Ustilaginoidea virens RNA virus M
Average proteome isoelectric point is 5.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A097I5B9|A0A097I5B9_9VIRU RNA-directed RNA polymerase OS=Ustilaginoidea virens RNA virus M OX=1561172 PE=4 SV=1
MM1 pKa = 7.47 SEE3 pKa = 4.27 YY4 pKa = 10.56 VPPSIEE10 pKa = 4.74 DD11 pKa = 3.34 LWKK14 pKa = 10.12 HH15 pKa = 5.04 LQGFGANPIALRR27 pKa = 11.84 DD28 pKa = 4.18 HH29 pKa = 6.0 ITVPVTEE36 pKa = 4.62 MLSEE40 pKa = 4.5 LRR42 pKa = 11.84 GLDD45 pKa = 3.38 LGRR48 pKa = 11.84 GRR50 pKa = 11.84 IAGWLEE56 pKa = 3.62 PEE58 pKa = 4.25 NILRR62 pKa = 11.84 VEE64 pKa = 4.51 VPAATSPGIRR74 pKa = 11.84 WKK76 pKa = 10.87 KK77 pKa = 10.21 LGYY80 pKa = 7.89 KK81 pKa = 8.13 TKK83 pKa = 10.39 RR84 pKa = 11.84 DD85 pKa = 3.57 AMLQAAVEE93 pKa = 3.96 ARR95 pKa = 11.84 GNIVRR100 pKa = 11.84 MRR102 pKa = 11.84 DD103 pKa = 3.05 VGGGYY108 pKa = 8.53 EE109 pKa = 4.06 VPPCGVAGRR118 pKa = 11.84 GKK120 pKa = 10.03 RR121 pKa = 11.84 VPIDD125 pKa = 3.41 RR126 pKa = 11.84 PSDD129 pKa = 3.62 GEE131 pKa = 4.0 RR132 pKa = 11.84 KK133 pKa = 8.69 EE134 pKa = 3.95 GRR136 pKa = 11.84 FIVMPDD142 pKa = 3.48 LVRR145 pKa = 11.84 HH146 pKa = 5.6 LMGMIAVQPLMKK158 pKa = 10.1 KK159 pKa = 10.09 IKK161 pKa = 10.22 QADD164 pKa = 3.56 KK165 pKa = 11.3 SNGGVMLGMGPFGGSYY181 pKa = 10.83 DD182 pKa = 3.66 RR183 pKa = 11.84 LAEE186 pKa = 4.01 YY187 pKa = 10.84 ASGGKK192 pKa = 10.05 AYY194 pKa = 10.86 LCIDD198 pKa = 4.32 FKK200 pKa = 11.46 KK201 pKa = 10.48 FDD203 pKa = 3.52 QSVPRR208 pKa = 11.84 SVLRR212 pKa = 11.84 AVMQRR217 pKa = 11.84 VRR219 pKa = 11.84 EE220 pKa = 4.05 EE221 pKa = 3.77 FDD223 pKa = 3.38 EE224 pKa = 4.82 EE225 pKa = 4.26 EE226 pKa = 4.81 GSDD229 pKa = 4.05 GYY231 pKa = 10.05 WRR233 pKa = 11.84 SEE235 pKa = 3.8 FKK237 pKa = 10.88 QLVDD241 pKa = 4.03 TEE243 pKa = 4.31 VAMPDD248 pKa = 2.59 GHH250 pKa = 6.59 TYY252 pKa = 10.81 AKK254 pKa = 10.18 RR255 pKa = 11.84 AGVSSGDD262 pKa = 3.16 PWTSIVGSVANWLVLRR278 pKa = 11.84 IALKK282 pKa = 10.64 RR283 pKa = 11.84 LGVDD287 pKa = 3.08 ARR289 pKa = 11.84 IWTFGDD295 pKa = 3.46 DD296 pKa = 3.39 SLIAVDD302 pKa = 3.82 TDD304 pKa = 3.77 VPSEE308 pKa = 4.07 SWLLEE313 pKa = 3.71 AVARR317 pKa = 11.84 EE318 pKa = 4.09 LKK320 pKa = 10.53 SVFGMTVSKK329 pKa = 10.08 EE330 pKa = 3.6 KK331 pKa = 10.84 SIATRR336 pKa = 11.84 HH337 pKa = 4.88 LVGVFEE343 pKa = 4.35 EE344 pKa = 4.87 PEE346 pKa = 4.02 VGQTANFLSAYY357 pKa = 9.41 FLQTGTSVVPVRR369 pKa = 11.84 PIQDD373 pKa = 3.45 LHH375 pKa = 8.27 EE376 pKa = 4.67 LMLVPEE382 pKa = 4.22 RR383 pKa = 11.84 RR384 pKa = 11.84 KK385 pKa = 9.72 DD386 pKa = 3.78 TVWWEE391 pKa = 4.0 VARR394 pKa = 11.84 TAMAYY399 pKa = 10.38 LVFFWNDD406 pKa = 3.02 SARR409 pKa = 11.84 MTLEE413 pKa = 3.89 WYY415 pKa = 9.27 WDD417 pKa = 3.77 FLHH420 pKa = 7.19 RR421 pKa = 11.84 EE422 pKa = 3.94 YY423 pKa = 10.64 EE424 pKa = 4.24 IPQLRR429 pKa = 11.84 GDD431 pKa = 3.94 PEE433 pKa = 3.95 ILGMLRR439 pKa = 11.84 LMDD442 pKa = 4.95 LPWDD446 pKa = 3.64 QFKK449 pKa = 11.25 EE450 pKa = 3.89 EE451 pKa = 3.91 WLIRR455 pKa = 11.84 LPYY458 pKa = 9.68 RR459 pKa = 11.84 YY460 pKa = 9.16 EE461 pKa = 3.85 VEE463 pKa = 4.03 LLYY466 pKa = 11.18 SHH468 pKa = 7.32 GYY470 pKa = 5.68 TTFFPPALWALTYY483 pKa = 10.21 KK484 pKa = 10.84 DD485 pKa = 4.76 HH486 pKa = 7.28 VPLDD490 pKa = 4.11 GGNKK494 pKa = 8.64 VWWEE498 pKa = 4.0 LEE500 pKa = 4.14 GQQ502 pKa = 3.54
Molecular weight: 57.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.578
IPC2_protein 5.563
IPC_protein 5.563
Toseland 5.817
ProMoST 5.804
Dawson 5.69
Bjellqvist 5.753
Wikipedia 5.639
Rodwell 5.652
Grimsley 5.931
Solomon 5.703
Lehninger 5.677
Nozaki 5.893
DTASelect 6.071
Thurlkill 5.957
EMBOSS 5.919
Sillero 5.995
Patrickios 4.418
IPC_peptide 5.715
IPC2_peptide 5.982
IPC2.peptide.svr19 5.997
Protein with the highest isoelectric point:
>tr|A0A097I5B9|A0A097I5B9_9VIRU RNA-directed RNA polymerase OS=Ustilaginoidea virens RNA virus M OX=1561172 PE=4 SV=1
MM1 pKa = 7.47 SEE3 pKa = 4.27 YY4 pKa = 10.56 VPPSIEE10 pKa = 4.74 DD11 pKa = 3.34 LWKK14 pKa = 10.12 HH15 pKa = 5.04 LQGFGANPIALRR27 pKa = 11.84 DD28 pKa = 4.18 HH29 pKa = 6.0 ITVPVTEE36 pKa = 4.62 MLSEE40 pKa = 4.5 LRR42 pKa = 11.84 GLDD45 pKa = 3.38 LGRR48 pKa = 11.84 GRR50 pKa = 11.84 IAGWLEE56 pKa = 3.62 PEE58 pKa = 4.25 NILRR62 pKa = 11.84 VEE64 pKa = 4.51 VPAATSPGIRR74 pKa = 11.84 WKK76 pKa = 10.87 KK77 pKa = 10.21 LGYY80 pKa = 7.89 KK81 pKa = 8.13 TKK83 pKa = 10.39 RR84 pKa = 11.84 DD85 pKa = 3.57 AMLQAAVEE93 pKa = 3.96 ARR95 pKa = 11.84 GNIVRR100 pKa = 11.84 MRR102 pKa = 11.84 DD103 pKa = 3.05 VGGGYY108 pKa = 8.53 EE109 pKa = 4.06 VPPCGVAGRR118 pKa = 11.84 GKK120 pKa = 10.03 RR121 pKa = 11.84 VPIDD125 pKa = 3.41 RR126 pKa = 11.84 PSDD129 pKa = 3.62 GEE131 pKa = 4.0 RR132 pKa = 11.84 KK133 pKa = 8.69 EE134 pKa = 3.95 GRR136 pKa = 11.84 FIVMPDD142 pKa = 3.48 LVRR145 pKa = 11.84 HH146 pKa = 5.6 LMGMIAVQPLMKK158 pKa = 10.1 KK159 pKa = 10.09 IKK161 pKa = 10.22 QADD164 pKa = 3.56 KK165 pKa = 11.3 SNGGVMLGMGPFGGSYY181 pKa = 10.83 DD182 pKa = 3.66 RR183 pKa = 11.84 LAEE186 pKa = 4.01 YY187 pKa = 10.84 ASGGKK192 pKa = 10.05 AYY194 pKa = 10.86 LCIDD198 pKa = 4.32 FKK200 pKa = 11.46 KK201 pKa = 10.48 FDD203 pKa = 3.52 QSVPRR208 pKa = 11.84 SVLRR212 pKa = 11.84 AVMQRR217 pKa = 11.84 VRR219 pKa = 11.84 EE220 pKa = 4.05 EE221 pKa = 3.77 FDD223 pKa = 3.38 EE224 pKa = 4.82 EE225 pKa = 4.26 EE226 pKa = 4.81 GSDD229 pKa = 4.05 GYY231 pKa = 10.05 WRR233 pKa = 11.84 SEE235 pKa = 3.8 FKK237 pKa = 10.88 QLVDD241 pKa = 4.03 TEE243 pKa = 4.31 VAMPDD248 pKa = 2.59 GHH250 pKa = 6.59 TYY252 pKa = 10.81 AKK254 pKa = 10.18 RR255 pKa = 11.84 AGVSSGDD262 pKa = 3.16 PWTSIVGSVANWLVLRR278 pKa = 11.84 IALKK282 pKa = 10.64 RR283 pKa = 11.84 LGVDD287 pKa = 3.08 ARR289 pKa = 11.84 IWTFGDD295 pKa = 3.46 DD296 pKa = 3.39 SLIAVDD302 pKa = 3.82 TDD304 pKa = 3.77 VPSEE308 pKa = 4.07 SWLLEE313 pKa = 3.71 AVARR317 pKa = 11.84 EE318 pKa = 4.09 LKK320 pKa = 10.53 SVFGMTVSKK329 pKa = 10.08 EE330 pKa = 3.6 KK331 pKa = 10.84 SIATRR336 pKa = 11.84 HH337 pKa = 4.88 LVGVFEE343 pKa = 4.35 EE344 pKa = 4.87 PEE346 pKa = 4.02 VGQTANFLSAYY357 pKa = 9.41 FLQTGTSVVPVRR369 pKa = 11.84 PIQDD373 pKa = 3.45 LHH375 pKa = 8.27 EE376 pKa = 4.67 LMLVPEE382 pKa = 4.22 RR383 pKa = 11.84 RR384 pKa = 11.84 KK385 pKa = 9.72 DD386 pKa = 3.78 TVWWEE391 pKa = 4.0 VARR394 pKa = 11.84 TAMAYY399 pKa = 10.38 LVFFWNDD406 pKa = 3.02 SARR409 pKa = 11.84 MTLEE413 pKa = 3.89 WYY415 pKa = 9.27 WDD417 pKa = 3.77 FLHH420 pKa = 7.19 RR421 pKa = 11.84 EE422 pKa = 3.94 YY423 pKa = 10.64 EE424 pKa = 4.24 IPQLRR429 pKa = 11.84 GDD431 pKa = 3.94 PEE433 pKa = 3.95 ILGMLRR439 pKa = 11.84 LMDD442 pKa = 4.95 LPWDD446 pKa = 3.64 QFKK449 pKa = 11.25 EE450 pKa = 3.89 EE451 pKa = 3.91 WLIRR455 pKa = 11.84 LPYY458 pKa = 9.68 RR459 pKa = 11.84 YY460 pKa = 9.16 EE461 pKa = 3.85 VEE463 pKa = 4.03 LLYY466 pKa = 11.18 SHH468 pKa = 7.32 GYY470 pKa = 5.68 TTFFPPALWALTYY483 pKa = 10.21 KK484 pKa = 10.84 DD485 pKa = 4.76 HH486 pKa = 7.28 VPLDD490 pKa = 4.11 GGNKK494 pKa = 8.64 VWWEE498 pKa = 4.0 LEE500 pKa = 4.14 GQQ502 pKa = 3.54
Molecular weight: 57.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.578
IPC2_protein 5.563
IPC_protein 5.563
Toseland 5.817
ProMoST 5.804
Dawson 5.69
Bjellqvist 5.753
Wikipedia 5.639
Rodwell 5.652
Grimsley 5.931
Solomon 5.703
Lehninger 5.677
Nozaki 5.893
DTASelect 6.071
Thurlkill 5.957
EMBOSS 5.919
Sillero 5.995
Patrickios 4.418
IPC_peptide 5.715
IPC2_peptide 5.982
IPC2.peptide.svr19 5.997
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
502
502
502
502.0
57.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.773 ± 0.0
0.398 ± 0.0
6.175 ± 0.0
7.769 ± 0.0
3.586 ± 0.0
8.765 ± 0.0
1.793 ± 0.0
4.183 ± 0.0
4.781 ± 0.0
9.761 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.586 ± 0.0
1.594 ± 0.0
5.777 ± 0.0
2.59 ± 0.0
7.57 ± 0.0
5.179 ± 0.0
3.984 ± 0.0
8.765 ± 0.0
3.586 ± 0.0
3.386 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here