Brassica campestris (Field mustard)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42060 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A397ZUM9|A0A397ZUM9_BRACM Uncharacterized protein OS=Brassica campestris OX=3711 GN=BRAA03T10816Z PE=4 SV=1
MM1 pKa = 7.75LDD3 pKa = 2.88IAYY6 pKa = 9.42EE7 pKa = 4.09LVGQATSNSLLMFCFCNLIIVIILTGSSKK36 pKa = 10.34PGSMDD41 pKa = 3.2SQDD44 pKa = 3.7YY45 pKa = 8.57NTFTSSVSFNNLASEE60 pKa = 4.69DD61 pKa = 4.16HH62 pKa = 7.06DD63 pKa = 4.46CCGDD67 pKa = 3.72DD68 pKa = 3.93HH69 pKa = 8.34DD70 pKa = 5.03HH71 pKa = 7.06EE72 pKa = 4.48EE73 pKa = 4.28MIVIDD78 pKa = 4.1VQDD81 pKa = 3.96EE82 pKa = 4.63SLTDD86 pKa = 3.77PSSIPDD92 pKa = 3.37EE93 pKa = 4.76YY94 pKa = 10.96EE95 pKa = 4.11DD96 pKa = 5.85KK97 pKa = 10.87EE98 pKa = 4.57SCHH101 pKa = 6.52CCHH104 pKa = 7.48DD105 pKa = 4.84DD106 pKa = 4.5DD107 pKa = 7.09DD108 pKa = 4.85EE109 pKa = 6.23EE110 pKa = 4.67EE111 pKa = 5.87DD112 pKa = 5.3EE113 pKa = 6.54DD114 pKa = 6.65DD115 pKa = 6.43DD116 pKa = 7.48DD117 pKa = 7.44DD118 pKa = 7.04DD119 pKa = 6.52DD120 pKa = 4.94EE121 pKa = 6.26SSEE124 pKa = 4.16ADD126 pKa = 3.41VEE128 pKa = 4.28EE129 pKa = 4.59EE130 pKa = 4.62EE131 pKa = 5.31EE132 pKa = 4.46DD133 pKa = 3.89DD134 pKa = 4.13EE135 pKa = 4.41LRR137 pKa = 11.84KK138 pKa = 9.81RR139 pKa = 11.84AEE141 pKa = 3.73EE142 pKa = 4.53FIAKK146 pKa = 9.51VNNEE150 pKa = 3.72WKK152 pKa = 10.27HH153 pKa = 4.88EE154 pKa = 3.97KK155 pKa = 10.3LRR157 pKa = 11.84ALNLVYY163 pKa = 10.83

Molecular weight: 18.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A398A7L2|A0A398A7L2_BRACM Uncharacterized protein OS=Brassica campestris OX=3711 GN=BRARA_B00948 PE=3 SV=1
MM1 pKa = 7.57ANSKK5 pKa = 10.51RR6 pKa = 11.84LFGVVRR12 pKa = 11.84RR13 pKa = 11.84NLLRR17 pKa = 11.84RR18 pKa = 11.84SPSSITTIRR27 pKa = 11.84SSIQQTTRR35 pKa = 11.84EE36 pKa = 4.13NIAAIKK42 pKa = 9.68IQAFFRR48 pKa = 11.84GHH50 pKa = 6.09LARR53 pKa = 11.84RR54 pKa = 11.84AFRR57 pKa = 11.84ALKK60 pKa = 10.68SLVKK64 pKa = 9.99LQAVARR70 pKa = 11.84GVLVRR75 pKa = 11.84RR76 pKa = 11.84QARR79 pKa = 11.84IALHH83 pKa = 6.34CMHH86 pKa = 7.36ALARR90 pKa = 11.84LQVRR94 pKa = 11.84VRR96 pKa = 11.84ARR98 pKa = 11.84QILSHH103 pKa = 6.56

Molecular weight: 11.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

40244

1816

42060

16253173

30

5374

386.4

43.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.496 ± 0.011

1.83 ± 0.006

5.351 ± 0.008

6.73 ± 0.016

4.204 ± 0.009

6.506 ± 0.012

2.31 ± 0.005

5.022 ± 0.009

6.303 ± 0.012

9.457 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.477 ± 0.005

4.285 ± 0.008

4.95 ± 0.013

3.479 ± 0.009

5.492 ± 0.01

9.104 ± 0.015

5.157 ± 0.008

6.78 ± 0.008

1.262 ± 0.004

2.804 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski