Klebsiella phage vB_KpnP_IME308

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Drulisvirus; unclassified Drulisvirus

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6CUA7|A0A5J6CUA7_9CAUD Putative scaffolding protein OS=Klebsiella phage vB_KpnP_IME308 OX=2601648 PE=4 SV=1
MM1 pKa = 7.4AFSWQEE7 pKa = 3.86QIKK10 pKa = 10.63SAGTQDD16 pKa = 3.28IQCDD20 pKa = 3.23IEE22 pKa = 4.59YY23 pKa = 10.49LDD25 pKa = 3.51KK26 pKa = 11.27SYY28 pKa = 10.95IHH30 pKa = 6.76VYY32 pKa = 10.56LDD34 pKa = 3.57GVEE37 pKa = 4.31TTGYY41 pKa = 8.08TWTSSTNIRR50 pKa = 11.84LNTALTADD58 pKa = 3.76TTVLLIRR65 pKa = 11.84KK66 pKa = 6.77TEE68 pKa = 3.78RR69 pKa = 11.84EE70 pKa = 3.71YY71 pKa = 11.31LYY73 pKa = 10.39IEE75 pKa = 4.49FASGSPFIEE84 pKa = 4.25VNVDD88 pKa = 3.39SQNTQFLHH96 pKa = 6.59LAQEE100 pKa = 4.35LVEE103 pKa = 4.47GRR105 pKa = 11.84AIPGFYY111 pKa = 10.91GNISMNGYY119 pKa = 10.14RR120 pKa = 11.84ITNVADD126 pKa = 3.81PEE128 pKa = 4.19AQQDD132 pKa = 4.07VATKK136 pKa = 10.5NYY138 pKa = 10.22VDD140 pKa = 4.01TGDD143 pKa = 3.61AALGARR149 pKa = 11.84IDD151 pKa = 3.86AEE153 pKa = 4.15TAARR157 pKa = 11.84KK158 pKa = 9.11AADD161 pKa = 3.18AALDD165 pKa = 3.57VRR167 pKa = 11.84TTNLEE172 pKa = 3.7QTFISGEE179 pKa = 4.13PTVSYY184 pKa = 9.54PWYY187 pKa = 9.36TILTEE192 pKa = 4.29STDD195 pKa = 3.4EE196 pKa = 4.12VTPGLSFTKK205 pKa = 10.41AIVYY209 pKa = 8.14VQGVNQIPGYY219 pKa = 9.53SFEE222 pKa = 4.79VVDD225 pKa = 3.77NTLLFAEE232 pKa = 4.55VLPAGTLVSARR243 pKa = 11.84LGYY246 pKa = 10.12DD247 pKa = 3.06AEE249 pKa = 4.43LSGTYY254 pKa = 8.56ATATALGEE262 pKa = 4.15EE263 pKa = 4.33ASARR267 pKa = 11.84ANADD271 pKa = 3.02AQIILDD277 pKa = 3.7YY278 pKa = 10.51QAAVGAKK285 pKa = 8.89AAKK288 pKa = 9.4GANSDD293 pKa = 3.4ITSLSGLTTPLSKK306 pKa = 10.94AQGGTGNTTGAADD319 pKa = 3.74SAAKK323 pKa = 9.93LAAARR328 pKa = 11.84ALVVDD333 pKa = 4.82LTSTTPANFDD343 pKa = 3.56GTSDD347 pKa = 3.41ATLGATGSLPITKK360 pKa = 9.79GGTGAGDD367 pKa = 3.43AATARR372 pKa = 11.84ANLGAAASGSNGDD385 pKa = 3.38IAALTGLSGGISGRR399 pKa = 11.84TDD401 pKa = 2.99GAAAAAGVVGEE412 pKa = 4.37VLSAATAAAVSVTSGTPLNVLSLSLPAGEE441 pKa = 4.88YY442 pKa = 9.8EE443 pKa = 4.67LEE445 pKa = 4.24SALLVTNSGNVTALSFGVSSTSAVLPSNWYY475 pKa = 9.49DD476 pKa = 3.42LYY478 pKa = 11.51SITTTLAAGNSSRR491 pKa = 11.84QGMSRR496 pKa = 11.84RR497 pKa = 11.84LRR499 pKa = 11.84LSATTTVYY507 pKa = 10.77LVAQASFTGTCTAQGYY523 pKa = 8.16IRR525 pKa = 11.84AMRR528 pKa = 11.84VRR530 pKa = 3.94

Molecular weight: 54.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6CUD7|A0A5J6CUD7_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnP_IME308 OX=2601648 PE=4 SV=1
MM1 pKa = 7.13YY2 pKa = 10.04GKK4 pKa = 9.92NPEE7 pKa = 4.08TLLMRR12 pKa = 11.84KK13 pKa = 8.26QRR15 pKa = 11.84PAIDD19 pKa = 3.54GLARR23 pKa = 11.84EE24 pKa = 4.54YY25 pKa = 10.64SAKK28 pKa = 10.43AALRR32 pKa = 11.84QHH34 pKa = 6.17YY35 pKa = 7.59EE36 pKa = 3.71KK37 pKa = 10.5QAQRR41 pKa = 11.84LGMTLRR47 pKa = 11.84GYY49 pKa = 9.88CFRR52 pKa = 11.84FNVRR56 pKa = 11.84GVVV59 pKa = 3.11

Molecular weight: 6.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

13312

30

1232

271.7

29.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.547 ± 0.552

1.157 ± 0.189

6.085 ± 0.219

5.709 ± 0.395

3.087 ± 0.159

7.745 ± 0.37

1.886 ± 0.215

4.327 ± 0.172

4.928 ± 0.335

8.744 ± 0.254

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.825 ± 0.221

4.034 ± 0.264

3.771 ± 0.241

4.635 ± 0.349

5.934 ± 0.303

6.483 ± 0.372

5.874 ± 0.362

7.039 ± 0.263

1.427 ± 0.119

3.764 ± 0.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski