Human fecal virus Jorvi3
Average proteome isoelectric point is 7.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220IGD8|A0A220IGD8_9CIRC ATP-dependent helicase Rep OS=Human fecal virus Jorvi3 OX=2017082 PE=3 SV=1
MM1 pKa = 6.71 STAYY5 pKa = 10.44 CFTLNNPEE13 pKa = 4.02 TYY15 pKa = 10.73 LPDD18 pKa = 3.85 FRR20 pKa = 11.84 THH22 pKa = 7.6 DD23 pKa = 3.68 LSQYY27 pKa = 9.55 TNIRR31 pKa = 11.84 YY32 pKa = 9.36 CIYY35 pKa = 10.12 QLEE38 pKa = 4.62 EE39 pKa = 3.91 GTTPHH44 pKa = 5.98 LQGYY48 pKa = 8.16 LQLSRR53 pKa = 11.84 IQKK56 pKa = 9.72 IINIQKK62 pKa = 9.39 QEE64 pKa = 3.99 FFKK67 pKa = 10.9 HH68 pKa = 5.55 CSFRR72 pKa = 11.84 QANGTPEE79 pKa = 3.88 QNIVYY84 pKa = 9.85 CSKK87 pKa = 10.76 KK88 pKa = 10.59 DD89 pKa = 3.21 EE90 pKa = 4.54 TYY92 pKa = 10.82 RR93 pKa = 11.84 EE94 pKa = 4.41 GPWIYY99 pKa = 9.85 GTIKK103 pKa = 10.37 QQGKK107 pKa = 10.38 RR108 pKa = 11.84 NDD110 pKa = 4.1 LEE112 pKa = 4.73 NMAEE116 pKa = 4.54 DD117 pKa = 3.16 ILVNKK122 pKa = 8.22 KK123 pKa = 8.1 TRR125 pKa = 11.84 LEE127 pKa = 3.85 IAKK130 pKa = 9.08 EE131 pKa = 3.89 YY132 pKa = 9.07 PANFMRR138 pKa = 11.84 YY139 pKa = 8.45 PRR141 pKa = 11.84 GINALFDD148 pKa = 3.76 LAIPKK153 pKa = 9.78 RR154 pKa = 11.84 DD155 pKa = 3.5 FKK157 pKa = 11.29 TEE159 pKa = 3.62 VIYY162 pKa = 10.26 IYY164 pKa = 10.86 GSTGTGKK171 pKa = 10.08 SKK173 pKa = 10.47 YY174 pKa = 10.11 AHH176 pKa = 6.11 QFDD179 pKa = 4.09 KK180 pKa = 11.28 NAYY183 pKa = 6.65 TKK185 pKa = 10.88 SFDD188 pKa = 3.85 KK189 pKa = 10.4 WWDD192 pKa = 3.36 QYY194 pKa = 11.77 NGQDD198 pKa = 3.47 TVIMDD203 pKa = 4.78 DD204 pKa = 3.57 YY205 pKa = 11.3 HH206 pKa = 9.09 GEE208 pKa = 3.9 LSFNQLLALTDD219 pKa = 3.73 RR220 pKa = 11.84 YY221 pKa = 10.4 EE222 pKa = 4.29 CRR224 pKa = 11.84 IEE226 pKa = 3.97 YY227 pKa = 10.02 KK228 pKa = 10.74 GSSLQFNSKK237 pKa = 10.07 YY238 pKa = 10.82 LFIISTRR245 pKa = 11.84 LPDD248 pKa = 3.21 DD249 pKa = 4.49 WYY251 pKa = 10.63 PEE253 pKa = 3.85 ISPLRR258 pKa = 11.84 MEE260 pKa = 4.2 EE261 pKa = 4.17 FYY263 pKa = 11.23 RR264 pKa = 11.84 RR265 pKa = 11.84 IDD267 pKa = 3.29 TVIYY271 pKa = 8.5 FTEE274 pKa = 4.21 KK275 pKa = 9.35 EE276 pKa = 4.19 QYY278 pKa = 10.17 QEE280 pKa = 4.02 FHH282 pKa = 6.86 SFSCFKK288 pKa = 10.83 KK289 pKa = 10.51 III291 pKa = 3.87
Molecular weight: 34.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.357
IPC2_protein 6.478
IPC_protein 6.62
Toseland 6.62
ProMoST 7.044
Dawson 7.117
Bjellqvist 7.0
Wikipedia 7.088
Rodwell 7.102
Grimsley 6.693
Solomon 7.146
Lehninger 7.161
Nozaki 7.307
DTASelect 7.38
Thurlkill 7.41
EMBOSS 7.454
Sillero 7.512
Patrickios 3.681
IPC_peptide 7.161
IPC2_peptide 6.942
IPC2.peptide.svr19 6.934
Protein with the highest isoelectric point:
>tr|A0A220IGD8|A0A220IGD8_9CIRC ATP-dependent helicase Rep OS=Human fecal virus Jorvi3 OX=2017082 PE=3 SV=1
MM1 pKa = 7.79 PGFKK5 pKa = 9.67 RR6 pKa = 11.84 SRR8 pKa = 11.84 RR9 pKa = 11.84 LYY11 pKa = 9.13 SSKK14 pKa = 10.03 RR15 pKa = 11.84 RR16 pKa = 11.84 YY17 pKa = 8.99 GRR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 TFKK24 pKa = 10.84 KK25 pKa = 9.49 VMHH28 pKa = 5.08 YY29 pKa = 10.01 RR30 pKa = 11.84 KK31 pKa = 9.66 RR32 pKa = 11.84 RR33 pKa = 11.84 GAKK36 pKa = 9.63 GYY38 pKa = 10.53 GRR40 pKa = 11.84 ASNLRR45 pKa = 11.84 IRR47 pKa = 11.84 YY48 pKa = 8.37 PLFPDD53 pKa = 3.42 RR54 pKa = 11.84 VFIKK58 pKa = 10.56 LNYY61 pKa = 9.53 AEE63 pKa = 5.4 RR64 pKa = 11.84 INATSTSGGISQYY77 pKa = 10.83 LFRR80 pKa = 11.84 GNSLFDD86 pKa = 4.57 PNFSGTGHH94 pKa = 5.77 QPYY97 pKa = 10.34 GYY99 pKa = 9.61 DD100 pKa = 2.64 QWANMYY106 pKa = 10.35 SSYY109 pKa = 10.41 RR110 pKa = 11.84 VHH112 pKa = 6.78 GSKK115 pKa = 9.25 FTVKK119 pKa = 10.55 LLPITDD125 pKa = 4.13 TNPEE129 pKa = 3.68 IQAAGLMDD137 pKa = 4.29 AVLVAFSGINTLSYY151 pKa = 11.25 VSIDD155 pKa = 3.5 QARR158 pKa = 11.84 EE159 pKa = 3.78 SPYY162 pKa = 10.78 NRR164 pKa = 11.84 HH165 pKa = 5.29 TLLNRR170 pKa = 11.84 NGSRR174 pKa = 11.84 QGYY177 pKa = 9.1 LKK179 pKa = 10.12 MYY181 pKa = 9.28 MSTAKK186 pKa = 9.67 MYY188 pKa = 10.61 GVSKK192 pKa = 11.14 VNIQANDD199 pKa = 3.2 AYY201 pKa = 10.76 AAGTGANPGNQFQWILIVKK220 pKa = 9.81 SADD223 pKa = 3.25 NLVTITALADD233 pKa = 3.52 VKK235 pKa = 9.9 ITYY238 pKa = 10.02 YY239 pKa = 10.85 CEE241 pKa = 3.81 LYY243 pKa = 11.01 NRR245 pKa = 11.84 ISVNQSS251 pKa = 2.3
Molecular weight: 28.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.275
IPC2_protein 10.043
IPC_protein 10.599
Toseland 10.248
ProMoST 10.087
Dawson 10.496
Bjellqvist 10.248
Wikipedia 10.73
Rodwell 10.745
Grimsley 10.599
Solomon 10.526
Lehninger 10.482
Nozaki 10.218
DTASelect 10.248
Thurlkill 10.335
EMBOSS 10.672
Sillero 10.409
Patrickios 10.131
IPC_peptide 10.526
IPC2_peptide 9.151
IPC2.peptide.svr19 8.422
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
542
251
291
271.0
31.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.351 ± 1.469
1.292 ± 0.591
4.797 ± 0.802
4.613 ± 1.998
5.166 ± 0.782
6.089 ± 1.244
1.845 ± 0.166
7.011 ± 0.949
6.642 ± 0.705
7.011 ± 0.106
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.214 ± 0.381
6.089 ± 0.717
3.69 ± 0.069
5.166 ± 0.782
7.196 ± 1.303
7.011 ± 1.161
6.273 ± 0.46
3.321 ± 1.23
1.107 ± 0.205
8.118 ± 0.099
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here