Helminthosporium victoriae virus-190S (Hv190SV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; Victorivirus

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|O57044|RDRP_HV19S Probable RNA-directed RNA polymerase OS=Helminthosporium victoriae virus-190S OX=45237 PE=3 SV=2
MM1 pKa = 7.23SHH3 pKa = 4.89TTITNFLAGVIARR16 pKa = 11.84PQGGNITSDD25 pKa = 3.19EE26 pKa = 4.06TFRR29 pKa = 11.84RR30 pKa = 11.84YY31 pKa = 8.57RR32 pKa = 11.84TIVRR36 pKa = 11.84TSATIGGNEE45 pKa = 4.31DD46 pKa = 3.25SRR48 pKa = 11.84TTSIFHH54 pKa = 6.62EE55 pKa = 4.35IGRR58 pKa = 11.84AVNTKK63 pKa = 9.83GKK65 pKa = 9.31ALAVAGMEE73 pKa = 4.16APLVEE78 pKa = 4.61ASYY81 pKa = 8.44PTNAVLVEE89 pKa = 4.21DD90 pKa = 5.6FIGLAKK96 pKa = 10.54KK97 pKa = 8.47YY98 pKa = 8.7TNFSATFEE106 pKa = 4.19YY107 pKa = 10.9SSLAGVVEE115 pKa = 4.37RR116 pKa = 11.84LARR119 pKa = 11.84GLAACSVFEE128 pKa = 5.12DD129 pKa = 3.95VTSTDD134 pKa = 3.2LRR136 pKa = 11.84GNNPLAVHH144 pKa = 7.08ALATYY149 pKa = 9.45DD150 pKa = 3.83GPVNSLTSAVFIPRR164 pKa = 11.84LVNNALTGDD173 pKa = 4.13VFAVLCNCVAGEE185 pKa = 4.33GGTVVTDD192 pKa = 4.36TIEE195 pKa = 4.56LDD197 pKa = 3.3ANTRR201 pKa = 11.84QPIVPEE207 pKa = 4.02VGPLGVPGAIVDD219 pKa = 3.99ALRR222 pKa = 11.84LLGSNMIASDD232 pKa = 3.74QGPLFALALTRR243 pKa = 11.84GIHH246 pKa = 5.8RR247 pKa = 11.84VLSVVGHH254 pKa = 5.44TDD256 pKa = 2.38EE257 pKa = 5.21GGIVRR262 pKa = 11.84DD263 pKa = 3.84LLRR266 pKa = 11.84CGGFGLPFGGIHH278 pKa = 6.37YY279 pKa = 10.51GLEE282 pKa = 4.34EE283 pKa = 4.27YY284 pKa = 10.7SGLPALQFNSAAATAAYY301 pKa = 10.28VDD303 pKa = 5.29GIALVTAAVVAHH315 pKa = 7.11ADD317 pKa = 3.11PGEE320 pKa = 4.32RR321 pKa = 11.84YY322 pKa = 9.71NGEE325 pKa = 3.78WFPTFFDD332 pKa = 4.04GTTHH336 pKa = 7.16ADD338 pKa = 3.09TMRR341 pKa = 11.84RR342 pKa = 11.84SGDD345 pKa = 3.61STEE348 pKa = 4.21GTAAMADD355 pKa = 3.83RR356 pKa = 11.84NRR358 pKa = 11.84AQLLARR364 pKa = 11.84QQLFWRR370 pKa = 11.84PYY372 pKa = 7.52ITALGACFSTAGDD385 pKa = 3.2ISVAEE390 pKa = 4.33RR391 pKa = 11.84FQCAASHH398 pKa = 6.46SLGADD403 pKa = 3.36PRR405 pKa = 11.84HH406 pKa = 5.85LRR408 pKa = 11.84LPSVAPYY415 pKa = 9.79FWIEE419 pKa = 3.72PTGLIPHH426 pKa = 7.31DD427 pKa = 3.86FLGSVAEE434 pKa = 4.25EE435 pKa = 4.06EE436 pKa = 5.1GFASYY441 pKa = 10.41CWRR444 pKa = 11.84DD445 pKa = 3.56TTRR448 pKa = 11.84TRR450 pKa = 11.84PAWDD454 pKa = 3.85SIVLSGARR462 pKa = 11.84DD463 pKa = 3.77TTFSAYY469 pKa = 10.01HH470 pKa = 6.39IRR472 pKa = 11.84MKK474 pKa = 10.49GARR477 pKa = 11.84TAWFLAHH484 pKa = 7.03WLGHH488 pKa = 5.84PEE490 pKa = 4.03NGLGATRR497 pKa = 11.84VRR499 pKa = 11.84QLDD502 pKa = 3.91PNAVLHH508 pKa = 6.43PGPCEE513 pKa = 3.74GNEE516 pKa = 3.89QVRR519 pKa = 11.84DD520 pKa = 3.53RR521 pKa = 11.84VEE523 pKa = 4.62ADD525 pKa = 4.07LPLTDD530 pKa = 3.83YY531 pKa = 10.94LWRR534 pKa = 11.84RR535 pKa = 11.84GQSPFPAAGEE545 pKa = 4.04LLNLTSEE552 pKa = 3.76WGILFRR558 pKa = 11.84HH559 pKa = 5.35VTFTDD564 pKa = 4.01DD565 pKa = 3.5GDD567 pKa = 4.51LNPEE571 pKa = 4.29HH572 pKa = 6.96LPAAHH577 pKa = 6.69EE578 pKa = 4.21MADD581 pKa = 3.56TTVTMTVGRR590 pKa = 11.84PIGIAPGRR598 pKa = 11.84SNAGDD603 pKa = 3.45NQARR607 pKa = 11.84RR608 pKa = 11.84ARR610 pKa = 11.84TRR612 pKa = 11.84ASVEE616 pKa = 3.42LSAASRR622 pKa = 11.84RR623 pKa = 11.84ARR625 pKa = 11.84VFGRR629 pKa = 11.84PDD631 pKa = 3.24VGEE634 pKa = 4.23MPTLTSAPAPIPASPAYY651 pKa = 10.01DD652 pKa = 3.34GNRR655 pKa = 11.84GGEE658 pKa = 4.05AGGVTGRR665 pKa = 11.84GNNRR669 pKa = 11.84SAAPGHH675 pKa = 6.16ASWSEE680 pKa = 3.84RR681 pKa = 11.84QADD684 pKa = 3.98GVPVNVTPHH693 pKa = 6.25HH694 pKa = 5.92NALRR698 pKa = 11.84APPFPRR704 pKa = 11.84QQGALGGGGNVPLPPAPGAAPPPPPGPPNGPPAGPPPSDD743 pKa = 4.45DD744 pKa = 3.71GSSNPAAPVPTAIHH758 pKa = 6.69APPAAAQADD767 pKa = 4.26RR768 pKa = 11.84AEE770 pKa = 4.4GQQ772 pKa = 3.15

Molecular weight: 81.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O57044|RDRP_HV19S Probable RNA-directed RNA polymerase OS=Helminthosporium victoriae virus-190S OX=45237 PE=3 SV=2
MM1 pKa = 7.96SDD3 pKa = 3.1PQEE6 pKa = 3.8RR7 pKa = 11.84SKK9 pKa = 11.34AYY11 pKa = 9.94GLLGEE16 pKa = 4.57RR17 pKa = 11.84LYY19 pKa = 11.36AVASANSHH27 pKa = 5.34MLAGYY32 pKa = 10.42DD33 pKa = 3.81SLDD36 pKa = 3.22FTARR40 pKa = 11.84LVRR43 pKa = 11.84LTGEE47 pKa = 3.93ATALKK52 pKa = 10.39AVDD55 pKa = 4.32PLLPCAVSLLFMDD68 pKa = 6.31FPLQLPCTPEE78 pKa = 3.42EE79 pKa = 3.92TLRR82 pKa = 11.84LVRR85 pKa = 11.84RR86 pKa = 11.84AYY88 pKa = 10.41DD89 pKa = 3.57PNTLEE94 pKa = 4.04EE95 pKa = 4.09VDD97 pKa = 3.89YY98 pKa = 10.23STLSGYY104 pKa = 7.97ATQFARR110 pKa = 11.84VKK112 pKa = 9.46GQRR115 pKa = 11.84GRR117 pKa = 11.84WRR119 pKa = 11.84HH120 pKa = 5.7LGHH123 pKa = 7.31LVCNDD128 pKa = 2.56KK129 pKa = 11.1AFRR132 pKa = 11.84EE133 pKa = 4.38RR134 pKa = 11.84YY135 pKa = 8.97FPKK138 pKa = 10.22KK139 pKa = 9.47KK140 pKa = 9.78HH141 pKa = 5.25AAAAIKK147 pKa = 9.65TNIRR151 pKa = 11.84LGPLARR157 pKa = 11.84AWAARR162 pKa = 11.84YY163 pKa = 9.14GLAALGSHH171 pKa = 7.08LAYY174 pKa = 9.66MVGMPNDD181 pKa = 3.88RR182 pKa = 11.84ACATLLLAQTYY193 pKa = 8.78KK194 pKa = 11.02ARR196 pKa = 11.84FGSEE200 pKa = 3.6GVAWAIASVRR210 pKa = 11.84QPEE213 pKa = 4.2NAKK216 pKa = 10.47GLSNALKK223 pKa = 10.57ALGSNTSEE231 pKa = 4.0PGALFVEE238 pKa = 4.86ANTLQGRR245 pKa = 11.84YY246 pKa = 9.91DD247 pKa = 3.37RR248 pKa = 11.84TLDD251 pKa = 3.26MDD253 pKa = 4.12HH254 pKa = 6.69EE255 pKa = 4.73VEE257 pKa = 4.71SRR259 pKa = 11.84CSPAAIADD267 pKa = 3.62QVIPYY272 pKa = 9.05TDD274 pKa = 3.53EE275 pKa = 4.61LGACIDD281 pKa = 4.91FILDD285 pKa = 3.61TEE287 pKa = 4.73LGGDD291 pKa = 3.88TIEE294 pKa = 6.26LPDD297 pKa = 4.05EE298 pKa = 4.62DD299 pKa = 4.5EE300 pKa = 4.09WWTSRR305 pKa = 11.84WLWCVNGSQNALSDD319 pKa = 3.44KK320 pKa = 11.21ALGIKK325 pKa = 9.88NKK327 pKa = 10.12SGQRR331 pKa = 11.84YY332 pKa = 8.22RR333 pKa = 11.84RR334 pKa = 11.84MAAEE338 pKa = 4.01EE339 pKa = 4.37VNNNPVPAWNGHH351 pKa = 4.63TSVSPSVKK359 pKa = 10.42LEE361 pKa = 3.85NGKK364 pKa = 9.89DD365 pKa = 3.23RR366 pKa = 11.84AIFACDD372 pKa = 3.05TRR374 pKa = 11.84SYY376 pKa = 11.07FAFTYY381 pKa = 9.55WLTPIEE387 pKa = 4.46KK388 pKa = 8.58KK389 pKa = 9.44WRR391 pKa = 11.84GARR394 pKa = 11.84VILNPGEE401 pKa = 4.14GGLYY405 pKa = 8.56GTARR409 pKa = 11.84RR410 pKa = 11.84IRR412 pKa = 11.84GSQTSGGVNLMLDD425 pKa = 3.53YY426 pKa = 11.59DD427 pKa = 4.27NFNSQHH433 pKa = 6.26SNEE436 pKa = 4.15TMAALYY442 pKa = 9.4EE443 pKa = 4.29KK444 pKa = 10.73ALSRR448 pKa = 11.84TNAPAYY454 pKa = 9.99LKK456 pKa = 10.51KK457 pKa = 10.55AVAASVEE464 pKa = 4.3STYY467 pKa = 10.92IHH469 pKa = 6.15YY470 pKa = 10.38KK471 pKa = 9.92GRR473 pKa = 11.84DD474 pKa = 3.08RR475 pKa = 11.84HH476 pKa = 5.76VLGTLMSGHH485 pKa = 7.19RR486 pKa = 11.84ATTFTNSVLNAAYY499 pKa = 8.95ICYY502 pKa = 10.2AVGIPAFKK510 pKa = 10.75RR511 pKa = 11.84MISLHH516 pKa = 6.61AGDD519 pKa = 5.45DD520 pKa = 3.79VYY522 pKa = 11.72LRR524 pKa = 11.84LPTLADD530 pKa = 3.61CATTLNNTKK539 pKa = 10.38RR540 pKa = 11.84VGCRR544 pKa = 11.84MNPTKK549 pKa = 10.55QSIGYY554 pKa = 7.82TGAEE558 pKa = 4.29FLRR561 pKa = 11.84LGINKK566 pKa = 9.61SYY568 pKa = 10.93AIGYY572 pKa = 7.5LCRR575 pKa = 11.84AIASLVSGSWTSLDD589 pKa = 3.4EE590 pKa = 4.23LQPLNALNGAIVQTRR605 pKa = 11.84SCLNRR610 pKa = 11.84GAATGLPEE618 pKa = 5.6LISASFVGLRR628 pKa = 11.84GFKK631 pKa = 10.4RR632 pKa = 11.84RR633 pKa = 11.84DD634 pKa = 3.39LLEE637 pKa = 4.51LLTGVATIKK646 pKa = 10.27PGPVYY651 pKa = 10.29TSSCVIRR658 pKa = 11.84EE659 pKa = 4.17YY660 pKa = 11.04VVEE663 pKa = 4.28QPPPPQFDD671 pKa = 3.89VPPGAGMHH679 pKa = 5.84ATMSYY684 pKa = 10.24LARR687 pKa = 11.84HH688 pKa = 4.9TTLVEE693 pKa = 3.96AQALEE698 pKa = 4.08IARR701 pKa = 11.84PAIKK705 pKa = 10.43SLMLSSSYY713 pKa = 11.11GKK715 pKa = 10.27AGPGAQTRR723 pKa = 11.84PHH725 pKa = 7.54VPMPKK730 pKa = 9.85LRR732 pKa = 11.84RR733 pKa = 11.84QPPRR737 pKa = 11.84VAVGFSMAHH746 pKa = 6.23EE747 pKa = 4.02LTGRR751 pKa = 11.84GVKK754 pKa = 9.67EE755 pKa = 4.3GCLSGHH761 pKa = 6.32PLLRR765 pKa = 11.84LFEE768 pKa = 4.06QRR770 pKa = 11.84LTDD773 pKa = 4.05DD774 pKa = 4.21DD775 pKa = 4.78LRR777 pKa = 11.84ALVALVGGNTSAKK790 pKa = 10.14DD791 pKa = 3.11IRR793 pKa = 11.84AEE795 pKa = 3.89AFGAEE800 pKa = 4.42SSSSTIMGILPHH812 pKa = 6.92SDD814 pKa = 2.3ASNYY818 pKa = 9.62CKK820 pKa = 9.86RR821 pKa = 11.84TRR823 pKa = 11.84NGNIIVPYY831 pKa = 9.8HH832 pKa = 5.86IRR834 pKa = 11.84SS835 pKa = 3.46

Molecular weight: 91.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1607

772

835

803.5

86.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.321 ± 0.688

1.493 ± 0.308

4.729 ± 0.305

4.729 ± 0.045

3.174 ± 0.393

9.272 ± 0.91

2.551 ± 0.201

3.796 ± 0.114

2.427 ± 1.199

9.334 ± 1.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.68 ± 0.347

4.231 ± 0.058

6.845 ± 1.149

2.365 ± 0.11

7.467 ± 0.057

6.472 ± 0.521

6.783 ± 0.405

6.036 ± 0.472

1.307 ± 0.008

2.987 ± 0.704
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski