Tengunoibacter tsumagoiensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Ktedonobacteria; Ktedonobacterales; Dictyobacteraceae; Tengunoibacter

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5981 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A401ZXI6|A0A401ZXI6_9CHLR Glycine/betaine ABC transporter substrate-binding protein OS=Tengunoibacter tsumagoiensis OX=2014871 GN=KTT_14270 PE=4 SV=1
MM1 pKa = 7.14QFNSITVSDD10 pKa = 4.48VIPVGEE16 pKa = 4.66KK17 pKa = 10.5DD18 pKa = 3.42LNAYY22 pKa = 9.72GSDD25 pKa = 3.16WDD27 pKa = 3.45IHH29 pKa = 6.07FSSTVSPAVITATYY43 pKa = 9.9VGTYY47 pKa = 8.72PVNAGEE53 pKa = 4.55ALPPIIITATLTNDD67 pKa = 3.24AVPSVTDD74 pKa = 3.39VAVVSTPGNLDD85 pKa = 3.42TNNIAIDD92 pKa = 3.78TVFVEE97 pKa = 4.57NNNNNHH103 pKa = 6.16SNSNDD108 pKa = 3.12HH109 pKa = 6.43NQNNQNNNHH118 pKa = 5.66QNNNHH123 pKa = 5.86QNNQNNNNNNNNNNNNNSNNSSPGLPFTGSDD154 pKa = 3.46PDD156 pKa = 3.45QEE158 pKa = 4.43INILAYY164 pKa = 10.59LL165 pKa = 3.99

Molecular weight: 17.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A402A604|A0A402A604_9CHLR DNA mismatch repair protein MutT OS=Tengunoibacter tsumagoiensis OX=2014871 GN=KTT_44090 PE=4 SV=1
MM1 pKa = 7.37ILVLRR6 pKa = 11.84VLRR9 pKa = 11.84VIRR12 pKa = 11.84VTQVLKK18 pKa = 10.71ARR20 pKa = 11.84RR21 pKa = 11.84VLRR24 pKa = 11.84GILVRR29 pKa = 11.84KK30 pKa = 7.63VRR32 pKa = 11.84KK33 pKa = 8.23VRR35 pKa = 11.84KK36 pKa = 7.6VRR38 pKa = 11.84KK39 pKa = 8.5VLRR42 pKa = 11.84VNQEE46 pKa = 3.32

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5981

0

5981

2099093

39

10907

351.0

38.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.05 ± 0.03

0.955 ± 0.011

4.629 ± 0.02

5.892 ± 0.042

3.761 ± 0.02

6.955 ± 0.032

2.611 ± 0.017

5.999 ± 0.025

3.274 ± 0.03

11.428 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.097 ± 0.013

3.275 ± 0.031

5.104 ± 0.03

5.505 ± 0.037

5.692 ± 0.033

6.436 ± 0.031

6.139 ± 0.04

6.556 ± 0.026

1.491 ± 0.017

3.149 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski