Undibacterium piscinae
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3332 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M4A9B6|A0A6M4A9B6_9BURK Argininosuccinate synthase OS=Undibacterium piscinae OX=2495591 GN=argG PE=3 SV=1
MM1 pKa = 7.58 CLICGWVYY9 pKa = 10.83 EE10 pKa = 4.55 EE11 pKa = 5.07 EE12 pKa = 5.12 LGLPDD17 pKa = 5.29 DD18 pKa = 5.83 GIPAGTKK25 pKa = 9.43 WEE27 pKa = 4.21 DD28 pKa = 3.31 VPMNWTCPEE37 pKa = 3.86 CGARR41 pKa = 11.84 KK42 pKa = 9.6 EE43 pKa = 4.09 DD44 pKa = 4.18 FEE46 pKa = 4.4 MLAII50 pKa = 4.58
Molecular weight: 5.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.808
IPC2_protein 3.948
IPC_protein 3.783
Toseland 3.617
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.668
Rodwell 3.63
Grimsley 3.541
Solomon 3.719
Lehninger 3.668
Nozaki 3.897
DTASelect 3.999
Thurlkill 3.681
EMBOSS 3.681
Sillero 3.897
Patrickios 0.604
IPC_peptide 3.719
IPC2_peptide 3.872
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|A0A6M4A7H5|A0A6M4A7H5_9BURK Nucleotide sugar dehydrogenase OS=Undibacterium piscinae OX=2495591 GN=EJG51_013365 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.97 QPSVVRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.57 RR14 pKa = 11.84 THH16 pKa = 5.79 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.77 GRR39 pKa = 11.84 KK40 pKa = 8.8 RR41 pKa = 11.84 LAAA44 pKa = 4.42
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3332
0
3332
1065376
35
3890
319.7
35.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.135 ± 0.054
0.935 ± 0.014
5.113 ± 0.03
5.336 ± 0.041
3.837 ± 0.035
7.308 ± 0.046
2.181 ± 0.022
5.75 ± 0.031
4.67 ± 0.035
10.85 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.694 ± 0.023
3.631 ± 0.039
4.319 ± 0.028
4.454 ± 0.039
5.342 ± 0.037
6.591 ± 0.04
5.146 ± 0.042
6.831 ± 0.034
1.279 ± 0.018
2.599 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here