Lysinibacillus telephonicus
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3959 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S0J0Z7|A0A3S0J0Z7_9BACI Uncharacterized protein OS=Lysinibacillus telephonicus OX=1714840 GN=EKG35_13430 PE=4 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.64 KK3 pKa = 10.43 LGTFLLSFSLLIALFPQITMAAQDD27 pKa = 3.73 TNFEE31 pKa = 3.99 QDD33 pKa = 3.91 LINYY37 pKa = 8.58 LEE39 pKa = 4.08 QVSNEE44 pKa = 3.82 RR45 pKa = 11.84 GFEE48 pKa = 4.05 VTKK51 pKa = 10.89 EE52 pKa = 3.99 DD53 pKa = 3.29 IALSLSYY60 pKa = 11.38 YY61 pKa = 10.33 EE62 pKa = 6.01 DD63 pKa = 3.18 SLEE66 pKa = 4.17 NYY68 pKa = 7.66 NTVEE72 pKa = 4.22 EE73 pKa = 4.4 LSQFLGEE80 pKa = 4.65 IIKK83 pKa = 10.49 ADD85 pKa = 3.73 YY86 pKa = 11.33 SNVDD90 pKa = 3.67 SIYY93 pKa = 10.15 EE94 pKa = 3.96 QYY96 pKa = 11.29 EE97 pKa = 4.01 LTEE100 pKa = 4.08 EE101 pKa = 4.63 SLVQLLKK108 pKa = 11.18 EE109 pKa = 4.16 NGEE112 pKa = 4.05 QLSDD116 pKa = 4.17 YY117 pKa = 11.03 IFLDD121 pKa = 4.08 DD122 pKa = 5.9 LDD124 pKa = 4.17 TAVYY128 pKa = 9.29 FYY130 pKa = 11.01 TEE132 pKa = 4.58 DD133 pKa = 3.45 GTFEE137 pKa = 4.43 RR138 pKa = 11.84 DD139 pKa = 3.19 PNFEE143 pKa = 4.14 QNLIDD148 pKa = 3.96 YY149 pKa = 9.52 LADD152 pKa = 3.35 VSDD155 pKa = 3.47 QRR157 pKa = 11.84 GYY159 pKa = 11.34 NVTRR163 pKa = 11.84 DD164 pKa = 3.53 NVVNSLAIYY173 pKa = 9.39 EE174 pKa = 4.47 SNLEE178 pKa = 4.02 DD179 pKa = 4.08 FEE181 pKa = 5.54 SVEE184 pKa = 3.99 QLSDD188 pKa = 3.43 FLGEE192 pKa = 4.39 VIQADD197 pKa = 4.56 YY198 pKa = 11.76 SNLDD202 pKa = 3.54 YY203 pKa = 10.84 FVEE206 pKa = 4.5 NYY208 pKa = 10.44 GLSQQEE214 pKa = 4.31 LFNLLEE220 pKa = 4.72 SNDD223 pKa = 3.88 EE224 pKa = 4.42 NINDD228 pKa = 4.07 YY229 pKa = 10.91 IYY231 pKa = 10.46 IDD233 pKa = 4.78 DD234 pKa = 5.47 LDD236 pKa = 3.89 NTVWKK241 pKa = 10.85 YY242 pKa = 10.94 VGNDD246 pKa = 3.25 LSVMFEE252 pKa = 4.28 YY253 pKa = 9.69 LTEE256 pKa = 4.71 DD257 pKa = 4.16 LLPLFQEE264 pKa = 4.05 IDD266 pKa = 3.53 LTDD269 pKa = 3.69 EE270 pKa = 3.7 EE271 pKa = 4.66 LKK273 pKa = 10.85 RR274 pKa = 11.84 FEE276 pKa = 3.99 NHH278 pKa = 7.06 LISLEE283 pKa = 4.04 DD284 pKa = 3.4 HH285 pKa = 6.77 FSNPEE290 pKa = 3.81 TLEE293 pKa = 4.06 LLEE296 pKa = 3.96 QLNYY300 pKa = 10.65 EE301 pKa = 4.77 LIALEE306 pKa = 4.49 SDD308 pKa = 3.76 MNSQPTVEE316 pKa = 4.01 QMEE319 pKa = 4.29 MSLSILEE326 pKa = 4.64 KK327 pKa = 10.34 IFSILQLKK335 pKa = 10.24 ASYY338 pKa = 11.07 SLVNDD343 pKa = 5.27 AIEE346 pKa = 4.33 TPISLMDD353 pKa = 3.88 LMKK356 pKa = 10.24 MEE358 pKa = 4.29 VLEE361 pKa = 4.4 GTDD364 pKa = 3.6 LKK366 pKa = 11.33 VAFYY370 pKa = 10.78 SSEE373 pKa = 3.95 GQFLGDD379 pKa = 4.55 LIITEE384 pKa = 4.19 EE385 pKa = 4.57 FINSGLVTDD394 pKa = 5.03 LGEE397 pKa = 4.28 QIVDD401 pKa = 3.86 SAVEE405 pKa = 4.01 QPPVVQPKK413 pKa = 9.87 KK414 pKa = 10.52 QINTTQTLAEE424 pKa = 4.58 EE425 pKa = 4.33 YY426 pKa = 8.8 HH427 pKa = 5.2 TVKK430 pKa = 10.59 GGKK433 pKa = 9.0 LPKK436 pKa = 9.21 TASDD440 pKa = 4.27 YY441 pKa = 11.22 IPNALFGLFIVMLGIFMYY459 pKa = 10.58 GKK461 pKa = 10.2 VRR463 pKa = 11.84 TSS465 pKa = 2.77
Molecular weight: 53.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.757
IPC_protein 3.757
Toseland 3.554
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.605
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.668
Nozaki 3.834
DTASelect 3.999
Thurlkill 3.579
EMBOSS 3.63
Sillero 3.872
Patrickios 1.113
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.774
Protein with the highest isoelectric point:
>tr|A0A3S0I3B6|A0A3S0I3B6_9BACI HAMP domain-containing protein OS=Lysinibacillus telephonicus OX=1714840 GN=EKG35_04675 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.54 QPKK8 pKa = 9.44 KK9 pKa = 7.96 RR10 pKa = 11.84 KK11 pKa = 8.69 HH12 pKa = 5.94 SKK14 pKa = 8.54 VHH16 pKa = 5.68 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 10.19 NGRR28 pKa = 11.84 KK29 pKa = 8.91 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.81 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLSAA44 pKa = 4.05
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 11.067
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.027
Rodwell 12.398
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.135
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.988
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3959
0
3959
1178354
14
1912
297.6
33.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.84 ± 0.044
0.715 ± 0.011
4.941 ± 0.031
7.608 ± 0.046
4.659 ± 0.039
6.603 ± 0.037
1.911 ± 0.015
8.648 ± 0.041
6.83 ± 0.039
9.737 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.563 ± 0.016
4.952 ± 0.034
3.438 ± 0.026
3.71 ± 0.027
3.72 ± 0.032
6.132 ± 0.035
5.474 ± 0.032
6.928 ± 0.032
0.965 ± 0.013
3.626 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here