Allomyces macrogynus (strain ATCC 38327) (Allomyces javanicus var. macrogynus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Blastocladiomycota; Blastocladiomycota incertae sedis; Blastocladiomycetes; Blastocladiales; Blastocladiaceae; Allomyces; Allomyces macrogynus

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19092 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L0SPN8|A0A0L0SPN8_ALLM3 Uncharacterized protein OS=Allomyces macrogynus (strain ATCC 38327) OX=578462 GN=AMAG_19162 PE=4 SV=1
MM1 pKa = 6.93PTAPRR6 pKa = 11.84RR7 pKa = 11.84PRR9 pKa = 11.84PTRR12 pKa = 11.84ILAILATAALFAASFAHH29 pKa = 6.24ARR31 pKa = 11.84PVHH34 pKa = 6.4PYY36 pKa = 8.92GPPVAVPSRR45 pKa = 11.84APTSLDD51 pKa = 2.98AATPPMPVIVPPITSGSSTPDD72 pKa = 2.93VPVPVITRR80 pKa = 11.84PSGSTLSASTSVSSAMPTASEE101 pKa = 4.54SPRR104 pKa = 11.84SSVPSATTMASSAVYY119 pKa = 7.95TVPSASSTLVSSTSWPSTAIGTATTPIARR148 pKa = 11.84STAPSSVSTGGQVTATGTAIATSTAIITGSAPSPSARR185 pKa = 11.84TTTGTASATTSASATTSASATVSAPLSTNLSTASVGTTATTAYY228 pKa = 8.8GTDD231 pKa = 3.66TQSSSASSAIASTTAIAFTPYY252 pKa = 9.55PSSSTLPASEE262 pKa = 4.75SNGSEE267 pKa = 4.04LPTPTTTRR275 pKa = 11.84SADD278 pKa = 3.39VTTWPVDD285 pKa = 3.29PATTSLSTTSALPVPTVAPPASSDD309 pKa = 3.31STRR312 pKa = 11.84PTTGTYY318 pKa = 10.56VYY320 pKa = 10.39GAPTDD325 pKa = 3.93SASATPAPSTGTFVYY340 pKa = 10.56GSDD343 pKa = 3.45PTNAPVASPVSPGYY357 pKa = 10.45DD358 pKa = 2.76LGVGPTPTARR368 pKa = 11.84QDD370 pKa = 3.51PVASIPVATVTTIVVDD386 pKa = 3.92GEE388 pKa = 4.88VITSTVLMTPPADD401 pKa = 3.57ATPAPSPPAGGKK413 pKa = 9.23DD414 pKa = 3.49DD415 pKa = 5.44KK416 pKa = 11.15CDD418 pKa = 3.84CVCDD422 pKa = 4.96DD423 pKa = 5.53DD424 pKa = 5.61EE425 pKa = 4.96EE426 pKa = 4.43PANGDD431 pKa = 3.45DD432 pKa = 4.75CDD434 pKa = 4.07EE435 pKa = 4.59EE436 pKa = 5.13EE437 pKa = 4.36PAEE440 pKa = 4.52DD441 pKa = 4.28CDD443 pKa = 3.95EE444 pKa = 4.46EE445 pKa = 5.29EE446 pKa = 4.28PAEE449 pKa = 4.52DD450 pKa = 4.28CDD452 pKa = 3.95EE453 pKa = 4.46EE454 pKa = 5.29EE455 pKa = 4.52PAEE458 pKa = 4.48DD459 pKa = 4.5CDD461 pKa = 5.4DD462 pKa = 4.28EE463 pKa = 4.75EE464 pKa = 5.75EE465 pKa = 4.14EE466 pKa = 5.56AADD469 pKa = 4.82DD470 pKa = 4.51DD471 pKa = 4.55CDD473 pKa = 4.58EE474 pKa = 5.01EE475 pKa = 4.7GTTAAEE481 pKa = 4.3DD482 pKa = 3.84CTDD485 pKa = 3.39EE486 pKa = 4.31VVTVVVEE493 pKa = 3.94QDD495 pKa = 3.94CDD497 pKa = 3.9DD498 pKa = 5.07ADD500 pKa = 3.78PAAAKK505 pKa = 10.14GALEE509 pKa = 4.23VSTTAADD516 pKa = 3.42ATLTEE521 pKa = 4.61FAPVATTVAAEE532 pKa = 4.18TSSAASVITTSAPAAPASTSAAAPASTSSTAANTAAPAATPVVGTSEE579 pKa = 3.87NFYY582 pKa = 11.51

Molecular weight: 57.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L0SEK3|A0A0L0SEK3_ALLM3 Uncharacterized protein OS=Allomyces macrogynus (strain ATCC 38327) OX=578462 GN=AMAG_06638 PE=4 SV=1
MM1 pKa = 7.75AGNPRR6 pKa = 11.84PTRR9 pKa = 11.84PTRR12 pKa = 11.84SRR14 pKa = 11.84RR15 pKa = 11.84SPNKK19 pKa = 9.02RR20 pKa = 11.84FSKK23 pKa = 9.78ISSRR27 pKa = 11.84ISARR31 pKa = 11.84RR32 pKa = 11.84GSSRR36 pKa = 11.84RR37 pKa = 11.84SCAVPRR43 pKa = 11.84RR44 pKa = 11.84IRR46 pKa = 11.84WRR48 pKa = 11.84RR49 pKa = 11.84SRR51 pKa = 11.84RR52 pKa = 11.84PRR54 pKa = 11.84RR55 pKa = 11.84ARR57 pKa = 11.84AATRR61 pKa = 11.84RR62 pKa = 11.84GRR64 pKa = 11.84RR65 pKa = 11.84AGRR68 pKa = 11.84GRR70 pKa = 11.84RR71 pKa = 11.84TSCTTRR77 pKa = 11.84PRR79 pKa = 11.84NSRR82 pKa = 11.84RR83 pKa = 11.84VPRR86 pKa = 11.84RR87 pKa = 11.84ARR89 pKa = 11.84RR90 pKa = 11.84ARR92 pKa = 11.84IWAVPAGRR100 pKa = 11.84RR101 pKa = 11.84RR102 pKa = 11.84RR103 pKa = 11.84AGRR106 pKa = 11.84GVTT109 pKa = 3.49

Molecular weight: 12.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18611

481

19092

9242631

49

6944

484.1

52.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.312 ± 0.033

1.114 ± 0.006

5.842 ± 0.014

4.618 ± 0.016

2.992 ± 0.012

6.253 ± 0.015

2.743 ± 0.011

3.494 ± 0.013

3.534 ± 0.017

9.081 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.289 ± 0.007

2.58 ± 0.008

7.425 ± 0.027

3.588 ± 0.016

6.456 ± 0.016

7.215 ± 0.02

6.518 ± 0.015

7.565 ± 0.015

1.233 ± 0.006

2.147 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski