Yichang Insect virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Goukovirus; Yichang insect goukovirus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KXZ2|A0A0B5KXZ2_9VIRU Replicase OS=Yichang Insect virus OX=1608144 GN=L PE=4 SV=1
MM1 pKa = 7.09STRR4 pKa = 11.84TTGTYY9 pKa = 10.5PLVQDD14 pKa = 3.55MLKK17 pKa = 10.76ARR19 pKa = 11.84VPNTNLTLACLLAIGLSGCGGGVGMAQAEE48 pKa = 4.41IAASVPYY55 pKa = 9.76GAHH58 pKa = 6.44EE59 pKa = 4.76GMTQAACPDD68 pKa = 3.58EE69 pKa = 5.24KK70 pKa = 11.1LLSNKK75 pKa = 8.68WCGDD79 pKa = 2.92IPSTVNTCQPRR90 pKa = 11.84GGFIAQYY97 pKa = 9.85QWGDD101 pKa = 2.81KK102 pKa = 10.82YY103 pKa = 8.67YY104 pKa = 8.09TCSRR108 pKa = 11.84EE109 pKa = 4.45VICSPGEE116 pKa = 3.97YY117 pKa = 10.26LADD120 pKa = 4.45DD121 pKa = 4.2CTCALLIKK129 pKa = 10.24DD130 pKa = 4.99CYY132 pKa = 10.85HH133 pKa = 6.69EE134 pKa = 4.44AQNCRR139 pKa = 11.84VPKK142 pKa = 9.03LTKK145 pKa = 10.29LGSGTCTEE153 pKa = 4.86KK154 pKa = 10.63DD155 pKa = 3.01WKK157 pKa = 11.1ANEE160 pKa = 4.13PQFCSIGSPGEE171 pKa = 3.93GCNPIGVMRR180 pKa = 11.84EE181 pKa = 3.53LAYY184 pKa = 11.0VVDD187 pKa = 4.19STGKK191 pKa = 10.18SRR193 pKa = 11.84WITDD197 pKa = 3.6LNIEE201 pKa = 4.19EE202 pKa = 4.2ALIYY206 pKa = 10.48DD207 pKa = 3.75KK208 pKa = 11.34SEE210 pKa = 4.01SFIQDD215 pKa = 2.97KK216 pKa = 10.61HH217 pKa = 7.35VSLSGPVRR225 pKa = 11.84GNSAYY230 pKa = 10.26CDD232 pKa = 3.49HH233 pKa = 7.13FKK235 pKa = 11.07DD236 pKa = 5.31LPNCYY241 pKa = 10.44ASIASEE247 pKa = 3.75NSPYY251 pKa = 10.47RR252 pKa = 11.84IFRR255 pKa = 11.84SNGAIPHH262 pKa = 6.0IHH264 pKa = 7.51LDD266 pKa = 3.65TCSSPVYY273 pKa = 10.2CYY275 pKa = 10.59GYY277 pKa = 8.72KK278 pKa = 8.03TTLVYY283 pKa = 8.38THH285 pKa = 6.85KK286 pKa = 8.33TTEE289 pKa = 4.3LKK291 pKa = 10.63SSCSGCVVSCHH302 pKa = 4.85NTGVHH307 pKa = 5.82IVTNLEE313 pKa = 4.21GKK315 pKa = 8.68SHH317 pKa = 5.92IQVCGRR323 pKa = 11.84AEE325 pKa = 4.11CQMLDD330 pKa = 3.74TNSKK334 pKa = 10.04ILDD337 pKa = 3.78LPRR340 pKa = 11.84SFHH343 pKa = 7.28SRR345 pKa = 11.84TTDD348 pKa = 2.68EE349 pKa = 4.89RR350 pKa = 11.84IRR352 pKa = 11.84VTIHH356 pKa = 6.18SEE358 pKa = 3.92DD359 pKa = 3.74RR360 pKa = 11.84LVSYY364 pKa = 11.12DD365 pKa = 4.03NFTACPVVPVCEE377 pKa = 5.55AIDD380 pKa = 3.58CTFCSDD386 pKa = 3.33HH387 pKa = 6.74LLNPTCYY394 pKa = 10.5SKK396 pKa = 11.35LDD398 pKa = 3.47WVLTLVFLYY407 pKa = 11.08VLMVLTGLALTCAIPIFRR425 pKa = 11.84VLWNLVSFMIKK436 pKa = 10.24LFWKK440 pKa = 5.9MTKK443 pKa = 9.56MMLRR447 pKa = 11.84MSGRR451 pKa = 11.84AAGRR455 pKa = 11.84AKK457 pKa = 10.35QFADD461 pKa = 3.89DD462 pKa = 4.93DD463 pKa = 4.95SIEE466 pKa = 4.31LRR468 pKa = 11.84PASIVRR474 pKa = 11.84EE475 pKa = 3.89PLINSRR481 pKa = 11.84PARR484 pKa = 11.84QNKK487 pKa = 9.84AGIRR491 pKa = 11.84LPAGLVIIMILLPLVVCKK509 pKa = 10.47APCSSVVVDD518 pKa = 3.67TVMTEE523 pKa = 3.64SCTLKK528 pKa = 10.54GDD530 pKa = 4.08SYY532 pKa = 11.12HH533 pKa = 7.13CSISSINEE541 pKa = 3.78VPLISYY547 pKa = 8.81DD548 pKa = 3.4QTTCLMYY555 pKa = 9.17LTPGGQTLGQLQITPLDD572 pKa = 3.41ISFKK576 pKa = 10.26CSKK579 pKa = 9.52QALYY583 pKa = 8.64HH584 pKa = 5.56TRR586 pKa = 11.84DD587 pKa = 3.26FEE589 pKa = 4.23MLSDD593 pKa = 3.61HH594 pKa = 6.62VVRR597 pKa = 11.84CPRR600 pKa = 11.84SGPCTEE606 pKa = 4.72DD607 pKa = 2.62WCHH610 pKa = 6.8SVTTDD615 pKa = 3.03TDD617 pKa = 3.84VPDD620 pKa = 4.35LPKK623 pKa = 10.18ISYY626 pKa = 9.01PSHH629 pKa = 5.88QSCKK633 pKa = 10.35LGEE636 pKa = 4.29ACWGNGCFFCTSSCHH651 pKa = 5.84VIRR654 pKa = 11.84YY655 pKa = 5.98YY656 pKa = 11.15ARR658 pKa = 11.84PKK660 pKa = 10.0TDD662 pKa = 3.59TIYY665 pKa = 11.05EE666 pKa = 4.35LYY668 pKa = 10.44SCPKK672 pKa = 8.47WDD674 pKa = 3.86PSGSFHH680 pKa = 6.97FLWEE684 pKa = 4.36STSGNTEE691 pKa = 3.82TTISLIHH698 pKa = 6.85GEE700 pKa = 4.35TKK702 pKa = 10.55SISSDD707 pKa = 3.01MSVTLDD713 pKa = 3.53VNIQSKK719 pKa = 10.79LPILSKK725 pKa = 9.89TFISDD730 pKa = 3.27GRR732 pKa = 11.84RR733 pKa = 11.84VALSDD738 pKa = 3.68SSFAGQPVAGQLGQVQCHH756 pKa = 5.08SAEE759 pKa = 4.3KK760 pKa = 10.27ASKK763 pKa = 10.14LQSCTMAPNICVCRR777 pKa = 11.84PEE779 pKa = 4.45QSDD782 pKa = 4.17DD783 pKa = 3.81GCDD786 pKa = 3.42CSQVFIDD793 pKa = 5.66RR794 pKa = 11.84IFSSSAVLPVNVEE807 pKa = 3.74NHH809 pKa = 6.4HH810 pKa = 7.89IEE812 pKa = 4.23MNRR815 pKa = 11.84DD816 pKa = 3.03TPYY819 pKa = 11.25LKK821 pKa = 9.88TPAFGSGHH829 pKa = 5.37IKK831 pKa = 10.07VRR833 pKa = 11.84SSMKK837 pKa = 10.56LIGTAVPAEE846 pKa = 4.26SCSVNLIKK854 pKa = 10.56ISGCYY859 pKa = 9.65SCVSGARR866 pKa = 11.84LSYY869 pKa = 10.12SCSTEE874 pKa = 3.5KK875 pKa = 10.56HH876 pKa = 5.04HH877 pKa = 6.79TVVASCYY884 pKa = 10.71GDD886 pKa = 2.61IHH888 pKa = 7.93LILEE892 pKa = 4.59CDD894 pKa = 3.15NSGVEE899 pKa = 4.0RR900 pKa = 11.84EE901 pKa = 4.31VRR903 pKa = 11.84FQSPTPLITLQCTTACSKK921 pKa = 10.37MGFSLSGTLVRR932 pKa = 11.84MAQPDD937 pKa = 3.88LSGQGHH943 pKa = 6.77INLVPGAAKK952 pKa = 10.18EE953 pKa = 4.18GAIEE957 pKa = 4.15WLRR960 pKa = 11.84QCWSLLGWFNMFWLVGMVSMVVLVLYY986 pKa = 8.75FCQRR990 pKa = 11.84RR991 pKa = 11.84ASPTHH996 pKa = 4.71IVKK999 pKa = 10.51YY1000 pKa = 10.08IKK1002 pKa = 10.63SSS1004 pKa = 3.08

Molecular weight: 110.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KKQ5|A0A0B5KKQ5_9VIRU Glycoprotein OS=Yichang Insect virus OX=1608144 GN=G PE=4 SV=1
MM1 pKa = 7.34TGVADD6 pKa = 3.83EE7 pKa = 4.64ARR9 pKa = 11.84AILEE13 pKa = 4.18SLSTEE18 pKa = 4.25TVFGGMNLDD27 pKa = 3.66VAEE30 pKa = 4.37MEE32 pKa = 4.48YY33 pKa = 10.65QGFSPDD39 pKa = 3.42LLIRR43 pKa = 11.84AMWEE47 pKa = 3.65LATKK51 pKa = 10.33KK52 pKa = 10.74GILADD57 pKa = 3.82SFKK60 pKa = 11.11KK61 pKa = 10.32DD62 pKa = 3.64LRR64 pKa = 11.84TMAVMGLMRR73 pKa = 11.84GSNIRR78 pKa = 11.84AIKK81 pKa = 10.34AKK83 pKa = 9.47STMEE87 pKa = 3.99AKK89 pKa = 10.44AQLTSWEE96 pKa = 4.21TTYY99 pKa = 11.45GLKK102 pKa = 10.24SGKK105 pKa = 7.53PTSSTTVTLVRR116 pKa = 11.84VSACLARR123 pKa = 11.84PMSLAMYY130 pKa = 9.18NGSLAISGAVPAEE143 pKa = 3.74AVVPGFPKK151 pKa = 10.69GMALSSFGSLIPGVDD166 pKa = 4.15TIPDD170 pKa = 3.7EE171 pKa = 4.56VCRR174 pKa = 11.84TLADD178 pKa = 2.91AFMYY182 pKa = 9.8HH183 pKa = 5.79QYY185 pKa = 11.38KK186 pKa = 9.29FDD188 pKa = 3.81KK189 pKa = 10.82VINSNTNRR197 pKa = 11.84EE198 pKa = 3.74TSIANIKK205 pKa = 10.15QYY207 pKa = 8.91TAIQMRR213 pKa = 11.84SNLYY217 pKa = 10.41DD218 pKa = 3.31SNARR222 pKa = 11.84VNHH225 pKa = 6.4CCTINILVKK234 pKa = 10.8DD235 pKa = 4.09GSTYY239 pKa = 10.92KK240 pKa = 10.75LSPSCSTALTTAAEE254 pKa = 4.42LFRR257 pKa = 11.84ALSS260 pKa = 3.55

Molecular weight: 28.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3353

260

2089

1117.7

125.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.875 ± 1.039

2.654 ± 1.435

5.637 ± 0.451

6.024 ± 0.922

3.639 ± 0.374

5.637 ± 0.407

2.058 ± 0.429

5.607 ± 0.2

5.577 ± 0.431

9.514 ± 0.392

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.668 ± 0.523

3.698 ± 0.225

4.384 ± 0.318

3.161 ± 0.242

5.786 ± 0.755

9.514 ± 0.45

6.203 ± 0.696

6.531 ± 0.216

1.64 ± 0.201

3.191 ± 0.083
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski