Staphylococcus phage tp310-3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Peeveelvirus; unclassified Peeveelvirus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7TWM0|A7TWM0_9CAUD Uncharacterized protein OS=Staphylococcus phage tp310-3 OX=445517 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.25NYY4 pKa = 10.4YY5 pKa = 9.86HH6 pKa = 7.24LLSFDD11 pKa = 5.04DD12 pKa = 5.61DD13 pKa = 4.5LANDD17 pKa = 4.17AANDD21 pKa = 4.01LLKK24 pKa = 10.8EE25 pKa = 4.08GWDD28 pKa = 3.34IVHH31 pKa = 6.66VGTKK35 pKa = 9.51LVKK38 pKa = 10.36ILDD41 pKa = 3.92NGQAYY46 pKa = 10.82YY47 pKa = 8.91NTEE50 pKa = 3.97YY51 pKa = 11.47VLGGTKK57 pKa = 9.45NQYY60 pKa = 9.36EE61 pKa = 4.83KK62 pKa = 11.2YY63 pKa = 10.75LEE65 pKa = 4.8DD66 pKa = 4.04CQQSEE71 pKa = 4.31LDD73 pKa = 3.76YY74 pKa = 11.3FF75 pKa = 4.23

Molecular weight: 8.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7TWQ7|A7TWQ7_9CAUD Uncharacterized protein OS=Staphylococcus phage tp310-3 OX=445517 PE=4 SV=1
MM1 pKa = 7.42GARR4 pKa = 11.84IEE6 pKa = 4.59SNNIEE11 pKa = 4.18QGLKK15 pKa = 10.42NAVLKK20 pKa = 10.7MNLNSNVIVKK30 pKa = 10.08AGAMSLVPLLKK41 pKa = 10.86SNTPFANTKK50 pKa = 9.27KK51 pKa = 9.95HH52 pKa = 5.78ARR54 pKa = 11.84DD55 pKa = 3.92HH56 pKa = 6.19IAVSNVKK63 pKa = 8.84TDD65 pKa = 2.81RR66 pKa = 11.84HH67 pKa = 4.91TSEE70 pKa = 4.85KK71 pKa = 10.29IVTIGYY77 pKa = 10.15AKK79 pKa = 10.15GVSHH83 pKa = 7.61RR84 pKa = 11.84IHH86 pKa = 5.72ATEE89 pKa = 4.86FGTMYY94 pKa = 10.3QKK96 pKa = 10.66PQLFITKK103 pKa = 7.84TEE105 pKa = 3.97KK106 pKa = 9.85QGKK109 pKa = 8.76NKK111 pKa = 9.58VLKK114 pKa = 10.18TMLDD118 pKa = 3.43TAKK121 pKa = 10.54RR122 pKa = 11.84LQKK125 pKa = 10.81

Molecular weight: 13.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11107

49

1550

191.5

21.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.465 ± 0.476

0.675 ± 0.134

6.014 ± 0.345

7.833 ± 0.573

3.817 ± 0.228

5.798 ± 0.565

1.729 ± 0.178

7.608 ± 0.252

9.67 ± 0.357

8.04 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.674 ± 0.198

6.536 ± 0.215

2.575 ± 0.189

3.763 ± 0.265

4.457 ± 0.215

5.924 ± 0.294

5.987 ± 0.265

5.771 ± 0.203

1.143 ± 0.158

4.52 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski