Escherichia phage forsur

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Bonnellvirus; unclassified Bonnellvirus

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9X5U6|A0A6B9X5U6_9CAUD Uncharacterized protein OS=Escherichia phage forsur OX=2696398 GN=forsur_34 PE=4 SV=1
MM1 pKa = 7.47ALVDD5 pKa = 3.57STAAEE10 pKa = 3.86RR11 pKa = 11.84AEE13 pKa = 5.0FVAACNMAAKK23 pKa = 9.82YY24 pKa = 10.73LNVWTEE30 pKa = 4.4DD31 pKa = 3.66YY32 pKa = 11.2FFDD35 pKa = 3.86TTNTTLVAAYY45 pKa = 8.66QAYY48 pKa = 6.92VTRR51 pKa = 11.84VSNALTALTNN61 pKa = 3.72

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9X3X6|A0A6B9X3X6_9CAUD Uncharacterized protein OS=Escherichia phage forsur OX=2696398 GN=forsur_45 PE=4 SV=1
MM1 pKa = 7.34SAIEE5 pKa = 4.02VALMLGCHH13 pKa = 6.26AGIGSLVIYY22 pKa = 10.32IVGAARR28 pKa = 11.84KK29 pKa = 9.9SYY31 pKa = 10.87DD32 pKa = 3.33RR33 pKa = 11.84RR34 pKa = 11.84TKK36 pKa = 10.67LL37 pKa = 3.44

Molecular weight: 3.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13084

37

1035

237.9

26.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.57 ± 0.581

1.131 ± 0.221

6.007 ± 0.283

6.023 ± 0.386

3.378 ± 0.193

7.819 ± 0.315

1.903 ± 0.197

4.593 ± 0.213

4.356 ± 0.331

8.231 ± 0.286

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.92 ± 0.219

4.196 ± 0.318

4.219 ± 0.33

4.807 ± 0.466

5.747 ± 0.307

5.633 ± 0.386

6.137 ± 0.516

7.184 ± 0.31

1.452 ± 0.145

3.692 ± 0.233
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski