Fibroporia radiculosa

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Polyporales incertae sedis; Fibroporia

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9251 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|J4GN21|J4GN21_9APHY Uncharacterized protein OS=Fibroporia radiculosa OX=599839 GN=FIBRA_02709 PE=4 SV=1
MM1 pKa = 7.2GAYY4 pKa = 9.41YY5 pKa = 10.61DD6 pKa = 3.77EE7 pKa = 5.18VEE9 pKa = 4.36IEE11 pKa = 4.08DD12 pKa = 4.05MAWDD16 pKa = 3.59EE17 pKa = 4.61DD18 pKa = 3.38KK19 pKa = 10.93RR20 pKa = 11.84IYY22 pKa = 10.32HH23 pKa = 5.62YY24 pKa = 9.88PCPCGDD30 pKa = 3.27RR31 pKa = 11.84FEE33 pKa = 4.69ISRR36 pKa = 11.84KK37 pKa = 8.98QLANYY42 pKa = 9.44EE43 pKa = 4.73DD44 pKa = 4.57IATCPSCSLVIRR56 pKa = 11.84VIYY59 pKa = 10.38DD60 pKa = 3.27PLDD63 pKa = 4.13YY64 pKa = 10.93EE65 pKa = 5.51DD66 pKa = 5.26EE67 pKa = 4.91PPDD70 pKa = 4.02EE71 pKa = 5.23DD72 pKa = 5.71DD73 pKa = 4.08EE74 pKa = 5.15GAVSDD79 pKa = 4.5EE80 pKa = 4.72EE81 pKa = 4.72SDD83 pKa = 4.56EE84 pKa = 4.78DD85 pKa = 5.37DD86 pKa = 4.12SDD88 pKa = 4.32SGDD91 pKa = 3.5EE92 pKa = 4.09RR93 pKa = 11.84FEE95 pKa = 4.47DD96 pKa = 4.15ALDD99 pKa = 3.98RR100 pKa = 11.84LHH102 pKa = 7.12LADD105 pKa = 4.73EE106 pKa = 4.8RR107 pKa = 11.84PLVIAVGAA115 pKa = 3.99

Molecular weight:
13.16 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|J4HWQ2|J4HWQ2_9APHY Uncharacterized protein OS=Fibroporia radiculosa OX=599839 GN=FIBRA_04761 PE=4 SV=1
MM1 pKa = 7.42SVPRR5 pKa = 11.84LPAIGRR11 pKa = 11.84CSNPCRR17 pKa = 11.84SPWRR21 pKa = 11.84TRR23 pKa = 11.84IQPSLSSPALAALLRR38 pKa = 11.84TRR40 pKa = 11.84TRR42 pKa = 11.84TLTSLPGSSTSSPSSPPPKK61 pKa = 10.49LPMTKK66 pKa = 9.74PLSSPILPQSMPPDD80 pKa = 4.4PDD82 pKa = 4.24FEE84 pKa = 4.71PSPPNSALLLFCLSTGLVDD103 pKa = 4.07SLTFAVSGVWCAFVTGTTVQLGMDD127 pKa = 4.54LPALLAHH134 pKa = 6.6LPALFAHH141 pKa = 7.15LPTSTTEE148 pKa = 3.82LSIATSQFLSSGSSEE163 pKa = 3.92RR164 pKa = 11.84LSALGGFLLGGLSAARR180 pKa = 11.84FPEE183 pKa = 4.95AISPARR189 pKa = 11.84STAFQSALLVMASVLVLTTTPAGAEE214 pKa = 4.01YY215 pKa = 10.57LSYY218 pKa = 11.4SHH220 pKa = 6.89LTLALISSSMAIQAVLVQRR239 pKa = 11.84FATPYY244 pKa = 7.71ATSVAWTSVWLSAVAPGSPTKK265 pKa = 9.72RR266 pKa = 11.84WKK268 pKa = 10.74RR269 pKa = 11.84FVGLGAVMSGAALGASLLVFGVDD292 pKa = 3.7DD293 pKa = 3.91EE294 pKa = 4.61EE295 pKa = 4.6KK296 pKa = 10.54ARR298 pKa = 11.84EE299 pKa = 4.07EE300 pKa = 4.0MVHH303 pKa = 5.46RR304 pKa = 11.84VGWGLAVAALVKK316 pKa = 10.69AIVAGMFWRR325 pKa = 11.84RR326 pKa = 11.84GRR328 pKa = 11.84RR329 pKa = 11.84RR330 pKa = 11.84GIQLL334 pKa = 3.32

Molecular weight:
35.15 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9251

0

9251

4412171

8

5034

476.9

52.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.808 ± 0.019

1.143 ± 0.009

5.693 ± 0.019

5.876 ± 0.024

3.635 ± 0.015

6.46 ± 0.023

2.523 ± 0.011

4.801 ± 0.018

4.169 ± 0.022

9.331 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.147 ± 0.008

3.223 ± 0.012

6.548 ± 0.032

3.719 ± 0.015

6.392 ± 0.024

8.937 ± 0.034

5.954 ± 0.018

6.58 ± 0.018

1.396 ± 0.009

2.665 ± 0.013

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski