Rice ragged stunt virus (isolate Thailand) (RRSV)
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|O92604|RDRP_RRSVT RNA-directed RNA polymerase OS=Rice ragged stunt virus (isolate Thailand) OX=649603 PE=3 SV=1
MM1 pKa = 7.14 QLFIVKK7 pKa = 10.07 LDD9 pKa = 3.78 SRR11 pKa = 11.84 SSASNEE17 pKa = 3.56 ILVLDD22 pKa = 3.64 INEE25 pKa = 3.77 LRR27 pKa = 11.84 KK28 pKa = 9.93 YY29 pKa = 9.86 VSKK32 pKa = 10.46 HH33 pKa = 3.85 QLSFRR38 pKa = 11.84 GAQVHH43 pKa = 5.18 EE44 pKa = 4.62 TNSEE48 pKa = 3.79 HH49 pKa = 7.4 SVRR52 pKa = 11.84 NWEE55 pKa = 4.46 DD56 pKa = 3.47 DD57 pKa = 3.95 NITMFFHH64 pKa = 6.79 TSNVFPSEE72 pKa = 3.73 KK73 pKa = 9.89 SVRR76 pKa = 11.84 HH77 pKa = 5.49 ALFSRR82 pKa = 11.84 NSVHH86 pKa = 6.25 VCGPLNIGAFRR97 pKa = 11.84 DD98 pKa = 3.74 SLRR101 pKa = 11.84 VLQSPQFPIHH111 pKa = 6.62 TYY113 pKa = 11.0 GDD115 pKa = 4.8 DD116 pKa = 3.04 ITVFDD121 pKa = 4.42 KK122 pKa = 11.05 LRR124 pKa = 11.84 EE125 pKa = 4.03 FVVVPNSLAVVDD137 pKa = 4.55 MDD139 pKa = 3.85 TSAWEE144 pKa = 4.13 RR145 pKa = 11.84 YY146 pKa = 7.3 TYY148 pKa = 11.23 SLFTDD153 pKa = 3.73 EE154 pKa = 5.54 PGTITLADD162 pKa = 3.97 EE163 pKa = 4.6 TPPTISPTGRR173 pKa = 11.84 DD174 pKa = 3.13 SVSPVARR181 pKa = 11.84 FSPSISISSIAIGEE195 pKa = 4.21 VGSASKK201 pKa = 10.41 RR202 pKa = 11.84 RR203 pKa = 11.84 QRR205 pKa = 11.84 LVKK208 pKa = 9.66 QAKK211 pKa = 10.08 SSDD214 pKa = 3.36 KK215 pKa = 10.99 TPIPEE220 pKa = 3.4 IRR222 pKa = 11.84 LNKK225 pKa = 9.53 KK226 pKa = 8.93 DD227 pKa = 3.54 TVFAEE232 pKa = 4.43 TGTEE236 pKa = 3.84 QASPKK241 pKa = 9.34 TEE243 pKa = 3.45 IVAATVHH250 pKa = 6.68 KK251 pKa = 10.61 IPVNHH256 pKa = 7.02 AEE258 pKa = 3.6 ADD260 pKa = 3.5 KK261 pKa = 11.04 RR262 pKa = 11.84 VVVLKK267 pKa = 10.97 GRR269 pKa = 11.84 TKK271 pKa = 10.95 DD272 pKa = 2.94 KK273 pKa = 10.02 AQEE276 pKa = 4.01 VRR278 pKa = 11.84 EE279 pKa = 4.34 VTSLARR285 pKa = 11.84 DD286 pKa = 3.69 TTGVEE291 pKa = 4.11 HH292 pKa = 7.43 EE293 pKa = 4.75 LMQSVGDD300 pKa = 4.34 DD301 pKa = 3.42 SVQVSGEE308 pKa = 3.7 LSGFGDD314 pKa = 3.68 EE315 pKa = 4.43 SFEE318 pKa = 4.09 VSKK321 pKa = 10.21 TDD323 pKa = 3.55 VVGVEE328 pKa = 4.01 NKK330 pKa = 9.59 QLALDD335 pKa = 4.21 RR336 pKa = 11.84 VPALEE341 pKa = 4.13 PCGEE345 pKa = 4.03 VSEE348 pKa = 5.44 DD349 pKa = 3.64 EE350 pKa = 4.32 EE351 pKa = 4.47 LVSEE355 pKa = 4.34 GMYY358 pKa = 10.59 AVPLDD363 pKa = 3.76 LDD365 pKa = 4.05 IYY367 pKa = 11.12 AEE369 pKa = 4.39 KK370 pKa = 10.43 CPSPTPPNSPKK381 pKa = 10.02 KK382 pKa = 10.08 RR383 pKa = 11.84 EE384 pKa = 4.16 KK385 pKa = 10.61 ADD387 pKa = 3.7 HH388 pKa = 6.26 EE389 pKa = 4.92 PIDD392 pKa = 3.84 ISSAMMKK399 pKa = 10.26 LRR401 pKa = 11.84 LIAAAFQKK409 pKa = 10.85 EE410 pKa = 3.74 MMAYY414 pKa = 10.37 DD415 pKa = 5.36 KK416 pKa = 10.55 IDD418 pKa = 4.19 CLSSDD423 pKa = 3.75 DD424 pKa = 4.17 PAYY427 pKa = 11.21 LLAQFCLRR435 pKa = 11.84 NLNGVFVPEE444 pKa = 3.98 YY445 pKa = 10.5 VEE447 pKa = 4.07 EE448 pKa = 4.33 HH449 pKa = 7.01 SSDD452 pKa = 3.79 EE453 pKa = 4.3 EE454 pKa = 4.26 NADD457 pKa = 5.2 DD458 pKa = 6.32 PNQGWNGTPVHH469 pKa = 6.66 GSRR472 pKa = 11.84 EE473 pKa = 4.05 IFEE476 pKa = 4.64 KK477 pKa = 10.78 LSEE480 pKa = 4.15 SRR482 pKa = 11.84 LASMEE487 pKa = 4.08 MISNVKK493 pKa = 9.33 PIRR496 pKa = 11.84 WNSKK500 pKa = 6.77 KK501 pKa = 6.88 TTYY504 pKa = 11.04 AVTIEE509 pKa = 4.32 RR510 pKa = 11.84 GNPNLIVTQSLTRR523 pKa = 11.84 GQSSQFRR530 pKa = 11.84 KK531 pKa = 9.69 VIRR534 pKa = 11.84 AFGCSTDD541 pKa = 3.43 YY542 pKa = 11.1 CLEE545 pKa = 4.07 RR546 pKa = 11.84 LASRR550 pKa = 11.84 DD551 pKa = 3.04 YY552 pKa = 11.46 DD553 pKa = 4.71 AILMLMSSLSGACHH567 pKa = 5.56 ATKK570 pKa = 10.2 SYY572 pKa = 11.11 CYY574 pKa = 9.73 LQIVAYY580 pKa = 9.86 GSFAAPEE587 pKa = 4.35 CKK589 pKa = 10.2 EE590 pKa = 3.81 LDD592 pKa = 3.46
Molecular weight: 65.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.151
IPC2_protein 5.169
IPC_protein 5.143
Toseland 5.13
ProMoST 5.258
Dawson 5.156
Bjellqvist 5.283
Wikipedia 5.041
Rodwell 5.08
Grimsley 5.067
Solomon 5.156
Lehninger 5.118
Nozaki 5.296
DTASelect 5.448
Thurlkill 5.143
EMBOSS 5.105
Sillero 5.372
Patrickios 4.164
IPC_peptide 5.169
IPC2_peptide 5.372
IPC2.peptide.svr19 5.315
Protein with the highest isoelectric point:
>sp|O92605|VP4B_RRSVT Uncharacterized protein VP4b OS=Rice ragged stunt virus (isolate Thailand) OX=649603 PE=4 SV=1
MM1 pKa = 7.02 TLLVITEE8 pKa = 4.01 QTIHH12 pKa = 6.36 SLCLDD17 pKa = 3.55 HH18 pKa = 7.54 GEE20 pKa = 4.25 TNQIIAEE27 pKa = 4.31 IKK29 pKa = 9.86 QLEE32 pKa = 4.3 KK33 pKa = 10.93 PEE35 pKa = 4.73 LLFSYY40 pKa = 9.02 ITDD43 pKa = 4.04 AEE45 pKa = 4.16 PLATGEE51 pKa = 4.36 VFVGPDD57 pKa = 2.66 ICGNCITHH65 pKa = 6.44 TFRR68 pKa = 11.84 VPDD71 pKa = 3.99 YY72 pKa = 10.03 VAKK75 pKa = 10.26 PPPYY79 pKa = 9.45 DD80 pKa = 3.36 SKK82 pKa = 10.99 RR83 pKa = 11.84 VYY85 pKa = 10.38 YY86 pKa = 9.4 PYY88 pKa = 10.84 SYY90 pKa = 10.3 TCLGFDD96 pKa = 3.53 SHH98 pKa = 7.41 PYY100 pKa = 10.45 DD101 pKa = 3.97 YY102 pKa = 11.48 LLTLDD107 pKa = 4.07 TKK109 pKa = 11.13 SIFQAIRR116 pKa = 11.84 KK117 pKa = 7.15 ITLTRR122 pKa = 11.84 KK123 pKa = 10.12 LSIATQSDD131 pKa = 3.94 LDD133 pKa = 4.55 LIILKK138 pKa = 10.56 KK139 pKa = 10.18 LTTQSNCQSRR149 pKa = 11.84 VSSIWRR155 pKa = 11.84 QCVAACLAFEE165 pKa = 4.74 PQIQNNNSTQSPNLNNLVKK184 pKa = 10.76 RR185 pKa = 11.84 MFATLLKK192 pKa = 10.39 PIGRR196 pKa = 11.84 LPFYY200 pKa = 9.91 EE201 pKa = 3.86 RR202 pKa = 11.84 RR203 pKa = 11.84 RR204 pKa = 11.84 NFVWEE209 pKa = 4.41 EE210 pKa = 3.87 EE211 pKa = 4.7 VSCPTILPLLLYY223 pKa = 10.33 SIQNLVTQFCAGVINRR239 pKa = 11.84 MEE241 pKa = 4.56 MILAFQYY248 pKa = 11.37 YY249 pKa = 9.84 LDD251 pKa = 4.76 CGVTAYY257 pKa = 10.4 QDD259 pKa = 3.28 EE260 pKa = 4.47 KK261 pKa = 11.48 LRR263 pKa = 11.84 LQKK266 pKa = 10.69 LIRR269 pKa = 11.84 NWLRR273 pKa = 11.84 EE274 pKa = 3.92 GLAKK278 pKa = 10.38 FSKK281 pKa = 10.1 IAMPNWTVSGVISCTTVKK299 pKa = 10.41 PIMITTHH306 pKa = 5.56 EE307 pKa = 3.92 ARR309 pKa = 11.84 EE310 pKa = 4.07 IKK312 pKa = 10.02 SARR315 pKa = 11.84 EE316 pKa = 3.71 ARR318 pKa = 11.84 EE319 pKa = 3.76 ASVPTYY325 pKa = 10.15 LLQYY329 pKa = 9.57 INHH332 pKa = 7.75 AIHH335 pKa = 6.75 KK336 pKa = 9.57 AISPYY341 pKa = 8.13 QTHH344 pKa = 6.14 QYY346 pKa = 8.1 WQATRR351 pKa = 11.84 VFANDD356 pKa = 3.11 GTYY359 pKa = 9.8 FASVAALTLNKK370 pKa = 9.9 AVRR373 pKa = 11.84 PRR375 pKa = 11.84 IEE377 pKa = 4.2 EE378 pKa = 4.17 TTVKK382 pKa = 10.46 YY383 pKa = 10.5 PNSRR387 pKa = 11.84 YY388 pKa = 8.42 LTMNKK393 pKa = 7.92 EE394 pKa = 4.39 HH395 pKa = 6.97 IAVSLPNEE403 pKa = 3.81 TDD405 pKa = 3.37 TNFGLACQFVKK416 pKa = 10.67 QFIPRR421 pKa = 11.84 LVTRR425 pKa = 11.84 LKK427 pKa = 9.28 NTNFQEE433 pKa = 3.96 EE434 pKa = 4.96 FIAFLTSSSSGKK446 pKa = 10.18 DD447 pKa = 3.36 FEE449 pKa = 4.94 DD450 pKa = 3.58 AAIATMTRR458 pKa = 11.84 TIQRR462 pKa = 11.84 VAKK465 pKa = 9.71 KK466 pKa = 10.15 RR467 pKa = 11.84 IVAAGLEE474 pKa = 4.27 SRR476 pKa = 11.84 SYY478 pKa = 11.42 LNQQFIADD486 pKa = 4.01 EE487 pKa = 4.2 CLAAAKK493 pKa = 10.26 LVGRR497 pKa = 11.84 TQIGRR502 pKa = 11.84 RR503 pKa = 11.84 QRR505 pKa = 11.84 AIAGVNNTRR514 pKa = 11.84 SLLGFPPMLMLKK526 pKa = 10.36 ALLDD530 pKa = 3.74 MTGTTSSGKK539 pKa = 10.33 QMGNYY544 pKa = 9.7 LDD546 pKa = 5.07 LLIPLGLSPYY556 pKa = 10.86 ANVIFNSADD565 pKa = 3.09 VDD567 pKa = 3.79 AMDD570 pKa = 5.09 ASVQASVQQIMWHH583 pKa = 5.17 FVVYY587 pKa = 10.41 IALQLEE593 pKa = 4.33 RR594 pKa = 11.84 TDD596 pKa = 3.92 YY597 pKa = 11.03 FAFTSGDD604 pKa = 3.39 EE605 pKa = 4.44 VIYY608 pKa = 10.48 EE609 pKa = 4.06 LVEE612 pKa = 4.5 GKK614 pKa = 10.48 DD615 pKa = 3.56 SVATSIHH622 pKa = 5.57 MSGLARR628 pKa = 11.84 SCLRR632 pKa = 11.84 AMHH635 pKa = 7.19 LLQPQNCVLNDD646 pKa = 4.5 DD647 pKa = 4.0 IVGEE651 pKa = 4.15 LVTRR655 pKa = 11.84 EE656 pKa = 3.9 PTFPSGQPFTTVHH669 pKa = 5.35 HH670 pKa = 6.65 TFTLSNAIMGGTLRR684 pKa = 11.84 VTNLTNQPSTLLNLTVQGDD703 pKa = 3.81 DD704 pKa = 3.43 TRR706 pKa = 11.84 TINYY710 pKa = 8.31 GPKK713 pKa = 10.27 GCIEE717 pKa = 4.22 KK718 pKa = 10.9 CIDD721 pKa = 3.71 DD722 pKa = 3.63 QSSFVSDD729 pKa = 2.79 WGFKK733 pKa = 10.17 VSNEE737 pKa = 4.25 TSSHH741 pKa = 4.49 TSEE744 pKa = 4.36 YY745 pKa = 9.32 LQQRR749 pKa = 11.84 VSCGTFVGYY758 pKa = 9.19 PDD760 pKa = 3.86 RR761 pKa = 11.84 VSLFAAEE768 pKa = 4.19 RR769 pKa = 11.84 PKK771 pKa = 10.64 EE772 pKa = 3.99 GKK774 pKa = 8.01 TMKK777 pKa = 10.47 EE778 pKa = 3.74 KK779 pKa = 10.37 MSEE782 pKa = 3.5 IWSLVTDD789 pKa = 4.21 LGCRR793 pKa = 11.84 SRR795 pKa = 11.84 DD796 pKa = 3.44 PQRR799 pKa = 11.84 LVRR802 pKa = 11.84 LMYY805 pKa = 10.63 AIGVACCCRR814 pKa = 11.84 LTIRR818 pKa = 11.84 TEE820 pKa = 3.79 RR821 pKa = 11.84 LVAEE825 pKa = 4.23 EE826 pKa = 5.04 FIASEE831 pKa = 4.26 VGRR834 pKa = 11.84 ACCAEE839 pKa = 3.67 ILVPKK844 pKa = 9.99 VAARR848 pKa = 11.84 ADD850 pKa = 3.8 RR851 pKa = 11.84 GVMLRR856 pKa = 11.84 YY857 pKa = 9.53 HH858 pKa = 6.26 YY859 pKa = 9.71 PISALWLEE867 pKa = 4.86 EE868 pKa = 4.42 GGQLPPLATKK878 pKa = 10.48 RR879 pKa = 11.84 RR880 pKa = 11.84 DD881 pKa = 3.77 GTWTCFPSYY890 pKa = 10.26 YY891 pKa = 9.92 FQRR894 pKa = 11.84 GDD896 pKa = 3.83 SNRR899 pKa = 11.84 YY900 pKa = 7.69 WYY902 pKa = 9.64 WDD904 pKa = 3.32 VSLTEE909 pKa = 4.49 EE910 pKa = 3.79 KK911 pKa = 10.19 WITTVEE917 pKa = 4.08 MRR919 pKa = 11.84 SDD921 pKa = 3.15 WDD923 pKa = 3.8 SNNAIEE929 pKa = 6.01 LIDD932 pKa = 4.81 DD933 pKa = 5.42 EE934 pKa = 4.71 ILEE937 pKa = 4.7 AYY939 pKa = 7.12 ATRR942 pKa = 11.84 YY943 pKa = 9.99 ALAALGLNKK952 pKa = 8.77 TRR954 pKa = 11.84 QMEE957 pKa = 4.13 RR958 pKa = 11.84 LRR960 pKa = 11.84 LSEE963 pKa = 4.03 EE964 pKa = 3.77 STIFDD969 pKa = 3.78 VEE971 pKa = 4.18 QLASGLNAYY980 pKa = 10.13 RR981 pKa = 11.84 NLSKK985 pKa = 10.49 IAISHH990 pKa = 6.39 RR991 pKa = 11.84 ASEE994 pKa = 4.18 RR995 pKa = 11.84 LKK997 pKa = 10.71 NAGVEE1002 pKa = 4.23 LPDD1005 pKa = 3.3 SVLYY1009 pKa = 10.58 SRR1011 pKa = 11.84 HH1012 pKa = 4.42 TQSRR1016 pKa = 11.84 IQDD1019 pKa = 4.42 AILKK1023 pKa = 10.0 AQMTEE1028 pKa = 3.82 KK1029 pKa = 11.03 EE1030 pKa = 4.45 EE1031 pKa = 4.52 IEE1033 pKa = 3.78 MSLYY1037 pKa = 10.02 FFKK1040 pKa = 10.97 DD1041 pKa = 2.78 KK1042 pKa = 11.2 GYY1044 pKa = 10.5 LRR1046 pKa = 11.84 SLRR1049 pKa = 11.84 EE1050 pKa = 3.93 LVKK1053 pKa = 10.94 SKK1055 pKa = 10.91 SGDD1058 pKa = 2.91 LAMIHH1063 pKa = 5.69 RR1064 pKa = 11.84 CEE1066 pKa = 3.71 WGEE1069 pKa = 3.72 RR1070 pKa = 11.84 VTILEE1075 pKa = 3.91 SSIFNVVSTTPLSMEE1090 pKa = 4.43 VYY1092 pKa = 9.29 PGSIMSFILCYY1103 pKa = 9.93 TGLKK1107 pKa = 9.26 GAHH1110 pKa = 6.28 SGQLAGLVGLLQGKK1124 pKa = 9.66 YY1125 pKa = 9.71 GGGKK1129 pKa = 9.16 ISEE1132 pKa = 4.41 LQFSIAKK1139 pKa = 9.78 KK1140 pKa = 9.97 IYY1142 pKa = 8.38 LTKK1145 pKa = 10.26 PYY1147 pKa = 10.14 LLNEE1151 pKa = 4.56 FIIACGLGGEE1161 pKa = 4.98 AEE1163 pKa = 4.29 LALKK1167 pKa = 10.2 NALHH1171 pKa = 6.28 AFEE1174 pKa = 4.48 MLRR1177 pKa = 11.84 GVEE1180 pKa = 4.52 FSTVHH1185 pKa = 5.8 TPRR1188 pKa = 11.84 QFFFGVDD1195 pKa = 3.17 SGVTLGNHH1203 pKa = 6.61 LDD1205 pKa = 3.9 YY1206 pKa = 11.31 PNGFPGTRR1214 pKa = 11.84 IEE1216 pKa = 4.29 LAAHH1220 pKa = 7.18 LLLAMNFLSSNAQSCTGRR1238 pKa = 11.84 SIRR1241 pKa = 11.84 VRR1243 pKa = 11.84 VPRR1246 pKa = 11.84 GMWSRR1251 pKa = 11.84 CTNFF1255 pKa = 3.21
Molecular weight: 141.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.069
IPC2_protein 7.41
IPC_protein 7.19
Toseland 6.854
ProMoST 7.805
Dawson 7.892
Bjellqvist 8.375
Wikipedia 7.805
Rodwell 7.922
Grimsley 6.912
Solomon 7.922
Lehninger 7.936
Nozaki 8.565
DTASelect 8.053
Thurlkill 8.097
EMBOSS 8.112
Sillero 8.448
Patrickios 4.113
IPC_peptide 7.922
IPC2_peptide 7.483
IPC2.peptide.svr19 7.393
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
8760
297
1255
730.0
81.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.112 ± 0.224
1.804 ± 0.243
5.331 ± 0.216
5.982 ± 0.365
4.178 ± 0.194
5.833 ± 0.207
1.929 ± 0.132
5.925 ± 0.264
4.852 ± 0.221
9.555 ± 0.323
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.42 ± 0.13
4.178 ± 0.211
4.578 ± 0.242
3.63 ± 0.249
5.993 ± 0.289
8.573 ± 0.366
6.119 ± 0.254
6.986 ± 0.388
1.119 ± 0.128
3.904 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here