Lake Sarah-associated circular molecule 4
Average proteome isoelectric point is 9.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A126G9Y1|A0A126G9Y1_9VIRU Uncharacterized protein OS=Lake Sarah-associated circular molecule 4 OX=1685729 PE=4 SV=1
MM1 pKa = 7.63 VLEE4 pKa = 4.21 YY5 pKa = 10.33 HH6 pKa = 6.17 RR7 pKa = 11.84 QAGLVRR13 pKa = 11.84 SQRR16 pKa = 11.84 RR17 pKa = 11.84 KK18 pKa = 10.22 LVWATTDD25 pKa = 3.16 QSVNIPAGQISNTNLAATLSVAGASLLGTTIMRR58 pKa = 11.84 THH60 pKa = 6.67 LRR62 pKa = 11.84 IEE64 pKa = 4.08 MTSIAGQATTDD75 pKa = 3.2 QYY77 pKa = 11.36 RR78 pKa = 11.84 IGLIVGRR85 pKa = 11.84 TSDD88 pKa = 3.23 VGINVAGQQDD98 pKa = 3.97 PSNPEE103 pKa = 4.13 LDD105 pKa = 3.45 WVHH108 pKa = 7.15 LDD110 pKa = 3.54 RR111 pKa = 11.84 VIPTASGATVDD122 pKa = 4.31 STQHH126 pKa = 4.93 WVLDD130 pKa = 3.76 NRR132 pKa = 11.84 SKK134 pKa = 11.38 RR135 pKa = 11.84 KK136 pKa = 7.96 MQEE139 pKa = 3.45 LNQAYY144 pKa = 9.71 LLCLQQNAAAARR156 pKa = 11.84 TFNIFARR163 pKa = 11.84 VLFALPP169 pKa = 3.59
Molecular weight: 18.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.208
IPC2_protein 9.077
IPC_protein 9.458
Toseland 9.677
ProMoST 9.545
Dawson 9.955
Bjellqvist 9.721
Wikipedia 10.189
Rodwell 10.058
Grimsley 10.058
Solomon 10.058
Lehninger 10.028
Nozaki 9.75
DTASelect 9.706
Thurlkill 9.78
EMBOSS 10.101
Sillero 9.897
Patrickios 7.644
IPC_peptide 10.043
IPC2_peptide 8.668
IPC2.peptide.svr19 8.038
Protein with the highest isoelectric point:
>tr|A0A126G9Y1|A0A126G9Y1_9VIRU Uncharacterized protein OS=Lake Sarah-associated circular molecule 4 OX=1685729 PE=4 SV=1
MM1 pKa = 7.63 VLEE4 pKa = 4.21 YY5 pKa = 10.33 HH6 pKa = 6.17 RR7 pKa = 11.84 QAGLVRR13 pKa = 11.84 SQRR16 pKa = 11.84 RR17 pKa = 11.84 KK18 pKa = 10.22 LVWATTDD25 pKa = 3.16 QSVNIPAGQISNTNLAATLSVAGASLLGTTIMRR58 pKa = 11.84 THH60 pKa = 6.67 LRR62 pKa = 11.84 IEE64 pKa = 4.08 MTSIAGQATTDD75 pKa = 3.2 QYY77 pKa = 11.36 RR78 pKa = 11.84 IGLIVGRR85 pKa = 11.84 TSDD88 pKa = 3.23 VGINVAGQQDD98 pKa = 3.97 PSNPEE103 pKa = 4.13 LDD105 pKa = 3.45 WVHH108 pKa = 7.15 LDD110 pKa = 3.54 RR111 pKa = 11.84 VIPTASGATVDD122 pKa = 4.31 STQHH126 pKa = 4.93 WVLDD130 pKa = 3.76 NRR132 pKa = 11.84 SKK134 pKa = 11.38 RR135 pKa = 11.84 KK136 pKa = 7.96 MQEE139 pKa = 3.45 LNQAYY144 pKa = 9.71 LLCLQQNAAAARR156 pKa = 11.84 TFNIFARR163 pKa = 11.84 VLFALPP169 pKa = 3.59
Molecular weight: 18.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.208
IPC2_protein 9.077
IPC_protein 9.458
Toseland 9.677
ProMoST 9.545
Dawson 9.955
Bjellqvist 9.721
Wikipedia 10.189
Rodwell 10.058
Grimsley 10.058
Solomon 10.058
Lehninger 10.028
Nozaki 9.75
DTASelect 9.706
Thurlkill 9.78
EMBOSS 10.101
Sillero 9.897
Patrickios 7.644
IPC_peptide 10.043
IPC2_peptide 8.668
IPC2.peptide.svr19 8.038
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
169
169
169
169.0
18.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.243 ± 0.0
0.592 ± 0.0
4.734 ± 0.0
2.367 ± 0.0
1.775 ± 0.0
5.917 ± 0.0
2.367 ± 0.0
5.917 ± 0.0
1.775 ± 0.0
10.651 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.367 ± 0.0
5.325 ± 0.0
2.959 ± 0.0
7.692 ± 0.0
7.692 ± 0.0
6.509 ± 0.0
8.876 ± 0.0
7.692 ± 0.0
1.775 ± 0.0
1.775 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here