Beihai narna-like virus 26
Average proteome isoelectric point is 9.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KHU6|A0A1L3KHU6_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 26 OX=1922454 PE=4 SV=1
MM1 pKa = 7.19 IQAKK5 pKa = 8.01 QMCARR10 pKa = 11.84 LMSLGIPKK18 pKa = 9.85 HH19 pKa = 5.74 VSSDD23 pKa = 3.34 IINLIEE29 pKa = 3.8 KK30 pKa = 9.5 WRR32 pKa = 11.84 DD33 pKa = 3.0 KK34 pKa = 11.24 SGNEE38 pKa = 3.46 WTVNRR43 pKa = 11.84 LKK45 pKa = 10.43 EE46 pKa = 4.07 YY47 pKa = 10.31 KK48 pKa = 10.03 QLFIAKK54 pKa = 9.54 LANTTVNHH62 pKa = 4.82 TWIARR67 pKa = 11.84 NKK69 pKa = 10.01 DD70 pKa = 3.87 GYY72 pKa = 9.95 PKK74 pKa = 10.94 GPFSYY79 pKa = 10.18 IFRR82 pKa = 11.84 RR83 pKa = 11.84 LQNKK87 pKa = 10.06 GIDD90 pKa = 3.56 KK91 pKa = 10.61 ALSALQSYY99 pKa = 8.21 TYY101 pKa = 10.21 FVSNEE106 pKa = 3.66 PTKK109 pKa = 10.97 AQTEE113 pKa = 4.41 KK114 pKa = 10.82 FMSALLSKK122 pKa = 9.88 EE123 pKa = 3.68 SRR125 pKa = 11.84 GIEE128 pKa = 3.74 SRR130 pKa = 11.84 FKK132 pKa = 10.76 HH133 pKa = 5.3 FHH135 pKa = 6.0 PLIKK139 pKa = 9.98 EE140 pKa = 3.88 RR141 pKa = 11.84 RR142 pKa = 11.84 APEE145 pKa = 3.99 APPTIFEE152 pKa = 4.54 YY153 pKa = 10.67 CVSHH157 pKa = 5.49 TRR159 pKa = 11.84 MPGLTKK165 pKa = 10.55 SIPEE169 pKa = 3.66 MGNYY173 pKa = 9.2 VEE175 pKa = 4.08 AAIRR179 pKa = 11.84 AGSVEE184 pKa = 4.61 CIRR187 pKa = 11.84 SCMVSNPEE195 pKa = 3.61 VFEE198 pKa = 4.2 NVVPLDD204 pKa = 3.54 TVLDD208 pKa = 3.86 VEE210 pKa = 4.8 PYY212 pKa = 10.51 RR213 pKa = 11.84 MSGHH217 pKa = 7.24 PYY219 pKa = 7.68 ATQSFGYY226 pKa = 10.08 LSGIQEE232 pKa = 4.2 PGYY235 pKa = 10.17 KK236 pKa = 9.95 LRR238 pKa = 11.84 VVANPDD244 pKa = 3.06 RR245 pKa = 11.84 VVQAGLEE252 pKa = 4.01 PLKK255 pKa = 11.18 NEE257 pKa = 4.31 LRR259 pKa = 11.84 RR260 pKa = 11.84 SLEE263 pKa = 4.59 KK264 pKa = 10.38 IPEE267 pKa = 4.47 DD268 pKa = 3.45 CTFDD272 pKa = 3.63 QEE274 pKa = 4.36 KK275 pKa = 10.84 ALIPIQKK282 pKa = 8.54 WLNSGRR288 pKa = 11.84 TVYY291 pKa = 10.86 SVDD294 pKa = 3.92 LSDD297 pKa = 4.31 ATTLFPWKK305 pKa = 9.32 VTEE308 pKa = 4.11 DD309 pKa = 3.69 LLRR312 pKa = 11.84 KK313 pKa = 9.87 RR314 pKa = 11.84 FPSYY318 pKa = 10.11 QSHH321 pKa = 6.7 IDD323 pKa = 3.59 VMDD326 pKa = 3.94 SASRR330 pKa = 11.84 GPWTLKK336 pKa = 9.75 FGKK339 pKa = 9.72 RR340 pKa = 11.84 RR341 pKa = 11.84 NKK343 pKa = 10.41 DD344 pKa = 3.07 CTVQFTRR351 pKa = 11.84 GQPLGLGPSFFAFSLTHH368 pKa = 6.67 HH369 pKa = 6.49 NLLYY373 pKa = 10.51 GICKK377 pKa = 10.48 KK378 pKa = 10.52 LGKK381 pKa = 8.25 STKK384 pKa = 9.7 TNDD387 pKa = 2.54 IYY389 pKa = 10.98 RR390 pKa = 11.84 ILGDD394 pKa = 3.84 DD395 pKa = 4.2 VVISDD400 pKa = 3.96 HH401 pKa = 6.85 KK402 pKa = 9.67 VAKK405 pKa = 10.45 NYY407 pKa = 8.32 MRR409 pKa = 11.84 TLRR412 pKa = 11.84 NLGCKK417 pKa = 9.65 ISEE420 pKa = 4.12 NKK422 pKa = 8.99 TLISSYY428 pKa = 9.89 AAEE431 pKa = 3.92 FAGYY435 pKa = 10.17 VVTRR439 pKa = 11.84 QNVGKK444 pKa = 10.08 GMKK447 pKa = 8.79 WRR449 pKa = 11.84 EE450 pKa = 3.94 LSNHH454 pKa = 5.85 SFLEE458 pKa = 4.38 TVKK461 pKa = 10.87 RR462 pKa = 11.84 LGKK465 pKa = 10.26 RR466 pKa = 11.84 SLPLLTSNQRR476 pKa = 11.84 ALVKK480 pKa = 10.18 FIGPVPRR487 pKa = 11.84 SLGGLGWGSGRR498 pKa = 11.84 KK499 pKa = 9.32 LDD501 pKa = 5.42 DD502 pKa = 4.16 FFSSNIGKK510 pKa = 10.32 ASIDD514 pKa = 3.55 AYY516 pKa = 10.24 IDD518 pKa = 3.44 IVDD521 pKa = 4.17 KK522 pKa = 11.0 KK523 pKa = 10.36 LARR526 pKa = 11.84 AFPCEE531 pKa = 3.9 SLDD534 pKa = 3.39 YY535 pKa = 10.3 HH536 pKa = 6.97 ARR538 pKa = 11.84 RR539 pKa = 11.84 LSSGVRR545 pKa = 11.84 GYY547 pKa = 9.81 KK548 pKa = 9.71 HH549 pKa = 6.5 CSEE552 pKa = 4.31 NLFHH556 pKa = 7.87 RR557 pKa = 11.84 EE558 pKa = 3.65 FRR560 pKa = 11.84 EE561 pKa = 4.21 KK562 pKa = 10.95 DD563 pKa = 3.22 PSDD566 pKa = 4.26 NDD568 pKa = 2.89 WMAWKK573 pKa = 9.88 QAINLEE579 pKa = 4.32 YY580 pKa = 10.43 QDD582 pKa = 3.9 FLPRR586 pKa = 11.84 EE587 pKa = 4.22 TRR589 pKa = 11.84 EE590 pKa = 4.0 KK591 pKa = 10.8 LDD593 pKa = 3.61 PDD595 pKa = 3.51 VPLRR599 pKa = 11.84 VISKK603 pKa = 7.32 EE604 pKa = 4.1 TPPGFFRR611 pKa = 11.84 LYY613 pKa = 10.23 RR614 pKa = 11.84 QTGDD618 pKa = 3.4 PRR620 pKa = 11.84 PSVTTSAFIIYY631 pKa = 9.34 KK632 pKa = 10.14 AHH634 pKa = 6.37 LVDD637 pKa = 4.13 GGVRR641 pKa = 11.84 NPAMEE646 pKa = 4.76 RR647 pKa = 11.84 FFQKK651 pKa = 9.51 TFNSSFDD658 pKa = 3.72 ALDD661 pKa = 3.39 LVVNNTFEE669 pKa = 4.51 TYY671 pKa = 10.1 RR672 pKa = 11.84 SYY674 pKa = 11.07 HH675 pKa = 5.41 QIRR678 pKa = 11.84 KK679 pKa = 9.63 RR680 pKa = 11.84 SVPKK684 pKa = 10.43 PNVKK688 pKa = 9.6 TISSKK693 pKa = 10.64 VKK695 pKa = 10.03 SPKK698 pKa = 9.69 KK699 pKa = 9.63 RR700 pKa = 11.84 ALNRR704 pKa = 11.84 NRR706 pKa = 11.84 GPRR709 pKa = 11.84 LL710 pKa = 3.23
Molecular weight: 81.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.105
IPC2_protein 9.18
IPC_protein 9.121
Toseland 9.911
ProMoST 9.589
Dawson 10.116
Bjellqvist 9.794
Wikipedia 10.277
Rodwell 10.555
Grimsley 10.189
Solomon 10.131
Lehninger 10.101
Nozaki 9.955
DTASelect 9.765
Thurlkill 9.97
EMBOSS 10.321
Sillero 10.043
Patrickios 9.94
IPC_peptide 10.145
IPC2_peptide 8.404
IPC2.peptide.svr19 8.125
Protein with the highest isoelectric point:
>tr|A0A1L3KHU6|A0A1L3KHU6_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 26 OX=1922454 PE=4 SV=1
MM1 pKa = 7.19 IQAKK5 pKa = 8.01 QMCARR10 pKa = 11.84 LMSLGIPKK18 pKa = 9.85 HH19 pKa = 5.74 VSSDD23 pKa = 3.34 IINLIEE29 pKa = 3.8 KK30 pKa = 9.5 WRR32 pKa = 11.84 DD33 pKa = 3.0 KK34 pKa = 11.24 SGNEE38 pKa = 3.46 WTVNRR43 pKa = 11.84 LKK45 pKa = 10.43 EE46 pKa = 4.07 YY47 pKa = 10.31 KK48 pKa = 10.03 QLFIAKK54 pKa = 9.54 LANTTVNHH62 pKa = 4.82 TWIARR67 pKa = 11.84 NKK69 pKa = 10.01 DD70 pKa = 3.87 GYY72 pKa = 9.95 PKK74 pKa = 10.94 GPFSYY79 pKa = 10.18 IFRR82 pKa = 11.84 RR83 pKa = 11.84 LQNKK87 pKa = 10.06 GIDD90 pKa = 3.56 KK91 pKa = 10.61 ALSALQSYY99 pKa = 8.21 TYY101 pKa = 10.21 FVSNEE106 pKa = 3.66 PTKK109 pKa = 10.97 AQTEE113 pKa = 4.41 KK114 pKa = 10.82 FMSALLSKK122 pKa = 9.88 EE123 pKa = 3.68 SRR125 pKa = 11.84 GIEE128 pKa = 3.74 SRR130 pKa = 11.84 FKK132 pKa = 10.76 HH133 pKa = 5.3 FHH135 pKa = 6.0 PLIKK139 pKa = 9.98 EE140 pKa = 3.88 RR141 pKa = 11.84 RR142 pKa = 11.84 APEE145 pKa = 3.99 APPTIFEE152 pKa = 4.54 YY153 pKa = 10.67 CVSHH157 pKa = 5.49 TRR159 pKa = 11.84 MPGLTKK165 pKa = 10.55 SIPEE169 pKa = 3.66 MGNYY173 pKa = 9.2 VEE175 pKa = 4.08 AAIRR179 pKa = 11.84 AGSVEE184 pKa = 4.61 CIRR187 pKa = 11.84 SCMVSNPEE195 pKa = 3.61 VFEE198 pKa = 4.2 NVVPLDD204 pKa = 3.54 TVLDD208 pKa = 3.86 VEE210 pKa = 4.8 PYY212 pKa = 10.51 RR213 pKa = 11.84 MSGHH217 pKa = 7.24 PYY219 pKa = 7.68 ATQSFGYY226 pKa = 10.08 LSGIQEE232 pKa = 4.2 PGYY235 pKa = 10.17 KK236 pKa = 9.95 LRR238 pKa = 11.84 VVANPDD244 pKa = 3.06 RR245 pKa = 11.84 VVQAGLEE252 pKa = 4.01 PLKK255 pKa = 11.18 NEE257 pKa = 4.31 LRR259 pKa = 11.84 RR260 pKa = 11.84 SLEE263 pKa = 4.59 KK264 pKa = 10.38 IPEE267 pKa = 4.47 DD268 pKa = 3.45 CTFDD272 pKa = 3.63 QEE274 pKa = 4.36 KK275 pKa = 10.84 ALIPIQKK282 pKa = 8.54 WLNSGRR288 pKa = 11.84 TVYY291 pKa = 10.86 SVDD294 pKa = 3.92 LSDD297 pKa = 4.31 ATTLFPWKK305 pKa = 9.32 VTEE308 pKa = 4.11 DD309 pKa = 3.69 LLRR312 pKa = 11.84 KK313 pKa = 9.87 RR314 pKa = 11.84 FPSYY318 pKa = 10.11 QSHH321 pKa = 6.7 IDD323 pKa = 3.59 VMDD326 pKa = 3.94 SASRR330 pKa = 11.84 GPWTLKK336 pKa = 9.75 FGKK339 pKa = 9.72 RR340 pKa = 11.84 RR341 pKa = 11.84 NKK343 pKa = 10.41 DD344 pKa = 3.07 CTVQFTRR351 pKa = 11.84 GQPLGLGPSFFAFSLTHH368 pKa = 6.67 HH369 pKa = 6.49 NLLYY373 pKa = 10.51 GICKK377 pKa = 10.48 KK378 pKa = 10.52 LGKK381 pKa = 8.25 STKK384 pKa = 9.7 TNDD387 pKa = 2.54 IYY389 pKa = 10.98 RR390 pKa = 11.84 ILGDD394 pKa = 3.84 DD395 pKa = 4.2 VVISDD400 pKa = 3.96 HH401 pKa = 6.85 KK402 pKa = 9.67 VAKK405 pKa = 10.45 NYY407 pKa = 8.32 MRR409 pKa = 11.84 TLRR412 pKa = 11.84 NLGCKK417 pKa = 9.65 ISEE420 pKa = 4.12 NKK422 pKa = 8.99 TLISSYY428 pKa = 9.89 AAEE431 pKa = 3.92 FAGYY435 pKa = 10.17 VVTRR439 pKa = 11.84 QNVGKK444 pKa = 10.08 GMKK447 pKa = 8.79 WRR449 pKa = 11.84 EE450 pKa = 3.94 LSNHH454 pKa = 5.85 SFLEE458 pKa = 4.38 TVKK461 pKa = 10.87 RR462 pKa = 11.84 LGKK465 pKa = 10.26 RR466 pKa = 11.84 SLPLLTSNQRR476 pKa = 11.84 ALVKK480 pKa = 10.18 FIGPVPRR487 pKa = 11.84 SLGGLGWGSGRR498 pKa = 11.84 KK499 pKa = 9.32 LDD501 pKa = 5.42 DD502 pKa = 4.16 FFSSNIGKK510 pKa = 10.32 ASIDD514 pKa = 3.55 AYY516 pKa = 10.24 IDD518 pKa = 3.44 IVDD521 pKa = 4.17 KK522 pKa = 11.0 KK523 pKa = 10.36 LARR526 pKa = 11.84 AFPCEE531 pKa = 3.9 SLDD534 pKa = 3.39 YY535 pKa = 10.3 HH536 pKa = 6.97 ARR538 pKa = 11.84 RR539 pKa = 11.84 LSSGVRR545 pKa = 11.84 GYY547 pKa = 9.81 KK548 pKa = 9.71 HH549 pKa = 6.5 CSEE552 pKa = 4.31 NLFHH556 pKa = 7.87 RR557 pKa = 11.84 EE558 pKa = 3.65 FRR560 pKa = 11.84 EE561 pKa = 4.21 KK562 pKa = 10.95 DD563 pKa = 3.22 PSDD566 pKa = 4.26 NDD568 pKa = 2.89 WMAWKK573 pKa = 9.88 QAINLEE579 pKa = 4.32 YY580 pKa = 10.43 QDD582 pKa = 3.9 FLPRR586 pKa = 11.84 EE587 pKa = 4.22 TRR589 pKa = 11.84 EE590 pKa = 4.0 KK591 pKa = 10.8 LDD593 pKa = 3.61 PDD595 pKa = 3.51 VPLRR599 pKa = 11.84 VISKK603 pKa = 7.32 EE604 pKa = 4.1 TPPGFFRR611 pKa = 11.84 LYY613 pKa = 10.23 RR614 pKa = 11.84 QTGDD618 pKa = 3.4 PRR620 pKa = 11.84 PSVTTSAFIIYY631 pKa = 9.34 KK632 pKa = 10.14 AHH634 pKa = 6.37 LVDD637 pKa = 4.13 GGVRR641 pKa = 11.84 NPAMEE646 pKa = 4.76 RR647 pKa = 11.84 FFQKK651 pKa = 9.51 TFNSSFDD658 pKa = 3.72 ALDD661 pKa = 3.39 LVVNNTFEE669 pKa = 4.51 TYY671 pKa = 10.1 RR672 pKa = 11.84 SYY674 pKa = 11.07 HH675 pKa = 5.41 QIRR678 pKa = 11.84 KK679 pKa = 9.63 RR680 pKa = 11.84 SVPKK684 pKa = 10.43 PNVKK688 pKa = 9.6 TISSKK693 pKa = 10.64 VKK695 pKa = 10.03 SPKK698 pKa = 9.69 KK699 pKa = 9.63 RR700 pKa = 11.84 ALNRR704 pKa = 11.84 NRR706 pKa = 11.84 GPRR709 pKa = 11.84 LL710 pKa = 3.23
Molecular weight: 81.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.105
IPC2_protein 9.18
IPC_protein 9.121
Toseland 9.911
ProMoST 9.589
Dawson 10.116
Bjellqvist 9.794
Wikipedia 10.277
Rodwell 10.555
Grimsley 10.189
Solomon 10.131
Lehninger 10.101
Nozaki 9.955
DTASelect 9.765
Thurlkill 9.97
EMBOSS 10.321
Sillero 10.043
Patrickios 9.94
IPC_peptide 10.145
IPC2_peptide 8.404
IPC2.peptide.svr19 8.125
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
710
710
710
710.0
81.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.352 ± 0.0
1.408 ± 0.0
4.93 ± 0.0
5.211 ± 0.0
4.93 ± 0.0
5.915 ± 0.0
2.254 ± 0.0
5.211 ± 0.0
8.169 ± 0.0
8.732 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.831 ± 0.0
4.789 ± 0.0
5.775 ± 0.0
2.958 ± 0.0
7.887 ± 0.0
8.31 ± 0.0
5.211 ± 0.0
6.056 ± 0.0
1.408 ± 0.0
3.662 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here