Odonata-associated circular virus-9
Average proteome isoelectric point is 8.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UI03|A0A0B4UI03_9VIRU Putative capsid protein OS=Odonata-associated circular virus-9 OX=1592129 PE=4 SV=1
MM1 pKa = 7.45 IFKK4 pKa = 10.58 KK5 pKa = 10.38 KK6 pKa = 10.05 IYY8 pKa = 9.89 FSNKK12 pKa = 7.93 IKK14 pKa = 10.17 MSSRR18 pKa = 11.84 NYY20 pKa = 10.38 VLTIYY25 pKa = 10.64 DD26 pKa = 3.84 FNLLPKK32 pKa = 10.1 LQDD35 pKa = 3.15 SAIRR39 pKa = 11.84 YY40 pKa = 7.69 SAYY43 pKa = 9.47 TDD45 pKa = 4.3 EE46 pKa = 4.82 ICPTTGRR53 pKa = 11.84 PHH55 pKa = 5.34 KK56 pKa = 9.79 QAYY59 pKa = 7.93 IVLYY63 pKa = 10.0 KK64 pKa = 10.06 KK65 pKa = 10.18 QRR67 pKa = 11.84 PSFVRR72 pKa = 11.84 KK73 pKa = 10.04 LFPNTYY79 pKa = 7.99 NHH81 pKa = 6.38 IMNGTLEE88 pKa = 4.02 QNDD91 pKa = 3.49 IYY93 pKa = 10.67 ISKK96 pKa = 10.76 KK97 pKa = 7.94 NTQQIHH103 pKa = 6.18 EE104 pKa = 4.53 YY105 pKa = 9.69 GDD107 pKa = 3.34 KK108 pKa = 11.1 PMTQEE113 pKa = 3.82 EE114 pKa = 4.46 KK115 pKa = 10.91 GEE117 pKa = 3.88 EE118 pKa = 4.09 GAAYY122 pKa = 9.0 WRR124 pKa = 11.84 EE125 pKa = 3.83 QLEE128 pKa = 4.25 LAKK131 pKa = 10.04 KK132 pKa = 10.41 DD133 pKa = 4.09 PSLCDD138 pKa = 3.35 PKK140 pKa = 11.02 LQIIHH145 pKa = 6.39 HH146 pKa = 6.67 RR147 pKa = 11.84 NLEE150 pKa = 4.48 AIHH153 pKa = 6.31 EE154 pKa = 4.2 KK155 pKa = 10.49 AKK157 pKa = 10.36 RR158 pKa = 11.84 VKK160 pKa = 10.78 LSDD163 pKa = 4.73 LDD165 pKa = 5.2 KK166 pKa = 11.35 LDD168 pKa = 3.24 NWWYY172 pKa = 10.0 IGCTGTGKK180 pKa = 10.36 SRR182 pKa = 11.84 TARR185 pKa = 11.84 QLYY188 pKa = 7.94 PDD190 pKa = 5.05 AYY192 pKa = 9.77 IKK194 pKa = 10.4 QCNKK198 pKa = 7.01 WWNDD202 pKa = 3.04 YY203 pKa = 11.11 DD204 pKa = 4.3 GEE206 pKa = 4.35 EE207 pKa = 4.07 TVIIDD212 pKa = 5.38 DD213 pKa = 4.99 LNPDD217 pKa = 3.93 HH218 pKa = 7.18 KK219 pKa = 11.29 FMANFIDD226 pKa = 3.86 QWCDD230 pKa = 2.95 HH231 pKa = 6.49 YY232 pKa = 11.34 PFPAEE237 pKa = 3.92 FKK239 pKa = 10.57 GGQKK243 pKa = 9.58 TIRR246 pKa = 11.84 PKK248 pKa = 10.71 RR249 pKa = 11.84 FIITTRR255 pKa = 11.84 YY256 pKa = 7.83 TIEE259 pKa = 4.63 EE260 pKa = 3.89 ILGEE264 pKa = 4.2 NNFSQIEE271 pKa = 4.05 SWKK274 pKa = 10.3 RR275 pKa = 11.84 RR276 pKa = 11.84 FQIKK280 pKa = 9.79 EE281 pKa = 3.73 FF282 pKa = 4.02
Molecular weight: 33.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.345
IPC2_protein 8.317
IPC_protein 8.214
Toseland 8.741
ProMoST 8.712
Dawson 9.107
Bjellqvist 9.019
Wikipedia 9.253
Rodwell 9.268
Grimsley 9.077
Solomon 9.194
Lehninger 9.151
Nozaki 9.107
DTASelect 8.916
Thurlkill 9.019
EMBOSS 9.253
Sillero 9.194
Patrickios 4.431
IPC_peptide 9.18
IPC2_peptide 7.688
IPC2.peptide.svr19 7.651
Protein with the highest isoelectric point:
>tr|A0A0B4UI03|A0A0B4UI03_9VIRU Putative capsid protein OS=Odonata-associated circular virus-9 OX=1592129 PE=4 SV=1
MM1 pKa = 7.66 ARR3 pKa = 11.84 RR4 pKa = 11.84 KK5 pKa = 9.53 YY6 pKa = 10.48 FRR8 pKa = 11.84 RR9 pKa = 11.84 NPYY12 pKa = 8.93 RR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 FKK17 pKa = 10.32 RR18 pKa = 11.84 RR19 pKa = 11.84 NYY21 pKa = 9.25 RR22 pKa = 11.84 FSSRR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 SGFRR32 pKa = 11.84 RR33 pKa = 11.84 WRR35 pKa = 11.84 VARR38 pKa = 11.84 QKK40 pKa = 11.33 NLVFSNSQLVTLRR53 pKa = 11.84 YY54 pKa = 9.57 CKK56 pKa = 10.47 NIQLNPANGATISVATFRR74 pKa = 11.84 ANDD77 pKa = 3.13 IYY79 pKa = 11.12 FCDD82 pKa = 4.0 GLAGSEE88 pKa = 4.06 PKK90 pKa = 9.91 GTTEE94 pKa = 3.55 WEE96 pKa = 4.16 GFYY99 pKa = 11.04 NHH101 pKa = 6.45 YY102 pKa = 10.7 LVIGSKK108 pKa = 7.14 MTAMFNSGSSTTSNGAGTVGVVLTDD133 pKa = 3.63 TSNVAYY139 pKa = 9.92 INNTDD144 pKa = 3.25 VMTEE148 pKa = 3.56 PRR150 pKa = 11.84 ARR152 pKa = 11.84 WKK154 pKa = 10.59 IIGNQTGTGTKK165 pKa = 9.53 TITKK169 pKa = 9.56 GYY171 pKa = 8.75 SPKK174 pKa = 10.66 KK175 pKa = 10.06 FFTIHH180 pKa = 6.5 NLLDD184 pKa = 3.79 NQEE187 pKa = 4.52 HH188 pKa = 6.13 YY189 pKa = 10.24 GAPVSVSPPEE199 pKa = 3.54 IAFFQVFFAPHH210 pKa = 6.22 AASAGTGGPWVTVIIDD226 pKa = 3.73 YY227 pKa = 10.92 KK228 pKa = 11.31 VMYY231 pKa = 8.56 TGPKK235 pKa = 9.62 RR236 pKa = 11.84 IAPSS240 pKa = 3.13
Molecular weight: 27.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 10.028
IPC_protein 10.774
Toseland 10.657
ProMoST 10.409
Dawson 10.804
Bjellqvist 10.526
Wikipedia 11.023
Rodwell 11.052
Grimsley 10.877
Solomon 10.891
Lehninger 10.847
Nozaki 10.643
DTASelect 10.526
Thurlkill 10.687
EMBOSS 11.067
Sillero 10.73
Patrickios 10.716
IPC_peptide 10.891
IPC2_peptide 9.502
IPC2.peptide.svr19 8.228
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
522
240
282
261.0
30.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.747 ± 1.189
1.341 ± 0.344
4.598 ± 1.423
4.789 ± 1.553
5.364 ± 0.601
6.13 ± 1.494
2.299 ± 0.429
7.471 ± 1.394
8.238 ± 1.913
5.172 ± 0.965
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.916 ± 0.114
6.322 ± 0.516
4.789 ± 0.143
4.023 ± 1.033
7.088 ± 1.41
5.747 ± 1.189
7.28 ± 1.28
4.023 ± 1.793
2.107 ± 0.299
5.556 ± 0.377
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here