Tadarida brasiliensis circovirus 1
Average proteome isoelectric point is 8.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N9P0D8|A0A0N9P0D8_9CIRC Capsid protein OS=Tadarida brasiliensis circovirus 1 OX=1732201 GN=cap PE=3 SV=1
MM1 pKa = 7.11 NVKK4 pKa = 10.23 RR5 pKa = 11.84 KK6 pKa = 9.86 YY7 pKa = 9.09 NAKK10 pKa = 10.4 KK11 pKa = 10.43 GDD13 pKa = 3.72 EE14 pKa = 4.37 TPGRR18 pKa = 11.84 RR19 pKa = 11.84 WCFTINNYY27 pKa = 8.5 GAPDD31 pKa = 3.99 LEE33 pKa = 4.73 CVNEE37 pKa = 3.95 AFRR40 pKa = 11.84 CEE42 pKa = 4.03 DD43 pKa = 3.05 VVYY46 pKa = 10.3 AICGKK51 pKa = 10.31 EE52 pKa = 3.9 KK53 pKa = 10.22 GAKK56 pKa = 7.39 GTPHH60 pKa = 6.27 LQGFIHH66 pKa = 6.44 FTGNWRR72 pKa = 11.84 FNRR75 pKa = 11.84 VRR77 pKa = 11.84 NLLGGRR83 pKa = 11.84 AHH85 pKa = 6.94 IEE87 pKa = 3.84 KK88 pKa = 10.73 ARR90 pKa = 11.84 GNDD93 pKa = 3.78 DD94 pKa = 3.3 QNKK97 pKa = 9.03 AYY99 pKa = 10.16 CSKK102 pKa = 10.83 EE103 pKa = 3.66 EE104 pKa = 4.14 TYY106 pKa = 11.25 LEE108 pKa = 4.24 VGTPQFQGKK117 pKa = 9.93 RR118 pKa = 11.84 NDD120 pKa = 3.5 LGRR123 pKa = 11.84 VASALEE129 pKa = 4.16 SGATLSEE136 pKa = 4.32 VARR139 pKa = 11.84 ASPEE143 pKa = 3.6 VFIRR147 pKa = 11.84 YY148 pKa = 8.71 GRR150 pKa = 11.84 GLRR153 pKa = 11.84 DD154 pKa = 3.38 YY155 pKa = 9.8 MNVRR159 pKa = 11.84 GLVKK163 pKa = 10.32 PRR165 pKa = 11.84 DD166 pKa = 3.78 FKK168 pKa = 11.2 TEE170 pKa = 4.0 VIVLVGEE177 pKa = 4.32 PGSGKK182 pKa = 10.21 SKK184 pKa = 10.51 YY185 pKa = 10.56 ANEE188 pKa = 4.4 LPGSKK193 pKa = 9.24 YY194 pKa = 9.34 WKK196 pKa = 9.87 PRR198 pKa = 11.84 GQWWDD203 pKa = 3.52 GYY205 pKa = 9.64 NGEE208 pKa = 5.22 DD209 pKa = 4.59 IVVLDD214 pKa = 5.32 DD215 pKa = 3.98 FYY217 pKa = 11.83 GWVPYY222 pKa = 10.67 DD223 pKa = 3.39 EE224 pKa = 5.15 LLRR227 pKa = 11.84 IGDD230 pKa = 4.37 RR231 pKa = 11.84 YY232 pKa = 8.95 PLKK235 pKa = 10.68 VQVKK239 pKa = 8.31 GAFVEE244 pKa = 5.43 FTSKK248 pKa = 10.21 MLVITSNKK256 pKa = 9.43 RR257 pKa = 11.84 PEE259 pKa = 3.55 EE260 pKa = 4.22 WYY262 pKa = 10.23 DD263 pKa = 3.27 KK264 pKa = 11.23 EE265 pKa = 5.15 KK266 pKa = 10.78 IADD269 pKa = 3.62 QSAMWRR275 pKa = 11.84 RR276 pKa = 11.84 FDD278 pKa = 3.87 KK279 pKa = 10.3 MYY281 pKa = 9.39 YY282 pKa = 9.26 CEE284 pKa = 4.17 RR285 pKa = 11.84 GEE287 pKa = 3.93 PVKK290 pKa = 10.55 AYY292 pKa = 8.08 PHH294 pKa = 5.71 EE295 pKa = 4.24 WKK297 pKa = 10.61 EE298 pKa = 3.93 FEE300 pKa = 3.95 TGYY303 pKa = 11.46
Molecular weight: 35.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.051
IPC2_protein 8.156
IPC_protein 8.053
Toseland 8.287
ProMoST 8.624
Dawson 8.843
Bjellqvist 8.916
Wikipedia 8.916
Rodwell 8.916
Grimsley 8.375
Solomon 8.946
Lehninger 8.931
Nozaki 9.004
DTASelect 8.741
Thurlkill 8.814
EMBOSS 9.004
Sillero 9.048
Patrickios 4.406
IPC_peptide 8.946
IPC2_peptide 7.658
IPC2.peptide.svr19 7.735
Protein with the highest isoelectric point:
>tr|A0A0N9P0D8|A0A0N9P0D8_9CIRC Capsid protein OS=Tadarida brasiliensis circovirus 1 OX=1732201 GN=cap PE=3 SV=1
MM1 pKa = 7.27 PRR3 pKa = 11.84 SRR5 pKa = 11.84 RR6 pKa = 11.84 HH7 pKa = 4.13 RR8 pKa = 11.84 WRR10 pKa = 11.84 RR11 pKa = 11.84 NQWFKK16 pKa = 10.05 RR17 pKa = 11.84 WRR19 pKa = 11.84 RR20 pKa = 11.84 SRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 GHH26 pKa = 5.42 TFGTRR31 pKa = 11.84 RR32 pKa = 11.84 WRR34 pKa = 11.84 QKK36 pKa = 10.18 NGIYY40 pKa = 10.3 NFKK43 pKa = 10.63 FKK45 pKa = 11.02 ANSLQTVNKK54 pKa = 9.06 TSNQGYY60 pKa = 7.73 FTFSLAAACPRR71 pKa = 11.84 AFSTYY76 pKa = 9.1 FDD78 pKa = 3.56 MYY80 pKa = 10.15 RR81 pKa = 11.84 IRR83 pKa = 11.84 KK84 pKa = 8.71 VRR86 pKa = 11.84 VQWLPMSGINEE97 pKa = 3.84 PRR99 pKa = 11.84 VWGATIIDD107 pKa = 3.72 LTGRR111 pKa = 11.84 DD112 pKa = 3.44 TTLPSTGNTDD122 pKa = 3.67 FTIDD126 pKa = 3.59 DD127 pKa = 3.55 VTRR130 pKa = 11.84 RR131 pKa = 11.84 IWNPTRR137 pKa = 11.84 LHH139 pKa = 5.86 SRR141 pKa = 11.84 YY142 pKa = 7.56 FTPKK146 pKa = 9.85 PEE148 pKa = 3.95 IAIKK152 pKa = 9.27 TVSSEE157 pKa = 4.31 AIQPNNPRR165 pKa = 11.84 NQLWLDD171 pKa = 3.45 AKK173 pKa = 11.12 YY174 pKa = 10.59 PDD176 pKa = 4.51 AKK178 pKa = 10.7 HH179 pKa = 6.27 HH180 pKa = 5.94 GCAFFFGQTDD190 pKa = 3.91 LGDD193 pKa = 3.53 EE194 pKa = 4.33 SYY196 pKa = 11.31 KK197 pKa = 10.73 FQFLVTYY204 pKa = 8.22 YY205 pKa = 10.02 IQFRR209 pKa = 11.84 QFAGSLAPSS218 pKa = 3.52
Molecular weight: 25.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.282
IPC2_protein 10.072
IPC_protein 11.111
Toseland 11.125
ProMoST 11.301
Dawson 11.184
Bjellqvist 11.038
Wikipedia 11.535
Rodwell 11.199
Grimsley 11.228
Solomon 11.491
Lehninger 11.418
Nozaki 11.111
DTASelect 11.038
Thurlkill 11.125
EMBOSS 11.579
Sillero 11.14
Patrickios 10.906
IPC_peptide 11.491
IPC2_peptide 10.35
IPC2.peptide.svr19 8.565
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
521
218
303
260.5
30.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.95 ± 0.008
1.536 ± 0.377
5.182 ± 0.363
5.758 ± 2.393
5.758 ± 1.245
8.061 ± 1.56
1.727 ± 0.345
4.031 ± 0.619
7.102 ± 1.254
5.374 ± 0.2
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.536 ± 0.097
5.182 ± 0.197
4.99 ± 0.314
3.455 ± 0.971
9.789 ± 1.584
4.798 ± 0.99
5.758 ± 1.804
5.566 ± 1.437
3.263 ± 0.248
5.182 ± 0.643
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here