Catellatospora citrea
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8194 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A419XE78|A0A419XE78_9ACTN L-ascorbate metabolism protein UlaG (Beta-lactamase superfamily) OS=Catellatospora citrea OX=53366 GN=C8E86_0813 PE=4 SV=1
MM1 pKa = 6.6 QTPSLLRR8 pKa = 11.84 RR9 pKa = 11.84 IGVFGALTGVALAAGLAAGSPAQAATTLKK38 pKa = 10.46 VATVICNEE46 pKa = 4.01 EE47 pKa = 3.79 TDD49 pKa = 4.37 EE50 pKa = 5.49 IGDD53 pKa = 3.79 DD54 pKa = 3.53 SPYY57 pKa = 10.78 FLVFAASTTNPGATAFGKK75 pKa = 9.3 WGPGGWDD82 pKa = 3.7 DD83 pKa = 5.49 EE84 pKa = 4.53 IASGDD89 pKa = 3.95 SYY91 pKa = 11.83 SPNASIASGVSSSSWRR107 pKa = 11.84 LISLMVEE114 pKa = 3.69 EE115 pKa = 6.08 DD116 pKa = 3.47 YY117 pKa = 11.79 DD118 pKa = 4.03 NDD120 pKa = 3.48 LTAGEE125 pKa = 4.43 IAWIGNNMYY134 pKa = 10.4 NQYY137 pKa = 10.26 QVSFWKK143 pKa = 8.77 PTSTMISEE151 pKa = 4.1 MRR153 pKa = 11.84 ATLRR157 pKa = 11.84 NTTALYY163 pKa = 10.61 LSNDD167 pKa = 3.66 DD168 pKa = 3.87 MVATASSTIGSYY180 pKa = 10.98 ASYY183 pKa = 10.48 IGDD186 pKa = 3.49 GGNYY190 pKa = 6.84 QVRR193 pKa = 11.84 YY194 pKa = 7.1 TLPP197 pKa = 3.58
Molecular weight: 20.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.806
IPC2_protein 4.05
IPC_protein 3.999
Toseland 3.783
ProMoST 4.151
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.91
Rodwell 3.821
Grimsley 3.706
Solomon 3.973
Lehninger 3.923
Nozaki 4.101
DTASelect 4.317
Thurlkill 3.846
EMBOSS 3.923
Sillero 4.113
Patrickios 0.998
IPC_peptide 3.973
IPC2_peptide 4.088
IPC2.peptide.svr19 3.999
Protein with the highest isoelectric point:
>tr|A0A419XLK6|A0A419XLK6_9ACTN Uncharacterized protein OS=Catellatospora citrea OX=53366 GN=C8E86_3471 PE=4 SV=1
MM1 pKa = 7.28 GSVVKK6 pKa = 10.49 KK7 pKa = 9.45 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.48 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.7 LLRR22 pKa = 11.84 KK23 pKa = 7.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 LGKK33 pKa = 10.04
Molecular weight: 4.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8194
0
8194
2768762
29
5418
337.9
36.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.161 ± 0.042
0.773 ± 0.008
5.987 ± 0.022
4.774 ± 0.028
2.786 ± 0.015
9.223 ± 0.028
2.239 ± 0.014
3.356 ± 0.018
1.834 ± 0.022
10.539 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.746 ± 0.011
1.976 ± 0.024
6.081 ± 0.026
2.997 ± 0.014
7.666 ± 0.031
4.947 ± 0.025
6.314 ± 0.031
8.754 ± 0.025
1.678 ± 0.011
2.17 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here