Leucobacter sp. OH1287

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leucobacter; unclassified Leucobacter

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1690 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P1XSB2|A0A3P1XSB2_9MICO tRNA (guanine-N(7)-)-methyltransferase OS=Leucobacter sp. OH1287 OX=2491049 GN=trmB PE=3 SV=1
MM1 pKa = 7.56SKK3 pKa = 10.86YY4 pKa = 8.88LTQYY8 pKa = 9.55EE9 pKa = 4.45LQGSAQLQRR18 pKa = 11.84RR19 pKa = 11.84VAACAASLGVKK30 pKa = 10.39DD31 pKa = 4.54PDD33 pKa = 3.39GFAYY37 pKa = 9.99DD38 pKa = 3.66HH39 pKa = 6.92RR40 pKa = 11.84WRR42 pKa = 11.84LAVTPGWVEE51 pKa = 4.18LYY53 pKa = 10.46DD54 pKa = 4.38AEE56 pKa = 4.32AAQMPEE62 pKa = 4.05PEE64 pKa = 4.18TEE66 pKa = 4.27YY67 pKa = 10.27TAEE70 pKa = 4.89DD71 pKa = 4.21YY72 pKa = 10.03PDD74 pKa = 4.59PSWVIPDD81 pKa = 3.7EE82 pKa = 4.39QILDD86 pKa = 3.65VVQAILAEE94 pKa = 4.04QAAAAGSSTAAPGDD108 pKa = 3.73SLAPGDD114 pKa = 4.43SLAPGDD120 pKa = 4.24GSTPGDD126 pKa = 4.12TPTADD131 pKa = 3.48PGALAGEE138 pKa = 4.26NDD140 pKa = 3.81NPEE143 pKa = 4.18DD144 pKa = 3.56TATAGEE150 pKa = 4.57TPAATAEE157 pKa = 4.04PAEE160 pKa = 4.41PGIAGDD166 pKa = 4.06NADD169 pKa = 3.76ASS171 pKa = 4.04

Molecular weight: 17.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P1XR31|A0A3P1XR31_9MICO Uncharacterized protein OS=Leucobacter sp. OH1287 OX=2491049 GN=EII30_02545 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.74GRR40 pKa = 11.84AKK42 pKa = 10.68LSAA45 pKa = 3.92

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1690

0

1690

559932

32

2163

331.3

35.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.274 ± 0.088

0.633 ± 0.016

5.375 ± 0.042

6.203 ± 0.046

3.303 ± 0.039

8.259 ± 0.055

1.875 ± 0.026

5.189 ± 0.046

3.693 ± 0.045

10.029 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.682 ± 0.022

3.065 ± 0.034

4.779 ± 0.043

3.833 ± 0.039

5.958 ± 0.051

6.023 ± 0.043

6.157 ± 0.043

8.125 ± 0.055

1.289 ± 0.024

2.255 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski