Amino acid dipepetide frequency for Tepidibacillus decaturensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.751AlaAla: 4.751 ± 0.091
0.357AlaCys: 0.357 ± 0.023
2.98AlaAsp: 2.98 ± 0.067
4.312AlaGlu: 4.312 ± 0.085
2.877AlaPhe: 2.877 ± 0.066
4.653AlaGly: 4.653 ± 0.085
1.185AlaHis: 1.185 ± 0.043
6.276AlaIle: 6.276 ± 0.11
5.316AlaLys: 5.316 ± 0.093
6.68AlaLeu: 6.68 ± 0.108
1.952AlaMet: 1.952 ± 0.057
2.794AlaAsn: 2.794 ± 0.054
1.786AlaPro: 1.786 ± 0.049
2.069AlaGln: 2.069 ± 0.062
2.488AlaArg: 2.488 ± 0.059
3.254AlaSer: 3.254 ± 0.062
3.454AlaThr: 3.454 ± 0.071
4.52AlaVal: 4.52 ± 0.079
0.541AlaTrp: 0.541 ± 0.028
2.201AlaTyr: 2.201 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.266CysAla: 0.266 ± 0.019
0.058CysCys: 0.058 ± 0.01
0.245CysAsp: 0.245 ± 0.018
0.304CysGlu: 0.304 ± 0.019
0.172CysPhe: 0.172 ± 0.013
0.496CysGly: 0.496 ± 0.029
0.168CysHis: 0.168 ± 0.017
0.325CysIle: 0.325 ± 0.02
0.333CysLys: 0.333 ± 0.023
0.414CysLeu: 0.414 ± 0.025
0.102CysMet: 0.102 ± 0.013
0.225CysAsn: 0.225 ± 0.019
0.373CysPro: 0.373 ± 0.024
0.202CysGln: 0.202 ± 0.018
0.208CysArg: 0.208 ± 0.015
0.402CysSer: 0.402 ± 0.023
0.312CysThr: 0.312 ± 0.02
0.284CysVal: 0.284 ± 0.021
0.044CysTrp: 0.044 ± 0.008
0.192CysTyr: 0.192 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
2.883AspAla: 2.883 ± 0.06
0.226AspCys: 0.226 ± 0.019
2.287AspAsp: 2.287 ± 0.067
3.979AspGlu: 3.979 ± 0.071
2.378AspPhe: 2.378 ± 0.056
3.159AspGly: 3.159 ± 0.076
1.268AspHis: 1.268 ± 0.039
4.092AspIle: 4.092 ± 0.077
3.065AspLys: 3.065 ± 0.074
5.516AspLeu: 5.516 ± 0.093
1.16AspMet: 1.16 ± 0.041
1.434AspAsn: 1.434 ± 0.052
2.167AspPro: 2.167 ± 0.056
2.965AspGln: 2.965 ± 0.078
2.233AspArg: 2.233 ± 0.051
2.311AspSer: 2.311 ± 0.06
2.283AspThr: 2.283 ± 0.058
3.495AspVal: 3.495 ± 0.072
0.624AspTrp: 0.624 ± 0.032
2.075AspTyr: 2.075 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
4.747GluAla: 4.747 ± 0.098
0.251GluCys: 0.251 ± 0.017
3.46GluAsp: 3.46 ± 0.078
6.792GluGlu: 6.792 ± 0.118
2.418GluPhe: 2.418 ± 0.061
4.148GluGly: 4.148 ± 0.073
1.512GluHis: 1.512 ± 0.047
6.818GluIle: 6.818 ± 0.097
6.916GluLys: 6.916 ± 0.115
6.919GluLeu: 6.919 ± 0.108
2.455GluMet: 2.455 ± 0.051
3.521GluAsn: 3.521 ± 0.082
1.91GluPro: 1.91 ± 0.051
3.759GluGln: 3.759 ± 0.082
3.338GluArg: 3.338 ± 0.081
3.173GluSer: 3.173 ± 0.069
3.336GluThr: 3.336 ± 0.073
5.357GluVal: 5.357 ± 0.092
0.809GluTrp: 0.809 ± 0.036
2.337GluTyr: 2.337 ± 0.053
0.0GluXaa: 0.0 ± 0.0
Phe
2.841PheAla: 2.841 ± 0.069
0.259PheCys: 0.259 ± 0.018
2.267PheAsp: 2.267 ± 0.059
2.607PheGlu: 2.607 ± 0.056
2.193PhePhe: 2.193 ± 0.072
3.182PheGly: 3.182 ± 0.06
0.967PheHis: 0.967 ± 0.036
3.89PheIle: 3.89 ± 0.084
2.536PheLys: 2.536 ± 0.062
4.545PheLeu: 4.545 ± 0.097
1.071PheMet: 1.071 ± 0.04
1.902PheAsn: 1.902 ± 0.048
1.628PhePro: 1.628 ± 0.048
1.714PheGln: 1.714 ± 0.044
1.536PheArg: 1.536 ± 0.044
2.952PheSer: 2.952 ± 0.065
2.443PheThr: 2.443 ± 0.062
2.954PheVal: 2.954 ± 0.064
0.47PheTrp: 0.47 ± 0.024
1.689PheTyr: 1.689 ± 0.052
0.0PheXaa: 0.0 ± 0.0
Gly
4.448GlyAla: 4.448 ± 0.086
0.541GlyCys: 0.541 ± 0.024
3.036GlyAsp: 3.036 ± 0.075
4.329GlyGlu: 4.329 ± 0.08
3.308GlyPhe: 3.308 ± 0.074
4.551GlyGly: 4.551 ± 0.098
1.419GlyHis: 1.419 ± 0.047
6.846GlyIle: 6.846 ± 0.103
5.296GlyLys: 5.296 ± 0.098
6.8GlyLeu: 6.8 ± 0.11
2.108GlyMet: 2.108 ± 0.056
2.704GlyAsn: 2.704 ± 0.059
1.655GlyPro: 1.655 ± 0.043
2.391GlyGln: 2.391 ± 0.059
2.618GlyArg: 2.618 ± 0.066
3.603GlySer: 3.603 ± 0.078
3.939GlyThr: 3.939 ± 0.077
5.102GlyVal: 5.102 ± 0.087
0.759GlyTrp: 0.759 ± 0.036
2.849GlyTyr: 2.849 ± 0.064
0.0GlyXaa: 0.0 ± 0.0
His
1.179HisAla: 1.179 ± 0.038
0.164HisCys: 0.164 ± 0.016
0.966HisAsp: 0.966 ± 0.037
1.235HisGlu: 1.235 ± 0.038
1.07HisPhe: 1.07 ± 0.046
1.399HisGly: 1.399 ± 0.046
0.78HisHis: 0.78 ± 0.042
1.656HisIle: 1.656 ± 0.047
1.021HisLys: 1.021 ± 0.042
2.424HisLeu: 2.424 ± 0.068
0.505HisMet: 0.505 ± 0.027
0.684HisAsn: 0.684 ± 0.033
1.205HisPro: 1.205 ± 0.04
1.11HisGln: 1.11 ± 0.048
0.919HisArg: 0.919 ± 0.036
1.258HisSer: 1.258 ± 0.041
0.98HisThr: 0.98 ± 0.035
1.387HisVal: 1.387 ± 0.042
0.262HisTrp: 0.262 ± 0.02
0.878HisTyr: 0.878 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
6.473IleAla: 6.473 ± 0.098
0.43IleCys: 0.43 ± 0.023
5.042IleAsp: 5.042 ± 0.088
6.833IleGlu: 6.833 ± 0.111
3.624IlePhe: 3.624 ± 0.084
6.732IleGly: 6.732 ± 0.116
1.909IleHis: 1.909 ± 0.045
6.636IleIle: 6.636 ± 0.116
5.789IleLys: 5.789 ± 0.082
8.572IleLeu: 8.572 ± 0.129
1.88IleMet: 1.88 ± 0.048
3.723IleAsn: 3.723 ± 0.078
3.984IlePro: 3.984 ± 0.077
3.731IleGln: 3.731 ± 0.072
3.557IleArg: 3.557 ± 0.074
5.316IleSer: 5.316 ± 0.088
4.608IleThr: 4.608 ± 0.094
5.775IleVal: 5.775 ± 0.088
0.739IleTrp: 0.739 ± 0.034
2.836IleTyr: 2.836 ± 0.065
0.0IleXaa: 0.0 ± 0.0
Lys
4.655LysAla: 4.655 ± 0.081
0.276LysCys: 0.276 ± 0.022
3.931LysAsp: 3.931 ± 0.081
7.543LysGlu: 7.543 ± 0.107
1.903LysPhe: 1.903 ± 0.053
4.861LysGly: 4.861 ± 0.094
1.29LysHis: 1.29 ± 0.045
5.997LysIle: 5.997 ± 0.095
6.172LysLys: 6.172 ± 0.101
5.834LysLeu: 5.834 ± 0.089
2.316LysMet: 2.316 ± 0.057
3.637LysAsn: 3.637 ± 0.067
2.377LysPro: 2.377 ± 0.061
3.431LysGln: 3.431 ± 0.075
3.325LysArg: 3.325 ± 0.068
3.221LysSer: 3.221 ± 0.067
3.751LysThr: 3.751 ± 0.065
5.389LysVal: 5.389 ± 0.075
0.872LysTrp: 0.872 ± 0.035
2.443LysTyr: 2.443 ± 0.065
0.0LysXaa: 0.0 ± 0.0
Leu
6.852LeuAla: 6.852 ± 0.106
0.377LeuCys: 0.377 ± 0.024
4.981LeuAsp: 4.981 ± 0.09
6.721LeuGlu: 6.721 ± 0.124
4.829LeuPhe: 4.829 ± 0.09
6.899LeuGly: 6.899 ± 0.102
1.966LeuHis: 1.966 ± 0.048
7.951LeuIle: 7.951 ± 0.127
7.377LeuLys: 7.377 ± 0.098
9.949LeuLeu: 9.949 ± 0.174
2.491LeuMet: 2.491 ± 0.058
4.68LeuAsn: 4.68 ± 0.079
3.894LeuPro: 3.894 ± 0.076
3.812LeuGln: 3.812 ± 0.087
3.717LeuArg: 3.717 ± 0.071
6.668LeuSer: 6.668 ± 0.103
5.621LeuThr: 5.621 ± 0.079
6.469LeuVal: 6.469 ± 0.107
0.786LeuTrp: 0.786 ± 0.04
3.09LeuTyr: 3.09 ± 0.075
0.0LeuXaa: 0.0 ± 0.0
Met
2.156MetAla: 2.156 ± 0.064
0.101MetCys: 0.101 ± 0.012
1.353MetAsp: 1.353 ± 0.044
1.96MetGlu: 1.96 ± 0.051
0.946MetPhe: 0.946 ± 0.038
1.86MetGly: 1.86 ± 0.052
0.344MetHis: 0.344 ± 0.02
2.85MetIle: 2.85 ± 0.062
2.465MetLys: 2.465 ± 0.055
2.239MetLeu: 2.239 ± 0.054
0.989MetMet: 0.989 ± 0.037
1.484MetAsn: 1.484 ± 0.043
0.905MetPro: 0.905 ± 0.04
0.854MetGln: 0.854 ± 0.034
0.985MetArg: 0.985 ± 0.039
1.526MetSer: 1.526 ± 0.045
1.509MetThr: 1.509 ± 0.045
2.14MetVal: 2.14 ± 0.052
0.161MetTrp: 0.161 ± 0.015
0.698MetTyr: 0.698 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
2.476AsnAla: 2.476 ± 0.056
0.213AsnCys: 0.213 ± 0.018
2.188AsnAsp: 2.188 ± 0.052
3.427AsnGlu: 3.427 ± 0.071
1.637AsnPhe: 1.637 ± 0.048
3.21AsnGly: 3.21 ± 0.068
1.247AsnHis: 1.247 ± 0.043
3.551AsnIle: 3.551 ± 0.068
3.038AsnLys: 3.038 ± 0.075
4.124AsnLeu: 4.124 ± 0.084
1.18AsnMet: 1.18 ± 0.036
1.772AsnAsn: 1.772 ± 0.061
2.223AsnPro: 2.223 ± 0.057
2.741AsnGln: 2.741 ± 0.067
2.108AsnArg: 2.108 ± 0.055
2.1AsnSer: 2.1 ± 0.062
2.071AsnThr: 2.071 ± 0.055
2.811AsnVal: 2.811 ± 0.057
0.491AsnTrp: 0.491 ± 0.028
1.569AsnTyr: 1.569 ± 0.052
0.0AsnXaa: 0.0 ± 0.0
Pro
1.988ProAla: 1.988 ± 0.06
0.176ProCys: 0.176 ± 0.014
1.973ProAsp: 1.973 ± 0.052
2.943ProGlu: 2.943 ± 0.064
1.96ProPhe: 1.96 ± 0.045
2.296ProGly: 2.296 ± 0.062
0.754ProHis: 0.754 ± 0.033
3.551ProIle: 3.551 ± 0.069
2.443ProLys: 2.443 ± 0.056
3.536ProLeu: 3.536 ± 0.075
0.862ProMet: 0.862 ± 0.037
1.91ProAsn: 1.91 ± 0.058
0.984ProPro: 0.984 ± 0.039
1.144ProGln: 1.144 ± 0.042
1.13ProArg: 1.13 ± 0.034
2.12ProSer: 2.12 ± 0.054
2.135ProThr: 2.135 ± 0.06
2.787ProVal: 2.787 ± 0.066
0.425ProTrp: 0.425 ± 0.029
1.518ProTyr: 1.518 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
2.778GlnAla: 2.778 ± 0.07
0.159GlnCys: 0.159 ± 0.015
1.722GlnAsp: 1.722 ± 0.053
3.25GlnGlu: 3.25 ± 0.064
1.811GlnPhe: 1.811 ± 0.046
2.448GlnGly: 2.448 ± 0.059
0.79GlnHis: 0.79 ± 0.035
3.926GlnIle: 3.926 ± 0.083
3.133GlnLys: 3.133 ± 0.068
4.775GlnLeu: 4.775 ± 0.094
1.263GlnMet: 1.263 ± 0.04
1.763GlnAsn: 1.763 ± 0.051
1.373GlnPro: 1.373 ± 0.048
2.063GlnGln: 2.063 ± 0.067
1.566GlnArg: 1.566 ± 0.045
2.208GlnSer: 2.208 ± 0.052
2.026GlnThr: 2.026 ± 0.049
2.999GlnVal: 2.999 ± 0.063
0.504GlnTrp: 0.504 ± 0.025
1.656GlnTyr: 1.656 ± 0.05
0.0GlnXaa: 0.0 ± 0.0
Arg
2.172ArgAla: 2.172 ± 0.061
0.247ArgCys: 0.247 ± 0.017
1.979ArgAsp: 1.979 ± 0.055
3.1ArgGlu: 3.1 ± 0.069
1.93ArgPhe: 1.93 ± 0.049
2.422ArgGly: 2.422 ± 0.056
0.763ArgHis: 0.763 ± 0.029
3.643ArgIle: 3.643 ± 0.074
3.292ArgLys: 3.292 ± 0.068
4.088ArgLeu: 4.088 ± 0.082
1.247ArgMet: 1.247 ± 0.043
1.857ArgAsn: 1.857 ± 0.048
1.337ArgPro: 1.337 ± 0.041
1.553ArgGln: 1.553 ± 0.046
1.709ArgArg: 1.709 ± 0.054
2.104ArgSer: 2.104 ± 0.057
1.934ArgThr: 1.934 ± 0.044
2.637ArgVal: 2.637 ± 0.057
0.436ArgTrp: 0.436 ± 0.022
1.577ArgTyr: 1.577 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
3.12SerAla: 3.12 ± 0.07
0.337SerCys: 0.337 ± 0.023
2.52SerAsp: 2.52 ± 0.055
3.485SerGlu: 3.485 ± 0.063
2.919SerPhe: 2.919 ± 0.064
3.938SerGly: 3.938 ± 0.074
1.16SerHis: 1.16 ± 0.039
5.233SerIle: 5.233 ± 0.092
3.84SerLys: 3.84 ± 0.07
5.927SerLeu: 5.927 ± 0.099
1.612SerMet: 1.612 ± 0.05
2.361SerAsn: 2.361 ± 0.057
2.007SerPro: 2.007 ± 0.051
1.991SerGln: 1.991 ± 0.057
2.157SerArg: 2.157 ± 0.056
3.379SerSer: 3.379 ± 0.077
3.044SerThr: 3.044 ± 0.053
3.522SerVal: 3.522 ± 0.065
0.595SerTrp: 0.595 ± 0.031
2.055SerTyr: 2.055 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
3.431ThrAla: 3.431 ± 0.07
0.31ThrCys: 0.31 ± 0.02
2.472ThrAsp: 2.472 ± 0.059
3.329ThrGlu: 3.329 ± 0.072
2.493ThrPhe: 2.493 ± 0.064
4.098ThrGly: 4.098 ± 0.072
0.984ThrHis: 0.984 ± 0.033
5.042ThrIle: 5.042 ± 0.083
3.514ThrLys: 3.514 ± 0.079
5.27ThrLeu: 5.27 ± 0.089
1.331ThrMet: 1.331 ± 0.039
2.496ThrAsn: 2.496 ± 0.064
2.223ThrPro: 2.223 ± 0.056
1.614ThrGln: 1.614 ± 0.053
1.854ThrArg: 1.854 ± 0.048
3.03ThrSer: 3.03 ± 0.059
2.907ThrThr: 2.907 ± 0.065
3.657ThrVal: 3.657 ± 0.069
0.504ThrTrp: 0.504 ± 0.025
1.905ThrTyr: 1.905 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
4.727ValAla: 4.727 ± 0.095
0.386ValCys: 0.386 ± 0.025
3.754ValAsp: 3.754 ± 0.072
4.939ValGlu: 4.939 ± 0.092
2.877ValPhe: 2.877 ± 0.058
4.796ValGly: 4.796 ± 0.08
1.377ValHis: 1.377 ± 0.049
6.375ValIle: 6.375 ± 0.092
4.891ValLys: 4.891 ± 0.072
6.407ValLeu: 6.407 ± 0.109
1.841ValMet: 1.841 ± 0.052
3.286ValAsn: 3.286 ± 0.07
2.694ValPro: 2.694 ± 0.06
2.489ValGln: 2.489 ± 0.054
2.529ValArg: 2.529 ± 0.058
4.07ValSer: 4.07 ± 0.078
3.838ValThr: 3.838 ± 0.072
5.12ValVal: 5.12 ± 0.089
0.634ValTrp: 0.634 ± 0.03
2.235ValTyr: 2.235 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.552TrpAla: 0.552 ± 0.028
0.049TrpCys: 0.049 ± 0.008
0.529TrpAsp: 0.529 ± 0.025
0.624TrpGlu: 0.624 ± 0.032
0.583TrpPhe: 0.583 ± 0.032
0.606TrpGly: 0.606 ± 0.03
0.184TrpHis: 0.184 ± 0.017
0.926TrpIle: 0.926 ± 0.042
0.753TrpLys: 0.753 ± 0.032
1.182TrpLeu: 1.182 ± 0.046
0.34TrpMet: 0.34 ± 0.023
0.581TrpAsn: 0.581 ± 0.031
0.304TrpPro: 0.304 ± 0.022
0.463TrpGln: 0.463 ± 0.025
0.369TrpArg: 0.369 ± 0.025
0.47TrpSer: 0.47 ± 0.027
0.444TrpThr: 0.444 ± 0.023
0.7TrpVal: 0.7 ± 0.032
0.153TrpTrp: 0.153 ± 0.015
0.345TrpTyr: 0.345 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.95TyrAla: 1.95 ± 0.053
0.208TyrCys: 0.208 ± 0.017
1.915TyrAsp: 1.915 ± 0.061
2.264TyrGlu: 2.264 ± 0.059
1.717TyrPhe: 1.717 ± 0.045
2.547TyrGly: 2.547 ± 0.061
1.017TyrHis: 1.017 ± 0.035
2.604TyrIle: 2.604 ± 0.059
2.033TyrLys: 2.033 ± 0.056
3.926TyrLeu: 3.926 ± 0.078
0.82TyrMet: 0.82 ± 0.029
1.366TyrAsn: 1.366 ± 0.049
1.607TyrPro: 1.607 ± 0.051
2.115TyrGln: 2.115 ± 0.061
1.682TyrArg: 1.682 ± 0.044
2.037TyrSer: 2.037 ± 0.053
1.828TyrThr: 1.828 ± 0.053
2.164TyrVal: 2.164 ± 0.049
0.348TyrTrp: 0.348 ± 0.024
1.458TyrTyr: 1.458 ± 0.047
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2599 proteins (756038 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski