Capybara microvirus Cap1_SP_55
Average proteome isoelectric point is 5.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W4N1|A0A4P8W4N1_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_55 OX=2584783 PE=4 SV=1
MM1 pKa = 7.1 FRR3 pKa = 11.84 CRR5 pKa = 11.84 QPIRR9 pKa = 11.84 FSPSSSISLIEE20 pKa = 3.98 PSLVDD25 pKa = 3.8 DD26 pKa = 4.6 VYY28 pKa = 11.09 VCKK31 pKa = 10.51 EE32 pKa = 3.23 IDD34 pKa = 3.19 QCRR37 pKa = 11.84 SLPSSDD43 pKa = 4.54 NMDD46 pKa = 4.07 LKK48 pKa = 10.4 TLLDD52 pKa = 4.1 AGLPIDD58 pKa = 4.2 EE59 pKa = 4.69 VSTIIRR65 pKa = 11.84 SGSVDD70 pKa = 3.58 LSEE73 pKa = 4.27 LAKK76 pKa = 10.62 KK77 pKa = 10.72 YY78 pKa = 10.58 GVEE81 pKa = 3.89 IKK83 pKa = 10.84 NKK85 pKa = 9.61 EE86 pKa = 3.89 NEE88 pKa = 3.94
Molecular weight: 9.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.749
IPC2_protein 4.774
IPC_protein 4.609
Toseland 4.431
ProMoST 4.609
Dawson 4.558
Bjellqvist 4.774
Wikipedia 4.444
Rodwell 4.431
Grimsley 4.342
Solomon 4.546
Lehninger 4.507
Nozaki 4.66
DTASelect 4.838
Thurlkill 4.444
EMBOSS 4.457
Sillero 4.711
Patrickios 4.088
IPC_peptide 4.558
IPC2_peptide 4.698
IPC2.peptide.svr19 4.699
Protein with the highest isoelectric point:
>tr|A0A4P8W7G1|A0A4P8W7G1_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_55 OX=2584783 PE=4 SV=1
MM1 pKa = 7.41 NLNNFRR7 pKa = 11.84 VKK9 pKa = 10.39 SISPCSPKK17 pKa = 10.38 KK18 pKa = 10.86 VFVSNPRR25 pKa = 11.84 SYY27 pKa = 11.62 SSIKK31 pKa = 10.4 AKK33 pKa = 10.29 SDD35 pKa = 2.87 SWSFRR40 pKa = 11.84 LAAEE44 pKa = 4.58 LKK46 pKa = 9.46 NAVEE50 pKa = 4.21 NGYY53 pKa = 10.31 KK54 pKa = 10.28 VGFVTLTYY62 pKa = 11.03 NDD64 pKa = 4.4 DD65 pKa = 3.61 NLPYY69 pKa = 10.57 VDD71 pKa = 5.09 GSLLRR76 pKa = 11.84 DD77 pKa = 3.44 VTLDD81 pKa = 3.45 SRR83 pKa = 11.84 PCFDD87 pKa = 3.78 RR88 pKa = 11.84 KK89 pKa = 7.9 QVRR92 pKa = 11.84 LFVDD96 pKa = 5.23 NIRR99 pKa = 11.84 KK100 pKa = 8.41 WLWRR104 pKa = 11.84 HH105 pKa = 4.9 WRR107 pKa = 11.84 ITDD110 pKa = 3.31 IKK112 pKa = 9.5 YY113 pKa = 7.84 TVCSEE118 pKa = 3.88 YY119 pKa = 11.39 GEE121 pKa = 4.37 FTRR124 pKa = 11.84 RR125 pKa = 11.84 PHH127 pKa = 4.76 YY128 pKa = 10.14 HH129 pKa = 7.04 AVFAWPFKK137 pKa = 10.53 AASFYY142 pKa = 10.76 IGNDD146 pKa = 3.21 EE147 pKa = 4.1 NKK149 pKa = 10.31 KK150 pKa = 10.52 LVTRR154 pKa = 11.84 PPFSAAMFHH163 pKa = 7.03 ALIKK167 pKa = 10.51 RR168 pKa = 11.84 FWHH171 pKa = 6.07 YY172 pKa = 11.43 GFISPRR178 pKa = 11.84 DD179 pKa = 3.52 YY180 pKa = 11.26 RR181 pKa = 11.84 GGKK184 pKa = 7.38 SCKK187 pKa = 9.65 GRR189 pKa = 11.84 VYY191 pKa = 10.78 EE192 pKa = 4.25 PFEE195 pKa = 4.18 VDD197 pKa = 3.03 PLKK200 pKa = 11.2 VVGAACYY207 pKa = 9.58 CGKK210 pKa = 10.35 YY211 pKa = 9.0 CCKK214 pKa = 10.6 DD215 pKa = 2.73 IAFYY219 pKa = 11.16 EE220 pKa = 4.4 DD221 pKa = 4.68 LPTDD225 pKa = 3.67 YY226 pKa = 10.35 FVQSDD231 pKa = 3.82 DD232 pKa = 3.71 VDD234 pKa = 3.68 VKK236 pKa = 11.03 KK237 pKa = 10.68 AIKK240 pKa = 10.3 NCMPFHH246 pKa = 6.44 LHH248 pKa = 6.2 SKK250 pKa = 10.45 SLGLCFLDD258 pKa = 5.72 KK259 pKa = 10.1 ITNEE263 pKa = 3.79 DD264 pKa = 3.32 KK265 pKa = 11.28 LRR267 pKa = 11.84 FLSKK271 pKa = 9.64 GVQRR275 pKa = 11.84 IGSDD279 pKa = 3.01 KK280 pKa = 10.52 FVSLPIYY287 pKa = 9.68 YY288 pKa = 10.04 KK289 pKa = 11.0 NKK291 pKa = 9.79 ILFDD295 pKa = 3.7 TYY297 pKa = 11.65 YY298 pKa = 11.2 SFDD301 pKa = 4.04 SNGDD305 pKa = 3.25 RR306 pKa = 11.84 LVRR309 pKa = 11.84 RR310 pKa = 11.84 KK311 pKa = 10.01 ANKK314 pKa = 9.49 FFLDD318 pKa = 3.62 NYY320 pKa = 10.37 FEE322 pKa = 4.65 IYY324 pKa = 9.89 HH325 pKa = 6.6 IKK327 pKa = 8.75 FQAYY331 pKa = 8.95 KK332 pKa = 10.64 SVFAPLFDD340 pKa = 5.35 DD341 pKa = 5.56 SYY343 pKa = 11.06 LKK345 pKa = 10.94 NYY347 pKa = 9.53 IDD349 pKa = 5.65 DD350 pKa = 4.38 VYY352 pKa = 11.52 LSDD355 pKa = 3.88 LKK357 pKa = 10.87 EE358 pKa = 3.85 RR359 pKa = 11.84 RR360 pKa = 11.84 FNLGVGDD367 pKa = 4.35 LNSFTMMYY375 pKa = 10.41 LSYY378 pKa = 11.43 AFVDD382 pKa = 3.95 PNFICNDD389 pKa = 3.97 EE390 pKa = 4.45 IITWLMRR397 pKa = 11.84 YY398 pKa = 9.25 VPFYY402 pKa = 11.14 EE403 pKa = 3.88 NYY405 pKa = 8.94 KK406 pKa = 10.53 FKK408 pKa = 10.85 GIKK411 pKa = 9.98 FGVSFEE417 pKa = 4.03 MKK419 pKa = 9.94 TYY421 pKa = 10.52 FNNLNRR427 pKa = 11.84 LFYY430 pKa = 10.28 DD431 pKa = 3.48 HH432 pKa = 7.12 LVIFCNDD439 pKa = 3.76 SISDD443 pKa = 3.71 TRR445 pKa = 11.84 LDD447 pKa = 4.91 DD448 pKa = 3.47 YY449 pKa = 11.71 RR450 pKa = 11.84 KK451 pKa = 6.82 TQKK454 pKa = 10.62 VKK456 pKa = 10.98 DD457 pKa = 4.44 FYY459 pKa = 11.58 NQQ461 pKa = 2.84
Molecular weight: 54.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.499
IPC2_protein 8.463
IPC_protein 8.317
Toseland 8.726
ProMoST 8.814
Dawson 9.18
Bjellqvist 9.136
Wikipedia 9.341
Rodwell 9.282
Grimsley 9.151
Solomon 9.209
Lehninger 9.165
Nozaki 9.194
DTASelect 9.004
Thurlkill 9.077
EMBOSS 9.297
Sillero 9.268
Patrickios 4.139
IPC_peptide 9.209
IPC2_peptide 7.892
IPC2.peptide.svr19 7.731
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1820
88
751
364.0
41.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.495 ± 1.265
1.429 ± 0.473
7.253 ± 0.655
4.56 ± 0.846
5.824 ± 1.048
5.824 ± 0.875
1.923 ± 0.143
4.945 ± 0.43
6.374 ± 0.892
9.396 ± 0.737
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.143 ± 0.374
5.879 ± 0.359
4.341 ± 0.617
3.077 ± 0.875
4.615 ± 0.549
9.286 ± 0.662
4.67 ± 0.694
6.923 ± 0.474
1.264 ± 0.345
4.78 ± 0.956
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here