Bacillus phage PBP180

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4JMS4|R4JMS4_9CAUD Uncharacterized protein OS=Bacillus phage PBP180 OX=2558359 GN=PBP180_005 PE=4 SV=1
MM1 pKa = 7.43FPTVADD7 pKa = 4.17IKK9 pKa = 10.5QFWDD13 pKa = 3.45WQCYY17 pKa = 8.31GPEE20 pKa = 4.85DD21 pKa = 3.21IAFYY25 pKa = 11.25VSIGWISAEE34 pKa = 4.19DD35 pKa = 3.7YY36 pKa = 11.4QNITGEE42 pKa = 4.08IYY44 pKa = 10.29EE45 pKa = 4.2AA46 pKa = 4.39

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4JHG4|R4JHG4_9CAUD Holin OS=Bacillus phage PBP180 OX=2558359 GN=PBP180_0039 PE=4 SV=1
MM1 pKa = 7.55LLKK4 pKa = 10.85LLGQIAGRR12 pKa = 11.84KK13 pKa = 9.04NEE15 pKa = 3.76FDD17 pKa = 3.59SVVFTIKK24 pKa = 10.35EE25 pKa = 4.22GNRR28 pKa = 11.84PMQSIHH34 pKa = 6.73FIGVASIIGKK44 pKa = 8.38GRR46 pKa = 11.84RR47 pKa = 11.84DD48 pKa = 3.38KK49 pKa = 11.42LILL52 pKa = 3.91

Molecular weight: 5.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

40

0

40

8599

45

1288

215.0

24.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.605 ± 0.344

0.454 ± 0.082

5.966 ± 0.408

7.396 ± 0.541

3.78 ± 0.281

6.687 ± 0.587

1.803 ± 0.275

6.419 ± 0.255

8.094 ± 0.436

8.257 ± 0.356

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.838 ± 0.194

4.78 ± 0.391

3.419 ± 0.24

5.082 ± 0.341

4.733 ± 0.296

6.431 ± 0.498

6.233 ± 0.287

6.036 ± 0.404

1.582 ± 0.288

3.407 ± 0.415
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski