Leuconostoc phage LDG
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A219VG52|A0A219VG52_9CAUD Lysin OS=Leuconostoc phage LDG OX=1897539 PE=4 SV=1
MM1 pKa = 7.0 TTFDD5 pKa = 3.51 YY6 pKa = 10.99 QKK8 pKa = 10.76 YY9 pKa = 10.68 LNVDD13 pKa = 3.38 DD14 pKa = 5.04 DD15 pKa = 4.92 TYY17 pKa = 11.61 SDD19 pKa = 3.04 WSGTIEE25 pKa = 4.08 EE26 pKa = 5.23 LEE28 pKa = 3.87 NSAINRR34 pKa = 11.84 AQRR37 pKa = 11.84 LNPTITIDD45 pKa = 3.54 TTNKK49 pKa = 10.17 NLYY52 pKa = 9.14 EE53 pKa = 4.06 YY54 pKa = 9.58 AKK56 pKa = 9.37 EE57 pKa = 3.94 VIYY60 pKa = 10.55 IGIFGDD66 pKa = 3.89 ALTPALWQYY75 pKa = 11.21 HH76 pKa = 5.65 DD77 pKa = 4.64 SKK79 pKa = 11.05 MSDD82 pKa = 2.97 LEE84 pKa = 4.45 EE85 pKa = 4.19 YY86 pKa = 9.86 FRR88 pKa = 11.84 WWQEE92 pKa = 3.34
Molecular weight: 11.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.877
IPC2_protein 4.164
IPC_protein 4.075
Toseland 3.884
ProMoST 4.164
Dawson 4.05
Bjellqvist 4.266
Wikipedia 3.961
Rodwell 3.91
Grimsley 3.795
Solomon 4.037
Lehninger 3.986
Nozaki 4.164
DTASelect 4.355
Thurlkill 3.923
EMBOSS 3.973
Sillero 4.19
Patrickios 1.952
IPC_peptide 4.037
IPC2_peptide 4.164
IPC2.peptide.svr19 4.111
Protein with the highest isoelectric point:
>tr|A0A219VG66|A0A219VG66_9CAUD Uncharacterized protein OS=Leuconostoc phage LDG OX=1897539 PE=4 SV=1
MM1 pKa = 7.22 SKK3 pKa = 9.98 VRR5 pKa = 11.84 RR6 pKa = 11.84 EE7 pKa = 3.62 RR8 pKa = 11.84 HH9 pKa = 5.33 KK10 pKa = 11.31 NLVFWRR16 pKa = 11.84 QIGKK20 pKa = 7.63 TLNKK24 pKa = 9.95 VEE26 pKa = 4.2 THH28 pKa = 5.9 HH29 pKa = 6.66 NKK31 pKa = 9.34 PRR33 pKa = 11.84 WRR35 pKa = 11.84 QWEE38 pKa = 3.91 KK39 pKa = 11.38 AEE41 pKa = 3.97 YY42 pKa = 10.29 EE43 pKa = 4.3 RR44 pKa = 11.84 IFKK47 pKa = 10.4 KK48 pKa = 10.77
Molecular weight: 6.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.273
IPC2_protein 9.926
IPC_protein 10.847
Toseland 11.272
ProMoST 11.242
Dawson 11.301
Bjellqvist 11.067
Wikipedia 11.564
Rodwell 11.564
Grimsley 11.33
Solomon 11.55
Lehninger 11.491
Nozaki 11.242
DTASelect 11.067
Thurlkill 11.242
EMBOSS 11.696
Sillero 11.242
Patrickios 11.316
IPC_peptide 11.55
IPC2_peptide 10.028
IPC2.peptide.svr19 8.693
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
40
0
40
8106
48
614
202.7
22.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.218 ± 0.486
0.123 ± 0.041
6.329 ± 0.528
5.095 ± 0.439
4.577 ± 0.217
6.662 ± 0.587
1.369 ± 0.22
7.143 ± 0.294
7.316 ± 0.447
8.315 ± 0.346
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.295 ± 0.141
6.686 ± 0.388
2.911 ± 0.217
4.589 ± 0.242
3.22 ± 0.216
7.562 ± 0.486
7.254 ± 0.386
6.711 ± 0.316
1.16 ± 0.138
4.466 ± 0.423
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here