Pelagibacter phage HTVC119P

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Votkovvirus; unclassified Votkovvirus

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y1NTN2|A0A4Y1NTN2_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC119P OX=2283020 GN=P119_gp27 PE=4 SV=1
MM1 pKa = 7.57ANSFLEE7 pKa = 4.11YY8 pKa = 9.86TGNGNTTAFSITFDD22 pKa = 3.78YY23 pKa = 11.26LDD25 pKa = 4.44ASHH28 pKa = 7.46IACTVNGVSTSFTLSNGGATANISPAPANAAAIRR62 pKa = 11.84FTRR65 pKa = 11.84TTSQSTRR72 pKa = 11.84LTDD75 pKa = 3.53YY76 pKa = 11.04VAGSVLKK83 pKa = 10.94EE84 pKa = 3.86EE85 pKa = 5.72DD86 pKa = 4.17LDD88 pKa = 4.11TDD90 pKa = 3.91SKK92 pKa = 11.34QAFFMAQEE100 pKa = 4.71GIEE103 pKa = 4.36TISSKK108 pKa = 10.07MGQSTTNFQFDD119 pKa = 3.99ALNKK123 pKa = 10.07RR124 pKa = 11.84IINVADD130 pKa = 3.7PVDD133 pKa = 3.57NTDD136 pKa = 3.33AVNKK140 pKa = 9.92QFISTNLPNITTVAGISSDD159 pKa = 3.43VTTVAGISSDD169 pKa = 3.42VTAVANNEE177 pKa = 3.7ADD179 pKa = 3.43IDD181 pKa = 4.17LVALNMPSVTTVATNINDD199 pKa = 4.02VITVANDD206 pKa = 3.15LNEE209 pKa = 4.92AISEE213 pKa = 4.21IEE215 pKa = 3.93TAANDD220 pKa = 3.8LNEE223 pKa = 4.38ATSEE227 pKa = 3.83IDD229 pKa = 3.41TVSNNIANVNIVGTNIGNVNTIATANTDD257 pKa = 3.16IQTLADD263 pKa = 4.24LEE265 pKa = 5.04DD266 pKa = 4.01GTVTTNGLSTLAGLNTEE283 pKa = 3.89IQGVYY288 pKa = 10.47NIRR291 pKa = 11.84NNVTNVDD298 pKa = 3.67TNATNVNLVAGQISPTNNVSAVSAVATEE326 pKa = 3.92IGTLGALGTEE336 pKa = 3.91ITNLNNIRR344 pKa = 11.84TDD346 pKa = 2.93ITGVNTISADD356 pKa = 3.41VTGVNNIAPAVTAVNNNSANINAVNANSVNINAVKK391 pKa = 10.75NNEE394 pKa = 3.94ANINAVNANSANINTVAGLNTEE416 pKa = 4.05ITALGASGTVASINTVANNLASVNSFANTYY446 pKa = 10.15LGASATAPTQDD457 pKa = 3.92PDD459 pKa = 4.51GSALDD464 pKa = 4.82LGDD467 pKa = 5.26LYY469 pKa = 11.34FDD471 pKa = 3.95TASDD475 pKa = 3.54TMKK478 pKa = 10.42VYY480 pKa = 10.97SSGGWINAGSAVNGTANRR498 pKa = 11.84FKK500 pKa = 10.31YY501 pKa = 8.64TATASQTTFSGADD514 pKa = 3.29DD515 pKa = 3.9SGNTLAYY522 pKa = 10.3DD523 pKa = 3.29SGYY526 pKa = 11.19ADD528 pKa = 3.57IYY530 pKa = 10.88LNGVKK535 pKa = 9.97LVNGSDD541 pKa = 3.7FTATSGTSIVLASGASANDD560 pKa = 3.21ILEE563 pKa = 4.43VIAYY567 pKa = 7.63GTFTLSNFSITNANDD582 pKa = 3.06VPALGSAGQVLQVNSGATALEE603 pKa = 4.51FGTVDD608 pKa = 4.95LANLNADD615 pKa = 3.68NLTSGTVPSARR626 pKa = 11.84VSGAYY631 pKa = 8.91TGITQTGTLTKK642 pKa = 10.42SGATVTTFNRR652 pKa = 11.84TTSDD656 pKa = 3.34GNILEE661 pKa = 4.32LQKK664 pKa = 10.51DD665 pKa = 3.79TSLSGSVGSVGGNGAFYY682 pKa = 10.4ISGANNVGLQFNNVNDD698 pKa = 4.43EE699 pKa = 4.0IRR701 pKa = 11.84PCNSDD706 pKa = 2.65GSARR710 pKa = 11.84DD711 pKa = 3.49NAIDD715 pKa = 4.2LGNATRR721 pKa = 11.84RR722 pKa = 11.84FKK724 pKa = 10.88DD725 pKa = 3.36LYY727 pKa = 10.77LGGGLYY733 pKa = 10.72VGGTGTANKK742 pKa = 10.15LDD744 pKa = 4.51DD745 pKa = 4.22YY746 pKa = 11.86EE747 pKa = 4.58EE748 pKa = 4.74GTWTPNVNSTSGFTNTNVV766 pKa = 2.86

Molecular weight: 78.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y1NT39|A0A4Y1NT39_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC119P OX=2283020 GN=P119_gp24 PE=4 SV=1
MM1 pKa = 7.35ATPGKK6 pKa = 10.38QYY8 pKa = 10.65ISRR11 pKa = 11.84NLSGRR16 pKa = 11.84VLTRR20 pKa = 11.84GYY22 pKa = 9.01TEE24 pKa = 4.73NGWTYY29 pKa = 11.56LDD31 pKa = 3.27LRR33 pKa = 11.84TRR35 pKa = 11.84KK36 pKa = 9.96SRR38 pKa = 11.84VSEE41 pKa = 3.85RR42 pKa = 11.84WVKK45 pKa = 10.5SSYY48 pKa = 10.54FKK50 pKa = 10.87IMCITQAWQASQHH63 pKa = 4.84RR64 pKa = 11.84AKK66 pKa = 10.5RR67 pKa = 11.84KK68 pKa = 9.12KK69 pKa = 9.98LKK71 pKa = 9.17HH72 pKa = 6.05TITLLQLIKK81 pKa = 10.53LYY83 pKa = 9.98PKK85 pKa = 10.91DD86 pKa = 3.48HH87 pKa = 6.51MCPVFKK93 pKa = 10.49TPLVFGGGLNKK104 pKa = 10.11FSPSIDD110 pKa = 3.37RR111 pKa = 11.84INNLKK116 pKa = 10.69GYY118 pKa = 10.51VKK120 pKa = 10.92GNVQWISSRR129 pKa = 11.84ANTLKK134 pKa = 10.42RR135 pKa = 11.84DD136 pKa = 3.58ATAKK140 pKa = 10.14EE141 pKa = 4.81LYY143 pKa = 9.52TLADD147 pKa = 4.06YY148 pKa = 10.91ISTINKK154 pKa = 8.18TKK156 pKa = 10.32II157 pKa = 3.11

Molecular weight: 18.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

11891

39

1210

220.2

24.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.636 ± 0.433

0.95 ± 0.131

5.937 ± 0.238

6.08 ± 0.404

3.734 ± 0.188

6.459 ± 0.43

1.539 ± 0.186

6.921 ± 0.171

8.712 ± 0.723

8.342 ± 0.341

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.195 ± 0.19

6.383 ± 0.485

3.271 ± 0.227

3.902 ± 0.276

4.045 ± 0.28

6.787 ± 0.463

6.677 ± 0.615

5.777 ± 0.245

1.118 ± 0.142

3.532 ± 0.217
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski