Hoeflea marina

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Hoeflea

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4798 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A317PT36|A0A317PT36_9RHIZ Uncharacterized protein OS=Hoeflea marina OX=274592 GN=DFR52_102649 PE=4 SV=1
MM1 pKa = 7.74APTLSVRR8 pKa = 11.84VALLRR13 pKa = 11.84HH14 pKa = 6.21GATSSCRR21 pKa = 11.84NAGVEE26 pKa = 3.99RR27 pKa = 11.84HH28 pKa = 5.7RR29 pKa = 11.84SLMRR33 pKa = 11.84LGVAGAALLAGAGAAAADD51 pKa = 3.84DD52 pKa = 4.12VSWINSSTVEE62 pKa = 4.02MRR64 pKa = 11.84WEE66 pKa = 4.74DD67 pKa = 3.35GGNWSGGTVPAQGDD81 pKa = 4.1TVTIATGNSGLYY93 pKa = 9.8TDD95 pKa = 4.07VTVEE99 pKa = 4.14TVNIEE104 pKa = 5.11SGTLWINRR112 pKa = 11.84NAEE115 pKa = 4.11LNADD119 pKa = 3.92TVEE122 pKa = 4.29VTGGRR127 pKa = 11.84LALSQGNPAAPNNSGPQGIANISTLLTISGGTVEE161 pKa = 4.77RR162 pKa = 11.84FGLIEE167 pKa = 4.91LGDD170 pKa = 4.35AGTVIQSGGEE180 pKa = 3.87VLNPVQIHH188 pKa = 5.2TPSYY192 pKa = 8.23VQTGGAMVGLVTASTYY208 pKa = 10.66AISGAEE214 pKa = 4.05GVAATHH220 pKa = 5.88MAGTVTLGTEE230 pKa = 3.95FTMADD235 pKa = 3.39GALVSGTVAGDD246 pKa = 3.64GNATMLQTGGTMDD259 pKa = 4.1GSASGLTSYY268 pKa = 10.93AQTGGTIQGQIGYY281 pKa = 8.01TEE283 pKa = 4.25TFDD286 pKa = 4.58LSGDD290 pKa = 3.77GTVVWGAQLVGDD302 pKa = 4.28SGAVFEE308 pKa = 4.65QSGGTMGGTVQFEE321 pKa = 4.39DD322 pKa = 3.93GAGISKK328 pKa = 9.85YY329 pKa = 9.6VQTGGEE335 pKa = 4.02MTGTVTTGTYY345 pKa = 7.83EE346 pKa = 3.88ASGGTIVVHH355 pKa = 5.97SNVGFSEE362 pKa = 4.36LFALSGDD369 pKa = 3.56ASLGDD374 pKa = 3.64IYY376 pKa = 11.08LIGDD380 pKa = 3.88GDD382 pKa = 4.02AAMTQSGGTMGAWVSGIEE400 pKa = 4.33TYY402 pKa = 11.2TMTGGTILQATEE414 pKa = 4.0VVTDD418 pKa = 4.14AFVQSGGTINDD429 pKa = 3.16ITEE432 pKa = 4.17EE433 pKa = 4.15NDD435 pKa = 2.49WRR437 pKa = 11.84ATVRR441 pKa = 11.84FATEE445 pKa = 3.98YY446 pKa = 10.19EE447 pKa = 4.07LSGDD451 pKa = 3.66ATIGLANLIGGADD464 pKa = 3.29ATMTQSGGTMGGHH477 pKa = 6.25VYY479 pKa = 10.69SEE481 pKa = 4.68TEE483 pKa = 3.73SEE485 pKa = 4.44TIAKK489 pKa = 7.38YY490 pKa = 7.91TQTGGDD496 pKa = 3.22MTGQVTTEE504 pKa = 3.97TYY506 pKa = 8.17EE507 pKa = 4.04ASGGTVSGSVVFSDD521 pKa = 3.98LFAMSGDD528 pKa = 3.45AVVGEE533 pKa = 4.94GYY535 pKa = 10.74SLIGDD540 pKa = 4.22GDD542 pKa = 4.23ALMTQSGGTMAGGVSGIATYY562 pKa = 10.47TMSGGTITGTVDD574 pKa = 3.74FTDD577 pKa = 3.51HH578 pKa = 7.18FEE580 pKa = 4.33LSDD583 pKa = 3.52GRR585 pKa = 11.84IEE587 pKa = 4.15YY588 pKa = 10.7VDD590 pKa = 3.24IVGDD594 pKa = 3.68IDD596 pKa = 3.56ATMTQSGGSMGGAVWSLDD614 pKa = 3.26TDD616 pKa = 4.06DD617 pKa = 4.0YY618 pKa = 11.58TIAKK622 pKa = 7.04YY623 pKa = 7.82TQTGGTMNAEE633 pKa = 4.43VYY635 pKa = 10.72ARR637 pKa = 11.84TYY639 pKa = 10.44DD640 pKa = 3.52VSGGSVDD647 pKa = 3.16GMVRR651 pKa = 11.84FSEE654 pKa = 4.47LFALSGDD661 pKa = 3.67ATVGDD666 pKa = 4.4HH667 pKa = 6.59YY668 pKa = 11.12IWGYY672 pKa = 8.69GAAVATQSGGTMGGVVSDD690 pKa = 3.27ITGYY694 pKa = 6.83TQSGGAMTNYY704 pKa = 9.9VATEE708 pKa = 4.41DD709 pKa = 3.99YY710 pKa = 10.59ALSGGSISGTGHH722 pKa = 6.64VDD724 pKa = 3.67FTDD727 pKa = 4.14LFTLSGSGEE736 pKa = 4.1VLAEE740 pKa = 4.15GTLSGDD746 pKa = 3.35TTAQMAQSGGTMSGHH761 pKa = 5.72VNGITGYY768 pKa = 6.9TQSDD772 pKa = 3.4GTMDD776 pKa = 3.91GKK778 pKa = 11.09VDD780 pKa = 3.43AADD783 pKa = 3.74YY784 pKa = 7.11TQSGGTMGGEE794 pKa = 3.96ATAQTYY800 pKa = 10.9ALAGGTLSGTANFGTSATLSGTGSVTSTGKK830 pKa = 10.97LIGDD834 pKa = 4.38GSATVAQTGGAMDD847 pKa = 3.83GTVTGVTGYY856 pKa = 7.06TQSGGTMGGEE866 pKa = 4.27TTAQTYY872 pKa = 10.39ALSGGTLSGTANFGTSAALSGTGSVTSTGQLIGDD906 pKa = 4.45GSATVAQSGGAMDD919 pKa = 4.04GTVSGVTGYY928 pKa = 7.18TQSGGTMGGKK938 pKa = 8.17VTTGSYY944 pKa = 10.4EE945 pKa = 3.88LLGGVLSGDD954 pKa = 3.66VTFSTLFALSGDD966 pKa = 4.08SVVTSSTQLTQTGGSMDD983 pKa = 3.55STLSGIASYY992 pKa = 8.31TQSGGAMGGDD1002 pKa = 3.74VTATTYY1008 pKa = 9.98TLSGGTLTGKK1018 pKa = 9.99VAGFSSFEE1026 pKa = 4.04LSGDD1030 pKa = 3.54PSDD1033 pKa = 4.87VIVTGTLIGASGSTVTQTGGKK1054 pKa = 8.93MNGNVSGIATYY1065 pKa = 10.09VQQEE1069 pKa = 4.42RR1070 pKa = 11.84VGSAAVISFSEE1081 pKa = 4.84LYY1083 pKa = 11.22DD1084 pKa = 3.6MVDD1087 pKa = 3.55GQVDD1091 pKa = 3.87TGAKK1095 pKa = 9.5IIGGAGSVMTQSGPGKK1111 pKa = 9.63MGGSVTGVASYY1122 pKa = 8.72TQTAGTMNGSVSTGDD1137 pKa = 3.51YY1138 pKa = 9.02TLSAGTVSKK1147 pKa = 9.48TVNFSGAFTLGSIDD1161 pKa = 4.0ANSAGQVNGSAVLTGGAGSTMTQSAGSMKK1190 pKa = 9.64GTVSGVSSYY1199 pKa = 9.6TQTDD1203 pKa = 3.25GTMGGAQDD1211 pKa = 4.09GKK1213 pKa = 10.1PNVIVGKK1220 pKa = 10.41VGTGTYY1226 pKa = 9.73DD1227 pKa = 3.24LSGGTVGGQVNFSTLFALSGTGDD1250 pKa = 3.55VASTATLTGGSGSTMTQSAGTMGGTVTGAATYY1282 pKa = 8.83AQSGGTMSGTVSTGSYY1298 pKa = 9.62EE1299 pKa = 3.99LLGGVLSGDD1308 pKa = 3.66VTFSTLFALSGDD1320 pKa = 3.63GVMTSSTRR1328 pKa = 11.84LTQTGGSMDD1337 pKa = 3.55STLSGIASYY1346 pKa = 8.74TQTGGTMSGATTAASYY1362 pKa = 9.26TLSAGTLSGDD1372 pKa = 3.78ANFSTLFALSGSGDD1386 pKa = 3.39VTAAAEE1392 pKa = 4.03LTGNGEE1398 pKa = 4.3AVMTQSGGTMAGTVTGVTSYY1418 pKa = 8.81TQSGGTMSGATTAASYY1434 pKa = 9.26TLSAGTLSGDD1444 pKa = 3.78ANFSTLFALSGSADD1458 pKa = 3.32VTAAAEE1464 pKa = 4.02LTGNGEE1470 pKa = 4.3AVMTQSGGTMAGTVTGIASYY1490 pKa = 9.79AQSGGTMSGATTAASYY1506 pKa = 9.28TLSAGILSGDD1516 pKa = 3.44ATFSTLLALSGTGGVTSTSQLTGGSGSTMTQSGGTMAGTVTGIASYY1562 pKa = 9.79AQSGGTMSGATTAASYY1578 pKa = 9.26TLSAGTLSGDD1588 pKa = 3.75ANFSTLLALSGTGGVTSTAQLTGGSGSTITQSAGTMAGTVTGIASYY1634 pKa = 8.12TQSGGTMTGATTAASYY1650 pKa = 9.26TLSAGTLSGDD1660 pKa = 3.67ADD1662 pKa = 4.31FSTLFSLSGTGEE1674 pKa = 3.68ISSAAEE1680 pKa = 3.76LTGSGDD1686 pKa = 3.49AAMTQSAGTMNGSVAGAKK1704 pKa = 9.45TYY1706 pKa = 7.49TQSGGTMSGDD1716 pKa = 3.0VAAASFALSGGTLSGDD1732 pKa = 3.2VDD1734 pKa = 4.56FSTLFSLSDD1743 pKa = 3.28TGSIASTALLAGGAGATMTQSGGTMDD1769 pKa = 3.66GTITGIDD1776 pKa = 3.9SYY1778 pKa = 8.91TQSGGTMTGDD1788 pKa = 3.35VTTLAYY1794 pKa = 10.61NHH1796 pKa = 6.4VGGSASGTIDD1806 pKa = 3.05VATYY1810 pKa = 10.37SLSGASASWSGTITASSAFNLGFDD1834 pKa = 4.32GGTATIGAKK1843 pKa = 10.44LSGAGDD1849 pKa = 3.85LVKK1852 pKa = 10.73SGTSTVVLANSANDD1866 pKa = 3.53FSGSVAINGGVLEE1879 pKa = 4.5VRR1881 pKa = 11.84DD1882 pKa = 3.48AALPGEE1888 pKa = 4.38ATVTVADD1895 pKa = 3.91SATLRR1900 pKa = 11.84FNTAAGVSVVFDD1912 pKa = 4.69GIMTGDD1918 pKa = 3.56GGILDD1923 pKa = 3.8KK1924 pKa = 11.2TGLGTLTLGGDD1935 pKa = 3.52IEE1937 pKa = 5.22LGALLVNGGVLNIGNGEE1954 pKa = 3.96PEE1956 pKa = 3.97EE1957 pKa = 5.23ASFQSAYY1964 pKa = 9.62IGVGATVYY1972 pKa = 9.9VASGATLRR1980 pKa = 11.84IRR1982 pKa = 11.84IPQNIANFGTLVNDD1996 pKa = 3.58GTVYY2000 pKa = 11.27DD2001 pKa = 4.84DD2002 pKa = 5.05LDD2004 pKa = 3.7NAGWFEE2010 pKa = 4.28NNALYY2015 pKa = 10.4VANLASNTHH2024 pKa = 7.09DD2025 pKa = 2.74IHH2027 pKa = 7.58NNLRR2031 pKa = 11.84WEE2033 pKa = 4.41GDD2035 pKa = 3.57VLTNNGWIFNNDD2047 pKa = 3.23DD2048 pKa = 3.77ATWVGDD2054 pKa = 3.61IVSNGPLDD2062 pKa = 3.88PNGRR2066 pKa = 11.84PGRR2069 pKa = 11.84IANAGGTWQGDD2080 pKa = 3.47ILSNAGHH2087 pKa = 7.63IINDD2091 pKa = 4.02NIADD2095 pKa = 3.99EE2096 pKa = 4.65SDD2098 pKa = 2.93FGYY2101 pKa = 10.25WFGDD2105 pKa = 3.04IYY2107 pKa = 11.63SNTNWIFNGGGGIWEE2122 pKa = 4.46GDD2124 pKa = 3.86VITNAGRR2131 pKa = 11.84IDD2133 pKa = 3.76NGKK2136 pKa = 9.01SAVWTGDD2143 pKa = 3.33VQSNASTVWNGGIWTGGVLGNAGTLHH2169 pKa = 6.37NAGGTWNGAVEE2180 pKa = 4.53ANTGLIINIAGSDD2193 pKa = 3.61ANGPTGGSTWIGDD2206 pKa = 3.59VTTNAGSILNAADD2219 pKa = 4.02SSWSGNVLANSGTIATLGLWTGDD2242 pKa = 3.21FSNAGRR2248 pKa = 11.84VVAEE2252 pKa = 4.17NEE2254 pKa = 3.98IIGAFSNSGTLRR2266 pKa = 11.84LSGDD2270 pKa = 3.36LSGITALSNSSTIDD2284 pKa = 3.09MKK2286 pKa = 11.31GRR2288 pKa = 11.84GVQVLSVASASFEE2301 pKa = 4.26SGSFLDD2307 pKa = 4.52VGLDD2311 pKa = 3.36SDD2313 pKa = 4.44GNSDD2317 pKa = 3.54QVAVAGLATLGGTVRR2332 pKa = 11.84ISNSGAAHH2340 pKa = 6.6GSGPFTFITAGSFSGSFDD2358 pKa = 4.11AVTTDD2363 pKa = 3.84LAFLAPQLSYY2373 pKa = 11.5GSGSVSVAVQRR2384 pKa = 11.84NDD2386 pKa = 2.77IGFSTTGVTGNQISTGTAVEE2406 pKa = 4.04ALAAGDD2412 pKa = 3.91AVYY2415 pKa = 10.73DD2416 pKa = 3.65AVLWLTEE2423 pKa = 4.38AEE2425 pKa = 4.14AQSAFDD2431 pKa = 3.51QLSGEE2436 pKa = 4.37VHH2438 pKa = 6.84ASHH2441 pKa = 7.16ASAAVEE2447 pKa = 3.99SAAMIGRR2454 pKa = 11.84MASNRR2459 pKa = 11.84IDD2461 pKa = 3.3KK2462 pKa = 10.88AFAAFGEE2469 pKa = 4.81TEE2471 pKa = 4.38ADD2473 pKa = 3.42AQPGSNAVWGRR2484 pKa = 11.84IYY2486 pKa = 10.7GARR2489 pKa = 11.84SLVEE2493 pKa = 4.7AGSQTAGAEE2502 pKa = 4.18TLNGGMVIGLDD2513 pKa = 3.28GMAGVWRR2520 pKa = 11.84LGLLLQGGTSEE2531 pKa = 4.3MEE2533 pKa = 3.6IGALDD2538 pKa = 3.75SSASSDD2544 pKa = 3.47DD2545 pKa = 3.85YY2546 pKa = 12.0GFGAYY2551 pKa = 9.7GGRR2554 pKa = 11.84QFGDD2558 pKa = 3.49TLVSFGAGYY2567 pKa = 8.17TRR2569 pKa = 11.84HH2570 pKa = 6.18DD2571 pKa = 3.59TSTTRR2576 pKa = 11.84QVDD2579 pKa = 3.7FTGFSDD2585 pKa = 3.92TLSADD2590 pKa = 3.83YY2591 pKa = 11.23ASGTAQAFAEE2601 pKa = 4.54VAHH2604 pKa = 6.66RR2605 pKa = 11.84FDD2607 pKa = 4.55LGAVSLTPFAGLAYY2621 pKa = 10.04VRR2623 pKa = 11.84HH2624 pKa = 5.56ATDD2627 pKa = 3.83AFTEE2631 pKa = 4.36TGGAAALSSAANVSEE2646 pKa = 4.12ATFASLGLGVEE2657 pKa = 4.33RR2658 pKa = 11.84QVTVADD2664 pKa = 4.07GMLLTASGSLGWRR2677 pKa = 11.84HH2678 pKa = 6.07AFAEE2682 pKa = 4.67TPISVQRR2689 pKa = 11.84FGSGLAFEE2697 pKa = 5.07ISGEE2701 pKa = 4.05PVASDD2706 pKa = 3.37MLTLGGGLALDD2717 pKa = 3.67IGGGTSLDD2725 pKa = 3.39LTYY2728 pKa = 10.85AGQIGSGAQTHH2739 pKa = 6.87ALQGTWSKK2747 pKa = 11.19RR2748 pKa = 11.84FF2749 pKa = 3.35

Molecular weight:
270.39 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A317PV17|A0A317PV17_9RHIZ DHA1 family inner membrane transport protein OS=Hoeflea marina OX=274592 GN=DFR52_1011233 PE=4 SV=1
MM1 pKa = 7.42TNQATGLSIATVAALIGDD19 pKa = 4.14VARR22 pKa = 11.84SNILHH27 pKa = 6.84ALMGGKK33 pKa = 9.92ALTATEE39 pKa = 4.4LAHH42 pKa = 6.28HH43 pKa = 7.02AGVSAPTTSGHH54 pKa = 6.72LARR57 pKa = 11.84LVDD60 pKa = 3.93GKK62 pKa = 10.89LVLMEE67 pKa = 3.68KK68 pKa = 10.01QGRR71 pKa = 11.84HH72 pKa = 4.43RR73 pKa = 11.84YY74 pKa = 8.7FRR76 pKa = 11.84LASPAVAEE84 pKa = 4.07ILEE87 pKa = 4.33GLGNLAAAGPARR99 pKa = 11.84HH100 pKa = 6.17RR101 pKa = 11.84PTGPKK106 pKa = 9.86DD107 pKa = 2.97LAMRR111 pKa = 11.84HH112 pKa = 5.84ARR114 pKa = 11.84TCYY117 pKa = 10.02DD118 pKa = 2.98HH119 pKa = 6.17MAGRR123 pKa = 11.84IAVALTDD130 pKa = 3.68SLTEE134 pKa = 3.82RR135 pKa = 11.84GLIIIEE141 pKa = 4.33DD142 pKa = 3.71RR143 pKa = 11.84SGLVTDD149 pKa = 4.19DD150 pKa = 3.16GRR152 pKa = 11.84RR153 pKa = 11.84FFAGFGIDD161 pKa = 4.61LEE163 pKa = 4.75SKK165 pKa = 10.26PRR167 pKa = 11.84TARR170 pKa = 11.84PLCRR174 pKa = 11.84TCLDD178 pKa = 2.96WSEE181 pKa = 4.48RR182 pKa = 11.84RR183 pKa = 11.84MHH185 pKa = 6.83LGGRR189 pKa = 11.84LGASLLARR197 pKa = 11.84SIEE200 pKa = 4.24RR201 pKa = 11.84GWVRR205 pKa = 11.84TIRR208 pKa = 11.84DD209 pKa = 3.26SRR211 pKa = 11.84ALEE214 pKa = 3.79VTRR217 pKa = 11.84AGEE220 pKa = 3.94RR221 pKa = 11.84GFTEE225 pKa = 4.08TFGIASEE232 pKa = 4.09MLRR235 pKa = 11.84PPRR238 pKa = 4.55

Molecular weight:
25.75 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4798

0

4798

1545255

27

3958

322.1

34.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.586 ± 0.044

0.828 ± 0.013

5.882 ± 0.034

5.447 ± 0.034

3.813 ± 0.025

8.923 ± 0.054

2.031 ± 0.021

5.514 ± 0.029

2.914 ± 0.027

9.945 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.707 ± 0.019

2.587 ± 0.023

4.983 ± 0.031

2.863 ± 0.018

6.954 ± 0.048

5.866 ± 0.033

5.349 ± 0.042

7.369 ± 0.029

1.276 ± 0.014

2.165 ± 0.019

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski