Amino acid dipepetide frequency for Curvibacter phage TJ1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.021AlaAla: 19.021 ± 2.011
1.463AlaCys: 1.463 ± 0.354
7.402AlaAsp: 7.402 ± 0.719
7.746AlaGlu: 7.746 ± 1.074
3.529AlaPhe: 3.529 ± 0.683
10.07AlaGly: 10.07 ± 1.112
1.377AlaHis: 1.377 ± 0.317
6.541AlaIle: 6.541 ± 0.554
6.455AlaLys: 6.455 ± 0.978
10.93AlaLeu: 10.93 ± 1.085
4.648AlaMet: 4.648 ± 0.692
3.184AlaAsn: 3.184 ± 0.567
4.648AlaPro: 4.648 ± 0.591
4.734AlaGln: 4.734 ± 0.713
7.23AlaArg: 7.23 ± 0.797
7.574AlaSer: 7.574 ± 0.808
6.369AlaThr: 6.369 ± 0.712
7.143AlaVal: 7.143 ± 0.609
1.721AlaTrp: 1.721 ± 0.322
3.271AlaTyr: 3.271 ± 0.457
0.0AlaXaa: 0.0 ± 0.0
Cys
0.602CysAla: 0.602 ± 0.244
0.172CysCys: 0.172 ± 0.113
0.775CysAsp: 0.775 ± 0.25
0.602CysGlu: 0.602 ± 0.184
0.172CysPhe: 0.172 ± 0.119
0.947CysGly: 0.947 ± 0.297
0.086CysHis: 0.086 ± 0.081
0.43CysIle: 0.43 ± 0.192
0.43CysLys: 0.43 ± 0.202
0.861CysLeu: 0.861 ± 0.239
0.0CysMet: 0.0 ± 0.0
0.258CysAsn: 0.258 ± 0.133
0.258CysPro: 0.258 ± 0.16
0.689CysGln: 0.689 ± 0.275
0.602CysArg: 0.602 ± 0.201
0.344CysSer: 0.344 ± 0.167
0.602CysThr: 0.602 ± 0.196
0.947CysVal: 0.947 ± 0.293
0.258CysTrp: 0.258 ± 0.144
0.43CysTyr: 0.43 ± 0.192
0.0CysXaa: 0.0 ± 0.0
Asp
7.918AspAla: 7.918 ± 0.986
0.602AspCys: 0.602 ± 0.211
3.787AspAsp: 3.787 ± 0.471
3.271AspGlu: 3.271 ± 0.617
2.238AspPhe: 2.238 ± 0.451
4.734AspGly: 4.734 ± 0.558
0.861AspHis: 0.861 ± 0.328
3.271AspIle: 3.271 ± 0.505
3.529AspLys: 3.529 ± 0.595
5.852AspLeu: 5.852 ± 0.801
1.377AspMet: 1.377 ± 0.416
1.205AspAsn: 1.205 ± 0.303
1.98AspPro: 1.98 ± 0.38
2.324AspGln: 2.324 ± 0.39
4.992AspArg: 4.992 ± 0.486
2.926AspSer: 2.926 ± 0.394
2.066AspThr: 2.066 ± 0.347
3.873AspVal: 3.873 ± 0.533
1.119AspTrp: 1.119 ± 0.372
1.635AspTyr: 1.635 ± 0.284
0.0AspXaa: 0.0 ± 0.0
Glu
6.799GluAla: 6.799 ± 0.866
0.775GluCys: 0.775 ± 0.278
2.152GluAsp: 2.152 ± 0.549
2.754GluGlu: 2.754 ± 0.427
2.238GluPhe: 2.238 ± 0.55
3.873GluGly: 3.873 ± 0.693
1.033GluHis: 1.033 ± 0.265
2.066GluIle: 2.066 ± 0.397
1.98GluLys: 1.98 ± 0.563
4.992GluLeu: 4.992 ± 0.582
1.549GluMet: 1.549 ± 0.311
1.291GluAsn: 1.291 ± 0.391
1.893GluPro: 1.893 ± 0.335
3.184GluGln: 3.184 ± 0.482
4.561GluArg: 4.561 ± 0.643
3.012GluSer: 3.012 ± 0.595
2.84GluThr: 2.84 ± 0.473
4.906GluVal: 4.906 ± 0.715
0.689GluTrp: 0.689 ± 0.248
1.377GluTyr: 1.377 ± 0.257
0.0GluXaa: 0.0 ± 0.0
Phe
3.357PheAla: 3.357 ± 0.525
0.344PheCys: 0.344 ± 0.171
2.668PheAsp: 2.668 ± 0.428
1.635PheGlu: 1.635 ± 0.354
1.377PhePhe: 1.377 ± 0.381
2.84PheGly: 2.84 ± 0.446
0.516PheHis: 0.516 ± 0.222
0.947PheIle: 0.947 ± 0.214
1.463PheLys: 1.463 ± 0.365
1.377PheLeu: 1.377 ± 0.381
0.775PheMet: 0.775 ± 0.276
1.205PheAsn: 1.205 ± 0.354
1.549PhePro: 1.549 ± 0.43
1.377PheGln: 1.377 ± 0.358
1.463PheArg: 1.463 ± 0.316
1.549PheSer: 1.549 ± 0.331
2.324PheThr: 2.324 ± 0.438
2.152PheVal: 2.152 ± 0.384
0.516PheTrp: 0.516 ± 0.182
1.119PheTyr: 1.119 ± 0.329
0.0PheXaa: 0.0 ± 0.0
Gly
8.779GlyAla: 8.779 ± 1.16
0.344GlyCys: 0.344 ± 0.146
4.561GlyAsp: 4.561 ± 0.567
4.217GlyGlu: 4.217 ± 0.458
2.754GlyPhe: 2.754 ± 0.571
6.799GlyGly: 6.799 ± 1.119
1.463GlyHis: 1.463 ± 0.436
2.324GlyIle: 2.324 ± 0.45
5.25GlyLys: 5.25 ± 0.605
7.23GlyLeu: 7.23 ± 0.896
2.496GlyMet: 2.496 ± 0.484
2.066GlyAsn: 2.066 ± 0.455
2.152GlyPro: 2.152 ± 0.416
3.615GlyGln: 3.615 ± 0.487
4.734GlyArg: 4.734 ± 0.613
5.766GlySer: 5.766 ± 0.925
6.025GlyThr: 6.025 ± 0.694
5.336GlyVal: 5.336 ± 0.568
2.238GlyTrp: 2.238 ± 0.482
2.926GlyTyr: 2.926 ± 0.422
0.0GlyXaa: 0.0 ± 0.0
His
1.549HisAla: 1.549 ± 0.423
0.43HisCys: 0.43 ± 0.201
1.291HisAsp: 1.291 ± 0.325
0.947HisGlu: 0.947 ± 0.28
0.43HisPhe: 0.43 ± 0.177
1.549HisGly: 1.549 ± 0.325
0.43HisHis: 0.43 ± 0.16
0.516HisIle: 0.516 ± 0.205
0.516HisLys: 0.516 ± 0.183
1.205HisLeu: 1.205 ± 0.317
0.861HisMet: 0.861 ± 0.249
0.602HisAsn: 0.602 ± 0.197
1.119HisPro: 1.119 ± 0.276
0.861HisGln: 0.861 ± 0.263
1.033HisArg: 1.033 ± 0.315
0.775HisSer: 0.775 ± 0.237
0.775HisThr: 0.775 ± 0.246
1.033HisVal: 1.033 ± 0.221
0.086HisTrp: 0.086 ± 0.088
0.689HisTyr: 0.689 ± 0.23
0.0HisXaa: 0.0 ± 0.0
Ile
4.906IleAla: 4.906 ± 0.511
0.43IleCys: 0.43 ± 0.168
2.926IleAsp: 2.926 ± 0.613
3.615IleGlu: 3.615 ± 0.6
1.033IlePhe: 1.033 ± 0.27
3.787IleGly: 3.787 ± 0.567
0.516IleHis: 0.516 ± 0.247
1.377IleIle: 1.377 ± 0.32
2.668IleLys: 2.668 ± 0.407
1.893IleLeu: 1.893 ± 0.347
0.775IleMet: 0.775 ± 0.271
2.152IleAsn: 2.152 ± 0.362
2.066IlePro: 2.066 ± 0.398
1.807IleGln: 1.807 ± 0.387
2.754IleArg: 2.754 ± 0.352
2.152IleSer: 2.152 ± 0.381
3.529IleThr: 3.529 ± 0.648
2.066IleVal: 2.066 ± 0.35
0.258IleTrp: 0.258 ± 0.152
0.775IleTyr: 0.775 ± 0.28
0.0IleXaa: 0.0 ± 0.0
Lys
6.627LysAla: 6.627 ± 0.932
0.258LysCys: 0.258 ± 0.162
2.582LysAsp: 2.582 ± 0.388
2.066LysGlu: 2.066 ± 0.425
1.463LysPhe: 1.463 ± 0.398
4.561LysGly: 4.561 ± 0.652
1.033LysHis: 1.033 ± 0.355
1.635LysIle: 1.635 ± 0.405
1.98LysLys: 1.98 ± 0.469
5.594LysLeu: 5.594 ± 0.769
1.463LysMet: 1.463 ± 0.375
0.775LysAsn: 0.775 ± 0.226
2.066LysPro: 2.066 ± 0.378
1.291LysGln: 1.291 ± 0.342
2.84LysArg: 2.84 ± 0.498
2.84LysSer: 2.84 ± 0.436
2.754LysThr: 2.754 ± 0.477
4.131LysVal: 4.131 ± 0.589
0.602LysTrp: 0.602 ± 0.196
1.377LysTyr: 1.377 ± 0.33
0.0LysXaa: 0.0 ± 0.0
Leu
11.705LeuAla: 11.705 ± 1.111
0.947LeuCys: 0.947 ± 0.283
5.508LeuAsp: 5.508 ± 0.588
4.303LeuGlu: 4.303 ± 0.477
1.98LeuPhe: 1.98 ± 0.451
6.971LeuGly: 6.971 ± 0.857
1.549LeuHis: 1.549 ± 0.376
4.648LeuIle: 4.648 ± 0.566
3.873LeuLys: 3.873 ± 0.576
7.574LeuLeu: 7.574 ± 0.761
1.98LeuMet: 1.98 ± 0.381
3.443LeuAsn: 3.443 ± 0.542
5.594LeuPro: 5.594 ± 0.718
4.303LeuGln: 4.303 ± 0.622
5.939LeuArg: 5.939 ± 0.551
5.852LeuSer: 5.852 ± 0.645
4.303LeuThr: 4.303 ± 0.461
7.316LeuVal: 7.316 ± 0.78
1.119LeuTrp: 1.119 ± 0.418
1.463LeuTyr: 1.463 ± 0.425
0.0LeuXaa: 0.0 ± 0.0
Met
4.217MetAla: 4.217 ± 0.604
0.344MetCys: 0.344 ± 0.143
2.668MetAsp: 2.668 ± 0.424
0.775MetGlu: 0.775 ± 0.237
0.516MetPhe: 0.516 ± 0.196
1.893MetGly: 1.893 ± 0.365
0.344MetHis: 0.344 ± 0.141
0.775MetIle: 0.775 ± 0.234
1.205MetLys: 1.205 ± 0.277
2.324MetLeu: 2.324 ± 0.498
0.258MetMet: 0.258 ± 0.171
0.775MetAsn: 0.775 ± 0.257
2.066MetPro: 2.066 ± 0.365
1.119MetGln: 1.119 ± 0.361
2.582MetArg: 2.582 ± 0.406
2.152MetSer: 2.152 ± 0.468
1.807MetThr: 1.807 ± 0.506
1.549MetVal: 1.549 ± 0.345
0.516MetTrp: 0.516 ± 0.181
0.344MetTyr: 0.344 ± 0.17
0.0MetXaa: 0.0 ± 0.0
Asn
3.098AsnAla: 3.098 ± 0.523
0.172AsnCys: 0.172 ± 0.099
2.41AsnAsp: 2.41 ± 0.482
1.549AsnGlu: 1.549 ± 0.317
0.861AsnPhe: 0.861 ± 0.27
3.184AsnGly: 3.184 ± 0.515
0.516AsnHis: 0.516 ± 0.191
0.861AsnIle: 0.861 ± 0.297
1.721AsnLys: 1.721 ± 0.303
2.754AsnLeu: 2.754 ± 0.384
0.947AsnMet: 0.947 ± 0.274
1.463AsnAsn: 1.463 ± 0.301
2.324AsnPro: 2.324 ± 0.448
1.549AsnGln: 1.549 ± 0.443
1.549AsnArg: 1.549 ± 0.333
2.152AsnSer: 2.152 ± 0.544
2.066AsnThr: 2.066 ± 0.329
1.893AsnVal: 1.893 ± 0.363
0.689AsnTrp: 0.689 ± 0.231
1.119AsnTyr: 1.119 ± 0.262
0.0AsnXaa: 0.0 ± 0.0
Pro
6.025ProAla: 6.025 ± 0.782
0.258ProCys: 0.258 ± 0.143
2.582ProAsp: 2.582 ± 0.504
3.184ProGlu: 3.184 ± 0.542
1.033ProPhe: 1.033 ± 0.316
3.529ProGly: 3.529 ± 0.505
1.377ProHis: 1.377 ± 0.366
1.98ProIle: 1.98 ± 0.322
2.84ProLys: 2.84 ± 0.507
3.873ProLeu: 3.873 ± 0.474
1.463ProMet: 1.463 ± 0.279
1.98ProAsn: 1.98 ± 0.38
1.205ProPro: 1.205 ± 0.328
1.893ProGln: 1.893 ± 0.317
1.463ProArg: 1.463 ± 0.341
1.807ProSer: 1.807 ± 0.308
2.926ProThr: 2.926 ± 0.518
3.701ProVal: 3.701 ± 0.488
0.775ProTrp: 0.775 ± 0.248
1.119ProTyr: 1.119 ± 0.308
0.0ProXaa: 0.0 ± 0.0
Gln
5.852GlnAla: 5.852 ± 0.631
0.172GlnCys: 0.172 ± 0.117
2.41GlnAsp: 2.41 ± 0.352
2.066GlnGlu: 2.066 ± 0.415
1.807GlnPhe: 1.807 ± 0.335
2.41GlnGly: 2.41 ± 0.426
0.861GlnHis: 0.861 ± 0.241
1.549GlnIle: 1.549 ± 0.366
1.893GlnLys: 1.893 ± 0.365
3.873GlnLeu: 3.873 ± 0.584
1.033GlnMet: 1.033 ± 0.273
1.549GlnAsn: 1.549 ± 0.444
2.066GlnPro: 2.066 ± 0.375
1.807GlnGln: 1.807 ± 0.432
3.098GlnArg: 3.098 ± 0.575
2.754GlnSer: 2.754 ± 0.475
2.324GlnThr: 2.324 ± 0.388
2.926GlnVal: 2.926 ± 0.53
1.033GlnTrp: 1.033 ± 0.306
0.947GlnTyr: 0.947 ± 0.289
0.0GlnXaa: 0.0 ± 0.0
Arg
7.746ArgAla: 7.746 ± 0.824
0.775ArgCys: 0.775 ± 0.232
3.271ArgAsp: 3.271 ± 0.683
3.357ArgGlu: 3.357 ± 0.508
1.807ArgPhe: 1.807 ± 0.319
4.045ArgGly: 4.045 ± 0.522
1.377ArgHis: 1.377 ± 0.351
2.926ArgIle: 2.926 ± 0.523
2.152ArgLys: 2.152 ± 0.356
7.143ArgLeu: 7.143 ± 0.873
2.324ArgMet: 2.324 ± 0.412
2.238ArgAsn: 2.238 ± 0.399
3.012ArgPro: 3.012 ± 0.459
3.529ArgGln: 3.529 ± 0.555
3.787ArgArg: 3.787 ± 0.664
2.324ArgSer: 2.324 ± 0.367
3.184ArgThr: 3.184 ± 0.568
5.336ArgVal: 5.336 ± 0.874
1.205ArgTrp: 1.205 ± 0.333
1.98ArgTyr: 1.98 ± 0.371
0.0ArgXaa: 0.0 ± 0.0
Ser
6.025SerAla: 6.025 ± 0.657
0.516SerCys: 0.516 ± 0.197
3.443SerAsp: 3.443 ± 0.506
2.582SerGlu: 2.582 ± 0.491
2.152SerPhe: 2.152 ± 0.441
4.82SerGly: 4.82 ± 0.649
0.602SerHis: 0.602 ± 0.176
2.496SerIle: 2.496 ± 0.369
3.098SerLys: 3.098 ± 0.487
5.25SerLeu: 5.25 ± 0.711
1.807SerMet: 1.807 ± 0.384
2.324SerAsn: 2.324 ± 0.489
2.668SerPro: 2.668 ± 0.572
2.066SerGln: 2.066 ± 0.454
3.443SerArg: 3.443 ± 0.508
2.754SerSer: 2.754 ± 0.557
4.648SerThr: 4.648 ± 0.521
4.303SerVal: 4.303 ± 0.76
1.119SerTrp: 1.119 ± 0.299
1.291SerTyr: 1.291 ± 0.321
0.0SerXaa: 0.0 ± 0.0
Thr
7.832ThrAla: 7.832 ± 0.798
0.602ThrCys: 0.602 ± 0.203
2.926ThrAsp: 2.926 ± 0.492
3.443ThrGlu: 3.443 ± 0.523
1.807ThrPhe: 1.807 ± 0.433
5.594ThrGly: 5.594 ± 0.699
1.205ThrHis: 1.205 ± 0.242
2.066ThrIle: 2.066 ± 0.383
2.668ThrLys: 2.668 ± 0.518
6.713ThrLeu: 6.713 ± 0.657
1.119ThrMet: 1.119 ± 0.242
2.152ThrAsn: 2.152 ± 0.363
3.443ThrPro: 3.443 ± 0.435
1.721ThrGln: 1.721 ± 0.322
2.754ThrArg: 2.754 ± 0.353
3.357ThrSer: 3.357 ± 0.576
2.754ThrThr: 2.754 ± 0.458
5.68ThrVal: 5.68 ± 0.671
0.344ThrTrp: 0.344 ± 0.152
1.635ThrTyr: 1.635 ± 0.339
0.0ThrXaa: 0.0 ± 0.0
Val
9.209ValAla: 9.209 ± 0.682
0.43ValCys: 0.43 ± 0.177
3.615ValAsp: 3.615 ± 0.513
3.615ValGlu: 3.615 ± 0.655
1.807ValPhe: 1.807 ± 0.388
5.939ValGly: 5.939 ± 0.673
0.947ValHis: 0.947 ± 0.234
3.443ValIle: 3.443 ± 0.522
2.926ValLys: 2.926 ± 0.641
5.939ValLeu: 5.939 ± 0.799
2.066ValMet: 2.066 ± 0.382
3.012ValAsn: 3.012 ± 0.578
2.754ValPro: 2.754 ± 0.47
2.582ValGln: 2.582 ± 0.387
5.422ValArg: 5.422 ± 0.604
5.078ValSer: 5.078 ± 0.659
6.799ValThr: 6.799 ± 0.96
5.25ValVal: 5.25 ± 0.66
1.205ValTrp: 1.205 ± 0.335
1.291ValTyr: 1.291 ± 0.348
0.0ValXaa: 0.0 ± 0.0
Trp
1.463TrpAla: 1.463 ± 0.387
0.172TrpCys: 0.172 ± 0.139
0.344TrpAsp: 0.344 ± 0.189
0.689TrpGlu: 0.689 ± 0.205
0.43TrpPhe: 0.43 ± 0.219
1.463TrpGly: 1.463 ± 0.403
0.344TrpHis: 0.344 ± 0.157
0.947TrpIle: 0.947 ± 0.27
0.258TrpLys: 0.258 ± 0.148
2.926TrpLeu: 2.926 ± 0.458
0.516TrpMet: 0.516 ± 0.181
0.258TrpAsn: 0.258 ± 0.14
0.861TrpPro: 0.861 ± 0.317
0.947TrpGln: 0.947 ± 0.275
1.033TrpArg: 1.033 ± 0.297
0.516TrpSer: 0.516 ± 0.235
0.689TrpThr: 0.689 ± 0.207
1.721TrpVal: 1.721 ± 0.361
0.172TrpTrp: 0.172 ± 0.112
0.086TrpTyr: 0.086 ± 0.065
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.324TyrAla: 2.324 ± 0.357
0.258TyrCys: 0.258 ± 0.12
2.238TyrAsp: 2.238 ± 0.402
1.549TyrGlu: 1.549 ± 0.355
1.119TyrPhe: 1.119 ± 0.276
2.066TyrGly: 2.066 ± 0.323
0.258TyrHis: 0.258 ± 0.148
0.775TyrIle: 0.775 ± 0.233
1.033TyrLys: 1.033 ± 0.279
2.066TyrLeu: 2.066 ± 0.422
0.689TyrMet: 0.689 ± 0.21
0.947TyrAsn: 0.947 ± 0.307
1.291TyrPro: 1.291 ± 0.314
1.033TyrGln: 1.033 ± 0.262
2.238TyrArg: 2.238 ± 0.406
1.721TyrSer: 1.721 ± 0.34
1.033TyrThr: 1.033 ± 0.39
2.066TyrVal: 2.066 ± 0.425
0.172TyrTrp: 0.172 ± 0.122
0.516TyrTyr: 0.516 ± 0.182
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (11620 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski