Criibacterium bergeronii
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2051 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A371IM61|A0A371IM61_9FIRM AraC family transcriptional regulator OS=Criibacterium bergeronii OX=1871336 GN=BBG48_004015 PE=4 SV=1
MM1 pKa = 7.18 STTKK5 pKa = 10.09 EE6 pKa = 3.72 IEE8 pKa = 3.99 IIGCINVPEE17 pKa = 4.37 EE18 pKa = 3.95 VSSDD22 pKa = 3.49 EE23 pKa = 4.78 VIDD26 pKa = 3.68 TFIEE30 pKa = 4.43 YY31 pKa = 10.4 VEE33 pKa = 3.99 SHH35 pKa = 5.32 GWFFGGGFRR44 pKa = 11.84 TIQDD48 pKa = 3.56 GYY50 pKa = 10.46 YY51 pKa = 10.0 INADD55 pKa = 3.41 GTKK58 pKa = 9.98 AEE60 pKa = 4.61 PVLGDD65 pKa = 3.5 YY66 pKa = 10.85
Molecular weight: 7.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.8
IPC2_protein 3.973
IPC_protein 3.834
Toseland 3.668
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.681
Grimsley 3.592
Solomon 3.783
Lehninger 3.732
Nozaki 3.948
DTASelect 4.062
Thurlkill 3.719
EMBOSS 3.732
Sillero 3.961
Patrickios 0.693
IPC_peptide 3.783
IPC2_peptide 3.935
IPC2.peptide.svr19 3.856
Protein with the highest isoelectric point:
>tr|A0A371IM76|A0A371IM76_9FIRM Uncharacterized protein OS=Criibacterium bergeronii OX=1871336 GN=BBG48_004440 PE=4 SV=1
MM1 pKa = 7.54 NKK3 pKa = 8.34 GTYY6 pKa = 7.76 QPKK9 pKa = 9.42 KK10 pKa = 7.55 RR11 pKa = 11.84 QRR13 pKa = 11.84 SKK15 pKa = 9.64 VHH17 pKa = 6.24 GFRR20 pKa = 11.84 QRR22 pKa = 11.84 MRR24 pKa = 11.84 TVGGRR29 pKa = 11.84 NVLRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.14 GRR40 pKa = 11.84 KK41 pKa = 8.79 KK42 pKa = 10.63 LSAA45 pKa = 3.95
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2051
0
2051
621987
22
2041
303.3
34.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.278 ± 0.062
0.941 ± 0.02
6.17 ± 0.046
6.859 ± 0.058
4.445 ± 0.045
6.025 ± 0.059
1.322 ± 0.02
9.056 ± 0.063
9.187 ± 0.058
8.911 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.764 ± 0.03
5.825 ± 0.045
2.819 ± 0.036
3.197 ± 0.035
3.252 ± 0.039
6.434 ± 0.046
5.367 ± 0.047
6.386 ± 0.042
0.557 ± 0.015
4.207 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here