Aeromonas phage CF8
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 244 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y5TLU2|A0A4Y5TLU2_9CAUD Uncharacterized protein OS=Aeromonas phage CF8 OX=2575324 GN=CF8_0120 PE=4 SV=1
MM1 pKa = 7.87 GINTDD6 pKa = 2.84 IVVYY10 pKa = 10.01 KK11 pKa = 10.3 FNSIDD16 pKa = 3.6 LEE18 pKa = 4.64 DD19 pKa = 4.63 YY20 pKa = 11.01 VEE22 pKa = 4.5 GWNEE26 pKa = 3.85 NQITLALEE34 pKa = 4.29 GALNLIFHH42 pKa = 7.12 PSVTSNTEE50 pKa = 3.39 IYY52 pKa = 10.58 AGEE55 pKa = 4.01 PWSEE59 pKa = 3.91 IEE61 pKa = 4.05 NQIVTEE67 pKa = 4.19 TVNLIQSHH75 pKa = 6.1 SDD77 pKa = 2.82 GSEE80 pKa = 3.5 FGFRR84 pKa = 11.84 LFDD87 pKa = 3.57 TEE89 pKa = 4.23 EE90 pKa = 4.1 EE91 pKa = 4.17 EE92 pKa = 4.83 EE93 pKa = 4.21 RR94 pKa = 11.84 WDD96 pKa = 5.03 LLFLEE101 pKa = 4.35 QTNNIEE107 pKa = 4.11 AQFGSLLEE115 pKa = 4.35 KK116 pKa = 10.38 LAEE119 pKa = 5.32 DD120 pKa = 4.25 ISSDD124 pKa = 3.72 HH125 pKa = 6.54 YY126 pKa = 11.7 NPDD129 pKa = 2.91 VNTLYY134 pKa = 11.46 GEE136 pKa = 4.31 MMLSAKK142 pKa = 10.43 LILRR146 pKa = 11.84 SYY148 pKa = 11.08 AYY150 pKa = 9.34 YY151 pKa = 10.36 TDD153 pKa = 3.54 TGVLRR158 pKa = 11.84 LAIQCQQ164 pKa = 3.23
Molecular weight: 18.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.986
IPC_protein 3.91
Toseland 3.745
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.745
Rodwell 3.745
Grimsley 3.656
Solomon 3.859
Lehninger 3.808
Nozaki 3.973
DTASelect 4.101
Thurlkill 3.757
EMBOSS 3.757
Sillero 4.024
Patrickios 1.036
IPC_peptide 3.859
IPC2_peptide 4.012
IPC2.peptide.svr19 3.922
Protein with the highest isoelectric point:
>tr|A0A4Y5TP62|A0A4Y5TP62_9CAUD Uncharacterized protein OS=Aeromonas phage CF8 OX=2575324 GN=CF8_0212 PE=4 SV=1
MM1 pKa = 7.68 FKK3 pKa = 10.76 NLWFWLRR10 pKa = 11.84 VRR12 pKa = 11.84 WLIVYY17 pKa = 8.76 FNLWQFKK24 pKa = 9.96 EE25 pKa = 4.2 RR26 pKa = 11.84 VLNACFCPKK35 pKa = 9.89 RR36 pKa = 11.84 NHH38 pKa = 6.16 VLTQGHH44 pKa = 7.32 IINLRR49 pKa = 11.84 MGNDD53 pKa = 3.48 VILIRR58 pKa = 11.84 HH59 pKa = 4.93 CTWWFGRR66 pKa = 11.84 QGFKK70 pKa = 10.94 LIGVKK75 pKa = 10.53 YY76 pKa = 9.01 PVNSGIRR83 pKa = 11.84 LVGQDD88 pKa = 2.72 VCLRR92 pKa = 11.84 LKK94 pKa = 10.66 PNEE97 pKa = 4.12 TASQAVIRR105 pKa = 11.84 YY106 pKa = 6.76 NATVRR111 pKa = 11.84 KK112 pKa = 9.2 VHH114 pKa = 7.07 DD115 pKa = 3.89 AAEE118 pKa = 4.28 QINQITKK125 pKa = 9.6 EE126 pKa = 4.25 LNSKK130 pKa = 10.59 SPFIKK135 pKa = 10.53 AA136 pKa = 3.28
Molecular weight: 16.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.307
IPC2_protein 9.604
IPC_protein 9.897
Toseland 10.599
ProMoST 10.16
Dawson 10.701
Bjellqvist 10.335
Wikipedia 10.833
Rodwell 11.125
Grimsley 10.73
Solomon 10.76
Lehninger 10.73
Nozaki 10.599
DTASelect 10.321
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.613
Patrickios 10.862
IPC_peptide 10.76
IPC2_peptide 9.341
IPC2.peptide.svr19 8.519
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
244
0
244
73531
39
2354
301.4
34.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.86 ± 0.184
0.998 ± 0.06
6.214 ± 0.083
6.512 ± 0.149
4.769 ± 0.08
6.567 ± 0.17
1.972 ± 0.075
6.54 ± 0.112
6.56 ± 0.155
8.939 ± 0.143
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.645 ± 0.064
5.636 ± 0.143
3.985 ± 0.097
3.88 ± 0.093
4.455 ± 0.092
6.299 ± 0.111
6.019 ± 0.127
6.925 ± 0.14
1.171 ± 0.055
4.053 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here