Penicillium aurantiogriseum partitivirus 1
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S2KQ39|A0A0S2KQ39_9VIRU 47 kDa protein OS=Penicillium aurantiogriseum partitivirus 1 OX=1756157 PE=4 SV=1
MM1 pKa = 7.65 PRR3 pKa = 11.84 QPSEE7 pKa = 4.31 VPSTLAPSDD16 pKa = 3.77 SASAAGSKK24 pKa = 9.95 RR25 pKa = 11.84 SKK27 pKa = 9.81 PGKK30 pKa = 9.73 AEE32 pKa = 3.64 RR33 pKa = 11.84 AARR36 pKa = 11.84 RR37 pKa = 11.84 ATGSQAPKK45 pKa = 9.75 PADD48 pKa = 3.37 AGKK51 pKa = 10.96 AMTFASGGMIPKK63 pKa = 8.45 PQPGKK68 pKa = 10.58 FPVVFQTGAGEE79 pKa = 3.96 PSRR82 pKa = 11.84 DD83 pKa = 3.2 QGFAIEE89 pKa = 4.5 PNVLADD95 pKa = 3.84 TVSQFPSRR103 pKa = 11.84 FTSNAGYY110 pKa = 11.13 AEE112 pKa = 4.25 FLTFTDD118 pKa = 5.24 LDD120 pKa = 4.32 DD121 pKa = 6.72 DD122 pKa = 5.53 DD123 pKa = 4.79 FAKK126 pKa = 10.37 QLKK129 pKa = 9.61 SASLLRR135 pKa = 11.84 LAQQLVHH142 pKa = 6.26 AHH144 pKa = 5.33 VNMGLPQGDD153 pKa = 4.3 FAPVASTEE161 pKa = 3.93 VRR163 pKa = 11.84 VPASVNAYY171 pKa = 9.54 LSQFGEE177 pKa = 4.24 FSVPALGTRR186 pKa = 11.84 FLFDD190 pKa = 5.13 DD191 pKa = 3.84 YY192 pKa = 11.82 ASTVKK197 pKa = 10.69 SIIHH201 pKa = 5.37 TAEE204 pKa = 3.68 QLLAASNDD212 pKa = 3.4 DD213 pKa = 3.66 SEE215 pKa = 7.05 AILKK219 pKa = 9.94 RR220 pKa = 11.84 CWLPMSRR227 pKa = 11.84 TDD229 pKa = 2.71 GHH231 pKa = 5.59 TRR233 pKa = 11.84 QIIANYY239 pKa = 8.93 LNQWLLTNEE248 pKa = 3.73 IMYY251 pKa = 8.33 PAQLLEE257 pKa = 4.18 KK258 pKa = 9.8 AVMSGTPPAAWDD270 pKa = 3.68 KK271 pKa = 10.56 IKK273 pKa = 10.66 PVFGDD278 pKa = 3.47 TEE280 pKa = 4.37 TEE282 pKa = 3.84 KK283 pKa = 11.04 NRR285 pKa = 11.84 FDD287 pKa = 4.14 FLFSGYY293 pKa = 9.57 RR294 pKa = 11.84 DD295 pKa = 3.47 APHH298 pKa = 6.7 FVTTFTTAASSAVLEE313 pKa = 4.62 EE314 pKa = 5.71 IGLSWDD320 pKa = 3.71 NPSAAHH326 pKa = 6.71 VDD328 pKa = 3.04 WTFNAKK334 pKa = 9.97 EE335 pKa = 4.21 SFTRR339 pKa = 11.84 LSDD342 pKa = 2.68 NWARR346 pKa = 11.84 ISTTYY351 pKa = 11.02 GRR353 pKa = 11.84 FFEE356 pKa = 4.63 MPSSQMNRR364 pKa = 11.84 SAATGSQSQMAIVSSKK380 pKa = 11.3 DD381 pKa = 3.01 SVTVVKK387 pKa = 9.0 THH389 pKa = 6.99 LALSAPEE396 pKa = 4.16 FSLVACFPATCLFSGDD412 pKa = 3.52 LRR414 pKa = 11.84 RR415 pKa = 11.84 HH416 pKa = 4.79 VVVTTPLSTVQRR428 pKa = 11.84 ATEE431 pKa = 4.42 FVQLDD436 pKa = 3.41 WRR438 pKa = 4.22
Molecular weight: 47.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.051
IPC2_protein 5.919
IPC_protein 5.995
Toseland 6.415
ProMoST 6.376
Dawson 6.287
Bjellqvist 6.262
Wikipedia 6.3
Rodwell 6.275
Grimsley 6.576
Solomon 6.287
Lehninger 6.275
Nozaki 6.547
DTASelect 6.737
Thurlkill 6.751
EMBOSS 6.722
Sillero 6.649
Patrickios 4.596
IPC_peptide 6.3
IPC2_peptide 6.59
IPC2.peptide.svr19 6.601
Protein with the highest isoelectric point:
>tr|A0A0S2KQ39|A0A0S2KQ39_9VIRU 47 kDa protein OS=Penicillium aurantiogriseum partitivirus 1 OX=1756157 PE=4 SV=1
MM1 pKa = 7.9 AFTQEE6 pKa = 4.1 PTQHH10 pKa = 5.69 YY11 pKa = 9.06 VLAKK15 pKa = 9.8 GSHH18 pKa = 6.8 LIDD21 pKa = 3.96 SLHH24 pKa = 6.47 LRR26 pKa = 11.84 PAKK29 pKa = 10.42 AGSATSEE36 pKa = 4.2 DD37 pKa = 4.2 VLPTGYY43 pKa = 10.64 NSPNLRR49 pKa = 11.84 EE50 pKa = 3.64 IAKK53 pKa = 10.08 FGGYY57 pKa = 7.52 STYY60 pKa = 11.18 SSASNTDD67 pKa = 2.61 PWVRR71 pKa = 11.84 EE72 pKa = 3.87 TLKK75 pKa = 10.74 LFSRR79 pKa = 11.84 EE80 pKa = 3.86 RR81 pKa = 11.84 YY82 pKa = 8.44 EE83 pKa = 4.06 EE84 pKa = 3.99 IYY86 pKa = 10.92 GFTRR90 pKa = 11.84 RR91 pKa = 11.84 PEE93 pKa = 4.26 GTPGMYY99 pKa = 10.1 KK100 pKa = 10.27 SLAKK104 pKa = 10.14 FAGEE108 pKa = 3.83 KK109 pKa = 9.93 SHH111 pKa = 7.31 FRR113 pKa = 11.84 DD114 pKa = 3.75 LTVSQQKK121 pKa = 10.56 AMRR124 pKa = 11.84 RR125 pKa = 11.84 SIAKK129 pKa = 9.5 AKK131 pKa = 10.33 KK132 pKa = 9.94 AFKK135 pKa = 10.34 LPYY138 pKa = 9.55 KK139 pKa = 10.34 RR140 pKa = 11.84 EE141 pKa = 3.91 PLDD144 pKa = 3.15 WHH146 pKa = 6.48 EE147 pKa = 4.21 VGQFLRR153 pKa = 11.84 RR154 pKa = 11.84 DD155 pKa = 3.54 TSAGSTFMGQKK166 pKa = 10.07 KK167 pKa = 9.93 GDD169 pKa = 3.65 VMEE172 pKa = 5.9 DD173 pKa = 2.98 IYY175 pKa = 11.47 HH176 pKa = 6.14 EE177 pKa = 4.38 ARR179 pKa = 11.84 WLGHH183 pKa = 6.06 RR184 pKa = 11.84 MKK186 pKa = 10.62 QDD188 pKa = 3.31 GEE190 pKa = 4.54 SSFNPTKK197 pKa = 10.28 MRR199 pKa = 11.84 FPPCLAGQRR208 pKa = 11.84 GGMSEE213 pKa = 4.57 RR214 pKa = 11.84 DD215 pKa = 3.57 DD216 pKa = 3.99 PKK218 pKa = 11.09 TRR220 pKa = 11.84 LVWIYY225 pKa = 8.98 PAEE228 pKa = 3.96 MLVIEE233 pKa = 5.06 GFYY236 pKa = 10.8 APLMYY241 pKa = 9.7 RR242 pKa = 11.84 DD243 pKa = 4.6 FMNDD247 pKa = 2.96 PNSPMLNGKK256 pKa = 8.05 SAPRR260 pKa = 11.84 LYY262 pKa = 11.01 AEE264 pKa = 4.49 WCCGLRR270 pKa = 11.84 EE271 pKa = 4.19 GEE273 pKa = 4.23 TLYY276 pKa = 11.53 GLDD279 pKa = 4.6 FSAFDD284 pKa = 3.67 TKK286 pKa = 11.08 VPTWLIYY293 pKa = 9.48 TAFDD297 pKa = 3.36 ILRR300 pKa = 11.84 QNIEE304 pKa = 3.32 WSTFQGKK311 pKa = 8.11 PVSKK315 pKa = 10.53 QDD317 pKa = 3.2 AQKK320 pKa = 9.9 WRR322 pKa = 11.84 NVWDD326 pKa = 3.59 GMVWYY331 pKa = 9.07 FVNTPILMPDD341 pKa = 2.41 GRR343 pKa = 11.84 MFRR346 pKa = 11.84 KK347 pKa = 9.98 YY348 pKa = 10.14 RR349 pKa = 11.84 GVPSGSWWTQMIDD362 pKa = 3.49 SVVNYY367 pKa = 10.26 ILIDD371 pKa = 3.69 YY372 pKa = 9.7 LAEE375 pKa = 4.23 CQEE378 pKa = 4.02 VEE380 pKa = 3.92 IRR382 pKa = 11.84 NLRR385 pKa = 11.84 VLGDD389 pKa = 3.68 DD390 pKa = 3.44 SAFRR394 pKa = 11.84 STDD397 pKa = 3.29 QFSLEE402 pKa = 4.02 QAKK405 pKa = 10.08 VDD407 pKa = 4.07 CEE409 pKa = 4.19 PTHH412 pKa = 6.37 MLLKK416 pKa = 10.35 PEE418 pKa = 4.25 KK419 pKa = 10.11 CEE421 pKa = 3.96 KK422 pKa = 10.32 TKK424 pKa = 10.91 DD425 pKa = 3.32 PCEE428 pKa = 4.01 FKK430 pKa = 11.23 LLGTTYY436 pKa = 10.79 RR437 pKa = 11.84 DD438 pKa = 3.59 GRR440 pKa = 11.84 VHH442 pKa = 7.37 RR443 pKa = 11.84 PTEE446 pKa = 3.75 EE447 pKa = 3.53 WFKK450 pKa = 11.17 LVIYY454 pKa = 9.67 PEE456 pKa = 4.57 SSVHH460 pKa = 5.71 TLDD463 pKa = 3.85 ISFTRR468 pKa = 11.84 LIGLWLGGAMWDD480 pKa = 4.48 KK481 pKa = 10.62 EE482 pKa = 4.18 FCRR485 pKa = 11.84 YY486 pKa = 8.64 MDD488 pKa = 4.7 FFQSSYY494 pKa = 10.42 PCPEE498 pKa = 4.29 EE499 pKa = 4.24 GWFSKK504 pKa = 10.13 DD505 pKa = 3.02 QKK507 pKa = 10.68 RR508 pKa = 11.84 WLEE511 pKa = 3.93 VIYY514 pKa = 10.54 SGKK517 pKa = 10.27 APRR520 pKa = 11.84 GWTTKK525 pKa = 10.44 RR526 pKa = 11.84 SLFWRR531 pKa = 11.84 SIFYY535 pKa = 10.51 AYY537 pKa = 10.44 GG538 pKa = 3.3
Molecular weight: 62.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.693
IPC2_protein 7.98
IPC_protein 7.834
Toseland 7.732
ProMoST 8.375
Dawson 8.609
Bjellqvist 8.785
Wikipedia 8.609
Rodwell 8.653
Grimsley 7.732
Solomon 8.682
Lehninger 8.682
Nozaki 8.887
DTASelect 8.595
Thurlkill 8.653
EMBOSS 8.785
Sillero 8.916
Patrickios 4.38
IPC_peptide 8.682
IPC2_peptide 7.585
IPC2.peptide.svr19 7.849
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
976
438
538
488.0
55.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.402 ± 2.425
1.23 ± 0.357
5.738 ± 0.02
5.635 ± 0.851
5.943 ± 0.295
6.25 ± 0.655
1.844 ± 0.012
3.279 ± 0.353
5.533 ± 0.933
7.684 ± 0.098
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.971 ± 0.451
2.561 ± 0.267
6.148 ± 0.31
3.893 ± 0.441
6.352 ± 0.722
8.607 ± 1.093
6.66 ± 0.723
5.225 ± 0.765
2.664 ± 0.699
3.381 ± 1.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here