Vogesella sp. EB
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3143 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0J5G365|A0A0J5G365_9NEIS Glutamate-1-semialdehyde 2 1-aminomutase OS=Vogesella sp. EB OX=1526735 GN=hemL PE=3 SV=1
MM1 pKa = 6.19 QTWMCLICGFIYY13 pKa = 10.63 DD14 pKa = 4.06 EE15 pKa = 4.36 AAGRR19 pKa = 11.84 PEE21 pKa = 5.18 DD22 pKa = 4.91 GIPPGTKK29 pKa = 9.09 WEE31 pKa = 4.15 DD32 pKa = 3.3 VPMNWTCPDD41 pKa = 3.22 CDD43 pKa = 3.45 ARR45 pKa = 11.84 KK46 pKa = 10.36 DD47 pKa = 3.96 DD48 pKa = 4.12 FEE50 pKa = 4.42 MMAFF54 pKa = 3.93
Molecular weight: 6.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.923
IPC_protein 3.821
Toseland 3.617
ProMoST 4.012
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.757
Nozaki 3.961
DTASelect 4.164
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.948
Patrickios 1.914
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A0J5GD06|A0A0J5GD06_9NEIS Uncharacterized protein OS=Vogesella sp. EB OX=1526735 GN=ACG97_15320 PE=4 SV=1
MM1 pKa = 7.82 KK2 pKa = 10.46 LRR4 pKa = 11.84 LQRR7 pKa = 11.84 MLWLVAGWLCLLLALIGALLPVMPTTVFVLMAAACFGRR45 pKa = 11.84 SSPALLHH52 pKa = 6.29 WLHH55 pKa = 6.04 HH56 pKa = 6.27 HH57 pKa = 6.81 PRR59 pKa = 11.84 LGPPLLQWQRR69 pKa = 11.84 YY70 pKa = 8.01 HH71 pKa = 7.93 GMTARR76 pKa = 11.84 TKK78 pKa = 10.2 AVALGTVALSFSFSIYY94 pKa = 9.9 ATLQQPWLTALLLLLWAALSLWMWRR119 pKa = 11.84 LPTIPATSACCAMLDD134 pKa = 4.0 SKK136 pKa = 11.57 SS137 pKa = 3.33
Molecular weight: 15.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.75
IPC_protein 10.613
Toseland 10.672
ProMoST 10.409
Dawson 10.774
Bjellqvist 10.54
Wikipedia 11.008
Rodwell 10.965
Grimsley 10.833
Solomon 10.906
Lehninger 10.877
Nozaki 10.716
DTASelect 10.511
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.73
Patrickios 10.774
IPC_peptide 10.906
IPC2_peptide 9.984
IPC2.peptide.svr19 8.364
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3143
0
3143
978495
42
2924
311.3
33.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.324 ± 0.065
0.987 ± 0.016
5.377 ± 0.035
5.249 ± 0.042
3.55 ± 0.028
7.882 ± 0.038
2.328 ± 0.021
4.745 ± 0.034
3.455 ± 0.039
11.818 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.434 ± 0.022
2.824 ± 0.027
4.745 ± 0.033
4.665 ± 0.041
6.407 ± 0.042
5.461 ± 0.032
4.686 ± 0.029
7.237 ± 0.041
1.373 ± 0.019
2.455 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here