Pea streak virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H3YGY3|A0A0H3YGY3_9VIRU Helicase OS=Pea streak virus OX=157777 PE=3 SV=1
MM1 pKa = 7.97DD2 pKa = 4.01VFINNLLEE10 pKa = 4.3CGFRR14 pKa = 11.84RR15 pKa = 11.84NKK17 pKa = 10.03IVFDD21 pKa = 3.98LPIVVLAVPGAGKK34 pKa = 8.68TSSIRR39 pKa = 11.84RR40 pKa = 11.84LLRR43 pKa = 11.84QDD45 pKa = 3.17SRR47 pKa = 11.84FEE49 pKa = 3.58AWTFGVADD57 pKa = 3.63HH58 pKa = 6.95HH59 pKa = 6.31NCSGRR64 pKa = 11.84FIKK67 pKa = 10.64GISEE71 pKa = 4.04EE72 pKa = 4.37SKK74 pKa = 10.12PDD76 pKa = 3.47PSKK79 pKa = 10.97FILIDD84 pKa = 3.22EE85 pKa = 4.76FQRR88 pKa = 11.84GDD90 pKa = 3.34WEE92 pKa = 4.14KK93 pKa = 10.89LKK95 pKa = 10.77PFAIFGDD102 pKa = 3.93IAQLMLKK109 pKa = 10.2SSAAFEE115 pKa = 4.84SVFSKK120 pKa = 10.64CSSHH124 pKa = 6.81RR125 pKa = 11.84VPLPIVKK132 pKa = 10.13LLQEE136 pKa = 4.68LDD138 pKa = 3.88FEE140 pKa = 4.57ITSEE144 pKa = 4.27RR145 pKa = 11.84EE146 pKa = 3.62GVLEE150 pKa = 3.88VRR152 pKa = 11.84TLLGSEE158 pKa = 4.17PEE160 pKa = 4.28GVVTCFEE167 pKa = 4.49SEE169 pKa = 4.04VCEE172 pKa = 3.94FLDD175 pKa = 4.34YY176 pKa = 11.39NQINHH181 pKa = 7.2KK182 pKa = 10.38SPADD186 pKa = 3.54IIGLEE191 pKa = 4.26FPTVSLVISGKK202 pKa = 9.0SAIGVHH208 pKa = 5.57RR209 pKa = 11.84AEE211 pKa = 5.86FYY213 pKa = 10.4ICCTRR218 pKa = 11.84ATEE221 pKa = 4.07KK222 pKa = 11.07LLIITPEE229 pKa = 3.72PEE231 pKa = 4.17GFHH234 pKa = 6.16NCSNAIDD241 pKa = 3.94STSS244 pKa = 2.68

Molecular weight: 27.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H3YH99|A0A0H3YH99_9VIRU 7 kDa protein OS=Pea streak virus OX=157777 PE=3 SV=1
MM1 pKa = 6.88STSPSTLIRR10 pKa = 11.84RR11 pKa = 11.84TLWLEE16 pKa = 3.64LTEE19 pKa = 4.38IADD22 pKa = 3.81TRR24 pKa = 11.84VMMRR28 pKa = 11.84LLPVVCSVVEE38 pKa = 3.79PRR40 pKa = 11.84KK41 pKa = 9.93IGVIMRR47 pKa = 11.84AKK49 pKa = 10.1CVRR52 pKa = 11.84LLVKK56 pKa = 10.42PKK58 pKa = 10.66

Molecular weight: 6.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2674

58

1908

445.7

50.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.956 ± 1.086

2.58 ± 0.351

5.273 ± 0.723

7.741 ± 1.142

5.497 ± 0.654

6.133 ± 0.366

2.169 ± 0.34

6.47 ± 1.147

6.395 ± 0.285

10.845 ± 1.111

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.982 ± 0.401

4.525 ± 0.767

3.441 ± 0.666

3.067 ± 0.585

5.572 ± 0.607

7.031 ± 0.666

4.525 ± 0.35

5.684 ± 0.76

1.01 ± 0.228

3.104 ± 0.803
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski