Flavobacteriaceae bacterium YIM 102668

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; unclassified Flavobacteriaceae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2667 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3L9M067|A0A3L9M067_9FLAO DUF262 domain-containing protein OS=Flavobacteriaceae bacterium YIM 102668 OX=2479857 GN=EAH69_13805 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.38KK3 pKa = 8.47ITLSLFAAMATSFAAAQVDD22 pKa = 4.05QLSNPDD28 pKa = 3.27VSGGNNNYY36 pKa = 10.27SSAEE40 pKa = 3.82QYY42 pKa = 11.33GDD44 pKa = 3.51RR45 pKa = 11.84NQISVLQEE53 pKa = 4.23GNDD56 pKa = 3.55NTSDD60 pKa = 3.2ILQDD64 pKa = 3.45GNRR67 pKa = 11.84NMNTTVSTGNSNDD80 pKa = 3.46SKK82 pKa = 11.47VEE84 pKa = 3.69QFGNSNVGNQTQTGDD99 pKa = 3.69LNSARR104 pKa = 11.84LLQEE108 pKa = 3.89GLSNRR113 pKa = 11.84SEE115 pKa = 4.0LVQTGNGNGSEE126 pKa = 4.09SFQYY130 pKa = 9.52GTLNRR135 pKa = 11.84VTTTQTGNEE144 pKa = 3.97NYY146 pKa = 7.96ATNYY150 pKa = 8.5QDD152 pKa = 4.81GVINSIAQTQNGNNNFIGAGQFGVGNSATQSQDD185 pKa = 2.73GNSNYY190 pKa = 10.77AGTQQDD196 pKa = 3.46GNANLVLQTQVGDD209 pKa = 3.79YY210 pKa = 10.87NEE212 pKa = 4.64ADD214 pKa = 3.44AAQEE218 pKa = 4.18GSGNLSVQTQALVANTANSFQDD240 pKa = 3.46GNFNEE245 pKa = 4.97SYY247 pKa = 8.9QTQTGFGQSALVEE260 pKa = 3.95QDD262 pKa = 2.92GNRR265 pKa = 11.84NIATQDD271 pKa = 3.01QLGYY275 pKa = 10.32FNEE278 pKa = 4.37AVASQTGNRR287 pKa = 11.84NEE289 pKa = 4.03SSQTQEE295 pKa = 3.03GWANEE300 pKa = 4.18AYY302 pKa = 10.71VMQDD306 pKa = 2.52GDD308 pKa = 3.91RR309 pKa = 11.84NNAVQSQNGLLNYY322 pKa = 9.94AISEE326 pKa = 4.11QTGNRR331 pKa = 11.84NEE333 pKa = 4.13SNQFQEE339 pKa = 4.44GLGNGAAIVQDD350 pKa = 3.86GNRR353 pKa = 11.84NSASQLQIGDD363 pKa = 3.71GNGAEE368 pKa = 4.46VYY370 pKa = 11.02QMGNEE375 pKa = 3.88NLASQIQLGDD385 pKa = 3.28GNYY388 pKa = 11.16ALIEE392 pKa = 3.94QAGDD396 pKa = 3.62GNSVFQGQGGDD407 pKa = 3.38NNIAYY412 pKa = 9.98SSQGGDD418 pKa = 3.53EE419 pKa = 4.36NSSSQLQIGDD429 pKa = 3.67GNIALGLQIGDD440 pKa = 3.42NNEE443 pKa = 3.98FVQNQYY449 pKa = 10.82GFEE452 pKa = 4.06NVAQTVQFGVDD463 pKa = 3.11NYY465 pKa = 11.78AHH467 pKa = 5.65VTQDD471 pKa = 3.05GSSNMADD478 pKa = 3.17QYY480 pKa = 11.58QEE482 pKa = 3.93GVNNVAAIEE491 pKa = 4.0QIGNGNSATQTQDD504 pKa = 3.05DD505 pKa = 4.75FGRR508 pKa = 11.84APNWNNVGGNDD519 pKa = 2.82NYY521 pKa = 11.49AYY523 pKa = 10.47VRR525 pKa = 11.84QEE527 pKa = 3.31GDD529 pKa = 3.58GNVSIQGQNGSNNEE543 pKa = 3.75IASLQRR549 pKa = 11.84GDD551 pKa = 3.47ANYY554 pKa = 10.47VEE556 pKa = 5.15TYY558 pKa = 10.63QDD560 pKa = 3.16GFDD563 pKa = 3.52NFGVSVQLGNNNEE576 pKa = 3.7IRR578 pKa = 11.84MGQHH582 pKa = 6.44GDD584 pKa = 3.22SNMADD589 pKa = 2.95TWQEE593 pKa = 3.95GNGNIANSTQIGLEE607 pKa = 3.68NYY609 pKa = 7.66TFNTQIGNEE618 pKa = 4.16NTLTTYY624 pKa = 11.0QNGAANQSYY633 pKa = 10.21IIQDD637 pKa = 3.48GNRR640 pKa = 11.84NNAEE644 pKa = 3.95VIQRR648 pKa = 11.84GRR650 pKa = 3.22

Molecular weight: 69.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3L9MGR1|A0A3L9MGR1_9FLAO Uncharacterized protein OS=Flavobacteriaceae bacterium YIM 102668 OX=2479857 GN=EAH69_08200 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNRR11 pKa = 11.84KK12 pKa = 8.86KK13 pKa = 10.03RR14 pKa = 11.84NKK16 pKa = 9.59HH17 pKa = 4.05GFRR20 pKa = 11.84EE21 pKa = 4.13RR22 pKa = 11.84MASKK26 pKa = 10.63NGRR29 pKa = 11.84KK30 pKa = 9.21LLAARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.53KK38 pKa = 10.12GRR40 pKa = 11.84KK41 pKa = 8.55ALTVSSVRR49 pKa = 11.84TKK51 pKa = 10.73RR52 pKa = 3.29

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2667

0

2667

857452

32

2398

321.5

36.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.976 ± 0.051

0.667 ± 0.014

5.596 ± 0.033

6.922 ± 0.049

5.426 ± 0.039

6.07 ± 0.044

1.642 ± 0.02

8.566 ± 0.052

8.026 ± 0.045

8.995 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.183 ± 0.022

6.808 ± 0.063

3.163 ± 0.025

3.663 ± 0.029

3.206 ± 0.033

6.142 ± 0.038

5.525 ± 0.04

6.141 ± 0.039

0.963 ± 0.017

4.32 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski