Microviridae Fen685_11

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2UMG1|A0A0G2UMG1_9VIRU Major capsid protein VP1 OS=Microviridae Fen685_11 OX=1655657 PE=3 SV=1
MM1 pKa = 7.36QPMLAPVLHH10 pKa = 6.67RR11 pKa = 11.84LSIRR15 pKa = 11.84SEE17 pKa = 3.56WFFIPNRR24 pKa = 11.84ILWPNWEE31 pKa = 4.52SFISPPVEE39 pKa = 4.43GVDD42 pKa = 4.08GPAAPFLSNFTVVSNSLGDD61 pKa = 3.89YY62 pKa = 10.69LGLPLGSWNNGDD74 pKa = 4.46GGDD77 pKa = 3.73PSQIVNALPFSAYY90 pKa = 9.24QRR92 pKa = 11.84VWWDD96 pKa = 2.86WYY98 pKa = 10.54RR99 pKa = 11.84DD100 pKa = 3.34EE101 pKa = 6.28DD102 pKa = 4.08FQPLQWQYY110 pKa = 11.87AGQLVDD116 pKa = 4.39GDD118 pKa = 3.72NSEE121 pKa = 4.16QEE123 pKa = 4.02EE124 pKa = 4.61LFPIRR129 pKa = 11.84IRR131 pKa = 11.84NNDD134 pKa = 2.9RR135 pKa = 11.84DD136 pKa = 4.19YY137 pKa = 10.75FTSAKK142 pKa = 9.64PWPQKK147 pKa = 10.39GDD149 pKa = 3.36AVVIPAFVGDD159 pKa = 4.45LPVSANLEE167 pKa = 4.55TQWHH171 pKa = 5.33VNRR174 pKa = 11.84TDD176 pKa = 3.24HH177 pKa = 6.66TGLSATALVSTGTSDD192 pKa = 3.16TDD194 pKa = 4.71GIGLDD199 pKa = 3.69GGGTGVALDD208 pKa = 3.81PRR210 pKa = 11.84GALSISSDD218 pKa = 4.22DD219 pKa = 3.85MLAQAGTIEE228 pKa = 4.23QLRR231 pKa = 11.84MANALQRR238 pKa = 11.84FLEE241 pKa = 4.42ADD243 pKa = 3.0ARR245 pKa = 11.84GGTRR249 pKa = 11.84YY250 pKa = 9.86VEE252 pKa = 3.94LLWRR256 pKa = 11.84HH257 pKa = 5.84FKK259 pKa = 10.77AYY261 pKa = 10.6LPDD264 pKa = 5.07KK265 pKa = 10.05IDD267 pKa = 3.41RR268 pKa = 11.84SEE270 pKa = 4.34YY271 pKa = 10.15IGNTVQPISISEE283 pKa = 4.1VLNTTGTTDD292 pKa = 3.23APQGTMTGHH301 pKa = 7.14GISAARR307 pKa = 11.84HH308 pKa = 4.88ATDD311 pKa = 4.09LSYY314 pKa = 11.34SAVEE318 pKa = 4.86HH319 pKa = 6.11GWLMCICSVIPATGYY334 pKa = 9.33YY335 pKa = 9.91QGIPKK340 pKa = 8.63MWSRR344 pKa = 11.84FDD346 pKa = 3.53RR347 pKa = 11.84FDD349 pKa = 3.63YY350 pKa = 10.58AWPEE354 pKa = 3.85FAHH357 pKa = 7.12LGEE360 pKa = 4.23QPILNQEE367 pKa = 4.47LYY369 pKa = 10.77YY370 pKa = 10.44DD371 pKa = 4.06TASVDD376 pKa = 3.54PDD378 pKa = 3.63TGNTATWGYY387 pKa = 9.76LPRR390 pKa = 11.84YY391 pKa = 7.63TEE393 pKa = 4.53YY394 pKa = 10.54KK395 pKa = 9.43SQMNRR400 pKa = 11.84VAGDD404 pKa = 3.33FRR406 pKa = 11.84TSLDD410 pKa = 3.3YY411 pKa = 10.5WHH413 pKa = 7.71LARR416 pKa = 11.84KK417 pKa = 8.18FASLPALAQEE427 pKa = 4.58FLEE430 pKa = 4.53ISQGADD436 pKa = 2.82MDD438 pKa = 4.97RR439 pKa = 11.84IFAVIDD445 pKa = 3.32PTVQHH450 pKa = 6.35VLVHH454 pKa = 6.47WFHH457 pKa = 7.14AIKK460 pKa = 10.31VHH462 pKa = 6.35RR463 pKa = 11.84ALPRR467 pKa = 11.84IVTPDD472 pKa = 3.13LL473 pKa = 3.94

Molecular weight: 52.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2UG46|A0A0G2UG46_9VIRU Uncharacterized protein OS=Microviridae Fen685_11 OX=1655657 PE=4 SV=1
MM1 pKa = 7.57IFFIVLIVVTSSSLRR16 pKa = 11.84TALLIGLQSLFRR28 pKa = 11.84VSLLLSVIFLLAFLVDD44 pKa = 4.64KK45 pKa = 11.23LFLQLPHH52 pKa = 7.49RR53 pKa = 11.84LRR55 pKa = 11.84ILMLTCPSLIIWIPLTVLLLLVVIVSALRR84 pKa = 11.84LWNRR88 pKa = 11.84RR89 pKa = 3.35

Molecular weight: 10.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1322

89

473

264.4

29.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.985 ± 2.404

1.059 ± 0.747

6.203 ± 0.836

3.328 ± 0.57

4.085 ± 0.668

5.295 ± 1.196

2.269 ± 0.74

4.917 ± 0.742

3.858 ± 1.106

9.834 ± 2.091

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.345 ± 0.304

4.766 ± 1.5

4.992 ± 0.674

5.446 ± 1.186

6.278 ± 1.361

7.035 ± 0.602

5.976 ± 0.489

6.43 ± 0.456

2.118 ± 0.658

3.782 ± 0.768
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski