Mycobacterium phage Squirty

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Squirtyvirus; Mycobacterium virus Squirty

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088F7S9|A0A088F7S9_9CAUD Uncharacterized protein OS=Mycobacterium phage Squirty OX=1527512 GN=96 PE=4 SV=1
MM1 pKa = 6.84TTMDD5 pKa = 3.6TRR7 pKa = 11.84VTLAISLLLTGEE19 pKa = 3.98PRR21 pKa = 11.84LVDD24 pKa = 3.65EE25 pKa = 4.97ACEE28 pKa = 4.2LMEE31 pKa = 4.55EE32 pKa = 4.67LCDD35 pKa = 3.76EE36 pKa = 4.58LPAPRR41 pKa = 11.84ACNPIRR47 pKa = 11.84YY48 pKa = 8.5PLDD51 pKa = 3.94GDD53 pKa = 4.02TPP55 pKa = 3.97

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088FBI9|A0A088FBI9_9CAUD Uncharacterized protein OS=Mycobacterium phage Squirty OX=1527512 GN=44 PE=4 SV=1
MM1 pKa = 7.6GFTTRR6 pKa = 11.84DD7 pKa = 3.11MYY9 pKa = 11.5AEE11 pKa = 4.96CDD13 pKa = 3.09LALFVRR19 pKa = 11.84ALRR22 pKa = 11.84RR23 pKa = 11.84SGLTYY28 pKa = 10.28EE29 pKa = 4.41ALADD33 pKa = 3.75EE34 pKa = 4.97SARR37 pKa = 11.84EE38 pKa = 3.81LRR40 pKa = 11.84KK41 pKa = 9.32IARR44 pKa = 11.84AEE46 pKa = 3.76KK47 pKa = 9.84RR48 pKa = 11.84KK49 pKa = 10.02RR50 pKa = 11.84RR51 pKa = 11.84GDD53 pKa = 4.18GIPTTISKK61 pKa = 10.06QLIGQLATGRR71 pKa = 11.84AKK73 pKa = 9.57STHH76 pKa = 5.53EE77 pKa = 3.71LRR79 pKa = 11.84GIAIEE84 pKa = 4.09RR85 pKa = 11.84ALGVGDD91 pKa = 3.85GDD93 pKa = 3.43IFVPRR98 pKa = 11.84VVRR101 pKa = 11.84GVRR104 pKa = 11.84TEE106 pKa = 3.52QRR108 pKa = 11.84TAA110 pKa = 3.0

Molecular weight: 12.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

19151

30

1201

187.8

20.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.263 ± 0.581

1.29 ± 0.155

6.94 ± 0.208

5.853 ± 0.305

2.726 ± 0.163

8.135 ± 0.536

2.329 ± 0.215

4.423 ± 0.167

3.243 ± 0.193

7.78 ± 0.196

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.151 ± 0.097

2.929 ± 0.147

5.937 ± 0.21

3.587 ± 0.16

7.41 ± 0.39

5.707 ± 0.202

6.271 ± 0.264

7.227 ± 0.257

2.235 ± 0.134

2.564 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski