Nocardioides sp. BN140041

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4050 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B1M656|A0A5B1M656_9ACTN Beta-glucosidase OS=Nocardioides sp. BN140041 OX=2607659 GN=F0U47_11055 PE=3 SV=1
MM1 pKa = 7.61KK2 pKa = 9.21NTTRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84TVCGISLLSALSLAVAACGNGSDD32 pKa = 3.96EE33 pKa = 5.49AGDD36 pKa = 3.87EE37 pKa = 4.25NEE39 pKa = 4.34LTFVNYY45 pKa = 10.61GGDD48 pKa = 3.31GMEE51 pKa = 4.09AAKK54 pKa = 10.66AGWLEE59 pKa = 4.26PYY61 pKa = 9.19TEE63 pKa = 4.19KK64 pKa = 10.49TGVTFEE70 pKa = 4.23TDD72 pKa = 3.24EE73 pKa = 5.19PSDD76 pKa = 3.62QAKK79 pKa = 9.51IKK81 pKa = 11.1AMVASGKK88 pKa = 7.35VTWDD92 pKa = 3.27VVDD95 pKa = 4.35IDD97 pKa = 4.13AASAGPEE104 pKa = 4.03CGTLYY109 pKa = 10.67EE110 pKa = 4.56EE111 pKa = 4.96RR112 pKa = 11.84PSDD115 pKa = 3.69FDD117 pKa = 3.46TSEE120 pKa = 3.99LMEE123 pKa = 4.52NEE125 pKa = 3.79ITDD128 pKa = 3.62DD129 pKa = 4.11CMIPIINQTVALVYY143 pKa = 10.36NAEE146 pKa = 4.44LYY148 pKa = 10.94GDD150 pKa = 4.25NPPTSITDD158 pKa = 3.75FFNPEE163 pKa = 3.77FEE165 pKa = 4.33GQRR168 pKa = 11.84ITFSYY173 pKa = 7.3WTGSAEE179 pKa = 4.15PLALAAGIPAEE190 pKa = 4.4DD191 pKa = 4.69VYY193 pKa = 11.19PIDD196 pKa = 3.46WDD198 pKa = 3.88EE199 pKa = 4.74LKK201 pKa = 11.0SAVDD205 pKa = 3.58SLGSDD210 pKa = 3.47LTFQSTLDD218 pKa = 3.45QQVQSLEE225 pKa = 4.07SGNFGMCLCYY235 pKa = 9.89TGRR238 pKa = 11.84SALAAQNGADD248 pKa = 4.66IGVVWDD254 pKa = 4.28KK255 pKa = 11.58VWVGWDD261 pKa = 3.11GLYY264 pKa = 10.31VVKK267 pKa = 10.68DD268 pKa = 3.65SPRR271 pKa = 11.84EE272 pKa = 4.01DD273 pKa = 3.51IAWDD277 pKa = 4.17FVQYY281 pKa = 10.91LATSEE286 pKa = 4.31GQNGYY291 pKa = 9.39YY292 pKa = 10.02EE293 pKa = 4.66HH294 pKa = 7.26LPYY297 pKa = 10.89SPTTTGAPPDD307 pKa = 3.56VPEE310 pKa = 4.02EE311 pKa = 4.07FKK313 pKa = 11.19PFMLSFNEE321 pKa = 4.28GKK323 pKa = 9.9VQEE326 pKa = 4.35TSYY329 pKa = 11.39FDD331 pKa = 2.96AGYY334 pKa = 9.52WGDD337 pKa = 3.75QGATAADD344 pKa = 3.69EE345 pKa = 4.42WTALTAGG352 pKa = 3.85

Molecular weight: 38.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B1LZD7|A0A5B1LZD7_9ACTN Probable membrane transporter protein OS=Nocardioides sp. BN140041 OX=2607659 GN=F0U47_17060 PE=3 SV=1
MM1 pKa = 7.47RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84ARR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84PPRR12 pKa = 11.84PPARR16 pKa = 11.84WTPPRR21 pKa = 11.84PWRR24 pKa = 11.84APRR27 pKa = 11.84PPSRR31 pKa = 11.84RR32 pKa = 11.84CPRR35 pKa = 11.84SGRR38 pKa = 11.84PARR41 pKa = 11.84PWSPTPTRR49 pKa = 11.84RR50 pKa = 11.84SRR52 pKa = 11.84PGRR55 pKa = 11.84RR56 pKa = 11.84GGRR59 pKa = 11.84GCC61 pKa = 4.61

Molecular weight: 7.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4050

0

4050

1263282

33

2527

311.9

33.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.816 ± 0.049

0.772 ± 0.011

6.712 ± 0.037

6.086 ± 0.035

2.817 ± 0.018

9.162 ± 0.036

2.165 ± 0.02

3.596 ± 0.024

2.075 ± 0.027

10.105 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.804 ± 0.016

1.808 ± 0.022

5.673 ± 0.03

2.713 ± 0.021

7.696 ± 0.046

5.056 ± 0.022

5.961 ± 0.035

9.399 ± 0.038

1.527 ± 0.016

2.056 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski