Capybara microvirus Cap3_SP_344
Average proteome isoelectric point is 5.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W8E2|A0A4P8W8E2_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_344 OX=2585432 PE=4 SV=1
MM1 pKa = 7.22 HH2 pKa = 7.18 NCWPSTPCGLVALTGDD18 pKa = 3.17 ITQCEE23 pKa = 4.48 DD24 pKa = 4.27 CPDD27 pKa = 3.24 IDD29 pKa = 5.37 ARR31 pKa = 11.84 FADD34 pKa = 4.07 SFFEE38 pKa = 3.92 EE39 pKa = 4.58 TPRR42 pKa = 11.84 YY43 pKa = 9.87 DD44 pKa = 3.66 EE45 pKa = 4.97 RR46 pKa = 11.84 CALMNDD52 pKa = 3.03 ICSFSYY58 pKa = 10.8 VDD60 pKa = 3.76 EE61 pKa = 4.97 NGDD64 pKa = 3.37 YY65 pKa = 11.07 DD66 pKa = 5.11 LPFF69 pKa = 5.49
Molecular weight: 7.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.892
IPC2_protein 3.656
IPC_protein 3.605
Toseland 3.389
ProMoST 3.808
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.592
Rodwell 3.439
Grimsley 3.312
Solomon 3.579
Lehninger 3.541
Nozaki 3.757
DTASelect 3.973
Thurlkill 3.478
EMBOSS 3.592
Sillero 3.732
Patrickios 0.006
IPC_peptide 3.579
IPC2_peptide 3.694
IPC2.peptide.svr19 3.7
Protein with the highest isoelectric point:
>tr|A0A4P8W4T0|A0A4P8W4T0_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_344 OX=2585432 PE=4 SV=1
MM1 pKa = 7.65 CLFPKK6 pKa = 10.49 RR7 pKa = 11.84 IRR9 pKa = 11.84 NPKK12 pKa = 8.67 KK13 pKa = 10.26 DD14 pKa = 3.53 FRR16 pKa = 11.84 SGYY19 pKa = 9.65 DD20 pKa = 3.74 PEE22 pKa = 4.59 WLTVPCGNCYY32 pKa = 10.86 DD33 pKa = 5.17 CIQKK37 pKa = 10.59 NIRR40 pKa = 11.84 DD41 pKa = 3.31 WSARR45 pKa = 11.84 ARR47 pKa = 11.84 AEE49 pKa = 3.73 YY50 pKa = 10.24 HH51 pKa = 6.95 DD52 pKa = 4.27 YY53 pKa = 10.86 KK54 pKa = 11.25 EE55 pKa = 4.46 KK56 pKa = 10.73 GGCVLFPTLTYY67 pKa = 10.72 NEE69 pKa = 4.45 KK70 pKa = 10.7 CCPCVNIGNKK80 pKa = 8.76 SYY82 pKa = 10.98 RR83 pKa = 11.84 AFSKK87 pKa = 10.6 RR88 pKa = 11.84 DD89 pKa = 3.24 VQHH92 pKa = 6.7 FIKK95 pKa = 10.54 KK96 pKa = 8.82 FRR98 pKa = 11.84 VYY100 pKa = 10.65 VSRR103 pKa = 11.84 TYY105 pKa = 10.96 GKK107 pKa = 10.24 EE108 pKa = 3.36 FARR111 pKa = 11.84 GIKK114 pKa = 10.43 YY115 pKa = 9.45 MIASEE120 pKa = 4.52 FGTSSGKK127 pKa = 6.23 THH129 pKa = 6.94 RR130 pKa = 11.84 PHH132 pKa = 5.38 YY133 pKa = 10.21 HH134 pKa = 6.75 CLLFLPWRR142 pKa = 11.84 PKK144 pKa = 9.39 IRR146 pKa = 11.84 KK147 pKa = 8.01 ISSIIRR153 pKa = 11.84 KK154 pKa = 7.33 SWRR157 pKa = 11.84 FGYY160 pKa = 9.63 MSWSKK165 pKa = 11.15 KK166 pKa = 9.35 HH167 pKa = 5.37 GAVINSVKK175 pKa = 10.49 GISYY179 pKa = 9.0 VCKK182 pKa = 10.64 YY183 pKa = 8.65 MCKK186 pKa = 9.8 QSNWYY191 pKa = 9.51 HH192 pKa = 4.87 FTGDD196 pKa = 3.44 VPRR199 pKa = 11.84 YY200 pKa = 9.06 IYY202 pKa = 10.82 KK203 pKa = 10.64 GDD205 pKa = 3.86 DD206 pKa = 3.45 RR207 pKa = 11.84 YY208 pKa = 11.39 CNPDD212 pKa = 3.21 FEE214 pKa = 5.16 KK215 pKa = 10.78 LSKK218 pKa = 10.72 VKK220 pKa = 10.1 PFHH223 pKa = 6.03 LQSIGFGLCLNKK235 pKa = 10.41 YY236 pKa = 9.68 VDD238 pKa = 3.46 TSEE241 pKa = 5.34 IIDD244 pKa = 3.78 FRR246 pKa = 11.84 FHH248 pKa = 6.58 FPKK251 pKa = 10.7 DD252 pKa = 3.25 SAVPDD257 pKa = 4.01 DD258 pKa = 3.63 SCYY261 pKa = 9.94 YY262 pKa = 9.88 TIPQYY267 pKa = 10.98 NLHH270 pKa = 6.38 YY271 pKa = 10.34 LIQDD275 pKa = 4.05 KK276 pKa = 10.83 IDD278 pKa = 4.13 GEE280 pKa = 3.99 WSYY283 pKa = 12.13 KK284 pKa = 9.95 NKK286 pKa = 10.21 TYY288 pKa = 11.47 DD289 pKa = 3.28 FMQKK293 pKa = 9.66 VFDD296 pKa = 4.46 FKK298 pKa = 11.4 VRR300 pKa = 11.84 CNWYY304 pKa = 9.85 HH305 pKa = 7.19 LACQLDD311 pKa = 3.88 KK312 pKa = 11.51 DD313 pKa = 4.59 LLNSCGISEE322 pKa = 4.35 FNKK325 pKa = 10.71 KK326 pKa = 9.04 IDD328 pKa = 3.89 NIFDD332 pKa = 3.79 EE333 pKa = 4.56 TSLNYY338 pKa = 10.11 RR339 pKa = 11.84 EE340 pKa = 4.46 IANFMVSYY348 pKa = 10.56 FGTNYY353 pKa = 9.54 FPPIDD358 pKa = 4.61 FYY360 pKa = 11.79 CDD362 pKa = 3.18 LGSSFRR368 pKa = 11.84 HH369 pKa = 5.21 WYY371 pKa = 8.29 MRR373 pKa = 11.84 YY374 pKa = 9.95 LDD376 pKa = 4.41 FDD378 pKa = 4.42 SDD380 pKa = 3.69 SRR382 pKa = 11.84 RR383 pKa = 11.84 AKK385 pKa = 10.11 SDD387 pKa = 3.36 CVTYY391 pKa = 9.88 EE392 pKa = 4.01 YY393 pKa = 11.18 KK394 pKa = 10.78 KK395 pKa = 9.48 NTPLCYY401 pKa = 10.26 VSLLLDD407 pKa = 4.06 SIRR410 pKa = 11.84 LSVEE414 pKa = 3.78 NYY416 pKa = 10.15 VKK418 pKa = 10.2 GTTDD422 pKa = 3.77 LKK424 pKa = 9.72 RR425 pKa = 11.84 HH426 pKa = 5.61 KK427 pKa = 9.9 FASRR431 pKa = 11.84 KK432 pKa = 9.58 KK433 pKa = 10.18 VIDD436 pKa = 3.59 YY437 pKa = 10.76 NSNFSS442 pKa = 3.4
Molecular weight: 52.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.654
IPC2_protein 8.58
IPC_protein 8.404
Toseland 8.799
ProMoST 8.916
Dawson 9.268
Bjellqvist 9.253
Wikipedia 9.414
Rodwell 9.385
Grimsley 9.238
Solomon 9.297
Lehninger 9.253
Nozaki 9.355
DTASelect 9.107
Thurlkill 9.18
EMBOSS 9.385
Sillero 9.385
Patrickios 3.948
IPC_peptide 9.282
IPC2_peptide 8.141
IPC2.peptide.svr19 7.809
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1693
67
653
282.2
31.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.675 ± 1.868
2.185 ± 0.966
5.848 ± 0.963
5.08 ± 1.131
5.552 ± 0.942
6.084 ± 1.001
1.89 ± 0.428
4.607 ± 0.487
6.261 ± 1.152
8.328 ± 1.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.067 ± 0.324
5.611 ± 0.308
4.017 ± 0.375
3.721 ± 0.722
4.371 ± 0.671
9.569 ± 0.644
4.903 ± 0.834
6.143 ± 0.585
1.24 ± 0.431
5.848 ± 1.252
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here