Candidatus Marinamargulisbacteria bacterium SCGC AG-333-B06

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Candidatus Margulisbacteria; Candidatus Marinamargulisbacteria

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 544 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328R783|A0A328R783_9BACT NTP_transferase domain-containing protein OS=Candidatus Marinamargulisbacteria bacterium SCGC AG-333-B06 OX=2184342 GN=DID74_01640 PE=4 SV=1
MM1 pKa = 7.83PKK3 pKa = 9.89HH4 pKa = 6.53LFRR7 pKa = 11.84ILCACFLSLSLLLTSCGNLLDD28 pKa = 5.05DD29 pKa = 5.34ASSSDD34 pKa = 3.37SDD36 pKa = 3.69TSRR39 pKa = 11.84GALSITDD46 pKa = 3.57ITMDD50 pKa = 3.89SYY52 pKa = 11.17PVSFMEE58 pKa = 5.29EE59 pKa = 3.38IQKK62 pKa = 10.79NIEE65 pKa = 3.88ASAGEE70 pKa = 4.64LFTAASYY77 pKa = 11.04SVNQYY82 pKa = 10.18NLVYY86 pKa = 9.27HH87 pKa = 6.92TIDD90 pKa = 3.02AKK92 pKa = 11.42GNATLASAKK101 pKa = 10.56LLIPDD106 pKa = 4.78ANTDD110 pKa = 3.49LDD112 pKa = 4.28LVVYY116 pKa = 9.09LHH118 pKa = 6.52GTTSLDD124 pKa = 3.12ADD126 pKa = 4.09APTNAYY132 pKa = 10.65ADD134 pKa = 3.84FSDD137 pKa = 4.98FNGDD141 pKa = 3.42DD142 pKa = 3.48ATTIDD147 pKa = 5.01HH148 pKa = 7.05NIADD152 pKa = 4.07MKK154 pKa = 8.29EE155 pKa = 3.99TAMLMSFVSDD165 pKa = 3.27TNIAVLMPDD174 pKa = 3.37YY175 pKa = 10.97LGFGASEE182 pKa = 4.09GLHH185 pKa = 6.56PYY187 pKa = 8.4MHH189 pKa = 6.93GASLASATIDD199 pKa = 4.14AIRR202 pKa = 11.84AALNFASLSSNPFSLTGDD220 pKa = 3.82FTLTGYY226 pKa = 10.36SEE228 pKa = 4.05GGYY231 pKa = 11.02AVMATHH237 pKa = 7.54KK238 pKa = 10.54EE239 pKa = 3.86LLADD243 pKa = 3.74NGTVEE248 pKa = 4.57SAYY251 pKa = 10.8LDD253 pKa = 3.3IMGSEE258 pKa = 4.57GARR261 pKa = 11.84LKK263 pKa = 10.95AVIPGAPPCNISQRR277 pKa = 11.84MAAVMLPPAGIAYY290 pKa = 7.78PAPAFAAYY298 pKa = 9.55VALAYY303 pKa = 10.84NEE305 pKa = 4.41IYY307 pKa = 10.46DD308 pKa = 3.69IEE310 pKa = 5.04ANISTIFSPTYY321 pKa = 10.57NYY323 pKa = 10.17IIDD326 pKa = 4.88DD327 pKa = 3.85FDD329 pKa = 4.2GSKK332 pKa = 10.79SLTTMTGEE340 pKa = 4.27FLAGGIFPPTPISMFSTEE358 pKa = 4.09FQDD361 pKa = 6.05AILADD366 pKa = 3.65YY367 pKa = 9.44TKK369 pKa = 10.46ILEE372 pKa = 5.05DD373 pKa = 3.61PTATPDD379 pKa = 3.38DD380 pKa = 4.22DD381 pKa = 5.47FYY383 pKa = 11.96QKK385 pKa = 10.69FKK387 pKa = 11.18EE388 pKa = 3.92NDD390 pKa = 3.2VFDD393 pKa = 4.18YY394 pKa = 11.17HH395 pKa = 7.13PGTAYY400 pKa = 11.07YY401 pKa = 9.76EE402 pKa = 4.39IIYY405 pKa = 8.04TASDD409 pKa = 3.9EE410 pKa = 4.27IVPSINATEE419 pKa = 3.96AAEE422 pKa = 4.01ALAVPGRR429 pKa = 11.84SVNILFDD436 pKa = 3.92PAEE439 pKa = 4.31ISSDD443 pKa = 3.25PTGINLFADD452 pKa = 4.54PQDD455 pKa = 3.63STYY458 pKa = 9.89VTIKK462 pKa = 10.87AGDD465 pKa = 3.79PSLLIHH471 pKa = 6.77SSAAGFYY478 pKa = 9.92IGRR481 pKa = 11.84SFNVSKK487 pKa = 10.77MMLSQQ492 pKa = 3.5

Molecular weight: 52.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328QZQ4|A0A328QZQ4_9BACT AB hydrolase-1 domain-containing protein OS=Candidatus Marinamargulisbacteria bacterium SCGC AG-333-B06 OX=2184342 GN=DID74_01160 PE=4 SV=1
MM1 pKa = 6.54YY2 pKa = 8.73TYY4 pKa = 10.97FSMQSSIISKK14 pKa = 10.77ANLAKK19 pKa = 10.31EE20 pKa = 4.44LAVHH24 pKa = 6.45MGWTQKK30 pKa = 10.88KK31 pKa = 9.5SLLFIDD37 pKa = 5.88DD38 pKa = 3.73CFDD41 pKa = 4.04WIKK44 pKa = 10.93SQMLRR49 pKa = 11.84GHH51 pKa = 7.17RR52 pKa = 11.84IVLSGFGVFQLNLRR66 pKa = 11.84KK67 pKa = 9.5KK68 pKa = 10.26RR69 pKa = 11.84RR70 pKa = 11.84LLHH73 pKa = 6.79PMTQKK78 pKa = 10.69PLLVPEE84 pKa = 4.35RR85 pKa = 11.84LRR87 pKa = 11.84PNFTPSDD94 pKa = 3.81AVLHH98 pKa = 5.9LLNHH102 pKa = 6.87RR103 pKa = 11.84ARR105 pKa = 11.84LL106 pKa = 3.66

Molecular weight: 12.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

544

0

544

177059

37

1667

325.5

36.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.551 ± 0.109

1.296 ± 0.044

5.601 ± 0.096

5.317 ± 0.111

5.085 ± 0.106

5.495 ± 0.105

2.504 ± 0.061

9.116 ± 0.122

7.673 ± 0.135

10.176 ± 0.123

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.176 ± 0.051

5.618 ± 0.098

3.578 ± 0.062

3.852 ± 0.064

3.324 ± 0.081

7.587 ± 0.099

5.603 ± 0.077

5.554 ± 0.084

0.842 ± 0.033

4.053 ± 0.072
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski