Amphibola crenata associated bacilladnavirus 1
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P8YT79|A0A1P8YT79_9VIRU p2 OS=Amphibola crenata associated bacilladnavirus 1 OX=1941435 PE=4 SV=1
MM1 pKa = 7.71 AIINTHH7 pKa = 5.69 MPFSDD12 pKa = 3.54 NGEE15 pKa = 4.4 LIQVDD20 pKa = 3.83 MQKK23 pKa = 11.1 ALSCLMFFNTLGIYY37 pKa = 10.5 NDD39 pKa = 3.09 ATYY42 pKa = 11.09 YY43 pKa = 10.68 GIKK46 pKa = 10.12 LWMDD50 pKa = 3.44 AYY52 pKa = 8.78 MTIGNAPMPSGVLFKK67 pKa = 11.06 SPLGGYY73 pKa = 9.69 VGFTQLMAEE82 pKa = 5.26 LEE84 pKa = 4.41 QEE86 pKa = 3.9 WAARR90 pKa = 11.84 EE91 pKa = 4.12 AFHH94 pKa = 6.46 LTIDD98 pKa = 3.9 EE99 pKa = 4.66 EE100 pKa = 4.38 EE101 pKa = 4.36 VDD103 pKa = 4.27 DD104 pKa = 6.29 DD105 pKa = 4.45 EE106 pKa = 4.92 STVTGPVTQPEE117 pKa = 4.49 YY118 pKa = 10.97 EE119 pKa = 4.5 PYY121 pKa = 11.09 ALDD124 pKa = 5.37 DD125 pKa = 5.19 DD126 pKa = 5.31 DD127 pKa = 7.36 SIDD130 pKa = 3.46 AMLNIPVNIDD140 pKa = 3.23 FNLGFFDD147 pKa = 5.25 EE148 pKa = 4.52 MDD150 pKa = 3.77 EE151 pKa = 4.32 EE152 pKa = 4.75 DD153 pKa = 3.61 VV154 pKa = 3.85
Molecular weight: 17.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.89
IPC2_protein 3.478
IPC_protein 3.465
Toseland 3.249
ProMoST 3.643
Dawson 3.465
Bjellqvist 3.617
Wikipedia 3.401
Rodwell 3.3
Grimsley 3.172
Solomon 3.439
Lehninger 3.401
Nozaki 3.592
DTASelect 3.795
Thurlkill 3.312
EMBOSS 3.414
Sillero 3.592
Patrickios 0.362
IPC_peptide 3.439
IPC2_peptide 3.567
IPC2.peptide.svr19 3.635
Protein with the highest isoelectric point:
>tr|A0A1P8YT82|A0A1P8YT82_9VIRU p1 OS=Amphibola crenata associated bacilladnavirus 1 OX=1941435 PE=4 SV=1
MM1 pKa = 7.61 PKK3 pKa = 9.88 KK4 pKa = 9.88 SSRR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 TTATTKK16 pKa = 10.45 KK17 pKa = 10.09 KK18 pKa = 10.62 SSSKK22 pKa = 10.14 KK23 pKa = 9.03 AAKK26 pKa = 9.89 KK27 pKa = 10.67 GPTTKK32 pKa = 10.52 KK33 pKa = 9.64 VVKK36 pKa = 10.13 KK37 pKa = 11.03 VMGASAIALKK47 pKa = 10.43 PLVSPFDD54 pKa = 4.04 RR55 pKa = 11.84 SNMQPKK61 pKa = 10.01 IPDD64 pKa = 3.59 GKK66 pKa = 10.06 VGEE69 pKa = 4.41 SVGLKK74 pKa = 8.92 FTMTKK79 pKa = 10.01 EE80 pKa = 4.12 YY81 pKa = 11.02 SNKK84 pKa = 9.18 TLTTMHH90 pKa = 6.8 AMLFPGQSGGLFVSGCQNVNGINRR114 pKa = 11.84 EE115 pKa = 3.95 VQAYY119 pKa = 9.63 ANAGGFSYY127 pKa = 10.14 TYY129 pKa = 11.44 DD130 pKa = 3.59 NTTKK134 pKa = 10.15 MALLTANEE142 pKa = 5.22 KK143 pKa = 8.12 YY144 pKa = 10.35 HH145 pKa = 5.35 SWRR148 pKa = 11.84 IVSQAAKK155 pKa = 10.61 FEE157 pKa = 4.38 LLNPAEE163 pKa = 4.99 EE164 pKa = 4.94 DD165 pKa = 3.82 DD166 pKa = 4.36 GWWEE170 pKa = 3.92 AVRR173 pKa = 11.84 ITDD176 pKa = 3.57 MLSLFDD182 pKa = 3.65 YY183 pKa = 10.72 HH184 pKa = 8.42 VEE186 pKa = 3.82 QHH188 pKa = 5.71 SNKK191 pKa = 9.19 TGRR194 pKa = 11.84 LQGVFVPFLGLLNLEE209 pKa = 4.55 DD210 pKa = 3.75 RR211 pKa = 11.84 ALTAEE216 pKa = 4.02 RR217 pKa = 11.84 TYY219 pKa = 11.35 EE220 pKa = 3.95 SGRR223 pKa = 11.84 LKK225 pKa = 10.54 DD226 pKa = 2.96 IHH228 pKa = 7.59 KK229 pKa = 10.52 RR230 pKa = 11.84 EE231 pKa = 3.73 FHH233 pKa = 5.66 LRR235 pKa = 11.84 QVKK238 pKa = 10.85 DD239 pKa = 3.55 EE240 pKa = 4.14 VDD242 pKa = 4.08 FTQLCSPIALEE253 pKa = 4.67 GVQEE257 pKa = 4.36 TGAGTSGSPFVLANGAGANAGEE279 pKa = 4.35 SFGMQNYY286 pKa = 9.46 NAMRR290 pKa = 11.84 NYY292 pKa = 9.84 IDD294 pKa = 4.92 FGYY297 pKa = 10.76 DD298 pKa = 3.22 CIYY301 pKa = 10.8 FRR303 pKa = 11.84 FHH305 pKa = 6.77 CRR307 pKa = 11.84 DD308 pKa = 3.22 PASATRR314 pKa = 11.84 LLTHH318 pKa = 6.1 VVSNQEE324 pKa = 3.41 IQYY327 pKa = 9.84 SSEE330 pKa = 3.95 SGEE333 pKa = 4.83 SRR335 pKa = 11.84 FQTANTRR342 pKa = 11.84 HH343 pKa = 5.51 AQVDD347 pKa = 3.81 TVMANRR353 pKa = 11.84 SNGGATTSVPSTSRR367 pKa = 11.84 HH368 pKa = 4.66 NPHH371 pKa = 6.91 RR372 pKa = 4.38
Molecular weight: 41.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.915
IPC2_protein 8.916
IPC_protein 8.829
Toseland 9.589
ProMoST 9.385
Dawson 9.838
Bjellqvist 9.545
Wikipedia 9.999
Rodwell 10.189
Grimsley 9.911
Solomon 9.867
Lehninger 9.838
Nozaki 9.663
DTASelect 9.516
Thurlkill 9.692
EMBOSS 10.014
Sillero 9.78
Patrickios 5.436
IPC_peptide 9.867
IPC2_peptide 8.126
IPC2.peptide.svr19 7.996
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1260
154
513
315.0
35.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.952 ± 0.985
1.111 ± 0.344
7.381 ± 1.687
6.905 ± 0.784
3.81 ± 0.64
6.111 ± 0.659
3.254 ± 0.841
4.524 ± 0.68
7.46 ± 1.393
5.714 ± 0.754
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.095 ± 0.614
4.841 ± 0.661
5.397 ± 0.629
3.81 ± 0.131
5.397 ± 0.803
6.587 ± 0.797
6.825 ± 0.734
6.27 ± 0.584
2.063 ± 0.64
3.492 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here