Amino acid dipepetide frequency for Lelliottia phage phD2B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.242AlaAla: 10.242 ± 1.5
0.737AlaCys: 0.737 ± 0.229
5.894AlaAsp: 5.894 ± 0.957
7.294AlaGlu: 7.294 ± 0.96
3.684AlaPhe: 3.684 ± 0.579
7.884AlaGly: 7.884 ± 0.959
1.842AlaHis: 1.842 ± 0.386
5.526AlaIle: 5.526 ± 0.705
5.305AlaLys: 5.305 ± 0.799
6.558AlaLeu: 6.558 ± 0.822
3.684AlaMet: 3.684 ± 0.581
3.463AlaAsn: 3.463 ± 0.483
3.095AlaPro: 3.095 ± 0.418
4.568AlaGln: 4.568 ± 0.571
4.789AlaArg: 4.789 ± 0.691
5.673AlaSer: 5.673 ± 0.685
4.568AlaThr: 4.568 ± 0.855
6.852AlaVal: 6.852 ± 0.748
1.032AlaTrp: 1.032 ± 0.342
3.095AlaTyr: 3.095 ± 0.514
0.0AlaXaa: 0.0 ± 0.0
Cys
0.442CysAla: 0.442 ± 0.158
0.0CysCys: 0.0 ± 0.0
0.516CysAsp: 0.516 ± 0.237
0.516CysGlu: 0.516 ± 0.187
0.368CysPhe: 0.368 ± 0.151
0.884CysGly: 0.884 ± 0.292
0.221CysHis: 0.221 ± 0.137
0.589CysIle: 0.589 ± 0.264
0.81CysLys: 0.81 ± 0.272
0.958CysLeu: 0.958 ± 0.233
0.295CysMet: 0.295 ± 0.138
0.516CysAsn: 0.516 ± 0.219
0.516CysPro: 0.516 ± 0.171
0.368CysGln: 0.368 ± 0.153
0.589CysArg: 0.589 ± 0.228
0.516CysSer: 0.516 ± 0.183
0.295CysThr: 0.295 ± 0.147
0.958CysVal: 0.958 ± 0.268
0.074CysTrp: 0.074 ± 0.07
0.663CysTyr: 0.663 ± 0.245
0.0CysXaa: 0.0 ± 0.0
Asp
7.147AspAla: 7.147 ± 0.487
0.737AspCys: 0.737 ± 0.22
2.947AspAsp: 2.947 ± 0.431
4.052AspGlu: 4.052 ± 0.555
1.842AspPhe: 1.842 ± 0.332
4.274AspGly: 4.274 ± 0.687
1.326AspHis: 1.326 ± 0.396
3.831AspIle: 3.831 ± 0.561
4.642AspLys: 4.642 ± 0.524
4.789AspLeu: 4.789 ± 0.591
2.063AspMet: 2.063 ± 0.496
1.989AspAsn: 1.989 ± 0.525
1.989AspPro: 1.989 ± 0.289
1.179AspGln: 1.179 ± 0.284
2.505AspArg: 2.505 ± 0.356
2.874AspSer: 2.874 ± 0.469
3.758AspThr: 3.758 ± 0.541
3.831AspVal: 3.831 ± 0.408
0.884AspTrp: 0.884 ± 0.2
1.621AspTyr: 1.621 ± 0.422
0.0AspXaa: 0.0 ± 0.0
Glu
7.294GluAla: 7.294 ± 0.937
0.884GluCys: 0.884 ± 0.253
4.126GluAsp: 4.126 ± 0.44
4.421GluGlu: 4.421 ± 0.702
3.095GluPhe: 3.095 ± 0.351
5.305GluGly: 5.305 ± 0.477
1.695GluHis: 1.695 ± 0.354
2.726GluIle: 2.726 ± 0.428
3.758GluLys: 3.758 ± 0.64
5.673GluLeu: 5.673 ± 0.748
2.653GluMet: 2.653 ± 0.375
1.989GluAsn: 1.989 ± 0.367
2.137GluPro: 2.137 ± 0.378
2.8GluGln: 2.8 ± 0.455
2.947GluArg: 2.947 ± 0.526
3.389GluSer: 3.389 ± 0.372
3.242GluThr: 3.242 ± 0.384
5.158GluVal: 5.158 ± 0.581
1.326GluTrp: 1.326 ± 0.379
1.4GluTyr: 1.4 ± 0.335
0.0GluXaa: 0.0 ± 0.0
Phe
3.242PheAla: 3.242 ± 0.488
0.516PheCys: 0.516 ± 0.185
3.021PheAsp: 3.021 ± 0.396
2.653PheGlu: 2.653 ± 0.301
1.842PhePhe: 1.842 ± 0.409
2.8PheGly: 2.8 ± 0.477
0.368PheHis: 0.368 ± 0.13
2.358PheIle: 2.358 ± 0.373
2.653PheLys: 2.653 ± 0.349
3.021PheLeu: 3.021 ± 0.51
1.179PheMet: 1.179 ± 0.285
2.579PheAsn: 2.579 ± 0.36
1.474PhePro: 1.474 ± 0.336
0.958PheGln: 0.958 ± 0.243
2.358PheArg: 2.358 ± 0.586
2.284PheSer: 2.284 ± 0.544
1.768PheThr: 1.768 ± 0.314
1.916PheVal: 1.916 ± 0.401
0.295PheTrp: 0.295 ± 0.139
1.253PheTyr: 1.253 ± 0.318
0.0PheXaa: 0.0 ± 0.0
Gly
7.958GlyAla: 7.958 ± 0.83
0.81GlyCys: 0.81 ± 0.286
5.894GlyAsp: 5.894 ± 0.758
4.937GlyGlu: 4.937 ± 0.638
3.463GlyPhe: 3.463 ± 0.478
7.294GlyGly: 7.294 ± 1.043
2.137GlyHis: 2.137 ± 0.558
4.789GlyIle: 4.789 ± 0.673
7.663GlyLys: 7.663 ± 0.925
5.379GlyLeu: 5.379 ± 0.779
1.547GlyMet: 1.547 ± 0.25
3.905GlyAsn: 3.905 ± 0.761
0.516GlyPro: 0.516 ± 0.215
2.726GlyGln: 2.726 ± 0.336
4.495GlyArg: 4.495 ± 0.544
5.452GlySer: 5.452 ± 0.652
4.937GlyThr: 4.937 ± 0.646
5.452GlyVal: 5.452 ± 0.708
1.474GlyTrp: 1.474 ± 0.254
3.242GlyTyr: 3.242 ± 0.34
0.0GlyXaa: 0.0 ± 0.0
His
1.916HisAla: 1.916 ± 0.456
0.074HisCys: 0.074 ± 0.067
1.474HisAsp: 1.474 ± 0.343
0.884HisGlu: 0.884 ± 0.227
1.253HisPhe: 1.253 ± 0.254
1.768HisGly: 1.768 ± 0.523
0.663HisHis: 0.663 ± 0.292
1.179HisIle: 1.179 ± 0.289
1.474HisLys: 1.474 ± 0.455
2.21HisLeu: 2.21 ± 0.372
1.105HisMet: 1.105 ± 0.378
1.105HisAsn: 1.105 ± 0.311
0.589HisPro: 0.589 ± 0.193
0.737HisGln: 0.737 ± 0.256
1.621HisArg: 1.621 ± 0.384
0.958HisSer: 0.958 ± 0.313
1.032HisThr: 1.032 ± 0.31
1.253HisVal: 1.253 ± 0.299
0.295HisTrp: 0.295 ± 0.243
0.958HisTyr: 0.958 ± 0.2
0.0HisXaa: 0.0 ± 0.0
Ile
4.2IleAla: 4.2 ± 0.553
0.221IleCys: 0.221 ± 0.128
3.61IleAsp: 3.61 ± 0.394
3.831IleGlu: 3.831 ± 0.55
1.4IlePhe: 1.4 ± 0.363
4.495IleGly: 4.495 ± 0.638
0.884IleHis: 0.884 ± 0.215
2.431IleIle: 2.431 ± 0.584
3.095IleLys: 3.095 ± 0.407
2.947IleLeu: 2.947 ± 0.367
1.105IleMet: 1.105 ± 0.353
2.505IleAsn: 2.505 ± 0.445
1.989IlePro: 1.989 ± 0.36
1.768IleGln: 1.768 ± 0.358
2.726IleArg: 2.726 ± 0.404
2.947IleSer: 2.947 ± 0.488
3.905IleThr: 3.905 ± 0.556
4.347IleVal: 4.347 ± 0.495
0.884IleTrp: 0.884 ± 0.271
1.4IleTyr: 1.4 ± 0.398
0.0IleXaa: 0.0 ± 0.0
Lys
6.41LysAla: 6.41 ± 0.871
1.105LysCys: 1.105 ± 0.301
2.874LysAsp: 2.874 ± 0.478
4.937LysGlu: 4.937 ± 0.734
1.842LysPhe: 1.842 ± 0.387
6.042LysGly: 6.042 ± 0.671
1.842LysHis: 1.842 ± 0.373
2.505LysIle: 2.505 ± 0.42
3.389LysLys: 3.389 ± 0.638
5.01LysLeu: 5.01 ± 0.571
2.284LysMet: 2.284 ± 0.437
1.621LysAsn: 1.621 ± 0.423
2.874LysPro: 2.874 ± 0.387
2.21LysGln: 2.21 ± 0.465
3.463LysArg: 3.463 ± 0.43
3.61LysSer: 3.61 ± 0.446
2.874LysThr: 2.874 ± 0.436
4.789LysVal: 4.789 ± 0.554
0.737LysTrp: 0.737 ± 0.182
1.916LysTyr: 1.916 ± 0.383
0.0LysXaa: 0.0 ± 0.0
Leu
5.747LeuAla: 5.747 ± 0.796
0.589LeuCys: 0.589 ± 0.174
5.452LeuAsp: 5.452 ± 0.552
4.642LeuGlu: 4.642 ± 0.668
2.431LeuPhe: 2.431 ± 0.502
5.305LeuGly: 5.305 ± 0.594
1.621LeuHis: 1.621 ± 0.366
3.242LeuIle: 3.242 ± 0.504
4.568LeuLys: 4.568 ± 0.55
5.01LeuLeu: 5.01 ± 0.567
2.063LeuMet: 2.063 ± 0.482
3.61LeuAsn: 3.61 ± 0.414
3.537LeuPro: 3.537 ± 0.456
3.61LeuGln: 3.61 ± 0.695
5.084LeuArg: 5.084 ± 0.61
5.158LeuSer: 5.158 ± 0.693
4.568LeuThr: 4.568 ± 0.605
4.789LeuVal: 4.789 ± 0.479
0.958LeuTrp: 0.958 ± 0.279
1.916LeuTyr: 1.916 ± 0.394
0.0LeuXaa: 0.0 ± 0.0
Met
3.61MetAla: 3.61 ± 0.478
0.221MetCys: 0.221 ± 0.13
1.474MetAsp: 1.474 ± 0.338
1.621MetGlu: 1.621 ± 0.299
1.179MetPhe: 1.179 ± 0.244
2.284MetGly: 2.284 ± 0.573
0.442MetHis: 0.442 ± 0.171
1.695MetIle: 1.695 ± 0.297
1.768MetLys: 1.768 ± 0.371
2.358MetLeu: 2.358 ± 0.441
1.4MetMet: 1.4 ± 0.265
1.4MetAsn: 1.4 ± 0.298
1.253MetPro: 1.253 ± 0.294
1.695MetGln: 1.695 ± 0.488
1.916MetArg: 1.916 ± 0.44
2.726MetSer: 2.726 ± 0.404
1.916MetThr: 1.916 ± 0.355
2.653MetVal: 2.653 ± 0.463
0.295MetTrp: 0.295 ± 0.162
0.589MetTyr: 0.589 ± 0.249
0.0MetXaa: 0.0 ± 0.0
Asn
4.2AsnAla: 4.2 ± 0.642
0.516AsnCys: 0.516 ± 0.211
1.768AsnAsp: 1.768 ± 0.341
2.505AsnGlu: 2.505 ± 0.528
1.695AsnPhe: 1.695 ± 0.335
3.463AsnGly: 3.463 ± 0.407
0.884AsnHis: 0.884 ± 0.199
1.768AsnIle: 1.768 ± 0.443
3.389AsnLys: 3.389 ± 0.359
3.463AsnLeu: 3.463 ± 0.452
1.253AsnMet: 1.253 ± 0.293
1.695AsnAsn: 1.695 ± 0.392
1.989AsnPro: 1.989 ± 0.331
1.989AsnGln: 1.989 ± 0.499
2.8AsnArg: 2.8 ± 0.494
1.842AsnSer: 1.842 ± 0.363
3.242AsnThr: 3.242 ± 0.462
3.61AsnVal: 3.61 ± 0.749
0.516AsnTrp: 0.516 ± 0.18
1.621AsnTyr: 1.621 ± 0.315
0.0AsnXaa: 0.0 ± 0.0
Pro
3.684ProAla: 3.684 ± 0.484
1.032ProCys: 1.032 ± 0.307
2.137ProAsp: 2.137 ± 0.365
3.537ProGlu: 3.537 ± 0.442
1.326ProPhe: 1.326 ± 0.246
1.989ProGly: 1.989 ± 0.508
0.81ProHis: 0.81 ± 0.285
1.032ProIle: 1.032 ± 0.266
1.989ProLys: 1.989 ± 0.364
2.653ProLeu: 2.653 ± 0.415
0.958ProMet: 0.958 ± 0.242
1.4ProAsn: 1.4 ± 0.322
0.884ProPro: 0.884 ± 0.239
1.547ProGln: 1.547 ± 0.225
1.4ProArg: 1.4 ± 0.278
1.916ProSer: 1.916 ± 0.363
2.358ProThr: 2.358 ± 0.659
2.358ProVal: 2.358 ± 0.354
0.368ProTrp: 0.368 ± 0.159
1.4ProTyr: 1.4 ± 0.285
0.0ProXaa: 0.0 ± 0.0
Gln
4.421GlnAla: 4.421 ± 0.704
0.295GlnCys: 0.295 ± 0.14
1.916GlnAsp: 1.916 ± 0.37
2.431GlnGlu: 2.431 ± 0.518
2.284GlnPhe: 2.284 ± 0.371
3.316GlnGly: 3.316 ± 0.584
0.884GlnHis: 0.884 ± 0.268
2.137GlnIle: 2.137 ± 0.354
1.253GlnLys: 1.253 ± 0.301
2.726GlnLeu: 2.726 ± 0.406
1.4GlnMet: 1.4 ± 0.332
1.547GlnAsn: 1.547 ± 0.343
1.032GlnPro: 1.032 ± 0.261
2.21GlnGln: 2.21 ± 0.453
2.505GlnArg: 2.505 ± 0.54
2.505GlnSer: 2.505 ± 0.442
1.989GlnThr: 1.989 ± 0.417
2.947GlnVal: 2.947 ± 0.423
0.368GlnTrp: 0.368 ± 0.188
1.695GlnTyr: 1.695 ± 0.319
0.0GlnXaa: 0.0 ± 0.0
Arg
4.274ArgAla: 4.274 ± 0.741
0.442ArgCys: 0.442 ± 0.2
2.947ArgAsp: 2.947 ± 0.502
4.052ArgGlu: 4.052 ± 0.707
1.4ArgPhe: 1.4 ± 0.344
4.937ArgGly: 4.937 ± 0.63
1.4ArgHis: 1.4 ± 0.335
3.463ArgIle: 3.463 ± 0.602
2.726ArgLys: 2.726 ± 0.379
4.863ArgLeu: 4.863 ± 0.571
2.358ArgMet: 2.358 ± 0.425
2.8ArgAsn: 2.8 ± 0.488
1.768ArgPro: 1.768 ± 0.36
2.284ArgGln: 2.284 ± 0.364
3.242ArgArg: 3.242 ± 0.411
2.579ArgSer: 2.579 ± 0.316
3.316ArgThr: 3.316 ± 0.505
4.052ArgVal: 4.052 ± 0.429
0.737ArgTrp: 0.737 ± 0.221
1.695ArgTyr: 1.695 ± 0.338
0.0ArgXaa: 0.0 ± 0.0
Ser
4.716SerAla: 4.716 ± 0.487
0.442SerCys: 0.442 ± 0.163
2.284SerAsp: 2.284 ± 0.339
4.421SerGlu: 4.421 ± 0.673
1.916SerPhe: 1.916 ± 0.295
5.894SerGly: 5.894 ± 0.762
1.547SerHis: 1.547 ± 0.339
3.242SerIle: 3.242 ± 0.403
2.653SerLys: 2.653 ± 0.46
4.568SerLeu: 4.568 ± 0.604
2.063SerMet: 2.063 ± 0.465
3.242SerAsn: 3.242 ± 0.347
2.137SerPro: 2.137 ± 0.527
2.431SerGln: 2.431 ± 0.377
3.537SerArg: 3.537 ± 0.508
3.831SerSer: 3.831 ± 0.53
3.905SerThr: 3.905 ± 0.599
3.979SerVal: 3.979 ± 0.537
0.81SerTrp: 0.81 ± 0.173
1.695SerTyr: 1.695 ± 0.339
0.0SerXaa: 0.0 ± 0.0
Thr
5.158ThrAla: 5.158 ± 0.738
0.663ThrCys: 0.663 ± 0.203
3.684ThrAsp: 3.684 ± 0.753
2.874ThrGlu: 2.874 ± 0.477
3.463ThrPhe: 3.463 ± 0.624
6.042ThrGly: 6.042 ± 0.769
1.474ThrHis: 1.474 ± 0.393
2.874ThrIle: 2.874 ± 0.538
3.537ThrLys: 3.537 ± 0.591
4.421ThrLeu: 4.421 ± 0.577
1.253ThrMet: 1.253 ± 0.276
2.063ThrAsn: 2.063 ± 0.448
1.695ThrPro: 1.695 ± 0.309
2.358ThrGln: 2.358 ± 0.407
3.095ThrArg: 3.095 ± 0.361
3.537ThrSer: 3.537 ± 0.627
4.274ThrThr: 4.274 ± 0.931
3.831ThrVal: 3.831 ± 0.652
0.589ThrTrp: 0.589 ± 0.171
1.474ThrTyr: 1.474 ± 0.338
0.0ThrXaa: 0.0 ± 0.0
Val
6.705ValAla: 6.705 ± 0.762
0.442ValCys: 0.442 ± 0.166
3.831ValAsp: 3.831 ± 0.541
3.979ValGlu: 3.979 ± 0.548
2.579ValPhe: 2.579 ± 0.451
6.41ValGly: 6.41 ± 0.784
1.547ValHis: 1.547 ± 0.381
3.242ValIle: 3.242 ± 0.445
4.495ValLys: 4.495 ± 0.545
4.274ValLeu: 4.274 ± 0.454
2.431ValMet: 2.431 ± 0.447
3.979ValAsn: 3.979 ± 0.525
3.389ValPro: 3.389 ± 0.469
2.874ValGln: 2.874 ± 0.608
4.495ValArg: 4.495 ± 0.663
4.863ValSer: 4.863 ± 0.565
3.684ValThr: 3.684 ± 0.572
5.158ValVal: 5.158 ± 0.644
0.737ValTrp: 0.737 ± 0.234
1.621ValTyr: 1.621 ± 0.455
0.0ValXaa: 0.0 ± 0.0
Trp
1.253TrpAla: 1.253 ± 0.307
0.074TrpCys: 0.074 ± 0.075
0.589TrpAsp: 0.589 ± 0.247
1.4TrpGlu: 1.4 ± 0.272
0.295TrpPhe: 0.295 ± 0.173
0.884TrpGly: 0.884 ± 0.217
0.221TrpHis: 0.221 ± 0.127
0.442TrpIle: 0.442 ± 0.153
1.326TrpLys: 1.326 ± 0.401
0.737TrpLeu: 0.737 ± 0.259
0.295TrpMet: 0.295 ± 0.131
0.884TrpAsn: 0.884 ± 0.242
0.958TrpPro: 0.958 ± 0.29
0.516TrpGln: 0.516 ± 0.198
0.368TrpArg: 0.368 ± 0.148
0.81TrpSer: 0.81 ± 0.271
0.442TrpThr: 0.442 ± 0.196
1.179TrpVal: 1.179 ± 0.376
0.295TrpTrp: 0.295 ± 0.125
0.074TrpTyr: 0.074 ± 0.075
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.168TyrAla: 3.168 ± 0.578
0.295TyrCys: 0.295 ± 0.133
1.842TyrAsp: 1.842 ± 0.261
1.032TyrGlu: 1.032 ± 0.235
1.179TyrPhe: 1.179 ± 0.25
2.8TyrGly: 2.8 ± 0.421
0.737TyrHis: 0.737 ± 0.26
1.621TyrIle: 1.621 ± 0.221
1.842TyrLys: 1.842 ± 0.351
2.358TyrLeu: 2.358 ± 0.317
1.032TyrMet: 1.032 ± 0.243
1.916TyrAsn: 1.916 ± 0.344
0.958TyrPro: 0.958 ± 0.267
1.032TyrGln: 1.032 ± 0.255
1.4TyrArg: 1.4 ± 0.323
1.842TyrSer: 1.842 ± 0.306
2.284TyrThr: 2.284 ± 0.422
1.621TyrVal: 1.621 ± 0.312
0.368TyrTrp: 0.368 ± 0.181
0.295TyrTyr: 0.295 ± 0.166
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (13573 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski