Rice ragged stunt virus (isolate Thailand) (RRSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Oryzavirus; Rice ragged stunt virus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>sp|O92604|RDRP_RRSVT RNA-directed RNA polymerase OS=Rice ragged stunt virus (isolate Thailand) OX=649603 PE=3 SV=1
MM1 pKa = 7.14QLFIVKK7 pKa = 10.07LDD9 pKa = 3.78SRR11 pKa = 11.84SSASNEE17 pKa = 3.56ILVLDD22 pKa = 3.64INEE25 pKa = 3.77LRR27 pKa = 11.84KK28 pKa = 9.93YY29 pKa = 9.86VSKK32 pKa = 10.46HH33 pKa = 3.85QLSFRR38 pKa = 11.84GAQVHH43 pKa = 5.18EE44 pKa = 4.62TNSEE48 pKa = 3.79HH49 pKa = 7.4SVRR52 pKa = 11.84NWEE55 pKa = 4.46DD56 pKa = 3.47DD57 pKa = 3.95NITMFFHH64 pKa = 6.79TSNVFPSEE72 pKa = 3.73KK73 pKa = 9.89SVRR76 pKa = 11.84HH77 pKa = 5.49ALFSRR82 pKa = 11.84NSVHH86 pKa = 6.25VCGPLNIGAFRR97 pKa = 11.84DD98 pKa = 3.74SLRR101 pKa = 11.84VLQSPQFPIHH111 pKa = 6.62TYY113 pKa = 11.0GDD115 pKa = 4.8DD116 pKa = 3.04ITVFDD121 pKa = 4.42KK122 pKa = 11.05LRR124 pKa = 11.84EE125 pKa = 4.03FVVVPNSLAVVDD137 pKa = 4.55MDD139 pKa = 3.85TSAWEE144 pKa = 4.13RR145 pKa = 11.84YY146 pKa = 7.3TYY148 pKa = 11.23SLFTDD153 pKa = 3.73EE154 pKa = 5.54PGTITLADD162 pKa = 3.97EE163 pKa = 4.6TPPTISPTGRR173 pKa = 11.84DD174 pKa = 3.13SVSPVARR181 pKa = 11.84FSPSISISSIAIGEE195 pKa = 4.21VGSASKK201 pKa = 10.41RR202 pKa = 11.84RR203 pKa = 11.84QRR205 pKa = 11.84LVKK208 pKa = 9.66QAKK211 pKa = 10.08SSDD214 pKa = 3.36KK215 pKa = 10.99TPIPEE220 pKa = 3.4IRR222 pKa = 11.84LNKK225 pKa = 9.53KK226 pKa = 8.93DD227 pKa = 3.54TVFAEE232 pKa = 4.43TGTEE236 pKa = 3.84QASPKK241 pKa = 9.34TEE243 pKa = 3.45IVAATVHH250 pKa = 6.68KK251 pKa = 10.61IPVNHH256 pKa = 7.02AEE258 pKa = 3.6ADD260 pKa = 3.5KK261 pKa = 11.04RR262 pKa = 11.84VVVLKK267 pKa = 10.97GRR269 pKa = 11.84TKK271 pKa = 10.95DD272 pKa = 2.94KK273 pKa = 10.02AQEE276 pKa = 4.01VRR278 pKa = 11.84EE279 pKa = 4.34VTSLARR285 pKa = 11.84DD286 pKa = 3.69TTGVEE291 pKa = 4.11HH292 pKa = 7.43EE293 pKa = 4.75LMQSVGDD300 pKa = 4.34DD301 pKa = 3.42SVQVSGEE308 pKa = 3.7LSGFGDD314 pKa = 3.68EE315 pKa = 4.43SFEE318 pKa = 4.09VSKK321 pKa = 10.21TDD323 pKa = 3.55VVGVEE328 pKa = 4.01NKK330 pKa = 9.59QLALDD335 pKa = 4.21RR336 pKa = 11.84VPALEE341 pKa = 4.13PCGEE345 pKa = 4.03VSEE348 pKa = 5.44DD349 pKa = 3.64EE350 pKa = 4.32EE351 pKa = 4.47LVSEE355 pKa = 4.34GMYY358 pKa = 10.59AVPLDD363 pKa = 3.76LDD365 pKa = 4.05IYY367 pKa = 11.12AEE369 pKa = 4.39KK370 pKa = 10.43CPSPTPPNSPKK381 pKa = 10.02KK382 pKa = 10.08RR383 pKa = 11.84EE384 pKa = 4.16KK385 pKa = 10.61ADD387 pKa = 3.7HH388 pKa = 6.26EE389 pKa = 4.92PIDD392 pKa = 3.84ISSAMMKK399 pKa = 10.26LRR401 pKa = 11.84LIAAAFQKK409 pKa = 10.85EE410 pKa = 3.74MMAYY414 pKa = 10.37DD415 pKa = 5.36KK416 pKa = 10.55IDD418 pKa = 4.19CLSSDD423 pKa = 3.75DD424 pKa = 4.17PAYY427 pKa = 11.21LLAQFCLRR435 pKa = 11.84NLNGVFVPEE444 pKa = 3.98YY445 pKa = 10.5VEE447 pKa = 4.07EE448 pKa = 4.33HH449 pKa = 7.01SSDD452 pKa = 3.79EE453 pKa = 4.3EE454 pKa = 4.26NADD457 pKa = 5.2DD458 pKa = 6.32PNQGWNGTPVHH469 pKa = 6.66GSRR472 pKa = 11.84EE473 pKa = 4.05IFEE476 pKa = 4.64KK477 pKa = 10.78LSEE480 pKa = 4.15SRR482 pKa = 11.84LASMEE487 pKa = 4.08MISNVKK493 pKa = 9.33PIRR496 pKa = 11.84WNSKK500 pKa = 6.77KK501 pKa = 6.88TTYY504 pKa = 11.04AVTIEE509 pKa = 4.32RR510 pKa = 11.84GNPNLIVTQSLTRR523 pKa = 11.84GQSSQFRR530 pKa = 11.84KK531 pKa = 9.69VIRR534 pKa = 11.84AFGCSTDD541 pKa = 3.43YY542 pKa = 11.1CLEE545 pKa = 4.07RR546 pKa = 11.84LASRR550 pKa = 11.84DD551 pKa = 3.04YY552 pKa = 11.46DD553 pKa = 4.71AILMLMSSLSGACHH567 pKa = 5.56ATKK570 pKa = 10.2SYY572 pKa = 11.11CYY574 pKa = 9.73LQIVAYY580 pKa = 9.86GSFAAPEE587 pKa = 4.35CKK589 pKa = 10.2EE590 pKa = 3.81LDD592 pKa = 3.46

Molecular weight:
65.77 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>sp|O92605|VP4B_RRSVT Uncharacterized protein VP4b OS=Rice ragged stunt virus (isolate Thailand) OX=649603 PE=4 SV=1
MM1 pKa = 7.02TLLVITEE8 pKa = 4.01QTIHH12 pKa = 6.36SLCLDD17 pKa = 3.55HH18 pKa = 7.54GEE20 pKa = 4.25TNQIIAEE27 pKa = 4.31IKK29 pKa = 9.86QLEE32 pKa = 4.3KK33 pKa = 10.93PEE35 pKa = 4.73LLFSYY40 pKa = 9.02ITDD43 pKa = 4.04AEE45 pKa = 4.16PLATGEE51 pKa = 4.36VFVGPDD57 pKa = 2.66ICGNCITHH65 pKa = 6.44TFRR68 pKa = 11.84VPDD71 pKa = 3.99YY72 pKa = 10.03VAKK75 pKa = 10.26PPPYY79 pKa = 9.45DD80 pKa = 3.36SKK82 pKa = 10.99RR83 pKa = 11.84VYY85 pKa = 10.38YY86 pKa = 9.4PYY88 pKa = 10.84SYY90 pKa = 10.3TCLGFDD96 pKa = 3.53SHH98 pKa = 7.41PYY100 pKa = 10.45DD101 pKa = 3.97YY102 pKa = 11.48LLTLDD107 pKa = 4.07TKK109 pKa = 11.13SIFQAIRR116 pKa = 11.84KK117 pKa = 7.15ITLTRR122 pKa = 11.84KK123 pKa = 10.12LSIATQSDD131 pKa = 3.94LDD133 pKa = 4.55LIILKK138 pKa = 10.56KK139 pKa = 10.18LTTQSNCQSRR149 pKa = 11.84VSSIWRR155 pKa = 11.84QCVAACLAFEE165 pKa = 4.74PQIQNNNSTQSPNLNNLVKK184 pKa = 10.76RR185 pKa = 11.84MFATLLKK192 pKa = 10.39PIGRR196 pKa = 11.84LPFYY200 pKa = 9.91EE201 pKa = 3.86RR202 pKa = 11.84RR203 pKa = 11.84RR204 pKa = 11.84NFVWEE209 pKa = 4.41EE210 pKa = 3.87EE211 pKa = 4.7VSCPTILPLLLYY223 pKa = 10.33SIQNLVTQFCAGVINRR239 pKa = 11.84MEE241 pKa = 4.56MILAFQYY248 pKa = 11.37YY249 pKa = 9.84LDD251 pKa = 4.76CGVTAYY257 pKa = 10.4QDD259 pKa = 3.28EE260 pKa = 4.47KK261 pKa = 11.48LRR263 pKa = 11.84LQKK266 pKa = 10.69LIRR269 pKa = 11.84NWLRR273 pKa = 11.84EE274 pKa = 3.92GLAKK278 pKa = 10.38FSKK281 pKa = 10.1IAMPNWTVSGVISCTTVKK299 pKa = 10.41PIMITTHH306 pKa = 5.56EE307 pKa = 3.92ARR309 pKa = 11.84EE310 pKa = 4.07IKK312 pKa = 10.02SARR315 pKa = 11.84EE316 pKa = 3.71ARR318 pKa = 11.84EE319 pKa = 3.76ASVPTYY325 pKa = 10.15LLQYY329 pKa = 9.57INHH332 pKa = 7.75AIHH335 pKa = 6.75KK336 pKa = 9.57AISPYY341 pKa = 8.13QTHH344 pKa = 6.14QYY346 pKa = 8.1WQATRR351 pKa = 11.84VFANDD356 pKa = 3.11GTYY359 pKa = 9.8FASVAALTLNKK370 pKa = 9.9AVRR373 pKa = 11.84PRR375 pKa = 11.84IEE377 pKa = 4.2EE378 pKa = 4.17TTVKK382 pKa = 10.46YY383 pKa = 10.5PNSRR387 pKa = 11.84YY388 pKa = 8.42LTMNKK393 pKa = 7.92EE394 pKa = 4.39HH395 pKa = 6.97IAVSLPNEE403 pKa = 3.81TDD405 pKa = 3.37TNFGLACQFVKK416 pKa = 10.67QFIPRR421 pKa = 11.84LVTRR425 pKa = 11.84LKK427 pKa = 9.28NTNFQEE433 pKa = 3.96EE434 pKa = 4.96FIAFLTSSSSGKK446 pKa = 10.18DD447 pKa = 3.36FEE449 pKa = 4.94DD450 pKa = 3.58AAIATMTRR458 pKa = 11.84TIQRR462 pKa = 11.84VAKK465 pKa = 9.71KK466 pKa = 10.15RR467 pKa = 11.84IVAAGLEE474 pKa = 4.27SRR476 pKa = 11.84SYY478 pKa = 11.42LNQQFIADD486 pKa = 4.01EE487 pKa = 4.2CLAAAKK493 pKa = 10.26LVGRR497 pKa = 11.84TQIGRR502 pKa = 11.84RR503 pKa = 11.84QRR505 pKa = 11.84AIAGVNNTRR514 pKa = 11.84SLLGFPPMLMLKK526 pKa = 10.36ALLDD530 pKa = 3.74MTGTTSSGKK539 pKa = 10.33QMGNYY544 pKa = 9.7LDD546 pKa = 5.07LLIPLGLSPYY556 pKa = 10.86ANVIFNSADD565 pKa = 3.09VDD567 pKa = 3.79AMDD570 pKa = 5.09ASVQASVQQIMWHH583 pKa = 5.17FVVYY587 pKa = 10.41IALQLEE593 pKa = 4.33RR594 pKa = 11.84TDD596 pKa = 3.92YY597 pKa = 11.03FAFTSGDD604 pKa = 3.39EE605 pKa = 4.44VIYY608 pKa = 10.48EE609 pKa = 4.06LVEE612 pKa = 4.5GKK614 pKa = 10.48DD615 pKa = 3.56SVATSIHH622 pKa = 5.57MSGLARR628 pKa = 11.84SCLRR632 pKa = 11.84AMHH635 pKa = 7.19LLQPQNCVLNDD646 pKa = 4.5DD647 pKa = 4.0IVGEE651 pKa = 4.15LVTRR655 pKa = 11.84EE656 pKa = 3.9PTFPSGQPFTTVHH669 pKa = 5.35HH670 pKa = 6.65TFTLSNAIMGGTLRR684 pKa = 11.84VTNLTNQPSTLLNLTVQGDD703 pKa = 3.81DD704 pKa = 3.43TRR706 pKa = 11.84TINYY710 pKa = 8.31GPKK713 pKa = 10.27GCIEE717 pKa = 4.22KK718 pKa = 10.9CIDD721 pKa = 3.71DD722 pKa = 3.63QSSFVSDD729 pKa = 2.79WGFKK733 pKa = 10.17VSNEE737 pKa = 4.25TSSHH741 pKa = 4.49TSEE744 pKa = 4.36YY745 pKa = 9.32LQQRR749 pKa = 11.84VSCGTFVGYY758 pKa = 9.19PDD760 pKa = 3.86RR761 pKa = 11.84VSLFAAEE768 pKa = 4.19RR769 pKa = 11.84PKK771 pKa = 10.64EE772 pKa = 3.99GKK774 pKa = 8.01TMKK777 pKa = 10.47EE778 pKa = 3.74KK779 pKa = 10.37MSEE782 pKa = 3.5IWSLVTDD789 pKa = 4.21LGCRR793 pKa = 11.84SRR795 pKa = 11.84DD796 pKa = 3.44PQRR799 pKa = 11.84LVRR802 pKa = 11.84LMYY805 pKa = 10.63AIGVACCCRR814 pKa = 11.84LTIRR818 pKa = 11.84TEE820 pKa = 3.79RR821 pKa = 11.84LVAEE825 pKa = 4.23EE826 pKa = 5.04FIASEE831 pKa = 4.26VGRR834 pKa = 11.84ACCAEE839 pKa = 3.67ILVPKK844 pKa = 9.99VAARR848 pKa = 11.84ADD850 pKa = 3.8RR851 pKa = 11.84GVMLRR856 pKa = 11.84YY857 pKa = 9.53HH858 pKa = 6.26YY859 pKa = 9.71PISALWLEE867 pKa = 4.86EE868 pKa = 4.42GGQLPPLATKK878 pKa = 10.48RR879 pKa = 11.84RR880 pKa = 11.84DD881 pKa = 3.77GTWTCFPSYY890 pKa = 10.26YY891 pKa = 9.92FQRR894 pKa = 11.84GDD896 pKa = 3.83SNRR899 pKa = 11.84YY900 pKa = 7.69WYY902 pKa = 9.64WDD904 pKa = 3.32VSLTEE909 pKa = 4.49EE910 pKa = 3.79KK911 pKa = 10.19WITTVEE917 pKa = 4.08MRR919 pKa = 11.84SDD921 pKa = 3.15WDD923 pKa = 3.8SNNAIEE929 pKa = 6.01LIDD932 pKa = 4.81DD933 pKa = 5.42EE934 pKa = 4.71ILEE937 pKa = 4.7AYY939 pKa = 7.12ATRR942 pKa = 11.84YY943 pKa = 9.99ALAALGLNKK952 pKa = 8.77TRR954 pKa = 11.84QMEE957 pKa = 4.13RR958 pKa = 11.84LRR960 pKa = 11.84LSEE963 pKa = 4.03EE964 pKa = 3.77STIFDD969 pKa = 3.78VEE971 pKa = 4.18QLASGLNAYY980 pKa = 10.13RR981 pKa = 11.84NLSKK985 pKa = 10.49IAISHH990 pKa = 6.39RR991 pKa = 11.84ASEE994 pKa = 4.18RR995 pKa = 11.84LKK997 pKa = 10.71NAGVEE1002 pKa = 4.23LPDD1005 pKa = 3.3SVLYY1009 pKa = 10.58SRR1011 pKa = 11.84HH1012 pKa = 4.42TQSRR1016 pKa = 11.84IQDD1019 pKa = 4.42AILKK1023 pKa = 10.0AQMTEE1028 pKa = 3.82KK1029 pKa = 11.03EE1030 pKa = 4.45EE1031 pKa = 4.52IEE1033 pKa = 3.78MSLYY1037 pKa = 10.02FFKK1040 pKa = 10.97DD1041 pKa = 2.78KK1042 pKa = 11.2GYY1044 pKa = 10.5LRR1046 pKa = 11.84SLRR1049 pKa = 11.84EE1050 pKa = 3.93LVKK1053 pKa = 10.94SKK1055 pKa = 10.91SGDD1058 pKa = 2.91LAMIHH1063 pKa = 5.69RR1064 pKa = 11.84CEE1066 pKa = 3.71WGEE1069 pKa = 3.72RR1070 pKa = 11.84VTILEE1075 pKa = 3.91SSIFNVVSTTPLSMEE1090 pKa = 4.43VYY1092 pKa = 9.29PGSIMSFILCYY1103 pKa = 9.93TGLKK1107 pKa = 9.26GAHH1110 pKa = 6.28SGQLAGLVGLLQGKK1124 pKa = 9.66YY1125 pKa = 9.71GGGKK1129 pKa = 9.16ISEE1132 pKa = 4.41LQFSIAKK1139 pKa = 9.78KK1140 pKa = 9.97IYY1142 pKa = 8.38LTKK1145 pKa = 10.26PYY1147 pKa = 10.14LLNEE1151 pKa = 4.56FIIACGLGGEE1161 pKa = 4.98AEE1163 pKa = 4.29LALKK1167 pKa = 10.2NALHH1171 pKa = 6.28AFEE1174 pKa = 4.48MLRR1177 pKa = 11.84GVEE1180 pKa = 4.52FSTVHH1185 pKa = 5.8TPRR1188 pKa = 11.84QFFFGVDD1195 pKa = 3.17SGVTLGNHH1203 pKa = 6.61LDD1205 pKa = 3.9YY1206 pKa = 11.31PNGFPGTRR1214 pKa = 11.84IEE1216 pKa = 4.29LAAHH1220 pKa = 7.18LLLAMNFLSSNAQSCTGRR1238 pKa = 11.84SIRR1241 pKa = 11.84VRR1243 pKa = 11.84VPRR1246 pKa = 11.84GMWSRR1251 pKa = 11.84CTNFF1255 pKa = 3.21

Molecular weight:
141.38 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

8760

297

1255

730.0

81.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.112 ± 0.224

1.804 ± 0.243

5.331 ± 0.216

5.982 ± 0.365

4.178 ± 0.194

5.833 ± 0.207

1.929 ± 0.132

5.925 ± 0.264

4.852 ± 0.221

9.555 ± 0.323

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.13

4.178 ± 0.211

4.578 ± 0.242

3.63 ± 0.249

5.993 ± 0.289

8.573 ± 0.366

6.119 ± 0.254

6.986 ± 0.388

1.119 ± 0.128

3.904 ± 0.164

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski