Afipia sp. Root123D2
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4397 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
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Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0Q6ZHN4|A0A0Q6ZHN4_9BRAD Acetyl-CoA acetyltransferase OS=Afipia sp. Root123D2 OX=1736436 GN=ASC80_08660 PE=3 SV=1
MM1 pKa = 7.36 NAPVLLAQLSGSVQTPSPEE20 pKa = 4.02 PKK22 pKa = 8.87 TLKK25 pKa = 10.07 IEE27 pKa = 4.24 KK28 pKa = 8.86 PANGQALNFHH38 pKa = 7.04 LDD40 pKa = 2.98 GNTRR44 pKa = 11.84 LDD46 pKa = 3.65 LSDD49 pKa = 3.38 IASEE53 pKa = 3.91 KK54 pKa = 9.9 LTFVKK59 pKa = 10.56 VGEE62 pKa = 4.07 KK63 pKa = 10.65 LIVLFDD69 pKa = 3.69 NQSTVTVDD77 pKa = 3.44 PVFDD81 pKa = 3.85 SATGNPLTDD90 pKa = 2.86 VGFQVDD96 pKa = 3.68 GDD98 pKa = 3.75 RR99 pKa = 11.84 VLNGEE104 pKa = 3.9 QFAALFPISTDD115 pKa = 3.52 QSILPAAGNSGPAGGADD132 pKa = 3.37 FHH134 pKa = 6.93 DD135 pKa = 4.52 AQVGALGPGGTPLALLGDD153 pKa = 4.26 EE154 pKa = 4.65 TSGDD158 pKa = 3.63 QQFGTTEE165 pKa = 3.81 TGFAGITFGTATAGNVDD182 pKa = 5.16 DD183 pKa = 5.55 EE184 pKa = 4.59 GLSDD188 pKa = 4.25 GLVGGPNDD196 pKa = 3.7 APGNATSVTGSLNIDD211 pKa = 4.03 FGSVGVSGASLAFLAGQPGLAGLTSGGEE239 pKa = 4.34 TIHH242 pKa = 7.54 LLISNPAGGTPTIVGFVGNDD262 pKa = 3.3 PNVAGSHH269 pKa = 4.83 VFEE272 pKa = 5.68 ISLDD276 pKa = 3.92 LNTSTGEE283 pKa = 3.9 YY284 pKa = 9.87 TFTLLRR290 pKa = 11.84 PLDD293 pKa = 3.59 HH294 pKa = 7.1 SGANTEE300 pKa = 4.21 DD301 pKa = 3.77 TLNLTISYY309 pKa = 9.58 SASNASGTVTSAFGVNVNDD328 pKa = 5.78 DD329 pKa = 3.97 SPEE332 pKa = 4.06 TNPNGFDD339 pKa = 4.32 QPLTTQGSEE348 pKa = 3.82 GSEE351 pKa = 4.02 GSEE354 pKa = 3.92 GGLIVPHH361 pKa = 7.34 ADD363 pKa = 4.04 LKK365 pKa = 11.54 DD366 pKa = 3.73 PDD368 pKa = 4.2 GGTSVRR374 pKa = 11.84 GGTLGILWGADD385 pKa = 2.6 HH386 pKa = 7.18 FNAHH390 pKa = 6.03 EE391 pKa = 4.38 NGGISATTGQTGDD404 pKa = 3.2 RR405 pKa = 11.84 SLVFTNATVEE415 pKa = 4.03 LSNGEE420 pKa = 4.02 GSLTNSLFSHH430 pKa = 6.76 GEE432 pKa = 3.75 PIQFQLSNDD441 pKa = 3.52 GTRR444 pKa = 11.84 LIGFTGGGDD453 pKa = 3.35 EE454 pKa = 5.44 GPNEE458 pKa = 4.06 GNIVFTVDD466 pKa = 5.79 LRR468 pKa = 11.84 DD469 pKa = 3.7 TDD471 pKa = 3.24 NGGYY475 pKa = 10.39 VFTQYY480 pKa = 10.92 QEE482 pKa = 4.11 LDD484 pKa = 3.42 HH485 pKa = 7.16 VGTSGEE491 pKa = 3.98 QQQINLASLDD501 pKa = 3.71 LTFHH505 pKa = 5.58 YY506 pKa = 8.58 TATDD510 pKa = 3.34 SDD512 pKa = 4.16 GDD514 pKa = 4.16 SVAGSFNVTVSDD526 pKa = 4.07 DD527 pKa = 3.6 APTISVEE534 pKa = 4.28 GIEE537 pKa = 4.16 NGQVAEE543 pKa = 4.7 DD544 pKa = 4.1 RR545 pKa = 11.84 FDD547 pKa = 3.53 NGNPGDD553 pKa = 4.0 TYY555 pKa = 11.3 GANQGTEE562 pKa = 3.86 SLTFNGFLGVSWGADD577 pKa = 3.43 DD578 pKa = 4.96 NNSGSLANRR587 pKa = 11.84 SLAFNGVSEE596 pKa = 4.25 GGEE599 pKa = 4.06 VKK601 pKa = 10.7 AQDD604 pKa = 3.56 GTSITSHH611 pKa = 6.57 DD612 pKa = 3.88 QAVHH616 pKa = 4.48 YY617 pKa = 10.77 HH618 pKa = 6.73 LMTVGDD624 pKa = 3.74 QSVLIGYY631 pKa = 7.27 TGAAPADD638 pKa = 4.08 DD639 pKa = 3.52 QSNWVFKK646 pKa = 11.43 VEE648 pKa = 5.13 LKK650 pKa = 9.81 DD651 pKa = 3.58 TSDD654 pKa = 3.22 TGSYY658 pKa = 9.43 TFTLLQPIDD667 pKa = 3.7 QPVIDD672 pKa = 5.0 SEE674 pKa = 4.81 DD675 pKa = 3.56 DD676 pKa = 3.4 VTIDD680 pKa = 4.24 FGFTATDD687 pKa = 3.48 ADD689 pKa = 3.89 GDD691 pKa = 3.9 IANGSFSVAINDD703 pKa = 4.23 DD704 pKa = 3.9 APASSGAVEE713 pKa = 4.22 ARR715 pKa = 11.84 TVDD718 pKa = 3.23 EE719 pKa = 5.01 GDD721 pKa = 4.17 IKK723 pKa = 9.77 TLWSTGSSPNDD734 pKa = 3.77 GAGDD738 pKa = 3.89 GSDD741 pKa = 3.18 TGGPLASFLSGLFGFALGGPAYY763 pKa = 10.71 LSGTVATTVAFGADD777 pKa = 3.47 GAHH780 pKa = 6.79 AGGGFGFAADD790 pKa = 3.69 AAATMEE796 pKa = 4.31 ALGLTSKK803 pKa = 10.82 GQTVHH808 pKa = 6.16 YY809 pKa = 11.0 AMIGNTLVGYY819 pKa = 10.07 VDD821 pKa = 3.66 QPIFDD826 pKa = 4.26 RR827 pKa = 11.84 PVLALTLKK835 pKa = 10.64 SDD837 pKa = 3.16 GSYY840 pKa = 10.66 EE841 pKa = 4.0 FRR843 pKa = 11.84 QFDD846 pKa = 3.63 QLDD849 pKa = 3.76 HH850 pKa = 6.74 VSGGEE855 pKa = 3.97 HH856 pKa = 6.37 NLALTTSGGSIDD868 pKa = 5.46 AIDD871 pKa = 4.41 FGALITATDD880 pKa = 3.75 GDD882 pKa = 4.2 GDD884 pKa = 4.61 HH885 pKa = 7.17 ISLAGKK891 pKa = 7.89 FTIEE895 pKa = 4.11 IQDD898 pKa = 4.06 DD899 pKa = 4.31 VPVATINASGAVTIDD914 pKa = 3.36 EE915 pKa = 4.6 TGGNSNGDD923 pKa = 3.53 NTSSNSVRR931 pKa = 11.84 HH932 pKa = 5.86 LFDD935 pKa = 3.3 EE936 pKa = 4.51 VGNRR940 pKa = 11.84 GSDD943 pKa = 3.33 PDD945 pKa = 3.53 MSGPVYY951 pKa = 10.85 ARR953 pKa = 11.84 DD954 pKa = 3.63 NLVSTTTTTGADD966 pKa = 3.45 DD967 pKa = 3.91 TGAKK971 pKa = 10.02 ALSLHH976 pKa = 6.54 IDD978 pKa = 3.57 ALDD981 pKa = 3.9 SGLTTTDD988 pKa = 2.37 GHH990 pKa = 6.8 SIYY993 pKa = 10.17 LYY995 pKa = 10.07 QVSDD999 pKa = 3.64 TLVVGRR1005 pKa = 11.84 VDD1007 pKa = 3.89 GEE1009 pKa = 4.08 TGEE1012 pKa = 4.28 AAFAVTIDD1020 pKa = 3.13 QSGRR1024 pKa = 11.84 VSVAQYY1030 pKa = 11.32 LSLHH1034 pKa = 6.61 HH1035 pKa = 6.99 NDD1037 pKa = 3.9 DD1038 pKa = 3.72 TTSNDD1043 pKa = 3.57 SVDD1046 pKa = 3.74 LTGKK1050 pKa = 10.21 LSAVVTVTDD1059 pKa = 3.59 HH1060 pKa = 7.48 DD1061 pKa = 4.72 GDD1063 pKa = 3.87 IVTQATDD1070 pKa = 2.92 IGDD1073 pKa = 4.12 NIQFRR1078 pKa = 11.84 DD1079 pKa = 4.22 DD1080 pKa = 3.62 GPKK1083 pKa = 10.44 LVGTVTSGLTLNEE1096 pKa = 4.68 DD1097 pKa = 4.53 DD1098 pKa = 5.77 LPTGNDD1104 pKa = 3.16 STKK1107 pKa = 10.68 EE1108 pKa = 3.83 ALSTNGNLNIDD1119 pKa = 4.18 LGSDD1123 pKa = 3.65 GGNVVLSAEE1132 pKa = 4.23 GASWNSATHH1141 pKa = 6.42 TLTASDD1147 pKa = 4.26 GAWTVTLNSNGTYY1160 pKa = 10.25 TFTLLDD1166 pKa = 3.56 NTLLHH1171 pKa = 6.82 GPANDD1176 pKa = 3.9 GTNTLSVPVTYY1187 pKa = 9.16 TATDD1191 pKa = 3.41 GDD1193 pKa = 4.12 GDD1195 pKa = 4.2 VIAGSFTVKK1204 pKa = 10.18 IVDD1207 pKa = 4.05 DD1208 pKa = 4.79 APTASLSKK1216 pKa = 10.31 MVVQEE1221 pKa = 3.94 TADD1224 pKa = 3.56 ANGSFHH1230 pKa = 6.8 EE1231 pKa = 4.57 QTTTDD1236 pKa = 3.73 HH1237 pKa = 6.5 FHH1239 pKa = 7.2 FSPGADD1245 pKa = 3.37 GAKK1248 pKa = 8.77 VTAIHH1253 pKa = 6.75 YY1254 pKa = 9.72 DD1255 pKa = 3.41 FGTNIADD1262 pKa = 3.69 GDD1264 pKa = 4.03 ATPFTSLALTSGGEE1278 pKa = 3.96 QVIVTEE1284 pKa = 4.52 STDD1287 pKa = 3.28 HH1288 pKa = 6.04 LTLTGALADD1297 pKa = 3.88 GTSVFVITVTNPATGEE1313 pKa = 3.99 YY1314 pKa = 10.15 SFTQSAPIDD1323 pKa = 3.85 HH1324 pKa = 7.59 PDD1326 pKa = 3.44 VNEE1329 pKa = 4.82 SGAQDD1334 pKa = 4.23 PLRR1337 pKa = 11.84 MRR1339 pKa = 11.84 ISFTVTDD1346 pKa = 4.11 SDD1348 pKa = 4.17 GDD1350 pKa = 3.75 TATNHH1355 pKa = 5.62 IQLDD1359 pKa = 3.97 INDD1362 pKa = 4.89 DD1363 pKa = 3.83 APTAANFNAAMFEE1376 pKa = 4.28 KK1377 pKa = 9.84 QTVTLDD1383 pKa = 3.76 LAGHH1387 pKa = 5.73 YY1388 pKa = 10.38 AYY1390 pKa = 10.36 GADD1393 pKa = 3.95 GANDD1397 pKa = 3.68 ADD1399 pKa = 4.02 GTVLGTAYY1407 pKa = 10.73 VSGLPDD1413 pKa = 3.66 GVVLGLPSITLNPDD1427 pKa = 2.38 GHH1429 pKa = 6.31 TVTIQPGWALDD1440 pKa = 3.83 GLSQDD1445 pKa = 3.73 QQATLHH1451 pKa = 6.38 IPFTVTDD1458 pKa = 3.98 GDD1460 pKa = 4.01 GDD1462 pKa = 4.19 TVTRR1466 pKa = 11.84 EE1467 pKa = 3.62 ILVTVTGTNDD1477 pKa = 3.24 GLGTSEE1483 pKa = 4.11 GTPDD1487 pKa = 3.52 GGTMTEE1493 pKa = 4.13 YY1494 pKa = 11.3 AEE1496 pKa = 4.43 TDD1498 pKa = 3.25 LHH1500 pKa = 7.37 AGSTDD1505 pKa = 3.02 DD1506 pKa = 4.05 RR1507 pKa = 11.84 TIFDD1511 pKa = 4.48 APPGYY1516 pKa = 10.31 GGYY1519 pKa = 10.41 NGGTFHH1525 pKa = 7.45 LYY1527 pKa = 10.76 DD1528 pKa = 4.84 PDD1530 pKa = 3.57 QSDD1533 pKa = 3.17 THH1535 pKa = 6.55 TIVSAEE1541 pKa = 4.18 PYY1543 pKa = 9.25 PAGSNYY1549 pKa = 9.24 IGHH1552 pKa = 5.63 VTASVTQEE1560 pKa = 4.19 TIGDD1564 pKa = 3.97 GAGVVSWQYY1573 pKa = 11.31 HH1574 pKa = 4.07 VTDD1577 pKa = 3.98 AALNPLSAGEE1587 pKa = 4.45 HH1588 pKa = 4.62 KK1589 pKa = 9.79 TEE1591 pKa = 4.07 TFRR1594 pKa = 11.84 ITVQDD1599 pKa = 3.33 NHH1601 pKa = 7.59 GEE1603 pKa = 4.1 TTYY1606 pKa = 11.28 RR1607 pKa = 11.84 DD1608 pKa = 3.5 VTIEE1612 pKa = 3.88 LVGTNDD1618 pKa = 3.3 SPIITVGNGDD1628 pKa = 3.23 SAYY1631 pKa = 11.2 RR1632 pKa = 11.84 MIDD1635 pKa = 3.16 EE1636 pKa = 4.53 TNAGLSAQGTLTVTDD1651 pKa = 3.8 VDD1653 pKa = 4.01 TLDD1656 pKa = 3.73 TVHH1659 pKa = 6.47 ATVLSVSAGGSGIEE1673 pKa = 4.09 HH1674 pKa = 7.32 LYY1676 pKa = 11.39 DD1677 pKa = 3.56 NATLLSMLTVAPDD1690 pKa = 3.52 PVLDD1694 pKa = 4.06 SNGARR1699 pKa = 11.84 TDD1701 pKa = 3.39 ALTWTFDD1708 pKa = 3.41 SHH1710 pKa = 8.34 GEE1712 pKa = 3.98 AFNFLPNGWEE1722 pKa = 3.99 SVLNYY1727 pKa = 8.93 TIQVSDD1733 pKa = 3.28 GHH1735 pKa = 7.59 GGFDD1739 pKa = 3.5 TQVVQIKK1746 pKa = 10.01 LHH1748 pKa = 6.17 GSNDD1752 pKa = 3.23 APIITSSALGSVTEE1766 pKa = 4.92 AGDD1769 pKa = 3.79 LPPVDD1774 pKa = 3.77 EE1775 pKa = 5.47 AGLGGTLTVAAALLTLNVTDD1795 pKa = 5.58 ALATLEE1801 pKa = 4.05 NHH1803 pKa = 6.96 PSDD1806 pKa = 3.63 LHH1808 pKa = 6.42 NVLTTVQAEE1817 pKa = 4.17 QGGNLATAIATVWQHH1832 pKa = 6.75 LNDD1835 pKa = 4.32 AYY1837 pKa = 10.13 VTAAPTDD1844 pKa = 3.57 VPAINEE1850 pKa = 3.87 AFTRR1854 pKa = 11.84 LGVEE1858 pKa = 3.72 YY1859 pKa = 8.82 ATYY1862 pKa = 10.4 LKK1864 pKa = 10.68 NGGMPLVDD1872 pKa = 4.47 VIGKK1876 pKa = 7.48 YY1877 pKa = 7.99 TADD1880 pKa = 3.92 ANLSGAPDD1888 pKa = 3.91 RR1889 pKa = 11.84 LQSLHH1894 pKa = 7.51 DD1895 pKa = 4.0 NLLGNLGADD1904 pKa = 3.76 ALTQRR1909 pKa = 11.84 YY1910 pKa = 6.87 GTSALHH1916 pKa = 6.57 DD1917 pKa = 3.7 QLEE1920 pKa = 4.06 ALITGVDD1927 pKa = 3.72 PNLLDD1932 pKa = 3.57 RR1933 pKa = 11.84 PYY1935 pKa = 11.35 ASGGEE1940 pKa = 4.36 GSSGVHH1946 pKa = 7.13 DD1947 pKa = 3.8 AHH1949 pKa = 7.52 AWDD1952 pKa = 4.81 AEE1954 pKa = 4.36 HH1955 pKa = 7.31 GYY1957 pKa = 8.4 ATHH1960 pKa = 7.18 VSGTLTATDD1969 pKa = 4.44 ADD1971 pKa = 4.22 SSSLTWTAGTQSGTYY1986 pKa = 8.89 GTFTVDD1992 pKa = 5.16 ASGHH1996 pKa = 3.96 WTYY1999 pKa = 11.62 VLDD2002 pKa = 3.97 NDD2004 pKa = 5.28 RR2005 pKa = 11.84 PDD2007 pKa = 3.85 TEE2009 pKa = 4.46 ALAAGTHH2016 pKa = 4.68 GTEE2019 pKa = 4.19 TFTVTVTDD2027 pKa = 3.69 NSNTSTASQVTITIDD2042 pKa = 3.55 GTNDD2046 pKa = 2.95 APEE2049 pKa = 4.21 ITSSVLTAPISTQLHH2064 pKa = 5.33 NVQQADD2070 pKa = 3.42 ATHH2073 pKa = 6.17 TFEE2076 pKa = 4.41 PTANLDD2082 pKa = 3.72 TEE2084 pKa = 4.08 IAAALGIPAGPSPDD2098 pKa = 3.21 TAADD2102 pKa = 3.27 MKK2104 pKa = 11.22 VIVTNVQTGLGGGATFADD2122 pKa = 5.66 AIAQVWDD2129 pKa = 3.91 YY2130 pKa = 11.74 LDD2132 pKa = 4.81 EE2133 pKa = 4.54 NYY2135 pKa = 10.28 QYY2137 pKa = 11.81 YY2138 pKa = 7.61 NTPVNHH2144 pKa = 6.76 ASVLLGLVYY2153 pKa = 10.71 AEE2155 pKa = 4.66 YY2156 pKa = 9.97 IQNGGQPLLDD2166 pKa = 4.41 TIAKK2170 pKa = 9.77 FNADD2174 pKa = 4.11 TNDD2177 pKa = 3.98 GIADD2181 pKa = 4.37 RR2182 pKa = 11.84 SQSLHH2187 pKa = 7.39 DD2188 pKa = 4.14 NLLGNLDD2195 pKa = 4.61 LPSIDD2200 pKa = 5.16 DD2201 pKa = 3.87 KK2202 pKa = 11.71 FGANPALRR2210 pKa = 11.84 DD2211 pKa = 3.76 YY2212 pKa = 11.17 LLHH2215 pKa = 6.06 QVEE2218 pKa = 4.56 DD2219 pKa = 3.86 AGLSGRR2225 pKa = 11.84 PIYY2228 pKa = 10.81 SGEE2231 pKa = 4.0 QADD2234 pKa = 4.54 NPTAAHH2240 pKa = 6.08 TWDD2243 pKa = 3.5 QTHH2246 pKa = 6.6 GLLAVASGQLTAIDD2260 pKa = 3.27 VDD2262 pKa = 4.37 ANHH2265 pKa = 6.71 TLTWSVAGGASPYY2278 pKa = 10.23 GTMTVDD2284 pKa = 3.62 QTGAWHH2290 pKa = 6.22 FTADD2294 pKa = 3.66 DD2295 pKa = 3.84 NAATRR2300 pKa = 11.84 ALAAGEE2306 pKa = 4.04 EE2307 pKa = 4.57 SAPQTFTIQVADD2319 pKa = 3.48 EE2320 pKa = 4.69 HH2321 pKa = 6.53 GAIDD2325 pKa = 4.66 TIPVSIRR2332 pKa = 11.84 ITGTNDD2338 pKa = 2.82 APVITQTHH2346 pKa = 4.57 EE2347 pKa = 4.14 TVVNAGFEE2355 pKa = 4.33 ADD2357 pKa = 3.05 TSYY2360 pKa = 11.63 NQFGSEE2366 pKa = 3.9 GQYY2369 pKa = 11.03 TNGSPSGWSVTGPSYY2384 pKa = 11.19 GGWFNPAPNGAGGSTNVLTMPAEE2407 pKa = 4.28 NGNNVLWLNDD2417 pKa = 3.75 GQVATQVVGAANLGRR2432 pKa = 11.84 YY2433 pKa = 7.19 EE2434 pKa = 4.19 LNIEE2438 pKa = 4.02 VGDD2441 pKa = 3.93 RR2442 pKa = 11.84 TDD2444 pKa = 3.53 SNLNGMPDD2452 pKa = 3.78 FAVRR2456 pKa = 11.84 LYY2458 pKa = 11.21 AGAPLIAEE2466 pKa = 4.39 QIVEE2470 pKa = 4.19 HH2471 pKa = 5.97 TTTDD2475 pKa = 3.6 GYY2477 pKa = 11.54 SWTLVKK2483 pKa = 10.3 IEE2485 pKa = 4.08 GLVDD2489 pKa = 3.47 NSLSGQPLRR2498 pKa = 11.84 IEE2500 pKa = 4.2 VANLEE2505 pKa = 4.33 EE2506 pKa = 4.78 FGSGGDD2512 pKa = 3.76 FPQQVQVNFDD2522 pKa = 3.89 NVTLNYY2528 pKa = 10.13 VPTQTLTSIDD2538 pKa = 3.66 EE2539 pKa = 4.3 DD2540 pKa = 3.53 AVANNGQTVASILGASVTDD2559 pKa = 3.97 ADD2561 pKa = 3.96 HH2562 pKa = 7.04 GAVQGIAITHH2572 pKa = 6.5 TDD2574 pKa = 3.23 TSNGAWQYY2582 pKa = 11.4 SSDD2585 pKa = 4.68 GGAHH2589 pKa = 4.94 WTDD2592 pKa = 3.24 IGSHH2596 pKa = 6.57 SITSALLLSGTDD2608 pKa = 3.26 LVRR2611 pKa = 11.84 FVPDD2615 pKa = 5.19 GIHH2618 pKa = 5.82 GTTAGFDD2625 pKa = 3.58 YY2626 pKa = 10.83 LAWDD2630 pKa = 3.61 QTSGTLHH2637 pKa = 7.16 DD2638 pKa = 4.17 VANVTNRR2645 pKa = 11.84 GGTTAFSEE2653 pKa = 4.61 DD2654 pKa = 3.28 TAHH2657 pKa = 7.44 AVITVTDD2664 pKa = 3.91 VNDD2667 pKa = 4.15 APTPMADD2674 pKa = 2.96 SVSLIEE2680 pKa = 4.81 TSATDD2685 pKa = 3.39 AGSVPVNGKK2694 pKa = 10.7 GNVLANDD2701 pKa = 4.02 HH2702 pKa = 7.19 DD2703 pKa = 4.56 VDD2705 pKa = 4.26 GDD2707 pKa = 3.98 PLSVILVRR2715 pKa = 11.84 AANVSPEE2722 pKa = 3.99 AQEE2725 pKa = 3.92 APQYY2729 pKa = 8.4 ITNGEE2734 pKa = 4.03 ATAHH2738 pKa = 4.92 GTYY2741 pKa = 10.77 GNLVMHH2747 pKa = 7.51 ADD2749 pKa = 3.52 GSYY2752 pKa = 10.08 TYY2754 pKa = 9.67 TPNAAYY2760 pKa = 9.36 DD2761 pKa = 3.82 ALPEE2765 pKa = 4.4 GVQVTDD2771 pKa = 2.94 IFKK2774 pKa = 10.63 FGVSDD2779 pKa = 3.29 GHH2781 pKa = 7.58 GGDD2784 pKa = 3.25 VTSYY2788 pKa = 9.5 LTVNITGANDD2798 pKa = 3.4 APVIDD2803 pKa = 4.58 LSGPNVPGNDD2813 pKa = 3.19 ATATAVEE2820 pKa = 4.64 QLPQWFMDD2828 pKa = 3.21 KK2829 pKa = 10.52 ATISDD2834 pKa = 3.62 VDD2836 pKa = 4.01 STTMASLTVTLSSRR2850 pKa = 11.84 PDD2852 pKa = 3.02 GGAEE2856 pKa = 3.79 YY2857 pKa = 10.87 LSLNTAAQNALTAHH2871 pKa = 6.46 SLSSSYY2877 pKa = 11.31 DD2878 pKa = 3.14 ATTGALIVTGIAAAAVYY2895 pKa = 7.58 QTILDD2900 pKa = 3.77 GVVYY2904 pKa = 10.65 LNTSDD2909 pKa = 4.62 NPSTTDD2915 pKa = 2.85 RR2916 pKa = 11.84 TINVTVNDD2924 pKa = 5.72 GISNSPLQNVTLHH2937 pKa = 5.47 VWASNDD2943 pKa = 3.31 APEE2946 pKa = 4.88 LAGALHH2952 pKa = 6.59 ASVGEE2957 pKa = 4.38 GGTHH2961 pKa = 6.64 VIQPAEE2967 pKa = 4.31 LGFTDD2972 pKa = 4.63 PDD2974 pKa = 4.85 DD2975 pKa = 3.83 IASGVTFIVSDD2986 pKa = 3.64 QQNGIVKK2993 pKa = 10.08 LDD2995 pKa = 3.7 GVASLSFTGQQLADD3009 pKa = 3.36 GHH3011 pKa = 5.2 VTFVHH3016 pKa = 6.86 DD3017 pKa = 5.36 GSDD3020 pKa = 3.03 TTAASFAVKK3029 pKa = 10.61 VEE3031 pKa = 4.76 DD3032 pKa = 3.98 GNEE3035 pKa = 4.06 DD3036 pKa = 3.13 NSVPVSQTFHH3046 pKa = 7.99 LDD3048 pKa = 3.27 VTPVNDD3054 pKa = 3.59 APVIDD3059 pKa = 4.28 SHH3061 pKa = 8.59 NGDD3064 pKa = 3.73 NLVALSMFEE3073 pKa = 3.84 NATAVTTVHH3082 pKa = 6.85 AADD3085 pKa = 4.82 PDD3087 pKa = 4.52 SPTLTYY3093 pKa = 9.86 TISGGADD3100 pKa = 2.71 ASLFHH3105 pKa = 7.45 VDD3107 pKa = 3.65 ASTGALSFVNAPDD3120 pKa = 3.72 FEE3122 pKa = 4.73 TPHH3125 pKa = 7.34 DD3126 pKa = 4.45 AGLDD3130 pKa = 3.14 NHH3132 pKa = 6.65 YY3133 pKa = 10.61 NVTVTASDD3141 pKa = 3.36 GTLFDD3146 pKa = 3.7 TQSFDD3151 pKa = 4.35 VEE3153 pKa = 4.44 VKK3155 pKa = 10.95 DD3156 pKa = 5.29 DD3157 pKa = 3.87 VEE3159 pKa = 4.67 TYY3161 pKa = 11.19 SPVTVTNDD3169 pKa = 2.76 NPYY3172 pKa = 11.04 YY3173 pKa = 10.88 AFSDD3177 pKa = 3.76 NANAPAGQPVAIHH3190 pKa = 6.55 FDD3192 pKa = 3.4 ADD3194 pKa = 4.31 DD3195 pKa = 3.79 IFGGGSDD3202 pKa = 3.37 TFSYY3206 pKa = 10.28 MFTQVYY3212 pKa = 10.59 SSTSTNWLTVDD3223 pKa = 3.9 PTNGTITGDD3232 pKa = 3.81 PDD3234 pKa = 3.49 HH3235 pKa = 7.11 LSALTLYY3242 pKa = 10.5 RR3243 pKa = 11.84 IDD3245 pKa = 4.65 AMDD3248 pKa = 3.84 SVTHH3252 pKa = 4.95 QTSFTYY3258 pKa = 10.68 VAFSALNDD3266 pKa = 3.53 AGTLVTVANDD3276 pKa = 3.84 TVGPLPNNGNNGDD3289 pKa = 3.8 NLYY3292 pKa = 10.51 LVSGNQSHH3300 pKa = 6.8 DD3301 pKa = 3.79 TNSGPNEE3308 pKa = 3.97 DD3309 pKa = 4.34 VLIGDD3314 pKa = 4.82 AANNMMNGGNLNDD3327 pKa = 4.3 ALYY3330 pKa = 10.7 GGNGNDD3336 pKa = 3.77 TLSGDD3341 pKa = 3.52 TSTDD3345 pKa = 3.55 FLNGGNGDD3353 pKa = 3.65 DD3354 pKa = 4.29 TLNGGNSGDD3363 pKa = 3.75 FLIGGEE3369 pKa = 4.94 GNDD3372 pKa = 4.14 TLNGDD3377 pKa = 3.5 TDD3379 pKa = 3.47 TDD3381 pKa = 3.98 YY3382 pKa = 12.0 LLGGNGNDD3390 pKa = 3.88 TLNGGNGNDD3399 pKa = 3.72 FLYY3402 pKa = 11.28 GEE3404 pKa = 5.2 AGDD3407 pKa = 4.48 DD3408 pKa = 3.88 HH3409 pKa = 8.22 LSGDD3413 pKa = 3.87 TGVDD3417 pKa = 3.15 TLYY3420 pKa = 11.12 GGSGKK3425 pKa = 8.77 DD3426 pKa = 3.28 TLNGGNGDD3434 pKa = 3.79 DD3435 pKa = 4.23 FLYY3438 pKa = 11.0 GGAGDD3443 pKa = 5.21 DD3444 pKa = 4.6 DD3445 pKa = 5.85 LNGGTGVDD3453 pKa = 3.38 TFYY3456 pKa = 11.51 GGLGKK3461 pKa = 9.08 DD3462 pKa = 3.77 TITGGDD3468 pKa = 3.49 MNGHH3472 pKa = 6.94 DD3473 pKa = 4.18 KK3474 pKa = 11.44 YY3475 pKa = 11.27 IFAEE3479 pKa = 5.09 FGADD3483 pKa = 3.42 NADD3486 pKa = 3.6 LLEE3489 pKa = 4.3 TFRR3492 pKa = 11.84 SNDD3495 pKa = 3.56 VIDD3498 pKa = 4.53 LSSLLGAEE3506 pKa = 4.5 TGASGTNIGSYY3517 pKa = 9.24 VHH3519 pKa = 6.81 LTQQSNNDD3527 pKa = 3.16 VLIQIDD3533 pKa = 3.54 ITGSGNFAGSAHH3545 pKa = 7.11 DD3546 pKa = 3.62 VATLQGYY3553 pKa = 7.93 GSPSVNDD3560 pKa = 3.32 IVRR3563 pKa = 11.84 VAFQGQEE3570 pKa = 3.73 HH3571 pKa = 5.6 QMMVV3575 pKa = 2.94
Molecular weight: 369.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.732
IPC_protein 3.808
Toseland 3.567
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.77
Rodwell 3.63
Grimsley 3.478
Solomon 3.808
Lehninger 3.77
Nozaki 3.91
DTASelect 4.215
Thurlkill 3.617
EMBOSS 3.77
Sillero 3.935
Patrickios 1.647
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.824
Protein with the highest isoelectric point:
>tr|A0A0Q6Z815|A0A0Q6Z815_9BRAD Molybdenum ABC transporter ATP-binding protein OS=Afipia sp. Root123D2 OX=1736436 GN=ASC80_17390 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.55 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 LATTGGRR28 pKa = 11.84 KK29 pKa = 9.06 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4397
0
4397
1357450
26
3575
308.7
33.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.153 ± 0.043
0.824 ± 0.012
5.674 ± 0.031
5.224 ± 0.03
3.826 ± 0.023
8.286 ± 0.037
2.049 ± 0.018
5.639 ± 0.031
3.93 ± 0.032
9.723 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.538 ± 0.019
2.829 ± 0.019
5.146 ± 0.025
3.104 ± 0.021
6.799 ± 0.041
5.669 ± 0.027
5.489 ± 0.025
7.556 ± 0.036
1.281 ± 0.015
2.259 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here