Amino acid dipepetide frequency for Bacillus phage QCM11

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.548AlaAla: 0.548 ± 0.251
1.233AlaCys: 1.233 ± 0.44
2.193AlaAsp: 2.193 ± 0.665
4.248AlaGlu: 4.248 ± 1.11
2.467AlaPhe: 2.467 ± 0.651
3.837AlaGly: 3.837 ± 0.858
0.137AlaHis: 0.137 ± 0.108
2.33AlaIle: 2.33 ± 0.668
4.111AlaLys: 4.111 ± 0.773
2.604AlaLeu: 2.604 ± 0.488
1.919AlaMet: 1.919 ± 0.561
2.056AlaAsn: 2.056 ± 0.506
1.37AlaPro: 1.37 ± 0.65
2.467AlaGln: 2.467 ± 0.563
1.507AlaArg: 1.507 ± 0.32
2.467AlaSer: 2.467 ± 0.62
3.015AlaThr: 3.015 ± 0.659
2.604AlaVal: 2.604 ± 0.935
0.685AlaTrp: 0.685 ± 0.287
2.193AlaTyr: 2.193 ± 0.508
0.0AlaXaa: 0.0 ± 0.0
Cys
0.274CysAla: 0.274 ± 0.216
0.137CysCys: 0.137 ± 0.137
1.645CysAsp: 1.645 ± 0.528
1.37CysGlu: 1.37 ± 0.419
0.411CysPhe: 0.411 ± 0.23
1.096CysGly: 1.096 ± 0.493
0.137CysHis: 0.137 ± 0.124
0.685CysIle: 0.685 ± 0.313
1.782CysLys: 1.782 ± 0.591
0.548CysLeu: 0.548 ± 0.264
0.411CysMet: 0.411 ± 0.227
0.411CysAsn: 0.411 ± 0.215
0.685CysPro: 0.685 ± 0.397
0.0CysGln: 0.0 ± 0.0
0.548CysArg: 0.548 ± 0.274
0.411CysSer: 0.411 ± 0.22
0.274CysThr: 0.274 ± 0.249
0.548CysVal: 0.548 ± 0.288
0.274CysTrp: 0.274 ± 0.165
0.685CysTyr: 0.685 ± 0.248
0.0CysXaa: 0.0 ± 0.0
Asp
2.741AspAla: 2.741 ± 0.56
1.233AspCys: 1.233 ± 0.645
3.015AspAsp: 3.015 ± 0.674
5.756AspGlu: 5.756 ± 0.878
4.385AspPhe: 4.385 ± 0.613
5.619AspGly: 5.619 ± 1.339
0.685AspHis: 0.685 ± 0.367
5.756AspIle: 5.756 ± 0.862
5.482AspLys: 5.482 ± 0.634
3.426AspLeu: 3.426 ± 0.744
2.056AspMet: 2.056 ± 0.56
4.934AspAsn: 4.934 ± 0.768
2.878AspPro: 2.878 ± 0.724
2.193AspGln: 2.193 ± 0.514
1.782AspArg: 1.782 ± 0.53
2.193AspSer: 2.193 ± 0.626
3.152AspThr: 3.152 ± 0.859
5.482AspVal: 5.482 ± 0.847
0.548AspTrp: 0.548 ± 0.21
3.015AspTyr: 3.015 ± 0.648
0.0AspXaa: 0.0 ± 0.0
Glu
3.289GluAla: 3.289 ± 0.969
0.822GluCys: 0.822 ± 0.328
4.111GluAsp: 4.111 ± 0.62
7.126GluGlu: 7.126 ± 1.518
3.974GluPhe: 3.974 ± 0.907
4.659GluGly: 4.659 ± 0.784
0.822GluHis: 0.822 ± 0.288
5.619GluIle: 5.619 ± 0.931
7.126GluLys: 7.126 ± 1.57
9.319GluLeu: 9.319 ± 1.444
2.878GluMet: 2.878 ± 0.649
5.482GluAsn: 5.482 ± 0.865
0.959GluPro: 0.959 ± 0.376
3.015GluGln: 3.015 ± 0.636
3.426GluArg: 3.426 ± 0.854
5.208GluSer: 5.208 ± 0.896
4.385GluThr: 4.385 ± 0.922
5.756GluVal: 5.756 ± 0.986
0.959GluTrp: 0.959 ± 0.339
3.974GluTyr: 3.974 ± 0.83
0.0GluXaa: 0.0 ± 0.0
Phe
1.919PheAla: 1.919 ± 0.464
0.548PheCys: 0.548 ± 0.239
3.7PheAsp: 3.7 ± 0.732
3.563PheGlu: 3.563 ± 0.694
1.782PhePhe: 1.782 ± 0.433
1.782PheGly: 1.782 ± 0.58
0.822PheHis: 0.822 ± 0.334
4.796PheIle: 4.796 ± 0.625
4.796PheLys: 4.796 ± 0.845
2.193PheLeu: 2.193 ± 0.488
1.645PheMet: 1.645 ± 0.496
2.604PheAsn: 2.604 ± 0.579
0.822PhePro: 0.822 ± 0.374
0.822PheGln: 0.822 ± 0.358
0.685PheArg: 0.685 ± 0.289
3.7PheSer: 3.7 ± 0.787
2.741PheThr: 2.741 ± 0.704
2.604PheVal: 2.604 ± 0.627
0.411PheTrp: 0.411 ± 0.186
2.33PheTyr: 2.33 ± 0.617
0.0PheXaa: 0.0 ± 0.0
Gly
3.289GlyAla: 3.289 ± 0.93
0.274GlyCys: 0.274 ± 0.171
3.015GlyAsp: 3.015 ± 0.714
5.345GlyGlu: 5.345 ± 0.709
3.289GlyPhe: 3.289 ± 0.563
3.7GlyGly: 3.7 ± 0.835
0.685GlyHis: 0.685 ± 0.34
4.385GlyIle: 4.385 ± 0.904
6.852GlyLys: 6.852 ± 1.07
4.522GlyLeu: 4.522 ± 0.65
2.604GlyMet: 2.604 ± 0.64
4.248GlyAsn: 4.248 ± 1.006
0.274GlyPro: 0.274 ± 0.149
1.645GlyGln: 1.645 ± 0.402
2.056GlyArg: 2.056 ± 0.486
4.111GlySer: 4.111 ± 0.721
5.893GlyThr: 5.893 ± 1.252
4.111GlyVal: 4.111 ± 0.582
0.274GlyTrp: 0.274 ± 0.162
3.289GlyTyr: 3.289 ± 0.66
0.0GlyXaa: 0.0 ± 0.0
His
0.822HisAla: 0.822 ± 0.297
0.137HisCys: 0.137 ± 0.125
1.096HisAsp: 1.096 ± 0.453
0.548HisGlu: 0.548 ± 0.261
0.959HisPhe: 0.959 ± 0.317
0.274HisGly: 0.274 ± 0.184
0.548HisHis: 0.548 ± 0.297
1.507HisIle: 1.507 ± 0.44
1.096HisLys: 1.096 ± 0.42
1.233HisLeu: 1.233 ± 0.456
0.137HisMet: 0.137 ± 0.126
0.548HisAsn: 0.548 ± 0.204
0.137HisPro: 0.137 ± 0.108
0.411HisGln: 0.411 ± 0.237
0.411HisArg: 0.411 ± 0.212
0.411HisSer: 0.411 ± 0.274
1.096HisThr: 1.096 ± 0.387
0.959HisVal: 0.959 ± 0.437
0.137HisTrp: 0.137 ± 0.123
0.822HisTyr: 0.822 ± 0.314
0.0HisXaa: 0.0 ± 0.0
Ile
3.152IleAla: 3.152 ± 0.566
0.685IleCys: 0.685 ± 0.342
6.578IleAsp: 6.578 ± 0.635
7.126IleGlu: 7.126 ± 1.332
2.056IlePhe: 2.056 ± 0.464
4.385IleGly: 4.385 ± 0.705
1.37IleHis: 1.37 ± 0.437
5.208IleIle: 5.208 ± 1.081
7.263IleLys: 7.263 ± 1.174
2.33IleLeu: 2.33 ± 0.598
2.741IleMet: 2.741 ± 0.582
4.934IleAsn: 4.934 ± 0.597
1.507IlePro: 1.507 ± 0.361
3.015IleGln: 3.015 ± 0.534
3.974IleArg: 3.974 ± 0.785
3.289IleSer: 3.289 ± 0.71
4.522IleThr: 4.522 ± 0.719
3.563IleVal: 3.563 ± 0.673
0.685IleTrp: 0.685 ± 0.28
2.33IleTyr: 2.33 ± 0.54
0.0IleXaa: 0.0 ± 0.0
Lys
4.111LysAla: 4.111 ± 1.108
1.507LysCys: 1.507 ± 0.565
7.126LysAsp: 7.126 ± 1.336
9.73LysGlu: 9.73 ± 1.537
3.837LysPhe: 3.837 ± 0.708
7.126LysGly: 7.126 ± 0.994
1.37LysHis: 1.37 ± 0.399
5.756LysIle: 5.756 ± 0.926
11.1LysLys: 11.1 ± 1.456
7.537LysLeu: 7.537 ± 1.084
3.289LysMet: 3.289 ± 0.71
5.756LysAsn: 5.756 ± 0.893
1.233LysPro: 1.233 ± 0.504
2.741LysGln: 2.741 ± 0.672
4.796LysArg: 4.796 ± 0.994
3.974LysSer: 3.974 ± 0.706
5.619LysThr: 5.619 ± 0.858
6.03LysVal: 6.03 ± 0.869
0.685LysTrp: 0.685 ± 0.318
4.111LysTyr: 4.111 ± 0.983
0.0LysXaa: 0.0 ± 0.0
Leu
2.604LeuAla: 2.604 ± 0.544
0.685LeuCys: 0.685 ± 0.304
4.934LeuAsp: 4.934 ± 0.817
5.619LeuGlu: 5.619 ± 1.048
2.467LeuPhe: 2.467 ± 0.764
3.974LeuGly: 3.974 ± 0.772
1.645LeuHis: 1.645 ± 0.447
4.385LeuIle: 4.385 ± 0.823
7.674LeuLys: 7.674 ± 1.459
4.522LeuLeu: 4.522 ± 0.681
1.919LeuMet: 1.919 ± 0.485
3.974LeuAsn: 3.974 ± 0.616
1.919LeuPro: 1.919 ± 0.368
3.015LeuGln: 3.015 ± 0.651
2.878LeuArg: 2.878 ± 0.624
3.974LeuSer: 3.974 ± 0.656
3.974LeuThr: 3.974 ± 0.615
3.7LeuVal: 3.7 ± 0.75
0.959LeuTrp: 0.959 ± 0.374
3.015LeuTyr: 3.015 ± 0.44
0.0LeuXaa: 0.0 ± 0.0
Met
0.822MetAla: 0.822 ± 0.262
0.822MetCys: 0.822 ± 0.336
2.193MetAsp: 2.193 ± 0.535
2.467MetGlu: 2.467 ± 0.608
1.919MetPhe: 1.919 ± 0.575
1.919MetGly: 1.919 ± 0.465
0.137MetHis: 0.137 ± 0.125
1.37MetIle: 1.37 ± 0.426
2.604MetLys: 2.604 ± 0.513
2.878MetLeu: 2.878 ± 0.631
1.507MetMet: 1.507 ± 0.575
3.837MetAsn: 3.837 ± 0.668
0.548MetPro: 0.548 ± 0.238
0.822MetGln: 0.822 ± 0.313
1.507MetArg: 1.507 ± 0.416
2.467MetSer: 2.467 ± 0.59
1.507MetThr: 1.507 ± 0.369
1.096MetVal: 1.096 ± 0.338
1.37MetTrp: 1.37 ± 0.42
1.919MetTyr: 1.919 ± 0.486
0.0MetXaa: 0.0 ± 0.0
Asn
2.193AsnAla: 2.193 ± 0.694
0.959AsnCys: 0.959 ± 0.377
4.522AsnAsp: 4.522 ± 0.765
5.345AsnGlu: 5.345 ± 0.709
2.878AsnPhe: 2.878 ± 0.586
4.111AsnGly: 4.111 ± 0.803
0.548AsnHis: 0.548 ± 0.259
4.659AsnIle: 4.659 ± 0.563
5.893AsnLys: 5.893 ± 0.987
4.522AsnLeu: 4.522 ± 0.822
1.37AsnMet: 1.37 ± 0.472
6.441AsnAsn: 6.441 ± 1.284
2.467AsnPro: 2.467 ± 0.702
2.467AsnGln: 2.467 ± 0.506
2.604AsnArg: 2.604 ± 0.657
4.796AsnSer: 4.796 ± 0.713
4.111AsnThr: 4.111 ± 1.138
4.934AsnVal: 4.934 ± 0.823
0.822AsnTrp: 0.822 ± 0.276
3.015AsnTyr: 3.015 ± 0.594
0.0AsnXaa: 0.0 ± 0.0
Pro
0.685ProAla: 0.685 ± 0.336
0.274ProCys: 0.274 ± 0.161
1.782ProAsp: 1.782 ± 0.491
1.37ProGlu: 1.37 ± 0.437
1.096ProPhe: 1.096 ± 0.492
0.685ProGly: 0.685 ± 0.274
0.137ProHis: 0.137 ± 0.128
1.782ProIle: 1.782 ± 0.464
2.056ProLys: 2.056 ± 0.565
1.096ProLeu: 1.096 ± 0.374
1.37ProMet: 1.37 ± 0.475
1.645ProAsn: 1.645 ± 0.56
0.822ProPro: 0.822 ± 0.285
0.822ProGln: 0.822 ± 0.283
0.685ProArg: 0.685 ± 0.315
1.919ProSer: 1.919 ± 0.484
1.919ProThr: 1.919 ± 0.502
1.782ProVal: 1.782 ± 0.513
0.274ProTrp: 0.274 ± 0.184
1.782ProTyr: 1.782 ± 0.656
0.0ProXaa: 0.0 ± 0.0
Gln
1.096GlnAla: 1.096 ± 0.416
0.137GlnCys: 0.137 ± 0.124
1.507GlnAsp: 1.507 ± 0.483
1.507GlnGlu: 1.507 ± 0.564
1.233GlnPhe: 1.233 ± 0.402
2.193GlnGly: 2.193 ± 0.605
0.0GlnHis: 0.0 ± 0.0
2.056GlnIle: 2.056 ± 0.49
2.604GlnLys: 2.604 ± 0.539
4.248GlnLeu: 4.248 ± 0.815
0.411GlnMet: 0.411 ± 0.254
2.467GlnAsn: 2.467 ± 0.376
1.096GlnPro: 1.096 ± 0.317
0.548GlnGln: 0.548 ± 0.263
1.37GlnArg: 1.37 ± 0.332
2.056GlnSer: 2.056 ± 0.583
2.604GlnThr: 2.604 ± 0.427
2.193GlnVal: 2.193 ± 0.632
0.411GlnTrp: 0.411 ± 0.211
2.741GlnTyr: 2.741 ± 0.545
0.0GlnXaa: 0.0 ± 0.0
Arg
3.289ArgAla: 3.289 ± 0.492
0.411ArgCys: 0.411 ± 0.232
2.056ArgAsp: 2.056 ± 0.48
3.152ArgGlu: 3.152 ± 0.727
2.33ArgPhe: 2.33 ± 0.531
2.33ArgGly: 2.33 ± 0.682
0.274ArgHis: 0.274 ± 0.19
1.782ArgIle: 1.782 ± 0.516
4.659ArgLys: 4.659 ± 0.972
2.467ArgLeu: 2.467 ± 0.71
1.507ArgMet: 1.507 ± 0.453
2.193ArgAsn: 2.193 ± 0.492
0.685ArgPro: 0.685 ± 0.279
1.507ArgGln: 1.507 ± 0.462
2.056ArgArg: 2.056 ± 0.552
1.919ArgSer: 1.919 ± 0.591
2.056ArgThr: 2.056 ± 0.522
1.645ArgVal: 1.645 ± 0.561
0.274ArgTrp: 0.274 ± 0.165
2.467ArgTyr: 2.467 ± 0.711
0.0ArgXaa: 0.0 ± 0.0
Ser
2.878SerAla: 2.878 ± 0.6
0.822SerCys: 0.822 ± 0.352
3.426SerAsp: 3.426 ± 0.711
4.248SerGlu: 4.248 ± 0.873
2.193SerPhe: 2.193 ± 0.545
3.426SerGly: 3.426 ± 0.546
1.096SerHis: 1.096 ± 0.343
4.522SerIle: 4.522 ± 0.957
3.837SerLys: 3.837 ± 0.778
3.7SerLeu: 3.7 ± 0.793
2.056SerMet: 2.056 ± 0.537
5.208SerAsn: 5.208 ± 0.963
1.096SerPro: 1.096 ± 0.338
2.056SerGln: 2.056 ± 0.549
2.193SerArg: 2.193 ± 0.508
2.741SerSer: 2.741 ± 0.759
3.289SerThr: 3.289 ± 0.677
3.152SerVal: 3.152 ± 0.669
0.0SerTrp: 0.0 ± 0.0
3.152SerTyr: 3.152 ± 0.635
0.0SerXaa: 0.0 ± 0.0
Thr
3.152ThrAla: 3.152 ± 1.012
0.411ThrCys: 0.411 ± 0.217
3.7ThrAsp: 3.7 ± 0.928
4.111ThrGlu: 4.111 ± 0.694
2.33ThrPhe: 2.33 ± 0.568
4.522ThrGly: 4.522 ± 1.264
0.822ThrHis: 0.822 ± 0.329
6.03ThrIle: 6.03 ± 0.647
6.167ThrLys: 6.167 ± 0.831
5.071ThrLeu: 5.071 ± 1.014
2.193ThrMet: 2.193 ± 0.405
3.563ThrAsn: 3.563 ± 0.702
1.645ThrPro: 1.645 ± 0.409
1.37ThrGln: 1.37 ± 0.368
2.193ThrArg: 2.193 ± 0.482
4.248ThrSer: 4.248 ± 0.794
4.522ThrThr: 4.522 ± 1.387
4.111ThrVal: 4.111 ± 0.801
0.274ThrTrp: 0.274 ± 0.185
1.507ThrTyr: 1.507 ± 0.521
0.0ThrXaa: 0.0 ± 0.0
Val
3.289ValAla: 3.289 ± 0.697
0.137ValCys: 0.137 ± 0.137
5.482ValAsp: 5.482 ± 0.936
5.756ValGlu: 5.756 ± 0.684
1.919ValPhe: 1.919 ± 0.45
4.796ValGly: 4.796 ± 0.575
1.096ValHis: 1.096 ± 0.38
4.248ValIle: 4.248 ± 1.044
6.578ValLys: 6.578 ± 0.843
3.015ValLeu: 3.015 ± 0.568
1.782ValMet: 1.782 ± 0.526
3.563ValAsn: 3.563 ± 0.751
2.467ValPro: 2.467 ± 0.663
1.645ValGln: 1.645 ± 0.493
2.056ValArg: 2.056 ± 0.451
3.152ValSer: 3.152 ± 0.605
4.385ValThr: 4.385 ± 1.077
4.796ValVal: 4.796 ± 0.925
0.959ValTrp: 0.959 ± 0.396
2.33ValTyr: 2.33 ± 0.594
0.0ValXaa: 0.0 ± 0.0
Trp
0.274TrpAla: 0.274 ± 0.198
0.274TrpCys: 0.274 ± 0.17
0.274TrpAsp: 0.274 ± 0.155
0.548TrpGlu: 0.548 ± 0.263
1.096TrpPhe: 1.096 ± 0.354
0.548TrpGly: 0.548 ± 0.265
0.548TrpHis: 0.548 ± 0.248
0.411TrpIle: 0.411 ± 0.224
1.233TrpLys: 1.233 ± 0.39
0.685TrpLeu: 0.685 ± 0.317
0.411TrpMet: 0.411 ± 0.218
1.507TrpAsn: 1.507 ± 0.433
0.0TrpPro: 0.0 ± 0.0
0.685TrpGln: 0.685 ± 0.251
0.548TrpArg: 0.548 ± 0.234
0.274TrpSer: 0.274 ± 0.18
0.274TrpThr: 0.274 ± 0.175
0.822TrpVal: 0.822 ± 0.345
0.274TrpTrp: 0.274 ± 0.252
0.685TrpTyr: 0.685 ± 0.272
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.563TyrAla: 3.563 ± 0.61
0.822TyrCys: 0.822 ± 0.376
3.974TyrAsp: 3.974 ± 0.624
3.974TyrGlu: 3.974 ± 0.765
1.645TyrPhe: 1.645 ± 0.354
2.878TyrGly: 2.878 ± 0.587
0.411TyrHis: 0.411 ± 0.207
3.426TyrIle: 3.426 ± 0.779
4.659TyrLys: 4.659 ± 0.775
1.919TyrLeu: 1.919 ± 0.481
1.37TyrMet: 1.37 ± 0.499
3.289TyrAsn: 3.289 ± 0.62
1.37TyrPro: 1.37 ± 0.401
1.233TyrGln: 1.233 ± 0.31
1.782TyrArg: 1.782 ± 0.505
1.782TyrSer: 1.782 ± 0.495
2.604TyrThr: 2.604 ± 0.591
3.563TyrVal: 3.563 ± 0.617
1.096TyrTrp: 1.096 ± 0.503
1.919TyrTyr: 1.919 ± 0.473
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 41 proteins (7298 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski