPC2_peptide 9.487
IPC2.peptide.svr19 8.544
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2437
99
585
406.2
45.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.032 ± 0.783
2.134 ± 0.933
6.483 ± 0.425
5.868 ± 0.381
4.637 ± 0.603
7.591 ± 0.783
2.093 ± 0.255
4.432 ± 0.83
4.76 ± 0.974
7.92 ± 0.597
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.395 ± 0.21
3.57 ± 0.427
6.319 ± 0.637
3.816 ± 0.391
7.427 ± 1.33
8.33 ± 1.421
6.155 ± 0.558
5.909 ± 0.595
1.477 ± 0.314
3.652 ± 0.34
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here