Fibroporia radiculosa
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9251 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J4GN21|J4GN21_9APHY Uncharacterized protein OS=Fibroporia radiculosa OX=599839 GN=FIBRA_02709 PE=4 SV=1
MM1 pKa = 7.2 GAYY4 pKa = 9.41 YY5 pKa = 10.61 DD6 pKa = 3.77 EE7 pKa = 5.18 VEE9 pKa = 4.36 IEE11 pKa = 4.08 DD12 pKa = 4.05 MAWDD16 pKa = 3.59 EE17 pKa = 4.61 DD18 pKa = 3.38 KK19 pKa = 10.93 RR20 pKa = 11.84 IYY22 pKa = 10.32 HH23 pKa = 5.62 YY24 pKa = 9.88 PCPCGDD30 pKa = 3.27 RR31 pKa = 11.84 FEE33 pKa = 4.69 ISRR36 pKa = 11.84 KK37 pKa = 8.98 QLANYY42 pKa = 9.44 EE43 pKa = 4.73 DD44 pKa = 4.57 IATCPSCSLVIRR56 pKa = 11.84 VIYY59 pKa = 10.38 DD60 pKa = 3.27 PLDD63 pKa = 4.13 YY64 pKa = 10.93 EE65 pKa = 5.51 DD66 pKa = 5.26 EE67 pKa = 4.91 PPDD70 pKa = 4.02 EE71 pKa = 5.23 DD72 pKa = 5.71 DD73 pKa = 4.08 EE74 pKa = 5.15 GAVSDD79 pKa = 4.5 EE80 pKa = 4.72 EE81 pKa = 4.72 SDD83 pKa = 4.56 EE84 pKa = 4.78 DD85 pKa = 5.37 DD86 pKa = 4.12 SDD88 pKa = 4.32 SGDD91 pKa = 3.5 EE92 pKa = 4.09 RR93 pKa = 11.84 FEE95 pKa = 4.47 DD96 pKa = 4.15 ALDD99 pKa = 3.98 RR100 pKa = 11.84 LHH102 pKa = 7.12 LADD105 pKa = 4.73 EE106 pKa = 4.8 RR107 pKa = 11.84 PLVIAVGAA115 pKa = 3.99
Molecular weight: 13.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.706
IPC_protein 3.706
Toseland 3.49
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.63
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.643
Nozaki 3.808
DTASelect 4.037
Thurlkill 3.541
EMBOSS 3.63
Sillero 3.821
Patrickios 1.062
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|J4HWQ2|J4HWQ2_9APHY Uncharacterized protein OS=Fibroporia radiculosa OX=599839 GN=FIBRA_04761 PE=4 SV=1
MM1 pKa = 7.42 SVPRR5 pKa = 11.84 LPAIGRR11 pKa = 11.84 CSNPCRR17 pKa = 11.84 SPWRR21 pKa = 11.84 TRR23 pKa = 11.84 IQPSLSSPALAALLRR38 pKa = 11.84 TRR40 pKa = 11.84 TRR42 pKa = 11.84 TLTSLPGSSTSSPSSPPPKK61 pKa = 10.49 LPMTKK66 pKa = 9.74 PLSSPILPQSMPPDD80 pKa = 4.4 PDD82 pKa = 4.24 FEE84 pKa = 4.71 PSPPNSALLLFCLSTGLVDD103 pKa = 4.07 SLTFAVSGVWCAFVTGTTVQLGMDD127 pKa = 4.54 LPALLAHH134 pKa = 6.6 LPALFAHH141 pKa = 7.15 LPTSTTEE148 pKa = 3.82 LSIATSQFLSSGSSEE163 pKa = 3.92 RR164 pKa = 11.84 LSALGGFLLGGLSAARR180 pKa = 11.84 FPEE183 pKa = 4.95 AISPARR189 pKa = 11.84 STAFQSALLVMASVLVLTTTPAGAEE214 pKa = 4.01 YY215 pKa = 10.57 LSYY218 pKa = 11.4 SHH220 pKa = 6.89 LTLALISSSMAIQAVLVQRR239 pKa = 11.84 FATPYY244 pKa = 7.71 ATSVAWTSVWLSAVAPGSPTKK265 pKa = 9.72 RR266 pKa = 11.84 WKK268 pKa = 10.74 RR269 pKa = 11.84 FVGLGAVMSGAALGASLLVFGVDD292 pKa = 3.7 DD293 pKa = 3.91 EE294 pKa = 4.61 EE295 pKa = 4.6 KK296 pKa = 10.54 ARR298 pKa = 11.84 EE299 pKa = 4.07 EE300 pKa = 4.0 MVHH303 pKa = 5.46 RR304 pKa = 11.84 VGWGLAVAALVKK316 pKa = 10.69 AIVAGMFWRR325 pKa = 11.84 RR326 pKa = 11.84 GRR328 pKa = 11.84 RR329 pKa = 11.84 RR330 pKa = 11.84 GIQLL334 pKa = 3.32
Molecular weight: 35.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.487
IPC_protein 10.335
Toseland 10.599
ProMoST 10.335
Dawson 10.687
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 10.804
Grimsley 10.745
Solomon 10.833
Lehninger 10.804
Nozaki 10.613
DTASelect 10.423
Thurlkill 10.599
EMBOSS 11.008
Sillero 10.628
Patrickios 10.54
IPC_peptide 10.833
IPC2_peptide 9.692
IPC2.peptide.svr19 8.611
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9251
0
9251
4412171
8
5034
476.9
52.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.808 ± 0.019
1.143 ± 0.009
5.693 ± 0.019
5.876 ± 0.024
3.635 ± 0.015
6.46 ± 0.023
2.523 ± 0.011
4.801 ± 0.018
4.169 ± 0.022
9.331 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.147 ± 0.008
3.223 ± 0.012
6.548 ± 0.032
3.719 ± 0.015
6.392 ± 0.024
8.937 ± 0.034
5.954 ± 0.018
6.58 ± 0.018
1.396 ± 0.009
2.665 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here