[Polyangium] brachysporum
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5541 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0G3BZ90|A0A0G3BZ90_9BURK Uncharacterized protein OS=[Polyangium] brachysporum OX=413882 GN=AAW51_5119 PE=4 SV=1
MM1 pKa = 7.22 NPISEE6 pKa = 4.83 TYY8 pKa = 10.16 LHH10 pKa = 6.17 PTIPTAQPYY19 pKa = 9.66 GAGAPTDD26 pKa = 3.9 PTQLLAQAQNLLEE39 pKa = 4.13 NLEE42 pKa = 4.19 NLVEE46 pKa = 4.14 QLAGAPLDD54 pKa = 3.66 QQDD57 pKa = 4.67 PYY59 pKa = 11.52 GGQQPYY65 pKa = 10.65 GGQQPYY71 pKa = 10.65 GGQQPYY77 pKa = 10.66 GGQQQQFDD85 pKa = 3.84 GGQFQQQPQYY95 pKa = 10.24 QTGQYY100 pKa = 10.03 PNSQPPAGPGPTGQGTAADD119 pKa = 4.29 DD120 pKa = 4.87 GYY122 pKa = 11.74 GAGQGLRR129 pKa = 11.84 DD130 pKa = 3.81 VKK132 pKa = 10.92 INTAAGGEE140 pKa = 4.11 ALHH143 pKa = 6.41 LQADD147 pKa = 4.29 SQGNLYY153 pKa = 10.29 NGSGNHH159 pKa = 5.84 VGVMGQDD166 pKa = 3.42 GSITLNSGATKK177 pKa = 10.0 EE178 pKa = 4.04 RR179 pKa = 11.84 EE180 pKa = 3.85 RR181 pKa = 11.84 LEE183 pKa = 4.44 TGDD186 pKa = 3.33 QGIFKK191 pKa = 10.71 AFGGPDD197 pKa = 3.32 EE198 pKa = 5.76 AEE200 pKa = 4.28 GDD202 pKa = 3.82 DD203 pKa = 4.05 GRR205 pKa = 11.84 DD206 pKa = 4.01 GSVTWDD212 pKa = 3.23 ANQVTVSAGDD222 pKa = 3.78 LNQEE226 pKa = 3.81 NDD228 pKa = 3.2 FF229 pKa = 4.31
Molecular weight: 23.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.821
IPC_protein 3.808
Toseland 3.592
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.503
Solomon 3.795
Lehninger 3.745
Nozaki 3.923
DTASelect 4.151
Thurlkill 3.656
EMBOSS 3.745
Sillero 3.935
Patrickios 0.744
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.828
Protein with the highest isoelectric point:
>tr|A0A0G3BWQ6|A0A0G3BWQ6_9BURK GntR family transcriptional regulator OS=[Polyangium] brachysporum OX=413882 GN=AAW51_5100 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.25 QPSKK9 pKa = 8.87 VRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.65 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.86 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 KK29 pKa = 8.96 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5541
0
5541
1876769
33
10822
338.7
36.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.794 ± 0.055
0.932 ± 0.012
5.341 ± 0.029
5.518 ± 0.033
3.262 ± 0.022
8.358 ± 0.033
2.428 ± 0.017
3.555 ± 0.023
2.711 ± 0.031
10.971 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.091 ± 0.016
2.3 ± 0.021
5.563 ± 0.027
4.268 ± 0.026
7.665 ± 0.038
5.289 ± 0.028
5.18 ± 0.045
7.924 ± 0.031
1.558 ± 0.016
2.293 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here