Collinsella sp. An307

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Coriobacteriales; Coriobacteriaceae; Collinsella; unclassified Collinsella

Average proteome isoelectric point is 5.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1893 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4AJA6|A0A1Y4AJA6_9ACTN Single-stranded-DNA-specific exonuclease RecJ OS=Collinsella sp. An307 OX=1965630 GN=B5F89_00375 PE=3 SV=1
MM1 pKa = 7.82KK2 pKa = 10.25DD3 pKa = 3.3LLVSRR8 pKa = 11.84RR9 pKa = 11.84SIAGFGVAAAAVALTACGNTAAEE32 pKa = 4.82GSAPAAGSASAEE44 pKa = 4.16SSDD47 pKa = 4.53GSVQLQVFAANSLEE61 pKa = 3.91KK62 pKa = 10.88AMPEE66 pKa = 3.88VEE68 pKa = 4.4ALYY71 pKa = 10.19TEE73 pKa = 4.24QTGVTFADD81 pKa = 3.64TQYY84 pKa = 11.05KK85 pKa = 10.56GSGDD89 pKa = 3.46LVEE92 pKa = 4.46QMRR95 pKa = 11.84GGAPVDD101 pKa = 3.36ILITASAGTMDD112 pKa = 4.44DD113 pKa = 3.73AEE115 pKa = 4.28EE116 pKa = 4.57AGLVDD121 pKa = 4.04ADD123 pKa = 3.64TRR125 pKa = 11.84EE126 pKa = 4.01DD127 pKa = 3.58MFVNDD132 pKa = 3.84LVIVRR137 pKa = 11.84AEE139 pKa = 4.05GSDD142 pKa = 3.5IEE144 pKa = 4.34ISSLKK149 pKa = 10.68DD150 pKa = 3.2VASVDD155 pKa = 3.47GNIAIGEE162 pKa = 4.22PGAVPAGKK170 pKa = 9.9YY171 pKa = 10.32ANQALASVGLYY182 pKa = 9.85TEE184 pKa = 5.27DD185 pKa = 3.29EE186 pKa = 4.8GEE188 pKa = 4.22GGEE191 pKa = 4.19YY192 pKa = 10.27DD193 pKa = 4.53ASIADD198 pKa = 4.03KK199 pKa = 10.83VVQADD204 pKa = 3.75KK205 pKa = 11.36VGTAAQYY212 pKa = 11.29VSTGDD217 pKa = 3.68CVIGFVYY224 pKa = 10.44TSDD227 pKa = 3.09VYY229 pKa = 10.75RR230 pKa = 11.84YY231 pKa = 10.44DD232 pKa = 4.21GIEE235 pKa = 3.86VAYY238 pKa = 7.82TCPAEE243 pKa = 3.97SHH245 pKa = 6.2KK246 pKa = 11.19AIVYY250 pKa = 8.37PGAVATSSEE259 pKa = 4.24NADD262 pKa = 3.34AAADD266 pKa = 4.02FLAFCMDD273 pKa = 3.91NEE275 pKa = 4.33DD276 pKa = 5.44ALAIWAEE283 pKa = 4.15YY284 pKa = 10.49GFEE287 pKa = 3.98LAA289 pKa = 5.71

Molecular weight: 29.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4ABJ6|A0A1Y4ABJ6_9ACTN Glycerol-3-phosphate dehydrogenase [NAD(P)+] OS=Collinsella sp. An307 OX=1965630 GN=gpsA PE=3 SV=1
MM1 pKa = 7.92PPRR4 pKa = 11.84PSLPRR9 pKa = 11.84SAPSTRR15 pKa = 11.84RR16 pKa = 11.84SRR18 pKa = 11.84PSSSRR23 pKa = 11.84RR24 pKa = 11.84APSSRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84PRR34 pKa = 11.84SLIRR38 pKa = 11.84RR39 pKa = 11.84PSLSLRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84FSLRR51 pKa = 11.84TCPPTTRR58 pKa = 11.84RR59 pKa = 11.84GASALLTSSLARR71 pKa = 11.84RR72 pKa = 11.84TSPTSSRR79 pKa = 11.84ASSTT83 pKa = 3.3

Molecular weight: 9.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1893

0

1893

640865

37

2761

338.5

36.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.378 ± 0.079

1.451 ± 0.022

6.281 ± 0.048

6.876 ± 0.06

3.588 ± 0.04

8.141 ± 0.049

1.987 ± 0.024

5.178 ± 0.043

3.12 ± 0.043

9.258 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.537 ± 0.03

2.666 ± 0.026

4.428 ± 0.034

2.636 ± 0.027

6.597 ± 0.062

5.479 ± 0.05

5.578 ± 0.046

7.975 ± 0.042

1.058 ± 0.021

2.788 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski