Amino acid dipepetide frequency for Streptomyces phage Romero

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.084AlaAla: 11.084 ± 1.248
0.749AlaCys: 0.749 ± 0.236
5.243AlaAsp: 5.243 ± 0.743
6.441AlaGlu: 6.441 ± 0.721
2.996AlaPhe: 2.996 ± 0.479
9.736AlaGly: 9.736 ± 1.008
1.348AlaHis: 1.348 ± 0.329
3.37AlaIle: 3.37 ± 0.629
5.767AlaLys: 5.767 ± 0.84
7.49AlaLeu: 7.49 ± 0.673
2.097AlaMet: 2.097 ± 0.389
3.969AlaAsn: 3.969 ± 0.556
3.895AlaPro: 3.895 ± 0.588
5.243AlaGln: 5.243 ± 0.88
4.643AlaArg: 4.643 ± 0.596
6.216AlaSer: 6.216 ± 0.552
6.815AlaThr: 6.815 ± 0.913
7.49AlaVal: 7.49 ± 1.038
2.097AlaTrp: 2.097 ± 0.448
3.071AlaTyr: 3.071 ± 0.493
0.0AlaXaa: 0.0 ± 0.0
Cys
0.674CysAla: 0.674 ± 0.227
0.0CysCys: 0.0 ± 0.0
0.3CysAsp: 0.3 ± 0.201
0.449CysGlu: 0.449 ± 0.212
0.15CysPhe: 0.15 ± 0.126
0.449CysGly: 0.449 ± 0.248
0.075CysHis: 0.075 ± 0.068
0.524CysIle: 0.524 ± 0.204
0.449CysLys: 0.449 ± 0.22
0.749CysLeu: 0.749 ± 0.258
0.3CysMet: 0.3 ± 0.168
0.15CysAsn: 0.15 ± 0.097
0.524CysPro: 0.524 ± 0.192
0.374CysGln: 0.374 ± 0.204
0.374CysArg: 0.374 ± 0.204
0.674CysSer: 0.674 ± 0.233
0.449CysThr: 0.449 ± 0.293
0.524CysVal: 0.524 ± 0.231
0.225CysTrp: 0.225 ± 0.135
0.15CysTyr: 0.15 ± 0.098
0.0CysXaa: 0.0 ± 0.0
Asp
6.216AspAla: 6.216 ± 0.887
0.225AspCys: 0.225 ± 0.124
3.969AspAsp: 3.969 ± 0.56
3.82AspGlu: 3.82 ± 0.583
2.846AspPhe: 2.846 ± 0.444
5.168AspGly: 5.168 ± 0.615
1.049AspHis: 1.049 ± 0.24
3.595AspIle: 3.595 ± 0.504
2.696AspLys: 2.696 ± 0.502
4.344AspLeu: 4.344 ± 0.61
2.172AspMet: 2.172 ± 0.451
2.172AspAsn: 2.172 ± 0.437
4.643AspPro: 4.643 ± 0.632
2.322AspGln: 2.322 ± 0.495
3.071AspArg: 3.071 ± 0.474
3.071AspSer: 3.071 ± 0.357
2.921AspThr: 2.921 ± 0.393
4.868AspVal: 4.868 ± 0.664
1.049AspTrp: 1.049 ± 0.267
2.771AspTyr: 2.771 ± 0.523
0.0AspXaa: 0.0 ± 0.0
Glu
5.467GluAla: 5.467 ± 0.652
0.449GluCys: 0.449 ± 0.209
4.044GluAsp: 4.044 ± 0.659
4.269GluGlu: 4.269 ± 0.591
2.172GluPhe: 2.172 ± 0.447
4.868GluGly: 4.868 ± 0.509
1.198GluHis: 1.198 ± 0.33
2.921GluIle: 2.921 ± 0.426
3.22GluLys: 3.22 ± 0.513
5.243GluLeu: 5.243 ± 0.681
1.348GluMet: 1.348 ± 0.335
2.546GluAsn: 2.546 ± 0.481
1.648GluPro: 1.648 ± 0.359
2.846GluGln: 2.846 ± 0.408
2.921GluArg: 2.921 ± 0.464
2.696GluSer: 2.696 ± 0.449
2.696GluThr: 2.696 ± 0.498
5.018GluVal: 5.018 ± 0.7
0.974GluTrp: 0.974 ± 0.237
2.771GluTyr: 2.771 ± 0.461
0.0GluXaa: 0.0 ± 0.0
Phe
2.996PheAla: 2.996 ± 0.525
0.3PheCys: 0.3 ± 0.167
2.771PheAsp: 2.771 ± 0.482
2.472PheGlu: 2.472 ± 0.457
0.974PhePhe: 0.974 ± 0.205
3.52PheGly: 3.52 ± 0.488
0.824PheHis: 0.824 ± 0.249
1.049PheIle: 1.049 ± 0.251
1.423PheLys: 1.423 ± 0.308
1.797PheLeu: 1.797 ± 0.38
0.899PheMet: 0.899 ± 0.225
1.872PheAsn: 1.872 ± 0.312
1.273PhePro: 1.273 ± 0.401
2.022PheGln: 2.022 ± 0.366
1.872PheArg: 1.872 ± 0.405
2.172PheSer: 2.172 ± 0.424
2.322PheThr: 2.322 ± 0.36
1.947PheVal: 1.947 ± 0.368
0.449PheTrp: 0.449 ± 0.191
1.273PheTyr: 1.273 ± 0.333
0.0PheXaa: 0.0 ± 0.0
Gly
9.137GlyAla: 9.137 ± 1.082
0.749GlyCys: 0.749 ± 0.262
5.318GlyAsp: 5.318 ± 0.541
5.767GlyGlu: 5.767 ± 0.734
3.071GlyPhe: 3.071 ± 0.35
8.913GlyGly: 8.913 ± 0.965
1.423GlyHis: 1.423 ± 0.39
4.194GlyIle: 4.194 ± 0.745
5.542GlyLys: 5.542 ± 0.65
5.617GlyLeu: 5.617 ± 0.718
3.071GlyMet: 3.071 ± 0.511
3.37GlyAsn: 3.37 ± 0.537
2.696GlyPro: 2.696 ± 0.382
4.269GlyGln: 4.269 ± 0.762
4.194GlyArg: 4.194 ± 0.392
5.842GlySer: 5.842 ± 0.625
6.067GlyThr: 6.067 ± 0.766
5.692GlyVal: 5.692 ± 0.656
1.648GlyTrp: 1.648 ± 0.294
3.22GlyTyr: 3.22 ± 0.577
0.0GlyXaa: 0.0 ± 0.0
His
1.273HisAla: 1.273 ± 0.259
0.225HisCys: 0.225 ± 0.134
1.123HisAsp: 1.123 ± 0.337
1.198HisGlu: 1.198 ± 0.288
0.674HisPhe: 0.674 ± 0.212
1.872HisGly: 1.872 ± 0.433
0.374HisHis: 0.374 ± 0.16
1.198HisIle: 1.198 ± 0.29
0.824HisLys: 0.824 ± 0.31
1.123HisLeu: 1.123 ± 0.289
0.449HisMet: 0.449 ± 0.177
0.3HisAsn: 0.3 ± 0.158
0.374HisPro: 0.374 ± 0.208
0.449HisGln: 0.449 ± 0.164
0.899HisArg: 0.899 ± 0.252
1.198HisSer: 1.198 ± 0.342
0.824HisThr: 0.824 ± 0.231
0.749HisVal: 0.749 ± 0.207
0.3HisTrp: 0.3 ± 0.213
0.674HisTyr: 0.674 ± 0.254
0.0HisXaa: 0.0 ± 0.0
Ile
3.445IleAla: 3.445 ± 0.504
0.3IleCys: 0.3 ± 0.128
2.771IleAsp: 2.771 ± 0.578
3.445IleGlu: 3.445 ± 0.49
1.273IlePhe: 1.273 ± 0.262
2.921IleGly: 2.921 ± 0.506
0.974IleHis: 0.974 ± 0.315
1.872IleIle: 1.872 ± 0.387
3.67IleLys: 3.67 ± 0.544
2.921IleLeu: 2.921 ± 0.413
1.123IleMet: 1.123 ± 0.369
1.723IleAsn: 1.723 ± 0.416
1.797IlePro: 1.797 ± 0.378
1.198IleGln: 1.198 ± 0.355
2.322IleArg: 2.322 ± 0.449
2.472IleSer: 2.472 ± 0.536
3.146IleThr: 3.146 ± 0.372
2.996IleVal: 2.996 ± 0.481
0.374IleTrp: 0.374 ± 0.155
1.797IleTyr: 1.797 ± 0.37
0.0IleXaa: 0.0 ± 0.0
Lys
5.767LysAla: 5.767 ± 0.676
0.374LysCys: 0.374 ± 0.173
4.194LysAsp: 4.194 ± 0.664
2.546LysGlu: 2.546 ± 0.405
1.723LysPhe: 1.723 ± 0.333
3.67LysGly: 3.67 ± 0.492
1.423LysHis: 1.423 ± 0.377
1.947LysIle: 1.947 ± 0.424
2.846LysLys: 2.846 ± 0.562
4.419LysLeu: 4.419 ± 0.671
1.797LysMet: 1.797 ± 0.387
2.696LysAsn: 2.696 ± 0.533
2.546LysPro: 2.546 ± 0.478
2.696LysGln: 2.696 ± 0.501
2.846LysArg: 2.846 ± 0.517
2.996LysSer: 2.996 ± 0.386
4.044LysThr: 4.044 ± 0.459
4.344LysVal: 4.344 ± 0.744
1.123LysTrp: 1.123 ± 0.275
2.247LysTyr: 2.247 ± 0.576
0.0LysXaa: 0.0 ± 0.0
Leu
6.516LeuAla: 6.516 ± 0.746
0.15LeuCys: 0.15 ± 0.128
4.044LeuAsp: 4.044 ± 0.604
4.194LeuGlu: 4.194 ± 0.45
2.696LeuPhe: 2.696 ± 0.418
6.591LeuGly: 6.591 ± 0.673
0.749LeuHis: 0.749 ± 0.276
3.445LeuIle: 3.445 ± 0.541
4.119LeuLys: 4.119 ± 0.713
3.595LeuLeu: 3.595 ± 0.389
1.797LeuMet: 1.797 ± 0.352
2.322LeuAsn: 2.322 ± 0.467
3.071LeuPro: 3.071 ± 0.439
3.895LeuGln: 3.895 ± 0.568
4.419LeuArg: 4.419 ± 0.559
4.643LeuSer: 4.643 ± 0.514
4.569LeuThr: 4.569 ± 0.492
5.243LeuVal: 5.243 ± 0.7
1.049LeuTrp: 1.049 ± 0.339
1.797LeuTyr: 1.797 ± 0.34
0.0LeuXaa: 0.0 ± 0.0
Met
4.569MetAla: 4.569 ± 0.635
0.3MetCys: 0.3 ± 0.159
2.097MetAsp: 2.097 ± 0.52
1.648MetGlu: 1.648 ± 0.402
0.824MetPhe: 0.824 ± 0.222
2.696MetGly: 2.696 ± 0.554
0.225MetHis: 0.225 ± 0.124
0.974MetIle: 0.974 ± 0.267
1.423MetLys: 1.423 ± 0.437
1.273MetLeu: 1.273 ± 0.367
0.524MetMet: 0.524 ± 0.241
1.273MetAsn: 1.273 ± 0.29
1.049MetPro: 1.049 ± 0.36
1.198MetGln: 1.198 ± 0.297
1.797MetArg: 1.797 ± 0.379
2.472MetSer: 2.472 ± 0.373
1.723MetThr: 1.723 ± 0.412
1.498MetVal: 1.498 ± 0.292
0.225MetTrp: 0.225 ± 0.111
0.599MetTyr: 0.599 ± 0.191
0.0MetXaa: 0.0 ± 0.0
Asn
3.895AsnAla: 3.895 ± 0.563
0.374AsnCys: 0.374 ± 0.162
1.573AsnAsp: 1.573 ± 0.429
2.621AsnGlu: 2.621 ± 0.432
1.348AsnPhe: 1.348 ± 0.322
3.37AsnGly: 3.37 ± 0.614
0.599AsnHis: 0.599 ± 0.179
1.198AsnIle: 1.198 ± 0.359
2.022AsnLys: 2.022 ± 0.351
2.696AsnLeu: 2.696 ± 0.422
0.899AsnMet: 0.899 ± 0.301
1.498AsnAsn: 1.498 ± 0.328
2.846AsnPro: 2.846 ± 0.412
1.498AsnGln: 1.498 ± 0.32
1.723AsnArg: 1.723 ± 0.397
2.996AsnSer: 2.996 ± 0.524
2.621AsnThr: 2.621 ± 0.439
2.771AsnVal: 2.771 ± 0.501
0.974AsnTrp: 0.974 ± 0.27
1.723AsnTyr: 1.723 ± 0.349
0.0AsnXaa: 0.0 ± 0.0
Pro
4.419ProAla: 4.419 ± 0.671
0.524ProCys: 0.524 ± 0.247
2.696ProAsp: 2.696 ± 0.438
2.996ProGlu: 2.996 ± 0.5
1.872ProPhe: 1.872 ± 0.389
4.119ProGly: 4.119 ± 0.685
0.599ProHis: 0.599 ± 0.191
1.573ProIle: 1.573 ± 0.305
2.097ProLys: 2.097 ± 0.423
2.696ProLeu: 2.696 ± 0.447
1.198ProMet: 1.198 ± 0.315
1.947ProAsn: 1.947 ± 0.358
1.273ProPro: 1.273 ± 0.393
1.498ProGln: 1.498 ± 0.321
2.247ProArg: 2.247 ± 0.483
2.172ProSer: 2.172 ± 0.332
3.295ProThr: 3.295 ± 0.665
3.22ProVal: 3.22 ± 0.426
0.674ProTrp: 0.674 ± 0.224
1.498ProTyr: 1.498 ± 0.314
0.0ProXaa: 0.0 ± 0.0
Gln
5.992GlnAla: 5.992 ± 0.771
0.225GlnCys: 0.225 ± 0.195
3.22GlnAsp: 3.22 ± 0.48
1.947GlnGlu: 1.947 ± 0.424
1.423GlnPhe: 1.423 ± 0.3
3.295GlnGly: 3.295 ± 0.469
0.599GlnHis: 0.599 ± 0.265
2.172GlnIle: 2.172 ± 0.363
2.172GlnLys: 2.172 ± 0.373
3.82GlnLeu: 3.82 ± 0.661
1.573GlnMet: 1.573 ± 0.295
1.423GlnAsn: 1.423 ± 0.408
1.723GlnPro: 1.723 ± 0.439
2.322GlnGln: 2.322 ± 0.489
3.22GlnArg: 3.22 ± 0.718
3.67GlnSer: 3.67 ± 0.515
2.771GlnThr: 2.771 ± 0.396
3.071GlnVal: 3.071 ± 0.421
0.674GlnTrp: 0.674 ± 0.212
1.648GlnTyr: 1.648 ± 0.367
0.0GlnXaa: 0.0 ± 0.0
Arg
5.093ArgAla: 5.093 ± 0.483
0.599ArgCys: 0.599 ± 0.229
3.895ArgAsp: 3.895 ± 0.505
2.996ArgGlu: 2.996 ± 0.489
1.348ArgPhe: 1.348 ± 0.31
4.119ArgGly: 4.119 ± 0.471
0.599ArgHis: 0.599 ± 0.23
2.172ArgIle: 2.172 ± 0.412
2.771ArgLys: 2.771 ± 0.464
3.37ArgLeu: 3.37 ± 0.552
1.348ArgMet: 1.348 ± 0.28
1.872ArgAsn: 1.872 ± 0.408
1.198ArgPro: 1.198 ± 0.258
2.621ArgGln: 2.621 ± 0.51
2.397ArgArg: 2.397 ± 0.481
3.071ArgSer: 3.071 ± 0.538
3.146ArgThr: 3.146 ± 0.496
4.718ArgVal: 4.718 ± 0.541
0.599ArgTrp: 0.599 ± 0.193
1.498ArgTyr: 1.498 ± 0.365
0.0ArgXaa: 0.0 ± 0.0
Ser
6.441SerAla: 6.441 ± 0.719
0.449SerCys: 0.449 ± 0.216
4.269SerAsp: 4.269 ± 0.618
2.996SerGlu: 2.996 ± 0.492
2.247SerPhe: 2.247 ± 0.438
6.89SerGly: 6.89 ± 1.067
1.123SerHis: 1.123 ± 0.295
2.846SerIle: 2.846 ± 0.517
2.696SerLys: 2.696 ± 0.46
4.269SerLeu: 4.269 ± 0.501
2.097SerMet: 2.097 ± 0.467
2.472SerAsn: 2.472 ± 0.431
2.621SerPro: 2.621 ± 0.357
2.621SerGln: 2.621 ± 0.36
2.771SerArg: 2.771 ± 0.424
4.718SerSer: 4.718 ± 0.915
4.194SerThr: 4.194 ± 0.624
4.419SerVal: 4.419 ± 0.61
1.423SerTrp: 1.423 ± 0.3
2.322SerTyr: 2.322 ± 0.386
0.0SerXaa: 0.0 ± 0.0
Thr
6.067ThrAla: 6.067 ± 0.824
0.674ThrCys: 0.674 ± 0.245
3.445ThrAsp: 3.445 ± 0.514
3.295ThrGlu: 3.295 ± 0.456
2.621ThrPhe: 2.621 ± 0.404
7.639ThrGly: 7.639 ± 0.975
0.824ThrHis: 0.824 ± 0.311
3.146ThrIle: 3.146 ± 0.612
4.419ThrLys: 4.419 ± 0.675
4.868ThrLeu: 4.868 ± 0.691
1.947ThrMet: 1.947 ± 0.37
2.472ThrAsn: 2.472 ± 0.335
4.419ThrPro: 4.419 ± 0.601
3.295ThrGln: 3.295 ± 0.608
1.648ThrArg: 1.648 ± 0.306
3.37ThrSer: 3.37 ± 0.648
4.943ThrThr: 4.943 ± 0.777
4.344ThrVal: 4.344 ± 0.661
1.498ThrTrp: 1.498 ± 0.264
2.546ThrTyr: 2.546 ± 0.5
0.0ThrXaa: 0.0 ± 0.0
Val
5.917ValAla: 5.917 ± 0.813
0.449ValCys: 0.449 ± 0.208
4.868ValAsp: 4.868 ± 0.547
3.071ValGlu: 3.071 ± 0.537
2.097ValPhe: 2.097 ± 0.367
5.542ValGly: 5.542 ± 0.727
1.723ValHis: 1.723 ± 0.299
2.472ValIle: 2.472 ± 0.537
4.868ValLys: 4.868 ± 0.639
5.168ValLeu: 5.168 ± 0.503
2.546ValMet: 2.546 ± 0.464
2.996ValAsn: 2.996 ± 0.485
3.146ValPro: 3.146 ± 0.506
3.295ValGln: 3.295 ± 0.709
3.82ValArg: 3.82 ± 0.545
5.467ValSer: 5.467 ± 0.747
6.366ValThr: 6.366 ± 0.674
3.82ValVal: 3.82 ± 0.726
0.974ValTrp: 0.974 ± 0.238
2.546ValTyr: 2.546 ± 0.404
0.0ValXaa: 0.0 ± 0.0
Trp
1.348TrpAla: 1.348 ± 0.261
0.225TrpCys: 0.225 ± 0.124
1.123TrpAsp: 1.123 ± 0.259
0.974TrpGlu: 0.974 ± 0.232
0.749TrpPhe: 0.749 ± 0.261
1.123TrpGly: 1.123 ± 0.28
0.0TrpHis: 0.0 ± 0.0
0.524TrpIle: 0.524 ± 0.211
1.123TrpLys: 1.123 ± 0.305
0.749TrpLeu: 0.749 ± 0.224
0.15TrpMet: 0.15 ± 0.089
0.524TrpAsn: 0.524 ± 0.189
0.674TrpPro: 0.674 ± 0.265
0.899TrpGln: 0.899 ± 0.203
0.824TrpArg: 0.824 ± 0.267
1.723TrpSer: 1.723 ± 0.356
1.797TrpThr: 1.797 ± 0.431
1.723TrpVal: 1.723 ± 0.277
0.374TrpTrp: 0.374 ± 0.157
0.599TrpTyr: 0.599 ± 0.211
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.445TyrAla: 3.445 ± 0.534
0.225TyrCys: 0.225 ± 0.137
2.022TyrAsp: 2.022 ± 0.411
2.172TyrGlu: 2.172 ± 0.449
1.123TyrPhe: 1.123 ± 0.295
3.295TyrGly: 3.295 ± 0.47
0.374TyrHis: 0.374 ± 0.178
1.348TyrIle: 1.348 ± 0.324
2.097TyrLys: 2.097 ± 0.37
2.696TyrLeu: 2.696 ± 0.354
0.974TyrMet: 0.974 ± 0.298
1.648TyrAsn: 1.648 ± 0.375
1.198TyrPro: 1.198 ± 0.316
2.397TyrGln: 2.397 ± 0.357
1.423TyrArg: 1.423 ± 0.349
2.322TyrSer: 2.322 ± 0.391
2.771TyrThr: 2.771 ± 0.496
2.696TyrVal: 2.696 ± 0.547
0.524TyrTrp: 0.524 ± 0.22
1.423TyrTyr: 1.423 ± 0.315
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (13353 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski