Amino acid dipepetide frequency for Pedobacter sp. RP-1-14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.394AlaAla: 6.394 ± 0.097
0.642AlaCys: 0.642 ± 0.024
4.437AlaAsp: 4.437 ± 0.056
4.734AlaGlu: 4.734 ± 0.059
3.501AlaPhe: 3.501 ± 0.043
5.778AlaGly: 5.778 ± 0.087
1.222AlaHis: 1.222 ± 0.03
5.453AlaIle: 5.453 ± 0.066
4.757AlaLys: 4.757 ± 0.068
6.864AlaLeu: 6.864 ± 0.072
1.761AlaMet: 1.761 ± 0.04
3.791AlaAsn: 3.791 ± 0.06
2.265AlaPro: 2.265 ± 0.045
2.747AlaGln: 2.747 ± 0.038
2.592AlaArg: 2.592 ± 0.043
4.589AlaSer: 4.589 ± 0.059
4.079AlaThr: 4.079 ± 0.062
4.933AlaVal: 4.933 ± 0.067
0.766AlaTrp: 0.766 ± 0.024
2.914AlaTyr: 2.914 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.488CysAla: 0.488 ± 0.019
0.126CysCys: 0.126 ± 0.01
0.365CysAsp: 0.365 ± 0.014
0.363CysGlu: 0.363 ± 0.014
0.399CysPhe: 0.399 ± 0.017
0.585CysGly: 0.585 ± 0.021
0.187CysHis: 0.187 ± 0.013
0.6CysIle: 0.6 ± 0.019
0.526CysLys: 0.526 ± 0.019
0.716CysLeu: 0.716 ± 0.022
0.185CysMet: 0.185 ± 0.01
0.35CysAsn: 0.35 ± 0.015
0.28CysPro: 0.28 ± 0.011
0.197CysGln: 0.197 ± 0.011
0.301CysArg: 0.301 ± 0.014
0.473CysSer: 0.473 ± 0.017
0.374CysThr: 0.374 ± 0.017
0.425CysVal: 0.425 ± 0.018
0.081CysTrp: 0.081 ± 0.007
0.293CysTyr: 0.293 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
3.847AspAla: 3.847 ± 0.055
0.365AspCys: 0.365 ± 0.016
2.521AspAsp: 2.521 ± 0.043
3.359AspGlu: 3.359 ± 0.048
3.158AspPhe: 3.158 ± 0.041
3.9AspGly: 3.9 ± 0.067
1.001AspHis: 1.001 ± 0.024
3.973AspIle: 3.973 ± 0.052
4.055AspLys: 4.055 ± 0.055
5.247AspLeu: 5.247 ± 0.058
1.226AspMet: 1.226 ± 0.027
2.741AspAsn: 2.741 ± 0.047
2.291AspPro: 2.291 ± 0.042
2.083AspGln: 2.083 ± 0.042
2.201AspArg: 2.201 ± 0.036
2.915AspSer: 2.915 ± 0.053
2.579AspThr: 2.579 ± 0.047
3.448AspVal: 3.448 ± 0.055
0.764AspTrp: 0.764 ± 0.023
2.508AspTyr: 2.508 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
4.224GluAla: 4.224 ± 0.071
0.307GluCys: 0.307 ± 0.015
2.838GluAsp: 2.838 ± 0.041
3.719GluGlu: 3.719 ± 0.055
2.469GluPhe: 2.469 ± 0.044
3.558GluGly: 3.558 ± 0.051
1.016GluHis: 1.016 ± 0.025
4.588GluIle: 4.588 ± 0.059
4.607GluLys: 4.607 ± 0.061
5.991GluLeu: 5.991 ± 0.067
1.484GluMet: 1.484 ± 0.032
3.267GluAsn: 3.267 ± 0.053
1.648GluPro: 1.648 ± 0.034
2.448GluGln: 2.448 ± 0.045
2.619GluArg: 2.619 ± 0.041
2.938GluSer: 2.938 ± 0.047
2.892GluThr: 2.892 ± 0.045
3.925GluVal: 3.925 ± 0.051
0.64GluTrp: 0.64 ± 0.021
2.071GluTyr: 2.071 ± 0.038
0.001GluXaa: 0.001 ± 0.001
Phe
3.286PheAla: 3.286 ± 0.047
0.446PheCys: 0.446 ± 0.017
2.905PheAsp: 2.905 ± 0.044
2.765PheGlu: 2.765 ± 0.046
2.297PhePhe: 2.297 ± 0.048
3.426PheGly: 3.426 ± 0.055
0.786PheHis: 0.786 ± 0.022
3.565PheIle: 3.565 ± 0.061
3.466PheLys: 3.466 ± 0.048
4.35PheLeu: 4.35 ± 0.06
1.132PheMet: 1.132 ± 0.025
3.037PheAsn: 3.037 ± 0.042
1.723PhePro: 1.723 ± 0.036
1.357PheGln: 1.357 ± 0.028
1.804PheArg: 1.804 ± 0.036
3.913PheSer: 3.913 ± 0.055
3.029PheThr: 3.029 ± 0.044
2.825PheVal: 2.825 ± 0.043
0.585PheTrp: 0.585 ± 0.022
2.052PheTyr: 2.052 ± 0.043
0.0PheXaa: 0.0 ± 0.0
Gly
4.817GlyAla: 4.817 ± 0.075
0.602GlyCys: 0.602 ± 0.023
3.499GlyAsp: 3.499 ± 0.057
3.411GlyGlu: 3.411 ± 0.052
3.654GlyPhe: 3.654 ± 0.053
4.945GlyGly: 4.945 ± 0.088
1.179GlyHis: 1.179 ± 0.03
5.516GlyIle: 5.516 ± 0.071
5.55GlyLys: 5.55 ± 0.068
6.61GlyLeu: 6.61 ± 0.068
1.788GlyMet: 1.788 ± 0.038
3.866GlyAsn: 3.866 ± 0.063
1.689GlyPro: 1.689 ± 0.034
2.31GlyGln: 2.31 ± 0.04
2.718GlyArg: 2.718 ± 0.043
4.593GlySer: 4.593 ± 0.067
4.249GlyThr: 4.249 ± 0.064
4.387GlyVal: 4.387 ± 0.061
0.94GlyTrp: 0.94 ± 0.026
3.194GlyTyr: 3.194 ± 0.052
0.001GlyXaa: 0.001 ± 0.001
His
1.169HisAla: 1.169 ± 0.03
0.181HisCys: 0.181 ± 0.011
0.85HisAsp: 0.85 ± 0.027
0.963HisGlu: 0.963 ± 0.026
1.036HisPhe: 1.036 ± 0.026
1.11HisGly: 1.11 ± 0.023
0.491HisHis: 0.491 ± 0.021
1.341HisIle: 1.341 ± 0.028
1.076HisLys: 1.076 ± 0.027
1.736HisLeu: 1.736 ± 0.036
0.377HisMet: 0.377 ± 0.017
0.916HisAsn: 0.916 ± 0.024
0.922HisPro: 0.922 ± 0.024
0.713HisGln: 0.713 ± 0.025
0.712HisArg: 0.712 ± 0.02
1.007HisSer: 1.007 ± 0.026
0.991HisThr: 0.991 ± 0.025
0.933HisVal: 0.933 ± 0.023
0.249HisTrp: 0.249 ± 0.013
0.785HisTyr: 0.785 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
6.039IleAla: 6.039 ± 0.077
0.656IleCys: 0.656 ± 0.023
4.278IleAsp: 4.278 ± 0.058
4.345IleGlu: 4.345 ± 0.062
3.162IlePhe: 3.162 ± 0.053
5.031IleGly: 5.031 ± 0.068
1.218IleHis: 1.218 ± 0.028
5.112IleIle: 5.112 ± 0.087
5.188IleLys: 5.188 ± 0.059
6.304IleLeu: 6.304 ± 0.068
1.407IleMet: 1.407 ± 0.031
4.311IleAsn: 4.311 ± 0.062
3.055IlePro: 3.055 ± 0.045
2.242IleGln: 2.242 ± 0.04
3.011IleArg: 3.011 ± 0.042
5.514IleSer: 5.514 ± 0.067
4.59IleThr: 4.59 ± 0.061
4.421IleVal: 4.421 ± 0.06
0.762IleTrp: 0.762 ± 0.023
2.699IleTyr: 2.699 ± 0.045
0.0IleXaa: 0.0 ± 0.0
Lys
5.276LysAla: 5.276 ± 0.073
0.296LysCys: 0.296 ± 0.014
4.26LysAsp: 4.26 ± 0.058
4.734LysGlu: 4.734 ± 0.065
2.743LysPhe: 2.743 ± 0.046
4.743LysGly: 4.743 ± 0.055
1.309LysHis: 1.309 ± 0.029
5.316LysIle: 5.316 ± 0.066
5.435LysLys: 5.435 ± 0.064
6.374LysLeu: 6.374 ± 0.062
1.922LysMet: 1.922 ± 0.036
4.159LysAsn: 4.159 ± 0.054
2.859LysPro: 2.859 ± 0.049
2.678LysGln: 2.678 ± 0.043
2.843LysArg: 2.843 ± 0.041
4.263LysSer: 4.263 ± 0.05
4.11LysThr: 4.11 ± 0.057
4.608LysVal: 4.608 ± 0.053
0.818LysTrp: 0.818 ± 0.025
2.939LysTyr: 2.939 ± 0.05
0.0LysXaa: 0.0 ± 0.0
Leu
6.777LeuAla: 6.777 ± 0.086
0.77LeuCys: 0.77 ± 0.021
4.974LeuAsp: 4.974 ± 0.055
4.98LeuGlu: 4.98 ± 0.064
4.623LeuPhe: 4.623 ± 0.073
5.867LeuGly: 5.867 ± 0.06
1.653LeuHis: 1.653 ± 0.037
6.846LeuIle: 6.846 ± 0.078
7.535LeuLys: 7.535 ± 0.076
9.376LeuLeu: 9.376 ± 0.103
2.249LeuMet: 2.249 ± 0.046
5.806LeuAsn: 5.806 ± 0.068
3.971LeuPro: 3.971 ± 0.053
3.475LeuGln: 3.475 ± 0.05
3.771LeuArg: 3.771 ± 0.057
7.353LeuSer: 7.353 ± 0.073
5.541LeuThr: 5.541 ± 0.074
5.514LeuVal: 5.514 ± 0.064
0.978LeuTrp: 0.978 ± 0.027
3.277LeuTyr: 3.277 ± 0.049
0.0LeuXaa: 0.0 ± 0.0
Met
1.822MetAla: 1.822 ± 0.042
0.124MetCys: 0.124 ± 0.008
1.221MetAsp: 1.221 ± 0.029
1.427MetGlu: 1.427 ± 0.033
0.881MetPhe: 0.881 ± 0.026
1.588MetGly: 1.588 ± 0.035
0.409MetHis: 0.409 ± 0.017
1.631MetIle: 1.631 ± 0.036
2.029MetLys: 2.029 ± 0.033
2.302MetLeu: 2.302 ± 0.043
0.65MetMet: 0.65 ± 0.023
1.356MetAsn: 1.356 ± 0.028
1.054MetPro: 1.054 ± 0.029
0.982MetGln: 0.982 ± 0.027
0.993MetArg: 0.993 ± 0.028
1.408MetSer: 1.408 ± 0.034
1.146MetThr: 1.146 ± 0.025
1.516MetVal: 1.516 ± 0.036
0.193MetTrp: 0.193 ± 0.011
0.685MetTyr: 0.685 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
4.102AsnAla: 4.102 ± 0.054
0.368AsnCys: 0.368 ± 0.016
2.896AsnAsp: 2.896 ± 0.046
3.013AsnGlu: 3.013 ± 0.046
2.718AsnPhe: 2.718 ± 0.045
4.22AsnGly: 4.22 ± 0.065
0.922AsnHis: 0.922 ± 0.025
4.222AsnIle: 4.222 ± 0.063
3.893AsnLys: 3.893 ± 0.048
5.189AsnLeu: 5.189 ± 0.069
1.276AsnMet: 1.276 ± 0.033
3.295AsnAsn: 3.295 ± 0.058
2.712AsnPro: 2.712 ± 0.046
1.965AsnGln: 1.965 ± 0.036
2.273AsnArg: 2.273 ± 0.034
3.479AsnSer: 3.479 ± 0.06
3.279AsnThr: 3.279 ± 0.056
3.332AsnVal: 3.332 ± 0.048
0.743AsnTrp: 0.743 ± 0.022
2.592AsnTyr: 2.592 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
3.148ProAla: 3.148 ± 0.047
0.218ProCys: 0.218 ± 0.013
2.597ProAsp: 2.597 ± 0.04
2.819ProGlu: 2.819 ± 0.043
1.835ProPhe: 1.835 ± 0.032
2.884ProGly: 2.884 ± 0.049
0.641ProHis: 0.641 ± 0.021
2.372ProIle: 2.372 ± 0.042
2.284ProLys: 2.284 ± 0.036
3.391ProLeu: 3.391 ± 0.049
0.799ProMet: 0.799 ± 0.022
1.946ProAsn: 1.946 ± 0.033
0.954ProPro: 0.954 ± 0.029
1.312ProGln: 1.312 ± 0.032
1.141ProArg: 1.141 ± 0.032
2.282ProSer: 2.282 ± 0.035
1.78ProThr: 1.78 ± 0.035
3.237ProVal: 3.237 ± 0.052
0.394ProTrp: 0.394 ± 0.015
1.512ProTyr: 1.512 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
2.457GlnAla: 2.457 ± 0.039
0.173GlnCys: 0.173 ± 0.011
1.662GlnAsp: 1.662 ± 0.029
2.105GlnGlu: 2.105 ± 0.04
1.642GlnPhe: 1.642 ± 0.029
2.13GlnGly: 2.13 ± 0.042
0.73GlnHis: 0.73 ± 0.022
2.498GlnIle: 2.498 ± 0.043
2.502GlnLys: 2.502 ± 0.04
3.862GlnLeu: 3.862 ± 0.055
0.884GlnMet: 0.884 ± 0.026
2.056GlnAsn: 2.056 ± 0.037
1.322GlnPro: 1.322 ± 0.034
1.904GlnGln: 1.904 ± 0.04
1.517GlnArg: 1.517 ± 0.034
2.21GlnSer: 2.21 ± 0.042
2.079GlnThr: 2.079 ± 0.039
2.303GlnVal: 2.303 ± 0.036
0.402GlnTrp: 0.402 ± 0.016
1.446GlnTyr: 1.446 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
2.459ArgAla: 2.459 ± 0.043
0.219ArgCys: 0.219 ± 0.012
2.011ArgAsp: 2.011 ± 0.042
2.251ArgGlu: 2.251 ± 0.038
2.214ArgPhe: 2.214 ± 0.037
2.313ArgGly: 2.313 ± 0.041
0.693ArgHis: 0.693 ± 0.023
3.219ArgIle: 3.219 ± 0.053
3.019ArgLys: 3.019 ± 0.047
3.922ArgLeu: 3.922 ± 0.053
1.101ArgMet: 1.101 ± 0.025
2.41ArgAsn: 2.41 ± 0.046
1.428ArgPro: 1.428 ± 0.03
1.455ArgGln: 1.455 ± 0.028
1.617ArgArg: 1.617 ± 0.033
2.458ArgSer: 2.458 ± 0.045
2.081ArgThr: 2.081 ± 0.037
2.373ArgVal: 2.373 ± 0.044
0.509ArgTrp: 0.509 ± 0.018
1.859ArgTyr: 1.859 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
4.938SerAla: 4.938 ± 0.056
0.557SerCys: 0.557 ± 0.021
3.261SerAsp: 3.261 ± 0.046
3.171SerGlu: 3.171 ± 0.041
3.728SerPhe: 3.728 ± 0.054
5.293SerGly: 5.293 ± 0.069
1.081SerHis: 1.081 ± 0.027
4.849SerIle: 4.849 ± 0.062
4.157SerLys: 4.157 ± 0.056
6.427SerLeu: 6.427 ± 0.068
1.46SerMet: 1.46 ± 0.032
3.321SerAsn: 3.321 ± 0.044
2.476SerPro: 2.476 ± 0.038
1.951SerGln: 1.951 ± 0.035
2.686SerArg: 2.686 ± 0.043
4.352SerSer: 4.352 ± 0.068
3.563SerThr: 3.563 ± 0.045
4.326SerVal: 4.326 ± 0.057
0.802SerTrp: 0.802 ± 0.026
2.809SerTyr: 2.809 ± 0.049
0.0SerXaa: 0.0 ± 0.0
Thr
4.669ThrAla: 4.669 ± 0.064
0.337ThrCys: 0.337 ± 0.015
3.426ThrAsp: 3.426 ± 0.049
3.142ThrGlu: 3.142 ± 0.055
2.674ThrPhe: 2.674 ± 0.044
4.768ThrGly: 4.768 ± 0.063
0.903ThrHis: 0.903 ± 0.026
4.144ThrIle: 4.144 ± 0.051
3.294ThrLys: 3.294 ± 0.044
5.419ThrLeu: 5.419 ± 0.061
1.057ThrMet: 1.057 ± 0.025
2.89ThrAsn: 2.89 ± 0.049
2.409ThrPro: 2.409 ± 0.05
1.798ThrGln: 1.798 ± 0.034
1.955ThrArg: 1.955 ± 0.034
3.533ThrSer: 3.533 ± 0.055
3.406ThrThr: 3.406 ± 0.057
3.856ThrVal: 3.856 ± 0.057
0.644ThrTrp: 0.644 ± 0.023
2.323ThrTyr: 2.323 ± 0.042
0.001ThrXaa: 0.001 ± 0.001
Val
4.683ValAla: 4.683 ± 0.063
0.523ValCys: 0.523 ± 0.019
3.345ValAsp: 3.345 ± 0.052
3.265ValGlu: 3.265 ± 0.051
3.18ValPhe: 3.18 ± 0.041
3.719ValGly: 3.719 ± 0.062
1.022ValHis: 1.022 ± 0.027
4.749ValIle: 4.749 ± 0.063
4.704ValLys: 4.704 ± 0.053
6.159ValLeu: 6.159 ± 0.071
1.534ValMet: 1.534 ± 0.033
3.807ValAsn: 3.807 ± 0.056
2.494ValPro: 2.494 ± 0.044
2.089ValGln: 2.089 ± 0.036
2.471ValArg: 2.471 ± 0.04
4.571ValSer: 4.571 ± 0.057
3.717ValThr: 3.717 ± 0.058
4.336ValVal: 4.336 ± 0.065
0.683ValTrp: 0.683 ± 0.024
2.554ValTyr: 2.554 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
0.766TrpAla: 0.766 ± 0.025
0.107TrpCys: 0.107 ± 0.008
0.643TrpAsp: 0.643 ± 0.02
0.659TrpGlu: 0.659 ± 0.02
0.555TrpPhe: 0.555 ± 0.024
0.832TrpGly: 0.832 ± 0.024
0.245TrpHis: 0.245 ± 0.013
0.789TrpIle: 0.789 ± 0.022
0.85TrpLys: 0.85 ± 0.028
1.106TrpLeu: 1.106 ± 0.029
0.363TrpMet: 0.363 ± 0.016
0.685TrpAsn: 0.685 ± 0.023
0.379TrpPro: 0.379 ± 0.019
0.471TrpGln: 0.471 ± 0.018
0.495TrpArg: 0.495 ± 0.019
0.663TrpSer: 0.663 ± 0.021
0.639TrpThr: 0.639 ± 0.022
0.694TrpVal: 0.694 ± 0.022
0.189TrpTrp: 0.189 ± 0.011
0.504TrpTyr: 0.504 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.858TyrAla: 2.858 ± 0.049
0.295TyrCys: 0.295 ± 0.012
2.194TyrAsp: 2.194 ± 0.044
2.027TyrGlu: 2.027 ± 0.038
2.28TyrPhe: 2.28 ± 0.044
2.829TyrGly: 2.829 ± 0.05
0.851TyrHis: 0.851 ± 0.025
2.561TyrIle: 2.561 ± 0.048
2.758TyrLys: 2.758 ± 0.047
3.992TyrLeu: 3.992 ± 0.058
0.76TyrMet: 0.76 ± 0.021
2.48TyrAsn: 2.48 ± 0.046
1.614TyrPro: 1.614 ± 0.029
1.702TyrGln: 1.702 ± 0.041
1.894TyrArg: 1.894 ± 0.038
2.692TyrSer: 2.692 ± 0.044
2.525TyrThr: 2.525 ± 0.049
2.213TyrVal: 2.213 ± 0.037
0.491TyrTrp: 0.491 ± 0.017
1.801TyrTyr: 1.801 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.001XaaLys: 0.001 ± 0.001
0.0XaaLeu: 0.0 ± 0.0
0.001XaaMet: 0.001 ± 0.001
0.0XaaAsn: 0.0 ± 0.0
0.001XaaPro: 0.001 ± 0.001
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4198 proteins (1552746 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski